Several studies have explored the utilization of soil microorganisms, to address the environmental issues associated with glyphosate use and enhance crop yields. In our investigation, screening on Agar plate and broth medium  Luria Bertani was carried out after isolating bacterial strains from rhizospheric agricultural soil in Mascara,  Algeria, to biodegrade glyphosate, following that by testing the Plant Growth-Promoting Rhizobacteria and evaluate the effects of glyphosate on these proprieties. Our findings indicate that five bacterial strains exhibited growth in the presence of glyphosate concentrations up to 25 mg/ml, beyond this concentration the strains have developed tolerance. Following a partial examination of the 16S rRNA sequences, the bacterial strains were identified as belonging to the genus of Enterobacter. After 10 days of incubation with the glyphosate, Phosphate solubilization decreased in broth and agar Pikovskaya medium and the bacterial strains synthetized less of indole-3-acetic acid compared to the control, indicating the impact of glyphosate on these outcomes, high concentration of glyphosate inhibited nitrogen fixation, and various doses of glyphosate were found to restrict the growth of biofilms in these strains. The results of HPLC examination of secondary metabolites revealed that the primary degradation products of glyphosate in all strains were Sarcosine and Glycine. So, it seemed that the strain could both biodegrade glyphosate and use it for growth ,while also possessing rhizobacteria properties that promote plant development, enabling the use of the strains in the bioremediation of glyphosate-contaminated soils.

Petroleum-polluted soil samples from Ahvaz oilfield were enriched, using three methods to detect microorganisms with different dibenzothiophene degradation capabilities. Strain NISOC-03, a nitrate-reducing, oxidase negative, catalase, citrate, and urease positive, gram negative rod, showed interesting dibenzothiophene desulfurization behavior, designated as Entreobacter sp. strain NISOC-03 based on phenotype and genotype analyses. Gas chromatography, biomass measurement, and Gibb’s assay showed that in the presence of benzoate as the carbon source, strain NISOC-03 utilized 64% of 0.8 mM dibenzothiophene, producing 0.27 mM phenyl phenol during the exponential growth phase, though the produced phenyl phenol was degraded in the stationary growth phase. In the presence of glucose as the carbon source, however, strain NISOC-03 metabolized only 19.6% of 0.8 mM dibenzothiophene. Furthermore, replacing glucose with ethanol or glycerol led to the same reduction of the dibenzothiophene utilization. It is thus concluded that the chemistry of the potential carbon source(s) in the culture medium has a significant influence on the quality and the rate of dibenzothiophene metablization, and the enrichment designation has a very vital effect on the biodegradation efficiency of the isolated microorganisms.

Persistent pollution of surface waters by hydrocarbon compounds is one of the foremost threats to limited global freshwater resources. This study analyzed the abundance, diversity and degradative capacities of hydrocarbon-utilizing bacteria in chronically polluted Kono River in the Nigerian Niger Delta in order to establish the bacterial drivers of ecological regeneration of the river after an oil spill. The study further aimed to develop a specialized bacterial consortium for application in bioremediation interventions. Bacillus, Pseudomonas and Enterobacter spp. were predominant out of the 82 isolates obtained. Klebsiella pneumoniae and two species of Enterobacter cloacae were identified as the most efficient hydrocarbon utilizers. The isolates were also confirmed as biosurfactant producers and possessed the alkB1 and nahAc genes for degradation of aliphatics and aromatics. E. cloacae-K11, K. pneumoniae-K05, E. cloacae-K12 and their consortium were able to degrade the total petroleum hydrocarbons and polycyclic aromatic hydrocarbons in batch systems by 59.37% – 96.06% and 68.40% – 92.46% respectively. K. pneumoniae-K05 showed the greatest petroleum degradation capacity of the three isolates but hydrocarbon degradation was most efficient with the bacterial consortium. The results obtained showed no significant differences at p≤0.05 between the degradation capacities of K. pneumoniae-K05 and the consortium for PAHs but a significant difference (p≤0.05) was seen with TPH degradation. A viable hydrocarbon degrading bacterial consortium was developed at the end of the study and it was concluded that the polluted river water displayed inherent potential for effective natural attenuation.