To improve total nitrogen removal, a full-scale experimental study was conducted on a hybrid constructed wetlands plant designed for 100 p.e.. The plant composed of a 1st stage of vertical filters (fed with raw wastewater) followed by a 2nd stage of horizontal filters was monitored over one year measuring hydraulic conditions, physico-chemical conditions, gas emission, oxygen of the gas phase and regular treatment performances by 24 h composite samples. Different vertical filter configurations (media depth, intermediate and passive aeration system) were tested as well as two horizontal filter designs. The N removal is discussed on efficiency of each stage in relation to the season and the load applied. This study leads to draw the limit of the plant configuration and gives some design avenues of hybrid systems to reach high and regular level of nitrogen removal with acceptable surface area per person.

The overall objective of this research was to develop a reliable, robust, and maintenance-free passive system for biological denitrification in on-site wastewater treatment systems. The process relies on sulfur oxidizing denitrifying bacteria in upflow packed bioreactors. Since this process consumes alkalinity, it is necessary to add a solid-phase buffer that can scavenge the H⁺ as it is generated by the biologically-mediated reaction and arrest the drop in the pH value. This study investigated the use of limestone, marble chips and crushed oyster shell as solid-phase buffers that provide alkalinity. Two bench-scale upflow column reactors and two field-scale bioreactors were constructed and packed with sulfur pellets and an alkalinity source. The pilot scale bioreactors (~200 L each) were installed at the Massachusetts Alternative Septic System Test Center (MASSTC) in Sandwich, MA. The pilot-scale bioreactors performed better when oyster shell was used as the solid-phase buffer vis-à-vis marble chips. In both (pilot-scale and laboratory-scale) systems, denitrification rates were high with the effluent NO₃ - --N concentration consistently below 8 mg/L.