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Continuous low-level dietary exposure to glyphosate elicits dose and sex-dependent synaptic and microglial adaptations in the rodent brain.
2024
Cresto, Noemie | Courret, Margot | Génin, Athénaïs | Pauline Martin, Céline Marie | Bourret, Julie | Sakkaki, Sophie | de Bock, Frederic | Janvier, Alicia | Polizzi, Arnaud | Payrastre, Laurence | Ellero-Simatos, Sandrine | Audinat, Etienne | Perroy, Julie | Marchi, Nicola | Institut de Génomique Fonctionnelle (IGF) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) | ToxAlim (ToxAlim) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | IRCER - Axe 1 : procédés céramiques (IRCER-AXE1) ; Institut de Recherche sur les CERamiques (IRCER) ; Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut Matériaux Procédés Environnement Ouvrages (IMPEO) ; Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut Matériaux Procédés Environnement Ouvrages (IMPEO) ; Université de Limoges (UNILIM)-Université de Limoges (UNILIM) | Toxicologie Intégrative & Métabolisme (ToxAlim-TIM) ; ToxAlim (ToxAlim) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANR-19-CE34-0007,GLYFLORE,Impact of a chronic dietary exposure to low-doses of glyphosate on the gut microbiota and microbiota-associated physiological functions (metabolism, intestinal immunity, brain structures and behavior)(2019) | ANR-17-CE34-0005,HepatoBrain,Mélange de pesticides et axe foie-cerveau : implication des récepteurs aux xénobiotiques(2017) | ANR-22-CE17-0061,CEST-Focus,Biomarqueurs de type CEST pour délimiter la zone épileptique(2022) | ANR-19-CE16-0018,MicroSENSO,Les microglies dans l'assemblage des circuits inhibiteurs pendant les périodes critiques sensorielles(2019) | ANR-21-CE17-0031,EPI-CATCHER,Nouvelles modalités d'imagerie et de spectroscopie GABA pour localiser la zone épileptique(2021)
International audience | Prolonged exposure to low levels of dietary contaminants is a context in modern life that could alter organ physiology gradually. Here, we aimed to investigate the impact of continuous exposure to acceptable daily intake (ADI) and non-observable adverse effect level (NOAEL) of glyphosate from gestation to adulthood using C57BL/6J mice and incorporating these levels into their food pellets. From adulthood, we analyzed neurophysiological and neuro-glia cellular adaptations in male and female animals. Using ex-vivo hippocampal slice electrophysiology, we found a reduced efficacy of Schaffer collateral-to-CA1 excitatory synapses in glyphosate-exposed dietary conditions, with ADI and NOAEL dose-dependent effects. Short-term facilitation of excitatory synaptic transmission was specifically increased in NOAEL conditions, with a predominant influence in males, suggesting a reduced probability of neurotransmitter release. Long-term synaptic potentiation (LTP) was decreased in NOAEL-exposed mice. Next, we explore whether these neurophysiological modifications are associated with neuro-glia changes in the somatosensory cortex and hippocampus. High-resolution confocal microscopy analyses unveil a dose-dependent increased density of excitatory Vglut1+ Homer1+ synapses. Microglial Iba1+ cells displayed a shortening of their ramifications, a sign of cellular reactivity that was more pronounced in males at NOAEL levels. The morphology of GFAP+ astrocytes was generally not modified. Finally, we asked whether mouse-specific cross-correlations exist among all data sets generated. This examination included the novel object recognition (NOR) test performed before ex vivo functional and immunohistochemical examinations. We report a negative linear regression between the number of synapses and NOR or LTP maintenance when plotting ADI and NOAEL datasets. These results outline synaptic and microglial cell adaptations resulting from prenatal and continuous dietary low levels of glyphosate, discernible in, but not limited to, adult males exposed to the NOAEL. We discuss the significance of these findings to real-world consumer situations and long-term brain resilience.
Show more [+] Less [-]An Overview of the Need for Circular Economy on Electric Vehicle Batteries
2024
S. Padmanabhan, C. Joel, S. Mahalingam, J. R. Deepak, T. Vinod Kumar and Deborah Raj
Batteries are a widely utilized and simple method for powering electronic devices, particularly given the prevalence of individuals traveling to all gadgets. The escalating adoption of electric vehicles and portable electronic devices has led to a surge in the demand for lithium-ion batteries. Consequently, this has given rise to supply uncertainties in acquiring essential minerals such as lithium and cobalt, along with concerns about the proper disposal of dead batteries. The existing methods for battery recycling exhibit variations based on the individual chemistries of the batteries, hence influencing both cost factors and greenhouse gas emissions. Simultaneously, there exists a possibility for repurposing depleted batteries for low-tier energy storage applications. The absence of legislation pertaining to the secure storage and handling of waste streams contributes to the accumulation of refuse in exposed environments and the release of hazardous substances from landfills. In addition, contemporary battery manufacturing methods necessitate the utilization of innovative substances, such as ionic liquids for electrolytes and nanostructures for cathodes, to enhance the energy characteristics and longevity of batteries. The presence of uncertainties regarding the accurate assessment of the environmental consequences associated with novel battery chemicals has the potential to impede efforts aimed at recycling and containment. The objective of this analysis is to consolidate the existing knowledge regarding battery pollutants, both those that are recognized and those that remain uncertain, and to assess their potential environmental impacts. Additionally, this research aims to examine the current strategies and methods employed for the recycling of batteries in the circular economy.
Show more [+] Less [-]An Overview of the Need for Circular Economy on Electric Vehicle Batteries
2024
S. Padmanabhan, C. Joel, S. Mahalingam, J. R. Deepak, T. Vinod Kumar and Deborah Raj
Batteries are a widely utilized and simple method for powering electronic devices, particularly given the prevalence of individuals traveling to all gadgets. The escalating adoption of electric vehicles and portable electronic devices has led to a surge in the demand for lithium-ion batteries. Consequently, this has given rise to supply uncertainties in acquiring essential minerals such as lithium and cobalt, along with concerns about the proper disposal of dead batteries. The existing methods for battery recycling exhibit variations based on the individual chemistries of the batteries, hence influencing both cost factors and greenhouse gas emissions. Simultaneously, there exists a possibility for repurposing depleted batteries for low-tier energy storage applications. The absence of legislation pertaining to the secure storage and handling of waste streams contributes to the accumulation of refuse in exposed environments and the release of hazardous substances from landfills. In addition, contemporary battery manufacturing methods necessitate the utilization of innovative substances, such as ionic liquids for electrolytes and nanostructures for cathodes, to enhance the energy characteristics and longevity of batteries. The presence of uncertainties regarding the accurate assessment of the environmental consequences associated with novel battery chemicals has the potential to impede efforts aimed at recycling and containment. The objective of this analysis is to consolidate the existing knowledge regarding battery pollutants, both those that are recognized and those that remain uncertain, and to assess their potential environmental impacts. Additionally, this research aims to examine the current strategies and methods employed for the recycling of batteries in the circular economy.
Show more [+] Less [-]