This study aimed to assess the efficacy of a bimetallic system consisting of Mg0-Pd0 and the bacterium Acinetobacter sp. for the complete detoxification of lindane. Our results demonstrate that palladium immobilized on activated charcoal achieved a removal rate of >99% for 100 mg.L-1 of Lindane within 10 minutes, with the accumulation of trace amounts of intermediates. The reductive transformation of lindane followed 1st-order kinetics, with a calculated rate constant (kobs) of 0.77 min-1. The bimetallic system resulted in the formation of a non-toxic hydrocarbon as the end-product, indicating complete dehalogenation of lindane. Furthermore, Acinetobacter sp. effectively mineralized >98% of 100 mg.L-1 of Lindane after 26 h of cultivation without any accumulation of toxic metabolite(s) in the reaction medium, demonstrating the efficiency of the biological system. Integrating both chemical and biological systems could provide significant advantages for the treatment of lindane, reducing the treatment time and overall cost. This synergistic approach can significantly enhance the overall removal efficiency of lindane from contaminated soil and water.

The current study is about detoxifying soil and water contaminated with toxic Cr(VI). To ensure that DAS1 could develop as well as possible, the pH was changed between 4 and 10. DAS1 showed its highest growth at pH 8, and at the same pH, it had an 85% potential to remediate by converting Cr(VI) to Cr(III). Immobilized bacteria increased the reduction of Cr(VI) to Cr(III) from the culture medium to 90.4%. The impact of glucose concentrations between 0.5 and 2.5 g.L-1 was examined. The greatest development was seen at pH 8 and 2 g.L-1 glucose concentration. The remediation potential was improved by up to 96% when the growing medium contained 200 mg.L-1 Cr(VI). The value of ks (0.434 g.L-1) demonstrated the substrate’s affinity for bacteria in accordance with the Monod equation, while μ max (0.090 h) demonstrated that DAS1 required 11.11 h for maximal growth. The multifactor experimental design was used to analyze mixed cultures of DAS1 and DAS2 in a 1:1 ratio, and it was determined that the X3Y2Z1 experiment design was best for completely removing Cr(VI) from the growing medium. By making pores using Na2EDTA, it was determined that the cell membrane’s impermeability did not cause Cr(VI) resistance in DAS1. The delayed lag phase indicated that the enzyme activity was inductive rather than constitutive.