The majority of consumer products used today are comprised of some form of plastic. Worldwide, almost 280 million t of plastic materials are produced annually, much of which ends up in landfills or the oceans (Shaw and Sahni Journal of Mechanical and Civil Engineering 46–48, 2014). While plastics are lightweight, inexpensive, and durable, these same qualities can make them very harmful to wildlife, especially once they become waterborne. Once seaborne, plastics are most likely found circulating in one of five major ocean gyres: two in the Pacific, one in the Indian, and two in the Atlantic. These ocean garbage patches are not solid islands of plastic; instead, they are a turbid mix of plastics (Kostigen 2008; Livingeco 2011). Recent research conducted on the surfaces of the Great Lakes has identified similar problems (Erikson et al. Marine Pollution Bulletin, 77(1), 177–182, 2013). A growing concern is that once plastics reach the wild, they may cause entanglement, death from ingestion, and carry invasive species. Several cutting edge technologies have been piloted to monitor or gather the plastics already in our environments and convert them back into oil with hopes to reduce the damage plastics are causing to our ecosystems.

Walnut husk washing waters (WHWW), a by-product of walnut production, are indiscriminately used for irrigation without preliminary risk assessment. Basing on previous in vitro results on the toxicity of this by-product, we have followed the morphophysiological development of Zea mays, Lactuca sativa cv. Gentilina and L. sativa cv. Canasta under diluted and undiluted WHWW irrigation. Significant development alterations have been observed in root and shoot elongations for all crops as well as in total biomass and chlorophyll content. The genotoxic potential of WHWW has been concurrently verified; acridine orange/ethidium bromide staining evidenced chromatin modifications and DNA degradation and also was confirmed by DNA laddering. The DNA instability was also assessed through RAPD, thus suggesting the danger of the by-product of walnut processing and focusing the attention on the necessity of an efficient treatment of WHWWs. The findings obtained by PCA of agronomic and physiological traits suggested that establishing guidelines for the administration of WHWW for irrigation is of great importance, and it is necessary to supervise their use in agricultural soils.

The aim of the present study was to comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from crack cocaine users by micronucleus test. A total of 30 crack cocaine users and 30 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mechanically exfoliated, placed in fixative, and dropped in clean slides, which were checked for the above nuclear phenotypes. The results pointed out significant statistical differences (p < 0.05) of micronucleated oral mucosa cells from crack cocaine users. Exposure to crack cocaine caused an increase of other nuclear alterations closely related to cytotoxicity such as karyolysis in oral cells as well. In summary, these data indicate that crack cocaine is able to induce chromosomal breakage and cellular death in oral mucosa cells of users.