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Metal(loid) oxides and metal sulfides nanomaterials reduced heavy metals uptake in soil cultivated cucumber plants Full text
2019
Song, Chun | Ye, Fang | Zhang, Huiling | Hong, Jie | Hua, Chenyu | Wang, Bin | Chen, Yanshan | Ji, Rong | Zhao, Lijuan
Agricultural soil is one of the main sink for both heavy metals and nanomaterials (NMs). Whether NMs can impact heavy metals uptake or bioaccumulation in plants is unknown. Here, cucumber plants were cultivated in a multi-heavy metals contaminated soil amended with four types of NMs (SiO2, TiO2, ZnS and MoS2) separately for four weeks. Physiological and biochemical parameters were determined to investigate the impact of NMs on plant growth. Inductively coupled plasma mass spectrometry was employed to determine the metal content in plants. Results showed that none of the tested NMs impacted plants biomass, but all the NMs showed different degrees of reduction in heavy metals bioaccumulation in plant roots, stems and leaves. However, four NMs showed different degrees of reduction in macro and micro nutrients uptake. MoS2 decreased the bioaccumulation of heavy metals (As, Cd, Cr, Cu, Ni, Al, Ti and Pb) for 36.4–60.6% and nutrients (Mg, Fe, K, Si and Mn) for 40.1%–50.1% in roots. Exposure to MoS2 NMs also significantly increased 23.4% of Si in leaves, 205.6% and 83.9% of Mo in roots and stems, respectively. In general, the results of this study showed promising potential for NMs to reduce uptake of heavy metals in crop plants, especially MoS2 NMs. However, the negative impacts of perturbing nutrients uptake should be paid attention as well.
Show more [+] Less [-]Effect of gibberellic acid on growth, photosynthesis and antioxidant defense system of wheat under zinc oxide nanoparticle stress Full text
2019
Iftikhar, Azka | Ali, Shafaqat | Yasmeen, Tahira | Arif, Muhammad Saleem | Zubair, Muhammad | Rizwan, Muhammad | Alhaithloul, Haifa Abdulaziz S. | Alayafi, Aisha A.M. | Soliman, Mona H.
The production and soil accumulation of nanoparticles (NPs) from the industrial sector has increased concerns about their toxic effects in plants which needs the research to explore the ways of reducing NPs toxicity in pants. The gibberellic acid (GA) has been found to reduce abiotic stresses in plants. However, the effect of GA in reducing zinc oxide (ZnO) NPs-mediated toxicity in plants remains unclear. In this study, foliar application of GA was used to explore the possible role in reducing ZnO NPs toxicity in wheat (Triticum aestivum L.) plants. The plants were grown in pots spiked with ZnO NPs (0, 300, 600, 900, 1200 mg/kg) and GA (0, 100, 200 mg/L) was foliar sprayed at different times during the growth period under ambient environmental conditions. Our results demonstrated that GA inhibited the toxicity of ZnO NPs in wheat especially at higher levels of NPs. The GA application improved the plant biomass, photosynthesis, nutrients, and yield under ZnO NPs stress. The GA reduced the Zn accumulation, and reactive oxygen species generation in plants caused by toxicity of NPs. The protective effect of GA in decreasing ZnO NPs-induced oxidative stress was related to GA-mediated enhancement in antioxidant enzymes in plants. The role of GA in enhancing tolerance of wheat against ZnO NPs was further confirmed by the enhancement in nutrient contents in shoots and roots of wheat. Overall, our study provides the evidence that GA can reduce ZnO NPs-induced toxicity in wheat and probably in other crops which needs further in-depth investigation.
Show more [+] Less [-]Uptake, translocation and distribution of three veterinary antibiotics in Zea mays L Full text
2019
Zhang, Cheng | Xue, Jianming | Cheng, Dengmiao | Feng, Yao | Liu, Yuanwang | Aly, Hesham M. | Li, Zhaojun
Frequently detected residuals of antibiotics in crops has drawn increasing attention from research community and the general public. This study was conducted under the controlled environmental conditions to investigate the uptake, translocation and distribution of three different veterinary antibiotics (VAs) in plants of Zea mays L. (maize, the third largest crop in the world, especially in China) and the associated mechanisms. The distribution color-maps of mixed-VAs showed that the highest RCF (root concentration factors) values of chlortetracycline (CTC) and sulfamethoxazole (SMZ) were found in the 0.5–2.0 mm zone (cell division zone), while the highest RCF value of sulfathiazole (ST) was in the 6.0–8.0 mm zone (elongation zone) of root tips (0.5–10.0 mm) after 120 h of exposure to VAs. The translocation factor (TF) of CTC was greater than 1.0, but the TFs of SMZ and ST were less than 1.0 under addition of single antibiotic. However, the TFs of three VAs were all greater than 1.0 at the end of exposure under addition of mixed-VAs. The dissipation of antibiotics by maize was also demonstrated by harvesting all plant parts in an enclosed system. The possible mechanisms for uptake and translocation of VAs in maize were investigated by adding multiple respiration inhibitors into the culture solution. The RCFs of VAs were suppressed heavily by salicylhydroxamic acid (SHAM) and sodium azide (NaN3), which indicates that the uptake of VAs was an active process. The results of TFs and stem concentration factors (SCFs) of CTC and SMZ in HgCl2 treatments revealed that the translocation of VAs was associated with the aquaporin activity in maize. The findings from this study will have significant implications for the management of crop food contamination by VAs and for the development of phytoremediation technology for antibiotics in the environment.
Show more [+] Less [-]Early life exposure to di(2-ethylhexyl)phthalate causes age-related declines associated with insulin/IGF-1-like signaling pathway and SKN-1 in Caenorhabditis elegans Full text
2019
How, Chun Ming | Yen, Pei-Ling | Wei, Chia-Cheng | Li, Shang-Wei | Liao, Vivian Hsiu-Chuan
Di(2-ethylhexyl)phthalate (DEHP) is an ubiquitous and emerging contaminant that is widely present in food, agricultural crop, and the environment, posing a potential risk to human health. This study utilized the nematode Caenorhabditis elegans to decipher the toxic effects of early life exposure to DEHP on aging and its underlying mechanisms. The results showed that exposure to DEHP at 0.1 and 1.5 mg/L inhibited locomotive behaviors. In addition, DEHP exposure significantly shortened the mean lifespan of the worms and further adversely affected pharyngeal pumping rate and defecation cycle in aged worms. Moreover, DEHP exposure also further enhanced accumulation of age-related biomarkers including lipofuscin, lipid peroxidation, and intracellular reactive oxygen species in aged worms. In addition, exposure to DEHP significantly suppressed gene expression of hsp-16.1, hsp-16.49, and hsp-70 in aged worms. Further evidences showed that mutation of genes involved in insulin/IGF-1-like signaling (IIS) pathway (daf-2, age-1, pdk-1, akt-1, akt-2, and daf-16) restored lipid peroxidation accumulation upon DEHP exposure in aged worms, whereas skn-1 mutation resulted in enhanced lipid peroxidation accumulation. Therefore, IIS and SKN-1 may serve as an important molecular basis for DEHP-induced age-related declines in C. elegans. Since IIS and SKN-1 are highly conserved among species, the age-related declines caused by DEHP exposure may not be exclusive in C. elegans, leading to adverse human health consequences due to widespread and persistent DEHP contamination in the environment.
Show more [+] Less [-]COMT1 overexpression resulting in increased melatonin biosynthesis contributes to the alleviation of carbendazim phytotoxicity and residues in tomato plants Full text
2019
Yan, Yanyan | Sun, Shasha | Zhao, Ning | Yang, Wanying | Shi, Qinghua | Gong, Biao
Melatonin (Mel) serves as an important signalling molecule in various aspects of stress tolerance in plants. However, the function of Mel in pesticide metabolism remains unknown. Here, selecting the widely used fungicide carbendazim (MBC) as the model, we found that exogenous Mel had the ability to alleviate pesticide phytotoxicity and residues in tomato as well as in some other vegetables. Additionally, overexpression of the Mel biosynthetic gene caffeic acid O-methyltransferase 1 (COMT1) significantly enhanced the capacity of the tomato to reduce MBC phytotoxicity and residue. This outcome was mainly because of the Mel-induced antioxidant capability, as well as the key detoxification process. Indeed, levels of reactive oxygen species (ROS) and lipid peroxides significantly decreased after applying exogenous Mel or overexpressing COMT1, which resulted from direct ROS scavenging, and increased Mel levels significantly enhanced antioxidant enzymatic activity. More importantly, Mel activated the ascorbate-glutathione cycle to participate in glutathione S-transferase-mediated pesticide detoxification. A grafting experiment showed that rootstocks from COMT1 transgenic plants increased the Mel accumulation of wild-type scions, resulting in MBC metabolism in the scions. To our knowledge, this is the first report providing evidence of Mel-induced pesticide metabolism, which provides a novel approach for minimizing pesticide residues in crops by exploiting plant self-detoxification mechanisms.
Show more [+] Less [-]Transformation of lamotrigine by white-rot fungus Pleurotus ostreatus Full text
2019
Chefetz, Benny | Marom, Rotem | Salton, Orit | Oliferovsky, Mariana | Mordehay, Vered | Ben-Ari, Julius | Hadar, Y. (Yitzhak)
One of the most persistent pharmaceutical compounds commonly found in treated wastewater is lamotrigine (LTG). It has also been detected in soils and crops irrigated with treated wastewater. Here we focused on the ability of the white-rot edible mushroom Pleurotus ostreatus to remove and transform LTG in liquid cultures. At concentrations of environmental relevance (1 and 10 μg L−1) LTG was almost completely removed from the culture medium within 20 days. To elucidate the mechanism of LTG removal and transformation, we applied a physiological-based approach using inhibitors and a competing agent. These experiments were conducted at a higher concentration for metabolites detection. Based on identification of sulfur-containing metabolites and LTG N2-oxide and the effect of specific inhibitors, cytochrome P450 oxidation is suggested as one of the reaction mechanisms leading to LTG transformation. The variety and number of transformation products (i.e., conjugates) found in the current study were larger than reported in mammals. Moreover, known conjugates with glucuronide, glutathione, or cysteine/glycine, were not found in our system. Since the majority of the identified transformation products were conjugates of LTG, this study highlights the persistence of LTG as an organic pollutant in ecosystems exposed to wastewater.
Show more [+] Less [-]Legal measures to prevent and manage soil contamination and to increase food safety for consumer health: The case of Spain Full text
2019
Ramón, Francisca | Lull, Cristina
This article contains a brief overview of the European and Spanish environmental law framework for the prevention of soil contamination, for the management of contaminated soils and for consumers health protection in relation to agricultural crops. Some important aspects of the legislative framework for the prevention and management of soil contamination include recognising the possible risk to both human health and ecosystems that certain agricultural and industrial activities pose given the use of organic and inorganic chemical substances of a hazardous nature and pathogenic microorganisms. It is worth highlighting the milestone that many national constitutions include about the right to the environment. This right entails the obligation to protect it and to, therefore, protect soil from any degradation, including contamination. Legislation that protects soil from contamination and, consequently human health and ecosystems, is related mainly to agricultural activities (use of sewage sludge on farmlands, use of wastewater for irrigation, use of organic fertilisers and pesticides), and to industrial and commercial soil-contaminating activities. Consumer protection may be achieved through a legal system of environmental liability, specific measures to prevent contaminants entering soil, managing contaminated soils and a food traceability system. It is crucial to make the penalties for soil contamination offenses, and for violators of protective prohibitions, effective, proportionate and dissuasive. Global standards and guidelines on soil contamination could provide national legislative systems with substantive and procedural legal mechanisms to help prevent and manage soil contamination.
Show more [+] Less [-]Source apportionment of heavy metal and their health risks in soil-dustfall-plant system nearby a typical non-ferrous metal mining area of Tongling, Eastern China Full text
2019
Wang, Juan | Su, Jingwen | Li, Zhonggen | Liu, Bingxiang | Cheng, Guanghua | Jiang, Yuehua | Li, Yucheng | Zhou, Shaoqi | Yuan, Wenyi
The agricultural land-atmospheric dustfall-plant system around the mining area is at high risks of heavy metal pollution caused by mining-smelting activities. In this study, 118 samples (including rhizospheric soils, background soils, soil-forming parent rocks, crops, vegetables, medicinal plants and atmospheric dustfall) were collected nearby Tongling Cu-Fe-Au mining area, Eastern China. We studied the concentrations, migration, sources, and health risks through consumption of two main crops (corn and rice), six kinds of vegetables, and medicinal plants (Fengdan, Paeonia ostii) for six metal elements (Cu, Zn, Cr, Cd, Pb and Hg). Results revealed Cr and Cd in soils, and Cd, Cr, Pb, Cu and Zn in dustfall showed a relatively high contamination degree. The mean contents of Cr and Pb in corn kernels, as well as Cd, Cr and Pb in rice grains and all vegetables, and Cr in Fengdan cortex moutan exceeded the corresponding food safety limits in China. The transfer capability of Cr in corn kernels and rice grains, Pb in edible vegetables, and Cd in cortex moutan were the strongest, respectively. Health risk assessment results showed Cr had the greatest non-carcinogenic risk, followed by Pb and Cd. The results of pearson's correlation analysis (CA), hierarchical cluster analysis (HCA), and principal component analysis (PCA) indicated Zn-Cr, Pb and Cd-Cu-Hg in the plants might derive from different geochemical end-members. Source apportionment based on lead isotope showed that mining-smelting activities were the major source of Pb in atmospheric dustfall and agricultural soils, with the average contribution rates of 66% and 50%, respectively. Vehicle emissions from diesel fuels (50%–68%) and mining-smelting activities (16%–25%) contributed mainly to Pb accumulation in plants. Hence, our study suggested the accumulations of Pb in plants might be mainly from the direct foliar uptake of atmospheric Pb related to vehicle emissions and mining-smelting activities.
Show more [+] Less [-]Identification of transformation products of carbamazepine in lettuce crops irrigated with Ultraviolet-C treated water Full text
2019
Martínez-Piernas, A.B. | Nahim-Granados, S. | Polo-López, M.I. | Fernández-Ibáñez, P. | Murgolo, S. | Mascolo, G. | Agüera, A.
Transformation of organic microcontaminants (OMCs) during wastewater treatments results in the generation of transformation products (TPs), which can be more persistent than parent compounds. Due to reuse of reclaimed wastewater (RWW) for crop irrigation, OMCs and TPs are released in soils being capable to translocate to crops. Furthermore, OMCs are also susceptible to transformation once they reach the soil or crops. The recalcitrant antiepileptic carbamazepine (CBZ) and some of its frequently reported TPs have been found in agricultural systems. However, there is no knowledge about the fate in reuse practices of multiple CBZ TPs that can be formed during wastewater treatment processes. For the first time, this work presents a study of the behavior of CBZ TPs generated after a conventional Ultraviolet-C (UVC) treatment in an agricultural environment. The UVC-treated water was used for the irrigation of lettuces grown under controlled conditions. The latter was compared to the fate of TPs generated in the peat and plant by irrigation with non-treated water containing CBZ. A suspect screening strategy was developed to identify the TPs using liquid chromatography coupled to quadrupole-time-of-flight (LC-QTOF-MS). The results revealed the presence of 24 TPs, 22 in UVC-treated water, 11 in peat and 9 in lettuce leaves. 4 of the TPs identified in peat (iminostilbene, TP 271B, TP 285A-B); and 3 in leaves (10–11 dihydrocarbamazepine, TP 271A-B) were not previously reported in soils or edible parts of crops, respectively. Comparing the TPs found in peat and lettuces derived from both irrigation conditions, no significant differences regarding TPs formation or occurrence were observed. UVC treatment did not contribute to the formation of different TPs than those generated by transformation or metabolism of CBZ in peat or plant material. This research improves the current knowledge on the fate of CBZ TPs in agricultural systems because of reuse practices.
Show more [+] Less [-]Fast visualization of distribution of chromium in rice leaves by re-heating dual-pulse laser-induced breakdown spectroscopy and chemometric methods Full text
2019
Peng, Jiyu | He, Yong | Zhao, Zhangfeng | Jiang, Jiandong | Zhou, Fei | Liu, Fei | Shen, Tingting
Knowledge of distribution of toxic metal in crop is essential for studying toxic metal uptake, transportation and bioaccumulation, and it is important for environmental pollution monitoring. In this study, the macro spatial distribution of chromium in rice leaves was visualized by re-heating dual-pulse laser-induced breakdown spectroscopy (DPLIBS) and chemometric methods. After the optimization of two important parameters (delay time and energy ratio) in DPLIBS, chromium prediction model was established based on global spectra. The global model achieved acceptable performance while slight overfitting for model was found because of numerous irrelevant variables. Feature variables including emissions from chromium and other elements were successfully selected by the values of regression coefficient in partial least square regression model. Best performance was achieved by using the feature variables and support vector machine, with correlation coefficient of prediction of 0.959, root mean square error of prediction of 13.4 mg/kg and residual predictive deviation of 3.6. Finally, the distribution of chromium in rice leaves was visualized with the best prediction model. The distribution image showed that chromium distributed approximately symmetrically along the vein and was likely to be accumulated in leaf apex. The preliminary results provide an approach for investigating the macro spatial distribution of elements in crops, which is important for environmental protection and food safety.
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