Because of environmental and societal concerns, new strategies are being developed to mitigate the effects of road salt. These include new deicers that are alternatives to or mixtures with the most common road salt, sodium chloride (NaCl), improved techniques and equipment, and biotic mitigation methods. Using outdoor mesocosms, we investigated the impacts of NaCl and two common alternatives, magnesium chloride (MgCl₂) and calcium chloride (CaCl₂) on freshwater communities. We also investigated the mitigation ability of a common macrophyte, Elodea. We hypothesized that road salt exposure reduces filamentous algae, zooplankton, and macrocrustaceans, but results in increases in phytoplankton and gastropods. We also hypothesized that MgCl₂ is the most toxic salt to communities, followed by CaCl₂, and then NaCl. Lastly, we hypothesized that macrophytes mitigate some of the effects of road salt, specifically the effects on primary producers. We found that all three salts reduced filamentous algal biomass and amphipod abundance, but only MgCl₂ reduced Elodea biomass. MgCl₂ had the largest and longest lasting effects on zooplankton, specifically cladocerans and copepods, which resulted in a significant increase in phytoplankton and rotifers. CaCl₂ increased ostracods and decreased snail abundance, but NaCl increased snail abundance. Lastly, while we did not find many interactions between road salt and macrophyte treatments, macrophytes did counteract many of the salt effects on producers, leading to decreased phytoplankton, increased filamentous algae, and altered abiotic responses. Thus, at similar chloride concentrations, NaCl alternatives, specifically MgCl₂, are not safer for aquatic ecosystems and more research is needed to find safer road management strategies to protect freshwater ecosystems.

The co-occurrence of aquatic contaminants, such as antibiotics and herbicides, has motivated investigations into their interactive effects on aquatic organisms. We examined the combined effects of environmental concentrations of the antibiotic Enrofloxacin (Enro; 0–2.25 μg l−1) and Roundup OriginalDI (Roundup®; 0–0.75 μg active ingredient l−1), a glyphosate based-herbicide, on Elodea canadensis. Enro alone was not toxic, but the plants were highly sensitive to Roundup® whose toxicity is related to the induction of oxidative stress. The metabolism of Enro by plants into Ciprofloxacin (Cipro) was observed, and although former is not phytotoxic, oxidative events associated with Cipro generation were observed. The activity of cytochrome P450 was shown to be involved in Enro degradation in E. canadensis. As a cytochrome P450 inhibitor, Roundup® decreases Enro metabolism in plants. Enro, in turn, increases glyphosate uptake and toxicity, so that Enro and Roundup® have synergistic effects, disrupting the physiological processes of E. canadensis. Our results suggest E. canadensis as a potential candidate for the reclamation of Enro in contaminated waters, but not for Roundup® due to its high sensitivity to that herbicide.

The submersed macrophytes Elodea canadensis, Myriophyllum spicatum and Potamogeton lucens were constantly exposed over a five-week period to environmentally relevant concentrations of atrazine, isoproturon, diuron, and their mixture in outdoor mesocosms. Effects were evaluated investigating photosynthetic efficiency (PE) of the three macrophytes and growth of M. spicatum and E. canadensis. Adverse effects on PE were observed on days 2 and 5 after application. M. spicatum was found to be the more sensitive macrophyte. E. canadensis and P. lucens were less sensitive to atrazine, diuron and the mixture and insensitive to isoproturon. PE of M. spicatum was similarly affected by the single herbicides and the mixture demonstrating concentration addition. Growth of E. canadensis and M. spicatum was not reduced indicating that herbicide exposure did not impair plant development. Although PE measurements turned out to be a sensitive method to monitor PSII herbicides, plant growth remains the more relevant ecological endpoint in risk assessment. Short-term effects on photosynthesis did not result in growth reduction of submerse macrophytes exposed to PSII inhibitors.

Understanding the processes that regulate contaminant impacts in nature is an increasingly important challenge. For insecticides in surface waters, the ability of aquatic plants to sorb, or bind, hydrophobic compounds has been identified as a primary mechanism by which toxicity can be mitigated (i.e. the sorption-based model). However, recent research shows that submerged plants can also rapidly mitigate the toxicity of the less hydrophobic insecticide malathion via alkaline hydrolysis (i.e. the hydrolysis-based model) driven by increased water pH resulting from photosynthesis. However, it is still unknown how generalizable these mitigation mechanisms are across the wide variety of insecticides applied today, and whether any general rules can be ascertained about which types of chemicals may be mitigated by each mechanism. We quantified the degree to which the submerged plant Elodea canadensis mitigated acute (48-h) toxicity to Daphnia magna using nine commonly applied insecticides spanning three chemical classes (carbamates: aldicarb, carbaryl, carbofuran; organophosphates: malathion, diazinon, chlorpyrifos; pyrethroids: permethrin, bifenthrin, lambda-cyhalothrin). We found that insecticides possessing either high octanol-water partition coefficients (log Kow) values (i.e. pyrethroids) or high susceptibility to alkaline hydrolysis (i.e. carbamates and malathion) were all mitigated to some degree by E. canadensis, while the plant had no effect on insecticides possessing intermediate log Kow values and low susceptibility to hydrolysis (i.e. chlorpyrifos and diazinon). Our results provide the first general insights into which types of insecticides are likely to be mitigated by different mechanisms based on known chemical properties. We suggest that current models and mitigation strategies would be improved by the consideration of both mitigation models.

Nanotechnology is a dynamically developing field of scientific and industrial interest across the entire world, and the commercialization of nanoparticles (NPs) is rapidly expanding. Incorporation of nanotechnologies into a range of manufactured goods results in increasing concern regarding the subsequent release of engineered NPs into the environment. One of the biggest threats of using NPs is the transfer and magnification of these particles in the trophic chain. The aim of the studies was the evaluation of the distribution of TiO₂ NP contamination in the aquatic ecosystem under laboratory conditions. Bioaccumulation of TiO₂ NPs by plants (Elodea canadensis) and fish (Danio rerio) in the source of contamination was investigated. The studies were focused on the consequences of short-term water contamination with TiO₂ NPs and the secondary contamination of the components of the investigated model ecosystem (plants, sediments). It was found that in the fish and the plants exposed to NP contamination, the amount of Ti was higher than in the control, indicating an effective bioaccumulation of NPs or ions originating from NPs. It was clearly shown that the NPs present in the sediments are available to plants and fish. Additionally, the aquatic plants, an important trophic level in the food chain, can accumulate NPs and be a source of NPs for higher organisms. It was concluded that even an incidental contamination of water by NPs may result in long-term consequences induced by the release of NPs.

Increasing environmental concentrations of platinum group metals (PGMs), in particular platinum (Pt), rhodium (Rh) and palladium (Pd), from catalytic converters has been reported worldwide. The impact of these three metals on the uptake and use of essential mineral nutrients was examined using two plant models: the submerged aquatic plant, Elodea canadensis, and the terrestrial emergent plant, Peltandra virginica. Plants were grown for 2 weeks in nutrient solutions with either Pt⁴⁺ at concentrations between 0.05 and 5 mg/L, or a 0.1 mg/L Pt⁴⁺, Rh³⁺, Pd²⁺ mixture. Some treatments received additional Ca²⁺, Zn²⁺, or humic acid (with varying pH) to study how these conditions affected PGM uptake. Metal concentration analyses were conducted using a graphite furnace atomic absorption spectrometer (GFAAS) or an inductively coupled plasma emission spectrometer (ICP). Growth response was assessed through total chlorophyll content. There was significant Pt accumulation in plant tissues, from 55 to 326 times the concentration in nutrient solution. At pH 8, the addition of humic acid doubled Pt accumulation in comparison to the control. Additional exogenous minerals did not significantly affect PGM uptake, nor did the uptake of PGMs interfere with the uptake of Ca, Fe or Cu. Synthesis of chlorophyll in new shoots was not affected by Pt accumulation; however, visible chlorosis was observed in older shoots at 5 ppm Pt. Roadside Daucus carota samples from four heavy traffic locations in Dutchess County (New York) were also assessed for PGM content. Pt, Pd and Rh concentrations averaged 14.6, 10.2, and 0.7 μg/g, respectively.

In this study, the potential association of (citrate-stabilized) Ag (14.1 ± 1.0 nm) and CeO₂ (6.7 ± 1.2 nm) engineered nanoparticles (ENPs), or their ionic counterparts, with the submerged aquatic plant Elodea canadensis, was examined and, in particular, parameters affecting the distribution of the nanoparticles (or metal ions) between plant biomass and the water phase were assessed using five distinct aqueous matrices (i.e. tap water, 10 % Hoagland’s solution and three natural surface water samples). Individual plants were exposed to varying concentrations of Ag and CeO₂ ENPs or Ag⁺ and Ce³⁺ ions during 72-h-lasting batch experiments. A dose-dependent increase of silver or cerium in plant biomass was observed for both the nanoparticles and the ions, whereby exposure to the latter systematically resulted in significantly higher biomass concentrations. Furthermore, the apparent plant uptake of CeO₂ ENPs appeared to be higher than that for Ag ENPs when comparing similar exposure concentrations. These findings suggest that association with E. canadensis might be affected by particle characteristics such as size, composition, surface charge or surface coating. Moreover, the stability of the ENPs or ions in suspension/solution may be another important aspect affecting plant exposure and uptake. The association of the nanoparticles or ions with E. canadensis was affected by the physicochemical characteristics of the water sample. The silver biomass concentration was found to correlate significantly with the electrical conductivity (EC), dry residue (DR) and Cl⁻, K, Na and Mg content in the case of Ag ENPs or with the EC, inorganic carbon (IC) and Cl⁻, NO₃⁻, Na and Mg content in the case of Ag⁺ ions, whereas significant relationships between the cerium biomass concentration and the EC, DR, IC and Ca content or the pH, EC, DR, IC and Cl⁻, Ca and Mg content were obtained for CeO₂ ENPs or Ce³⁺ ions, respectively. Results also indicated that the Ag ENPs and Ag⁺ ions might potentially be toxic towards E. canadensis whereas no evidence of phytotoxicity was noted in the case of CeO₂ ENPs or Ce³⁺ ions.

Model aquatic ecosystems have been used to study the natural mechanisms involved in the distribution and transformation of inorganic mercury (IHg) in the different compartments and its interactions with the biota. Laboratory incubations in indoor freshwater microcosms, presenting a simple biological organization, were carried out at various spiked concentrations (3, 25 and 257 nmol l-¹ of IHg, as mercuric chloride) and from a single initial contamination of the water column. The different compartments of the model ecosystems (water, sediment, macrophytes Elodea canadensis and snails Lymnaea stagnalis) were investigated for mercury distribution and speciation during a 2-month experimental period. The principal results obtained have evidenced different Hg biogeochemical pathways including biotic IHg methylation and reduction and transfer to the biota. A fast transfer of IHg from the water to the aquatic organisms and to the sediment was first observed with IHg half-lives of 24 h and 8 days, respectively. IHg methylation, clearly related to biogenic processes, was also demonstrated in all contaminated microcosms after 1 week of exposure. Finally, gaseous mercury species were determined in the different microcosms and significant biological induced production of elemental Hg (Hg°) and dimethyl Hg (DMHg) was observed. This overall investigation, based on the time courses evolution of IHg and in situ produced monomethylmercury (MMHg) concentrations allows to determine uptake and elimination rate constants for IHg as well as the bioaccumulation kinetics of MMHg in macrophytes and snails. The applicability of these aquatic model ecosystems to provide real insights for pollution impact and ecotoxicological risk assessments has been demonstrated.