Several studies have explored the utilization of soil microorganisms, to address the environmental issues associated with glyphosate use and enhance crop yields. In our investigation, screening on Agar plate and broth medium  Luria Bertani was carried out after isolating bacterial strains from rhizospheric agricultural soil in Mascara,  Algeria, to biodegrade glyphosate, following that by testing the Plant Growth-Promoting Rhizobacteria and evaluate the effects of glyphosate on these proprieties. Our findings indicate that five bacterial strains exhibited growth in the presence of glyphosate concentrations up to 25 mg/ml, beyond this concentration the strains have developed tolerance. Following a partial examination of the 16S rRNA sequences, the bacterial strains were identified as belonging to the genus of Enterobacter. After 10 days of incubation with the glyphosate, Phosphate solubilization decreased in broth and agar Pikovskaya medium and the bacterial strains synthetized less of indole-3-acetic acid compared to the control, indicating the impact of glyphosate on these outcomes, high concentration of glyphosate inhibited nitrogen fixation, and various doses of glyphosate were found to restrict the growth of biofilms in these strains. The results of HPLC examination of secondary metabolites revealed that the primary degradation products of glyphosate in all strains were Sarcosine and Glycine. So, it seemed that the strain could both biodegrade glyphosate and use it for growth ,while also possessing rhizobacteria properties that promote plant development, enabling the use of the strains in the bioremediation of glyphosate-contaminated soils.

Environmental threats from the accumulation of plastic trash are getting worse.  It is robust, lightweight, corrosion-resistant, affordable, and durable. Microorganisms play a significant role in protecting our environment by degrading plastic wastes that are harmful either naturally or by chemical modification.  The current study aims to investigate the biodegradation of synthetic polyethylene through the utilization of a laboratory bioreactor. Various types of additives were introduced to the soil samples before subjecting them to a 30-day UV treatment. The degradation of polyethylene was shown through a reduction in weight following a 24-week incubation period with certain bacterial strains. Experimental findings have revealed that models subjected to UV radiation exhibit the highest degree of vulnerability and degradation. Approximately 52% of polyethylene (PE) films underwent degradation when exposed to soil enhanced with peat moss. In contrast, only 40% and 45% of PE films were destroyed when subjected to garden soil that was untreated and treated with UV radiation, respectively. In contrast, the addition of husk resulted in a 48% to 53% reduction in weight for PE films that were buried for the same duration of the experiment.  The highest level of effectiveness was achieved by the disintegration of the plastic material that was introduced into the soil along with organic fertilizers, resulting in a value of 56.60%. The weight loss outcomes have been substantiated by the utilization of the Atomic Force Electron Microscope (AFM) images, which exhibited the highest magnitude in the experimental model using soil supplemented with fertilizers.