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Ageing processes and soil microbial community effects on the biodegradation of soil 13C-2,4-D nonextractable residues
2009
Lerch, Thomas | Dignac, Marie-France | Nunan, Naoise | Barriuso, Enrique | Mariotti, André, A. | Environnement et Grandes Cultures (EGC) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Biogéochimie et écologie des milieux continentaux (Bioemco) ; École normale supérieure - Paris (ENS-PSL) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)
The biodegradation of nonextractable residues (NER) of pesticides in soil is still poorly understood. The aim of this study was to evaluate the influence of NER ageing and fresh soil addition on the microbial communities responsible for their mineralisation. Soil containing either 15 or 90-day-old NER of C-13-2,4-D (NER15 and NER90, respectively) was incubated for 90 days with or without fresh soil. The addition of fresh soil had no effect on the mineralisation of NER90 or of SOM, but increased the extent and rate of NER15 mineralisation. The analyses of C-13-enriched FAME (fatty acids methyl esters) profiles showed that the fresh soil amendment only influenced the amount and structure of microbial populations responsible for the biodegradation of NER15. By coupling biological and chemical analyses, we gained some insight into the nature and the biodegradability of pesticide NER.
Show more [+] Less [-]Fate of CL-20 in sandy soils: Degradation products as potential markers of natural attenuation
2009
Monteil-Rivera, Fanny | Halasz, Annamaria | Manno, Dominic | Kuperman, Roman G. | Thiboutot, Sonia | Ampleman, Guy | Hawari, Jalal
Hexanitrohexaazaisowurtzitane (CL-20) is an emerging explosive that may replace the currently used explosives such as RDX and HMX, but little is known about its fate in soil. The present study was conducted to determine degradation products of CL-20 in two sandy soils under abiotic and biotic anaerobic conditions. Biotic degradation was prevalent in the slightly acidic VT soil, which contained a greater organic C content, while the slightly alkaline SAC soil favored hydrolysis. CL-20 degradation was accompanied by the formation of formate, glyoxal, nitrite, ammonium, and nitrous oxide. Biotic degradation of CL-20 occurred through the formation of its denitrohydrogenated derivative (m/z 393 Da) while hydrolysis occurred through the formation of a ring cleavage product (m/z 156 Da) that was tentatively identified as CH2N-C(N-NO2)-CHN-CHO or its isomer N(NO2)CH-CHN-CO-CHNH. Due to their chemical specificity, these two intermediates may be considered as markers of in situ attenuation of CL-20 in soil. Two key intermediates of CL-20 degradation are potential markers of its natural attenuation in soil.
Show more [+] Less [-]Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant
2009
Paton, G.I. | Reid, B.J. | Semple, K.T.
Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > βCD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.
Show more [+] Less [-]Phytoremediation potential of the novel atrazine tolerant Lolium multiflorum and studies on the mechanisms involved
2009
Merini, Luciano J. | Bobillo, Cecilia | Cuadrado, Virginia | Corach, Daniel | Giulietti, Ana M.
Atrazine impact on human health and the environment have been extensively studied. Phytoremediation emerged as a low cost, environmental friendly biotechnological solution for atrazine pollution in soil and water. In vitro atrazine tolerance assays were performed and Lolium multiflorum was found as a novel tolerant species, able to germinate and grow in the presence of 1 mg kg⁻¹ of the herbicide. L. multiflorum presented 20% higher atrazine removal capacity than the natural attenuation, with high initial degradation rate in microcosms. The mechanisms involved in atrazine tolerance such as mutation in psbA gene, enzymatic detoxification via P₄₅₀ or chemical hydrolysis through benzoxazinones were evaluated. It was demonstrated that atrazine tolerance is conferred by enhanced enzymatic detoxification via P₄₅₀. Due to its atrazine degradation capacity in soil and its agronomical properties, L. multiflorum is a candidate for designing phytoremediation strategies for atrazine contaminated agricultural soils, especially those involving run-off avoiding. Finding of a novel atrazine-tolerant species, as a potential candidate for phytoremediating herbicide-contaminated agriculture soils and elucidation of the mechanisms involved in tolerance.
Show more [+] Less [-]Effect of birch (Betula spp.) and associated rhizoidal bacteria on the degradation of soil polyaromatic hydrocarbons, PAH-induced changes in birch proteome and bacterial community
2009
Tervahauta, Arja I. | Fortelius, Carola | Tuomainen, Marjo | Akerman, Marja-Leena | Rantalainen, Kimmo | Sipilä, Timo | Lehesranta, Satu J. | Koistinen, Kaisa M. | Kärenlampi, Sirpa | Yrjälä, Kim
Two birch clones originating from metal-contaminated sites were exposed for 3 months to soils (sand-peat ratio 1:1 or 4:1) spiked with a mixture of polyaromatic hydrocarbons (PAHs; anthracene, fluoranthene, phenanthrene, pyrene). PAH degradation differed between the two birch clones and also by the soil type. The statistically most significant elimination (p <= 0.01), i.e. 88% of total PAHs, was observed in the more sandy soil planted with birch, the clearest positive effect being found with Betula pubescens clone on phenanthrene. PAHs and soil composition had rather small effects on birch protein complement. Three proteins with clonal differences were identified: ferritin-like protein, auxin-induced protein and peroxidase. Differences in planted and non-planted soils were detected in bacterial communities by 16S rRNA T-RFLP, and the overall bacterial community structures were diverse. Even though both represent complex systems, trees and rhizoidal microbes in combination can provide interesting possibilities for bioremediation of PAH-polluted soils. Birch can enhance degradation of PAH compounds in the rhizosphere.
Show more [+] Less [-]Interacting effects of sulphate pollution, sulphide toxicity and eutrophication on vegetation development in fens: A mesocosm experiment
2009
Geurts, Jeroen J.M. | Sarneel, Judith M. | Willers, Bart J.C. | Roelofs, Jan G.M. | Verhoeven, Jos T.A. | Lamers, Leon P.M.
Both eutrophication and SO₄ pollution can lead to higher availability of nutrients and potentially toxic compounds in wetlands. To unravel the interaction between the level of eutrophication and toxicity at species and community level, effects of SO₄ were tested in nutrient-poor and nutrient-rich fen mesocosms. Biomass production of aquatic and semi-aquatic macrophytes and colonization of the water layer increased after fertilization, leading to dominance of highly competitive species. SO₄ addition increased alkalinity and sulphide concentrations, leading to decomposition and additional eutrophication. SO₄ pollution and concomitant sulphide production considerably reduced biomass production and colonization, but macrophytes were less vulnerable in fertilized conditions. The experiment shows that competition between species, vegetation succession and terrestrialization are not only influenced by nutrient availability, but also by toxicity, which strongly interacts with the level of eutrophication. This implies that previously neutralized toxicity effects in eutrophied fens may appear after nutrient reduction measures have been taken. Interspecific competition, vegetation succession and terrestrialization in fens depend on the interacting effects of SO₄ pollution, sulphide toxicity and nutrient availability.
Show more [+] Less [-]Characterizing pesticide sorption and degradation in macro scale biopurification systems using column displacement experiments
2009
Wilde, Tineke De | Spanoghe, Pieter | Mertens, Jan | Sniegowksi, Kristel | Ryckeboer, Jaak | Jaeken, Peter | Springael, Dirk
The efficiency of biopurification systems to treat pesticide-contaminated water was previously studied in microcosms. To validate the obtained results, macrocosm systems were set-up. Four pesticides (linuron, isoproturon, bentazone, and metalaxyl) were continuously applied to ten different organic substrate mixes. Retention of the pesticides was similar and in some cases slightly lower in the macrocosms compared to the microcosms. Differences in retention between the different mixes were however minimal. Moreover, the classification of the retention strength of the pesticides was identical to that observed in microcosms: linuron > isoproturon > metalaxyl > bentazone. Monod kinetics were used to describe delayed degradation, which occurred for isoproturon, metalaxyl and bentazone. No breakthrough of linuron was observed, thus, this pesticide was appointed as the most retained and/or degraded pesticide, followed by isoproturon, metalaxyl and bentazone. Finally, most of the matrix mixes efficient in degrading or retaining pesticides were mixes containing dried cow manure. Transport of pesticides in macrocosm containing organic substrates.
Show more [+] Less [-]Sequential biodegradation of TNT, RDX and HMX in a mixture
2009
Sagi-Ben Moshe, S. | Ronen, Z. | Dahan, O. | Weisbrod, N. | Groisman, L. | Adar, E. | Nativ, R.
We describe TNT's inhibition of RDX and HMX anaerobic degradation in contaminated soil containing indigenous microbial populations. Biodegradation of RDX or HMX alone was markedly faster than their degradation in a mixture with TNT, implying biodegradation inhibition by the latter. The delay caused by the presence of TNT continued even after its disappearance and was linked to the presence of its intermediate, tetranitroazoxytoluene. PCR-DGGE analysis of cultures derived from the soil indicated a clear reduction in microbial biomass and diversity with increasing TNT concentration. At high-TNT concentrations (30 and 90 mg/L), only a single band, related to Clostridium nitrophenolicum, was observed after 3 days of incubation. We propose that the mechanism of TNT inhibition involves a cytotoxic effect on the RDX- and HMX-degrading microbial population. TNT inhibition in the top active soil can therefore initiate rapid transport of RDX and HMX to the less active subsurface and groundwater. TNT and its metabolites are cytotoxic for RDX and HMX-degrading bacteria in polluted soil.
Show more [+] Less [-]Competitive biodegradation of dichlobenil and atrazine coexisting in soil amended with a char and citrate
2009
Qiu, Yuping | Pang, Hui | Zhou, Zunlong | Zhang, Ping | Feng, Yucheng | Sheng, G Daniel
The role of char nutrients in the biodegradation of coexisting dichlobenil and atrazine in a soil by their respective bacterial degraders, DDN and ADP, was evaluated. Under growing conditions, their degradation in soil extract was slow with <40% and <20% degraded within 64 h, respectively. The degradation in extracts and slurries of char-amended solids increased with increasing char content, due to nutritional stimulation on microbial activities. By supplementing soil extract with various major nutrients, the measured degradation demonstrated that P was the exclusive limiting nutrient. The reduction in the degradation of coexisting dichlobenil and atrazine resulted apparently from the competitive utilization of P by DDN and ADP. With a shorter lag phase, ADP commenced growing earlier than DDN with the advantage of utilizing P first in insufficient supply. This resulted in an inhibition on the growth of DDN and thus suppression on dichlobenil degradation. Competitive utilization of char nutrients by bacterial degraders resulted in the preferential biodegradation of atrazine over dichlobenil in a soil containing a wheat-straw-derived char.
Show more [+] Less [-]Ageing processes and soil microbial community effects on the biodegradation of soil 13C-2,4-D nonextractable residues
2009
Lerch, T.Z. | Dignac, M.-F. | Nunan, N. | Barriuso, E. | Mariotti, A.
The biodegradation of nonextractable residues (NER) of pesticides in soil is still poorly understood. The aim of this study was to evaluate the influence of NER ageing and fresh soil addition on the microbial communities responsible for their mineralisation. Soil containing either 15 or 90-day-old NER of ¹³C-2,4-D (NER15 and NER90, respectively) was incubated for 90 days with or without fresh soil. The addition of fresh soil had no effect on the mineralisation of NER90 or of SOM, but increased the extent and rate of NER15 mineralisation. The analyses of ¹³C-enriched FAME (fatty acids methyl esters) profiles showed that the fresh soil amendment only influenced the amount and structure of microbial populations responsible for the biodegradation of NER15. By coupling biological and chemical analyses, we gained some insight into the nature and the biodegradability of pesticide NER. Ageing processes influence the NER mineralisation rate and the microbial population involved.
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