During hydrobiological investigations of the river Dulenska (Serbia, Yugoslavia) in June 1996, algological samples were taken at this river. In the algae community are found 34 taxa from two divisio: Bacillariophyta (28) and Chlorophyta (6). While qualitative composition of the algae colony was relativelly uniformed quantitative one was changeable along the course of the Dulenska river. By saprobiological analysis, it was found that the quality of water was changing along the course of the river. At the upper and middle course of river the water belonging to the second class. At lower course of the river (below Rekovac) water quality was getfing worse and it belonging to the third class.

The investigations have been done in Krstonosica shaft at 3 points, in February, May and October 1995, and in January and April 1996. They included algae, Rotatoria, Copepoda, Cladocera, and Hydracarina. The species from divisio Bacillariophyta were presented most among the algal species, with 56 species, then from divisio Chlorophyta, with 26 taxons, Euglenophyta 11, Cyanobacteria 9, Pyrrophyta 3, Xanthophyta 2 and Chrysophyta 1. Among animal groups the highest number belonged to the Rotatoria group, 111 taxons have been determined. The highest number was found during autumn season (86), then in spring (73), and only 14 species in winter. The species that mostly prevailed were phytophyl species. The Cladocera group was present with 18 taxons. The phytophyl species from overgrown plants again prevailed. The Copepoda group was present with 10 species and the phytophyl species were prevailed. According to saprobiological characteristics the greatest number belongs to beta-mezosaprobionts, then oligosaprobionts. A small number of species indicates to the eutrophication process and organic pollution of water.

Heavy metal pollution is widespread, and has an increasing trend in some countries and regions. It can be easily accumulated in plants, leading to plant species loss and affecting plant community composition. Artificial restoration can conserve plant diversity in contaminated soils and accelerate the recovery of polluted ecosystems. The application of nitrogen (N) and phosphorus (P) is inexpensive and convenient, which can increase the resistance of plants to adversity and promote the growth of plants in heavy metal polluted soils. In order to examine the effect of N and P nutrition on the conservation of plant community, we conducted a comparison experiment in greenhouse using soil with low N and P concentration, and set five treatments: C (soil with no heavy metals and fertilizer addition), H (soil with heavy metals addition but with no fertilizer), HN (soil with heavy metals and N addition), HP treatment(soil with heavy metals and P addition), HNP treatment (soil with heavy metals, N and P addition). Our results showed that heavy metal pollution reduced plant species by 300%, and significantly decreased plant diversity (P < 0.05). N addition increased the richness of plant species and increased the dominance of Euphorbia peplus, but had no significant effect on plant diversity and community structure, while reduced the evenness of plant species. P addition of HP and HNP treatments restored plant species richness and increased plant diversity under heavy metal pollution. The plant community structures of these two treatments were more similar to that of group C. Compared with N addition, P addition had a better performance to restoring the species composition and relative dominance of plant communities. Our results provided a guidance for the restoration of plant communities and the conservation of plant species in low N and P concentration soils with the context of heavy metal pollution.

Perennial plant communities in the proximity of metal smelters and refineries may receive substantial inputs of base metal particulate as well as sulphate from the co-emission of sulphur dioxide. The Ni refinery at Port Colborne (Canada) operated by Inco (now Vale Canada Ltd.) emitted Ni, Co and Cu, along with sulphur dioxide, between 1918 and 1984. The objectives were to determine if vascular plant community composition, including standing litter, in twenty-one woodlots on clay or organic soil, were related to soil Ni concentration which decreased in concentration with distance from the Ni refinery. The soil Ni concentration in the clay woodlots ranged from 16 to 4130 mg Ni/kg, and in the organic woodlots, ranged from 98 to 22,700 mg Ni/kg. The concentrations of Co and Cu in the soils were also elevated, and highly correlated with soil Ni concentration. In consequence, each series of woodlots constituted a ‘fixed ratio ray’ of metal mixture exposure. For each of the woodlots, there were 16 independent measurements of ‘woodlot status’ which were correlated with elevated soil Ni concentration. Of the 32 combinations, there were eight linear correlations with soil Ni concentration, considerably more than would be expected by chance alone at a p-value of 0.05. With the exception of mean crown rating for shrubs at the clay sites, the correlations were consistent with the hypothesis that increased soil metal concentrations would be correlated with decreased diversity, plant community health or fitness, and increased accumulation of litter. Only five of the eight linear correlations were from the organic woodlots, suggesting that the observations were not confounded with soil type nor range in soil metal concentrations.

We investigated the efficiency of various by-products (sugarbeet lime, biosolid compost and leonardite), based on single or repeated applications to field plots, on the establishment of a vegetation cover compatible with a stabilization strategy on a multi-element (As, Cd, Cu, Pb and Zn) contaminated soil 4–6 years after initial amendment applications. Results indicate that the need for re-treatment is amendment- and element-dependent; in some cases, a single application may reduce trace element concentrations in above-ground biomass and enhance the establishment of a healthy vegetation cover. Amendment performance as evaluated by % cover, biomass and number of colonizing taxa differs; however, changes in plant community composition are not necessarily amendment-specific. Although the translocation of trace elements to the plant biotic compartment is greater in re-vegetated areas, overall loss of trace elements due to soil erosion and plant uptake is usually smaller compared to that in bare soil.

Nitrogen deposition and tropospheric ozone are important drivers of vegetation damage, but their interactive effects are poorly understood. This study assessed whether long-term nitrogen deposition altered sensitivity to ozone in a semi-natural vegetation community. Mesocosms were collected from sand dune grassland in the UK along a nitrogen gradient (5–25 kg N/ha/y, including two plots from a long-term experiment), and fumigated for 2.5 months to simulate medium and high ozone exposure. Ozone damage to leaves was quantified for 20 ozone-sensitive species. Soil solution dissolved organic carbon (DOC) and soil extracellular enzymes were measured to investigate secondary effects on soil processes.Mesocosms from sites receiving the highest N deposition showed the least ozone-related leaf damage, while those from the least N-polluted sites were the most damaged by ozone. This was due to differences in community-level sensitivity, rather than species-level impacts. The N-polluted sites contained fewer ozone-sensitive forbs and sedges, and a higher proportion of comparatively ozone-resistant grasses. This difference in the vegetation composition of mesocosms in relation to N deposition conveyed differential resilience to ozone.Mesocosms in the highest ozone treatment showed elevated soil solution DOC with increasing site N deposition. This suggests that, despite showing relatively little leaf damage, the ‘ozone resilient’ vegetation community may still sustain physiological damage through reduced capacity to assimilate photosynthate, with its subsequent loss as DOC through the roots into the soil.We conclude that for dune grassland habitats, the regions of highest risk to ozone exposure are those that have received the lowest level of long-term nitrogen deposition. This highlights the importance of considering community- and ecosystem-scale impacts of pollutants in addition to impacts on individual species. It also underscores the need for protection of ‘clean’ habitats from air pollution and other environmental stressors.

A dynamic model of forest ecosystems was used to investigate the effects of climate change, atmospheric deposition and harvest intensity on 48 forest sites in Sweden (n = 16) and Switzerland (n = 32). The model was used to investigate the feasibility of deriving critical loads for nitrogen (N) deposition based on changes in plant community composition. The simulations show that climate and atmospheric deposition have comparably important effects on N mobilization in the soil, as climate triggers the release of organically bound nitrogen stored in the soil during the elevated deposition period. Climate has the most important effect on plant community composition, underlining the fact that this cannot be ignored in future simulations of vegetation dynamics. Harvest intensity has comparatively little effect on the plant community in the long term, while it may be detrimental in the short term following cutting. This study shows: that critical loads of N deposition can be estimated using the plant community as an indicator; that future climatic changes must be taken into account; and that the definition of the reference deposition is critical for the outcome of this estimate.

Deposition of reactive nitrogen (N) is a major threat to terrestrial ecosystems associated with impacts on ecosystem properties and functions including carbon (C) and nutrient stocks, soil water quality and nutrient retention. In the oceanic-alpine Racomitrium heath habitat, N deposition is associated with moss mat degradation and a shift from bryophyte to graminoid dominance. To investigate the effects of moss mat decline on C and N stocks and fluxes, we collected Racomitrium heath vegetation/soil cores from sites along a gradient of N deposition in the UK. Cores were maintained under controlled conditions and exposed to scenarios of current (8–40 kg N ha⁻¹ y⁻¹), reduced (8 kg N ha⁻¹ y⁻¹) and elevated (50 kg N ha⁻¹ y⁻¹) N deposition. Cores from high N deposition sites had smaller aboveground C and N stocks and, under current conditions, leached large amounts of inorganic N and had low soil water pH compared with low N deposition sites. With reduced N deposition there was evidence for rapid recovery of soil water quality in terms of reduced N leaching and small increases in pH. Under high N deposition, cores from low N deposition sites retained much of the applied N while those with a history of high N deposition leached large amounts of inorganic N. Carbon fluxes in soil water and net CO₂ fluxes varied according to core source site but were not affected by the N deposition scenarios. We conclude that C and N stocks and cycling in Racomitrium heath are strongly affected by long-term exposure to N deposition but that soil water quality may improve rapidly, if N deposition rates are reduced. The legacy of N deposition impacts on moss mat cover and vegetation composition however, mean that the ecosystem remains sensitive to future pulses in N input.

Arid and semi-arid ecosystems (aridlands) cover a third of Earth's terrestrial surface and contain organisms that are sensitive to low level atmospheric pollutants. Atmospheric nitrogen (N) inputs to aridlands are likely to cause changes in plant community composition, fire frequency, and carbon cycling and storage. However, few studies have documented long-term rates of atmospheric N inputs in aridlands because dry deposition is technically difficult to quantify, and extensive sampling is needed to capture fluxes with spatially and temporally heterogeneous rainfall patterns. Here, we quantified long-term spatial and temporal patterns of inorganic N deposition in protected aridland ecosystems across an extensive urban-rural gradient using multiple sampling methods. We compared long-term rates of N deposition from ion-exchange resin (IER) collectors (bulk and throughfall, 2006–2015), wet-dry bucket collectors (2006–2015), and dry deposition from the inferential method using passive samplers (2010–2012). From mixed approaches with IER collectors and inferential methods, we determined that 7.2 ± 0.4 kgNha⁻¹y⁻¹ is deposited to protected Sonoran Desert within metropolitan Phoenix, Arizona and 6.1 ± 0.3 kgNha⁻¹y⁻¹ in nearby desert ecosystems. Regional scale models overestimated deposition rates for our sampling period by 60% and misidentified hot spots of deposition across the airshed. By contrast, the easy-deployment IER throughfall collectors showed minimal spatial variation across the urban-rural gradient and underestimated deposition fluxes by 54%, largely because of underestimated dry deposition in throughfall. However, seasonal sampling of the IER collectors over 10 years allowed us to capture significant seasonal variation in N deposition and the importance of precipitation timing. These results, derived from the longest, spatially and temporally explicit dataset in drylands, highlight the need for long-term, mixed methods to estimate atmospheric nutrient enrichment to aridlands in a rapidly changing world.