Heavy metals like cadmium (Cd), mercury (Hg), bismuth (Bi), and arsenic (As) are potent and harmful poisonous sources that cause havoc on health conditions for the population of the world. However, the response of our crop species to these potent heavy metals-related toxicity is still left to be fully understood. It is a matter of great concern, as we are heavily dependent on crop species like rice, wheat, peas, etc. Our study here aims to learn about the defensive mechanism of Pisum sativum L. aided with putrescine and mycorrhiza against the stress created by Cd-related toxicity. We quantified physiological parameters such as the membrane-related injury and stability index. We further measured the total free proline content, lipid peroxidation content, and SOD activity. We executed our quantitative experiments on the stressed pea plants due to the exogenously applied Cd-toxicity in the presence and absence of mycorrhiza and putrescine. Insights of our significant results will improve the understanding of readers of the role of mycorrhiza and putrescine in improvising the tolerance level of a pea plant over Cd-related toxicity.

Cadmium is one of the most carcinogenic and hazardous heavy metals on the earth for causes many serious diseases and disorders in the plant body. The presence of Cd in the soil is equally harmful to the production of rice crops and human beings. A pot experiment was conducted to analyze the consequences of cadmium-induced stress on the antioxidative defense system in rice plants. The assessment of antioxidative defense mechanism based on the cadmium-induced stress in the range of 100 to 300 ppm while the parameters, Chlorophyll Content Index (SPAD), nitrogen (%), relative water content (%), membrane stability index (%), proline content (μg.g-1), and catalase activity (nm H2O2 mg-1.min-1) were used. The highest reduction in the Chlorophyll Content Index (CCI), nitrogen (%), RWC (%), and MSI (%) was recorded at the highest concentrations of Cd Cl2 (300 ppm). However, at the same time, an increase in proline content (μg.g-1) and catalase activity (nm H2O2 mg-1.min-1) were also detected at all the intervals of the study. The activity of CCI, amino acid, and enzyme were presented in % increase/decrease over the control of Cd-induced stress in rice plants. The reduction (%) in CCI (SPAD) and RWC (%) was recorded maximum at 75 Days after transplanting (DAT), while nitrogen (%) and MSI (%) were recorded at 50 DAT. However, the increase (%) in proline and Catalase activity was maximum at 75 and 50 DAT.

This research was conducted in the waters of Malili River, East Luwu Regency, with 4 observation points in Malili River East Luwu Regency, namely: (a) Southeast Sulawesi Sub Das (Point 1) namely Pongkeru village bridge, Coordinate point 12126.69’8°” E; (b) Larona Sub Das Karebbe basin bridge (Point 2), Coordinate point 12115.09’9°” E; (c) The meeting point of Larona sub-dash and Pongkeru sub-dash (Point 3), coordinate point 12159.64’8°” E; (d) Upper Malili River, Malili village, Malili bridge (Point 4), Coordinate point 12147.20’5°” E. Metal concentration and distribution were analyzed descriptively with the help of images (maps), graphs, and tables. Differences in Pb, Cd, and Hg metal concentrations in sediments between point locations were tested using analysis of variance (ANOVA) through the SPSS version 22 program. The relation between grain size, organic matter, and Pb, Cd, and Hg metal concentrations was tested using linear correlation. The results showed that the sediment content of Pb and Cd metal concentrations at each point location did not exceed NOAA (1999) quality standards. In the sediment, Hg metal concentration exceeds the quality standards of NOAA (1999) at each point, namely point 1. Pongkeru 0.590 μg.g-1, point 2. Karebbe 0.229 μg.g-1, point 3. Kawasule 0.514 μg.g-1 and point 4. Malili 0.358 μg.g-1. The relation between sediment size and Pb, Cd, and Hg metal concentrations at each point location has a weak correlation. The relation does not significantly affect the content of heavy metals in the sediment. It may be due to other factors, such as the source of heavy metal pollutants in each different point location. The relation between organic matter and the concentration of Pb, Cd, and Hg metals at each point location has a weak correlation. The relation does not significantly affect the content of heavy metals in the sediment because it may be due to other factors, such as different sources of heavy metal pollutants in each point location.

Cadmium (Cd) is ubiquitous and an unessential trace element existing in the environment. Anthropogenic activities and applications of synthetic phosphate fertilizers greatly enhance the concentration of Cadmium in the environment, which proves to be carcinogenic. The long-term effects of heavy metals contamination on plants and animals have recently become a major public health concern. Thanks to the application of science and technology, new environmental initiatives can have a lower environmental impact significantly. The role of microbes is very well known and must be considered as potential pollutant removers. Microbial flora can remove heavy metals and oil from contaminated soil and water. In comparison to conventional techniques, bioremediation itself proved to be a more potent technique because the established mechanisms render it ineffective. Biotechnological advancements are inherently harmful to the environment because they have the potential to reduce metal pollution. Pollutants in the environment can be effectively removed using bioremediation. Both native and introduced species can thrive in a microorganism-friendly environment.