During the Kharif season of 2022-2023 at Lovely Professional University, Jalandhar, Punjab, a field experiment was conducted to investigate the “Effect of soil and foliar application of humic acid and brassinolide on morpho-physiological and yield parameters of Black gram (Vigna mungo).” The experiment was designed using a Randomised Block Design (RBD) with three replications and eight treatments. Compared to the other treatments, RDF + humic acid 0.1% + brassinolide 0.1ppm (foliar application) was the optimal treatment for most morphological and yield parameters. Plant height (cm), number of primary branches per plant, number of secondary branches per plant, dry matter accumulation (g), chlorophyll Index (SPAD), and leaf area (cm2) were highest under T7- RDF + humic acid 0.1%+brassinolide 0.1ppm (foliar applied) conditions. Minimum phenological observations were recorded for soil and foliar applications of brassinolide, including days to first flowering, days to 50 percent flowering, and days to pod initiation. Number of pods /plant, pod length(cm), pod weight (g), no. of seeds /pod, test weight (g), seed yield (q/ha), stover yield (q/ha), and harvest index (%) were significantly influenced by the T7 and recorded higher values. The increased seed yield may be attributable to plants treated with growth regulators remaining physiologically more active to accumulate sufficient food reserves for developing blossoms and seeds.

Imidacloprid (IMI) is a commonly used pesticide against aphids and accumulates in plant parts, maximum in leaves. Present study was conducted to check the efficiency of seed pre-soaking with 24-epibrassinolide (24-EBL) for reduction of this pesticide in the leaves of Brassica juncea L. plants raised from 24-EBL pre-soaked seeds and grown in soils supplemented with IMI. Leaves were analyzed for IMI residues using gas chromatography-mass spectrometry (GC-MS). Activities of guaiacol peroxidase (POD), glutathione reductase (GR), and glutathione S-transferase (GST), and glutathione (GSH) content were determined by spectrophotometry. Soil containing 350 mg IMI/kilogram soil resulted in 88.66 μg/g fresh weight (FW) of IMI residue in the leaves, which was maximum decrease to 35.31 μg/g FW (60.17 %), when seeds were pre-soaked in 100 nM 24-EBL. In this treatment (350 mg IMI/kilogram soil + 100 nM 24-EBL), GSH content, GR, POD, and GST activities were increased by 42.30, 34.5, 20.5, and 13.4 %, respectively, as compared to plants grown in soils amended with 350 mg IMI/kilogram soil.