Constructed wetlands (CWs) are man-made systems designed to treat a range of residential, commercial, and industrial wastewaters. The objective of the study was to evaluate the efficiency of wastewater treatment systems using constructed wetlands. The effectiveness of removing chemical and physical pollutants was also evaluated. The setup consisted of a hybrid flow system composed of upflow constructed wetland and a horizontal flow constructed wetland connected in series that is used for primary treatment of the influent of domestic wastewater. Two systems were analyzed: one cultivated with the ornamental species Canna Indica, and one cultivated with the cattail Cymbopogon flexuosus. It consisted of two treatment sections consisting of two plant species Cymbopogon citratus (lemon grass – first CW) and Canna xalapensis Horan (Canna Indica – second CW). The water quality parameters i.e., BOD, COD, TSS were analyzed according to APHA (American Public Health Association) by daily sampling. The CW was monitored for the quality of wastewater inflows and outflows and nutrient accumulation in plants. Results showed that the maximum COD removal for Lemon Grass and Canna Indica beds were 75% and 70% respectively at 200mg/L COD loading in the CW setup over a six-month period respectively. The maximum BOD removal found in Lemon Grass and Canna Indica beds were 73% and 64% respectively at a feed concentration of 200mg/L COD. Both the CWs together as one unit showed similar rates of TSS removal irrespective of the type of wetland plant species and were more efficient in treating wastewater.

Constructed wetland (CWs) systems offer an economical alternative to wastewater (WW) treatment in developing countries. So this study investigated lab-scale hybrid constructed wetlands (HCWs) with plant species Canna indica and Typha latifolia in mono and mixed culture for removing organic matter and nutrients from municipal wastewater (MWW) under arid climatic conditions. A HCW system consists of a storage tank feeding four series of vertical flow constructed wetlands (VFCWs) followed by horizontal flow-constructed wetlands (HFCWs). The results indicate that the planted beds performed better in removing suspended solids (TSS) (89.93% by Typha latifolia), biochemical oxygen demand (BOD5) (95.01% by mixed-culture), chemical oxygen demand (COD) (90.77 by Typha latifolia), nitrite (NO2-) (89.99% by mixed-culture), ammonium nitrogen (NH4+) (99.98 % by mixed-culture), and orthophosphate (PO43-) (87.22% by Typha latifolia) as compared to the unplanted bed for the same parameters (87.85%, 92.87%, 77.35%, 85.30%, 99.75%,  and 80.95%), respectively. The nitrate (NO3−) concentration in the effluent recorded the highest increase in the VFCW unit planted with mixed culture from 0.44 to 0.999 mg/l and decreased in the second stage to 0.588 mg/l at the HCW outlet. The mean values of the testing parameters in different HCW systems were not significant between the mono and mixed culture (P > 0.05), with a significant difference (P <0.05) between the VFCWs and HFCWs. The finding of this study demonstrated that Canna indica and Typha latifolia have been effective in WW treatment by HCW systems.

The use of constructed wetland (CW) is a natural way of treating wastewater sustainably and economically. However, the implementation of these systems in freezing conditions is still a matter of research and development. The treatment capacity of CWs relies largely on the biological and biochemical processes which further depends on physical conditions such as temperature, solar radiations, etc. Application of wetland systems for treating wastewater faces many challenges in regions with cold climates, resolving which this review has been made. This paper presents a thorough understanding of the components of CWs and their role in contaminant removal. A comprehensive review of the different types of CWs has been done describing the treatment efficiency achieved by its implementation in the cold climate. Furthermore, various technologies which can be clubbed with CWs have also been listed along with the treatment efficiencies obtained. Literature survey indicates that the extent of removing organics (COD and BOD5) and total phosphorous (TP) are not likely to be affected, but total nitrogen (TN) removal appears to slow down at low temperatures. Despite several advantages of CW technology, further research is required to select suitable macrophytes and optimum design parameters to compensate for frigid conditions.

Emergent wetland plant species may exhibit different nutrient removal efficiencies when grown in monoculture and mixed stands in constructed wetlands for tertiary purification of wastewater. A glasshouse study was conducted to investigate the influence of mono- and mixed-culture between Canna indica Linn and Schoenoplectus validus (Vahl) A. Löve & D. Löve on their growth in, and nutrient removal from, simulated wastewater in the surface water of vertical-flow wetland microcosms. Plants were grown for 50 days before imposing nutrient treatments that simulated secondary-treated municipal wastewater effluent with either low (17.5 mg N and 10 mg P per litre) or high (35.0 mg N and 20 mg P per litre) nutrient concentrations. Treatment solutions were renewed in weekly intervals. After 65 days of nutrient and plant treatments, the total and above-ground biomass was significantly (P < 0.01) greater in the high compared with the low nutrient treatment, but there were no significant differences in below-ground biomass. Significant (P < 0.01) differences in above-ground and below-ground biomass were observed, but no significant difference in total biomass was detected among plant treatments. The highest below-ground biomass was in monoculture of C. indica, whereas the highest above-ground biomass was in the monoculture of S. validus. The biomass of mixed-culture was intermediate to that in the two monoculture treatments. There was significant interspecific competition between C. indica and S. validus in mixed-culture, with C. indica being the superior competitor. The concentrations of N and P in plant tissues (except P in above-ground tissues) were significantly (P < 0.01) higher in the high than in the low nutrient treatment. The accumulation of N and P in above- and below-ground tissues largely reflected patterns of biomass allocation. No significant difference was observed between the nutrient treatments in nutrient removal efficiencies. Plant uptake was the major nutrient removal pathway in the wetland microcosms. Nutrient removal from simulated wastewater in mixed-culture was not greater than in mono-cultures, due to interspecific competition. The results suggested that plant nutrient uptake was the major removal mechanism at the establishment stands in the constructed wetlands.

Aquatic plants can be used for in situ remediation of water-borne pharmaceutical compounds; however, such information and that of the potential risks of metabolites released into the environment are limited. This study determined the capacity of Canna indica and Acorus calamus used in the remediation of water-borne sulfonamides (SA). The tolerance, removal, accumulation, and biotransformation of various water-borne SAs were investigated in vivo by exposing plants to SA solutions (50 µg/L and 500 µg/L). After 28 days, C. indica removed more SAs (89.3–97.8%) than A. calamus (12.8–84.6%) and non-planted systems (8.0–69.3%). The SA removal results, except from the A. calamus system with 500 µg/L SA, fit the first-order kinetics model. The estimated half-lives of all SAs were 3–40 h and 2–60 h in the C. indica and A. calamus systems, respectively. In vivo biotransformation and rhizosphere degradation were the major phyto-removal mechanisms, constituting 24.9–81.1% and 0.0–37.1% of all SAs in the C. indica and A. calamus systems, respectively. SA acetyl metabolites were detected only in plant tissues supporting evidence for plant metabolic processes without risk into the environment. SA metabolism including oxidation, methylation, and conjugation via acetylation was potentially beneficial to accumulation and tolerate stress of antibiotic. Canna indica was more suitable for cleaning SA. Our findings better clarify the potential and low risks of phytoremediation in antibiotic-contaminated waters.

Air pollutant concentration of Trivandrum, the capital of Kerala, exceeded the limits of National Ambient Air Quality (NAAQ) standards, according to a study conducted in 2015 by NATPAC. These polluted corridors harbour vegetation on roadsides and traffic islands, planted solely for aesthetic appeal. Analysis of air pollution tolerance levels of existing plants can act as a scientific basis for efficient planning of the urban landscape. Sixty-seven species, including flowering, fruit-bearing, ornamental, shade-providing and timber-yielding species, were screened for their relative resistance to air pollution. Based on leaf pH, relative water content, chlorophyll and ascorbic acid levels, the Air Pollution Tolerance Indices (APTI) of each species were formulated and they were grouped into the following: tolerant, moderately tolerant, intermediate and sensitive groups. Agave americana (18.40), Cassia roxburghii (17.63), Anacardium occidentale (11.97), Cassia fistula (11.60), Mangifera indica (11.59) and Saraca asoca (10.88) may be considered for planting near green spaces like roundabouts and near pollution prone industrial areas, as they belong to tolerant category. Comparison of APTI during summer and monsoon also revealed the stability of Agave americana, Saraca asoca, Ficus benghalensis, Peltophorum pterocarpum, Ficus elastica, Ixora finlaysoniana, Mangifera indica, Canna indica and Delonix regia in maintaining pollution tolerance even during water disparity. Agave americana, Anacardium occidentale, Ficus elastica, Mangifera indica, Syzygium cumini, Ficus benghalensis, Nerium oleander and Ficus benjamina were found to be suited for mass planting, as was evident from their Anticipated Performance Indices (API).

Plant species diversity could enhance plant productivity and pollutant removal efficiency in constructed wetlands (CWs). However, the potential importance of plant density for ecosystem functioning has largely been neglected. In this study, we conducted a factorial experiment in which three common plant species were planted in a gradient of species richness (one, two, and three) and seven species compositions at two densities (six and twelve individuals per microcosm). Plant total biomass and total organic carbon (TOC) and total inorganic nitrogen (TIN) removal efficiency were measured to explore the effect of plant species diversity and density on the ecosystem functioning of CWs. Results showed that (1) plant species richness had no significant effect on plant total biomass and TOC and TIN removal efficiency under high and low plant density. (2) There were significant differences in TIN removal efficiency among seven species compositions under low plant density; especially, the presence of Canna indica reduced the TIN removal efficiency. In contrast, species composition and species identity had no significant effect on ecosystem functioning under high plant density. (3) High plant density increased plant total biomass of C. indica monocultures, and also enhanced TIN removal efficiency in mixtures of two species. These results indicated C. indica alone may not be an ideal species for enhancing pollutant removal in constructed wetlands but planting at high density could mitigate its negative effect on ecosystem functioning.

This study aims to evaluate the practical potential of using constructed wetlands (CWs) for treating saline wastewater containing various heavy metals. The results demonstrated that CWs growing Canna indica with porous slag as substrate could efficiently remove heavy metals (Cu, Zn, Cd, and Pb) from saline wastewater at an electrical conductivity (EC) of 7 mS/cm, especially under low influent load. Salts with salinity level (characterized as EC) of 30 mS/cm suppressed the removal of some heavy metals, dependent on heavy metal species and their influent concentrations. The presence of salts in CWs can improve the accumulation of Cu, Zn, and Pb in plant tissues as compared to control treatment, irrespective of metal concentrations in solution. The influence of salts on Cd accumulation depended on both salinity levels and Cd concentrations in solution. Although more heavy metals were accumulated in roots than in shoots, the harvesting of aboveground plant materials is still efficient addition for heavy metal removal due to the greater biomass and growth rate of aboveground plant material. Furthermore, replacing all plants instead of preserving roots from harvested plants in CWs over a period of time is essential for heavy metal removal, because the continued accumulation by roots can be inhibited by the increasing accumulated heavy metals from saline wastewater.

Ecological ditches have demonstrated the ability to filter and control nutrient transport to rivers. Few studies, however, have examined the internal loading of nitrogen (N) and phosphorus (P) in these systems due to vegetation decomposition. Most often, this concept is overlooked during evaluation of the nutrient removal rate of the ditches. Thus, the litter bag technique was used to analyze nutrient release to surface water during these processes. Mesocosm and field experiments were conducted to assess the growth characteristics and consequent nutrient accumulation by six ditch plant species. Of the six, Canna indica had the highest aboveground accumulation of N and P. About 85–95 % increase in the aboveground biomass was recorded at the end of the experimental period. The removal efficiencies of TN, TP, and NH₄-N from the sewage reached up to 72–99.4, 64–98.7, and 75 %–100, respectively. Complete removal of all NO₃-N was achieved. The amounts of N and P uptake by plant species were closely related to the biomass of plants. During the decaying process, N and P concentrations in the aboveground biomass decreased. These lost nutrients were eventually shifted to the system, which led to a deterioration of the water quality. Therefore, harvesting of aboveground biomass from inside the ditch is an appropriate intervention to prevent the release of N and P in the dormant season. The finding is important for planning an efficient eco-ditch system and predicting the influence of nutrient loading in the eco-ditches upon senescence of ditch plants.

We investigated the treatment efficiency of micro-polluted NO₃⁻-dominated river water with low C/N ratio by five parallel pilot-scale IVCWs with different plant and substrate collocation. When the mean concentration was 2.24 and 0.193 mg L⁻¹ in influent, IVCWs achieved an average (mass) removal rate of (0.09 g m⁻² day⁻¹) 46.8% and (0.77 g m⁻² day⁻¹) 62.3% for TN and TP, respectively, during 1 year of operation. Water quality was significantly improved from grade V to meet the criterion of grade IV of surface water. Through the comparison of removal rate by different IVCWs, we found that lack of carbon sources in influent limited the denitrification in the middle and bottom layers (ML, BL) of IVCW. Zeolites deployed in the upper layer (UL) of IVCW reduced the overall N removal efficiency compared with gravels, due to a stronger nitrification but weaker denitrification. Canna indica (C. indica) was superior to Arundo donax (A. donax) and Thalia dealbata (T. dealbata) for N removal in the UL of IVCW due to higher aboveground biomass accumulation and microbial removal during the first 10 months. Stronger nitrification and denitrification were simultaneously facilitated near the rhizosphere of C. indica. When entered into Dec., A. donax performed higher N removal efficiency than the other two species. The internal replenishment of peats in the ML as carbon sources significantly improved N and P removal efficiency. Zeolites with stronger capacity of ammonium (NH₄⁺) adsorption was more in favor of anammox in the BL, when compared with roseites, but both of them were not conducive to the growth of denitrifiers. However, the deployment of shale ceramisites obtained an opposite result. Gemmata and Pirellula as anammox bacteria were more enriched in the zeolite layer, whereas some anaerobic denitrifiers (Corynebacterium and Paludibacter) and heterotrophic denitrifiers including Bacillus, Geobacter, Pseudomonas, and Lactococcus were more found in shale ceramisite. Supply of peats as carbon sources in the ML was beneficial for the adhesion of anammox bacteria and denitrifiers in the BL of shale ceramisites. An ideal model composed of C. indica + A. donax (DFU)-gravel (UL)-anthracite+peat (ML)-zeolite+shale ceramsite (BL)-Acorus calamus (UFU) was proposed for treating this type of river water to achieve high efficiency.