Arsenic is inorganic spread out in nature. Toxicity of arsenic is different, depending on its form. However, current international and Serbian legislation in drinking water regulate maximum allowed concentration of 0.010 mg/l (of total arsenic), regardless of its form. Concentration of total arsenic in water of springs of mountain Avala (Serbia) is higher than 0.010 mg/l. This paper present determination of inorganic arsenic in drinking water of spring Sakinac (Avala), also determination which form is more represented, relation of As(III) and As(V) and implementation of legislation. We used stripping voltametry method and gain value of total arsenic-62.87 microgram/l, in which 3.97 microgram/l is As(III) and residue of 58.90 microgram/l is As(V). It is obvious that value of 62.87 microgram/l is higher than MAL, but the most abundant arsenic form is less toxic, so the question is whether the water from spring Sakinac can or cannot be used.

Arsenic speciation can be very important in analysis of water, foodstuffs, and environmental samples, besides of total arsenic analysis. Hydride generation atomic absorption spectrometry can be used for speciation analysis based on different hydride generation condition. In our work, we studied effect of L-ascorbic acid like the reaction medium and the prereducing agent.