With the upgrade of wastewater treatment plants (WWTPs) to meet more stringent discharge limits for nutrients, dissolved organic nitrogen (DON) is present at an increasing percentage (up to 85%) in the effluent. Discharged DON is of great environmental concern due to its potentials in stimulating algal growth and forming toxic nitrogenous disinfection by-products (N-DBPs). This article systematically reviewed the characteristics, transformation and ecological impacts of wastewater DON. Proteins, amino acids and humic substances are the abundant DON compounds, but a large fraction (nearly 50%) of DON remains uncharacterized. Biological treatment processes play a dominant role in DON transformation (65–90%), where DON serves as both nutrient and energy sources. Despite of the above progress, critical knowledge gaps remain in DON functional duality, relationship with dissolved inorganic nitrogen (DIN) species, and coupling/decoupling with the dissolved organic carbon (DOC) pool. Development of more rapid and accurate quantification methods, modeling transformation processes, and assessing DON-associated eutrophication and N-DBP formation risks should be given priority in further investigations.

To constrain sources of anthropogenic nitrogen (N) deposition is critical for effective reduction of reactive N emissions and better evaluation of N deposition effects. This study measured δ¹⁵N signatures of nitrate (NO3⁻), ammonium (NH4⁺) and total dissolved N (TDN) in precipitation at Guiyang, southwestern China and estimated contributions of dominant N sources using a Bayesian isotope mixing model. For NO3⁻, the contribution of non-fossil N oxides (NOx, mainly from biomass burning (24 ± 12%) and microbial N cycle (26 ± 5%)) equals that of fossil NOx, to which vehicle exhausts (31 ± 19%) contributed more than coal combustion (19 ± 9%). For NH4⁺, ammonia (NH3) from volatilization sources (mainly animal wastes (22 ± 12%) and fertilizers (22 ± 10%)) contributed less than NH3 from combustion sources (mainly biomass burning (17 ± 8%), vehicle exhausts (19 ± 11%) and coal combustions (19 ± 12%)). Dissolved organic N (DON) accounted for 41% in precipitation TDN deposition during the study period. Precipitation DON had higher δ¹⁵N values in cooler months (13.1‰) than in warmer months (−7.0‰), indicating the dominance of primary and secondary ON sources, respectively. These results newly underscored the importance of non-fossil NOx, fossil NH3 and organic N in precipitation N inputs of urban environments.

Long term trend analysis of bulk precipitation, throughfall and soil solution elemental fluxes from 12 years monitoring at 10 ICP Level II forest sites in the UK reveal coherent national chemical trends indicating recovery from sulphur deposition and acidification. Soil solution pH increased and sulphate and aluminium decreased at most sites. Trends in nitrogen were variable and dependant on its form. Dissolved organic nitrogen increased in bulk precipitation, throughfall and soil solution at most sites. Nitrate in soil solution declined at sites receiving high nitrogen deposition. Increase in soil dissolved organic carbon was detected - a response to pollution recovery, changes in soil temperature and/or increased microbial activity. An increase of sodium and chloride was evident - a possible result of more frequent storm events at exposed sites. The intensive and integrated nature of monitoring enables the relationships between climate/pollutant exposure and chemical/biological response in forestry to be explored.

This study investigated the seasonal characterization of dissolved organic matter (DOM) in reclaimed wastewater (RW) with a special focus on dissolved organic nitrogen (DON) from two full-scale municipal wastewater reclamation plants (WRPs) where the produced RW was used to augment urban rivers. Results showed that the concentrations of DON in RW ranged from 0.32 mg/L to 1.21 mg/L. A higher seasonal mean value of DON in RW from both of the WRPs was observed in winter (p < 0.05, ANOVA). DON chemical characteristics analysis, including ultrahigh-resolution mass spectrometry and ultrafiltration fractionation, showed that DON in RW exhibits more lability during winter than during the other three seasons. This finding was also supported by the results of an algal bioassay experiment, in which DON bioavailabilities were 63.7 ± 3.0%, 53.0 ± 5.3%, 49.5 ± 0.5%, and 49.8 ± 0.2% for WRP-A and were 60.8 ± 2.4%, 43.7 ± 2.2%, 41.2 ± 1.7%, and 43.1 ± 1.1% for WRP-B in winter, spring, summer, and autumn, respectively. Accordingly, DON in RW during winter is more prone to stimulate natural algae and microorganisms, which gives rise to eutrophication in urban rivers. At the molecular level, the seasonal changes in DON are not coupled with those of DOC, which highlights the necessity of DON measurement to obtain a comprehensive understanding of the seasonal characteristics of DOM in RW and its effect on wastewater reuse in urban rivers.

Vegetable production in solar greenhouses in northern China results in the excessive use of nitrogen (N) fertilizers and water via flooding irrigation. Both factors result in low N use efficiency and high environmental costs because groundwater becomes contaminated with nitrate (NO3−). Four consecutive tomato (Lycopersicum esculentum Mill.) cropping seasons were tested whether drip fertigation and/or the incorporation of maize straw (S) may significantly reduce NO3− and dissolved organic N (DON) leaching while increasing the water-use efficiency (WUE) and partial factor productivity of applied N (PFPN) of the tomatoes. The following treatments were used: ① conventional flooding irrigation with overfertilization (CIF, 900 kg N ha−1 season−1), ② CIF + S, ③ drip irrigation with optimized fertilization (DIF, 400 kg N ha−1 season−1), ④ DIF + S. We found that (1) DIF significantly increases the PFPN and WUE by 262% and 73% without compromising the yield compared with CIF, respectively. (2) For CIF, approximately 50% of the total N input was leached at a NO3−/DON ratio of approximately 2:1. (3) Compared with CIF, DIF reduced NO3− and DON leaching by 88% and 90%, respectively. Water percolation was positively correlated with N leaching (p < 0.001). (4) Straw application only reduced NO3− leaching losses in the first year and did not affect DON leaching overall, although DON leaching was increased in DIF in the first growing season. In conclusion, DIF significantly reduces NO3− and DON leaching losses by approximately 90% compared with the current farmer practice (CIF). Considering the significant DON leaching losses, which have been overlooked because previous measurements focused on NO3−, DON should be considered as a primary factor of environmental pollution in conventional solar greenhouse vegetable production systems.

Six different approaches are applied in the present study to apportion the sources of precipitation nitrogen making use of precipitation data of dissolved inorganic nitrogen (DIN, including NO3− and NH4+), dissolved organic nitrogen (DON) and δ15N signatures of DIN collected at six sampling sites in the mountain region of Southwest China. These approaches include one quantitative approach running a Bayesian isotope mixing model (SIAR model) and five qualitative approaches based on in-situ survey (ISS), ratio of NH4+/NO3− (RN), principal component analysis (PCA), canonical-correlation analysis (CCA) and stable isotope approach (SIA). Biomass burning, coal combustion and mobile exhausts in the mountain region are identified as major sources for precipitation DIN while biomass burning and volatilization sources such as animal husbandries are major ones for DON. SIAR model results suggest that mobile exhausts, biomass burning and coal combustion contributed 25.1 ± 14.0%, 26.0 ± 14.1% and 27.0 ± 12.6%, respectively, to NO3− on the regional scale. Higher contributions of both biomass burning and coal combustion appeared at rural and urban sites with a significant difference between Houba (rural) and the wetland site (p < 0.05). The RN method fails to properly identify sources of DIN, the ISS and SIA approach only respectively identifies DON and DIN sources, the PCA only tracks source types for precipitation N, while the CCA identify sources of both DIN and DON in precipitation. SIAR quantified the contributions of major sources to precipitation NO3− but failed for precipitation NH4+ and DON. It is recommended to use ISS and SIAR in combination with one or more approaches from PCA, CCA and SIA to apportion precipitation NO3− sources. As for apportioning precipitation NH4+ sources, more knowledge is needed for local 15N databases of NH3 and DON and 15N fractional mechanisms among gaseous, liquid and particulate surfaces in this mountain region and similar environments.

The quantification of atmospheric deposition flux is essential for assessment of its impact on ecosystems. We present an advanced approach for the estimation of the spatial pattern of atmospheric nitrogen deposition flux over the Czech forests, collating all available measured data and model results. The aim of the presented study is to provide an improved, more complete, more reliable and more realistic estimate of the spatial pattern of nitrogen deposition flux over one country. This has so far usually been based on measurements of ambient NOx concentrations as dry deposition proxy, and NH4+ and NO3− in precipitation as wet deposition proxy. For estimation of unmeasured species contributing to dry deposition, we used the CAMx Eulerian photochemical dispersion model, coupled with the Aladin regional numeric weather prediction model. The contribution of fog and dissolved organic nitrogen was estimated using a geostatistical data driven model. We prepared individual maps for particular components applying the most relevant approach and then merged all layers to obtain a final map representing the best estimate of nitrogen deposition over the Czech Republic. Final maps accounting for unmeasured species clearly indicate that the approach used so far may result in a substantial underestimation of nitrogen deposition flux. Our results showed that nitrogen deposition over the Czech forested area in 2008 was well above 2 g N m−2 yr−1, with almost 70% of forested area receiving 3–4 g N m−2 yr−1. NH3 and gaseous HNO3, contributing about 80%, dominated the dry nitrogen deposition. Estimating the unmeasured nitrogen species by modeled values provides realistic approximations of total nitrogen deposition that also result in more realistic spatial patterns that could be used as input for further studies of likely nitrogen impacts on ecosystems.

Syracuse, New York is working under a court-ordered agreement to limit combined sewer overflows (CSO) to local surface waters. Green infrastructure technologies, including green roofs, are being implemented as part of a CSO abatement strategy and to develop co-benefits of diminished stormwater runoff, including decreased loading of contaminants to the wastewater system and surface waters. The objective of this study was to examine the quantity and quality of discharge associated with precipitation events over an annual cycle from a green roof in Syracuse, NY and to compare measurements from this monitoring program with results from a roof irrigation experiment. Wet deposition, roof drainage, and water quality were measured for 87 storm events during an approximately 12 month period over 2011–2012. Water and nutrient (total phosphorus, total nitrogen, and dissolved organic carbon) mass balances were conducted on an event basis to evaluate retention annually and during the growing and non-growing seasons. These results are compared with a hydrological manipulation experiment, which comprised of artificially watering of the roof. Loadings of nutrients were calculated for experimental and actual storms using the concentration of nutrients and the flow data of water discharging the roof. The green roof was effective in retaining precipitation quantity from storm events (mean percent retention 96.8%, SD = 2.7%, n = 87), although the relative fraction of water retained decreased with increases in the size of the event. There was no difference in water retention of the green roof for the growing and non-growing seasons. Drainage waters exhibited high concentration of nutrients during the warm temperature growing season, particularly total nitrogen and dissolved organic carbon. Overall, nutrient losses were low because of the strong retention of water. However, there was marked variation in the retention of nutrients by season due to variations in concentrations in roof runoff.

This work aimed to study the performance of paraquat removal by cell-immobilized ceramics. Two strains of paraquat degrading bacteria, Pseudomonas putida and Bacillus subtilis, were separately immobilized on the ceramic with and without wastewater sludge addition. Results showed that the ceramic surface with sludge has more functional groups and a more highly negative charge on the surface than the original ceramic. The ceramic with sludge had 2-3-fold of the immobilized cells higher than that of the control (without sludge) and less leaching of the immobilized cells. The sludge addition at 20% (w/w) to the ceramic provided the highest cell adhesion for both P. putida and B. subtilis. The paraquat removal efficiencies were higher than 98%, while the control ceramic could remove only 77 ± 1.2%. The immobilized cells on ceramic with sludge provided a significant degree of dissolved organic nitrogen reduction (82%) during the paraquat removal. Most organic nitrogen in paraquat was biologically mineralized (ammonified). Findings from this work suggest the superiority of ceramic with sludge in mineralizing organic nitrogen associated with paraquat.

Soil nitrogen (N) leaching is recognized to have negative effects on the environment. There is a lack of studies on different simultaneously occurring drivers of environmental change, including changing rainfall and N deposition, on soil N leaching. In this study, a two factorial field experiment was conducted in a Korean pine forest with the following four treatments: 30% of throughfall reduction (TR), 50 kg N ha⁻¹ yr⁻¹ of N addition (N+), throughfall reduction plus N addition (TRN+) and natural forest (CK). The zero-tension pan lysimeter method was used to assess the response of soil N leaching loss to manipulated N addition and throughfall reduction. The results showed that the soil N leaching loss in natural forest was 5.0 ± 0.4 kg N ha⁻¹yr⁻¹, of which dissolved organic nitrogen (DON) accounted for 48%. Compared to natural forest, six years of N addition (NH₄NO₃, 50 kg N ha⁻¹ year⁻¹) significantly (P < 0.05) increased soil N leaching losses by 122%, especially in the form of NO₃⁻; a 30% reduction in throughfall slightly decreased N leaching losses by 23%; in combination, N addition and throughfall reduction increased N leaching losses by 48%. There was a strong interaction between N addition and throughfall reduction, which decreased N leaching loss by approximately 2.5 kg N ha⁻¹ yr⁻¹. Our results indicated that drought would diminish the enhancing effect of N deposition on soil N leaching. These findings highlight the importance of incorporating both N deposition and precipitation and their impacts on soil N leaching into future N budget assessments of forest ecosystems under global environmental change.