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A workflow for improving estimates of microplastic contamination in marine waters: A case study from North-Western Australia
2018
Kroon, Frederieke | Motti, Cherie | Talbot, Sam | Sobral, Paula | Puotinen, Marji
Plastic pollution is ubiquitous throughout the marine environment, with microplastic (i.e. <5 mm) contamination a global issue of emerging concern. The lack of universally accepted methods for quantifying microplastic contamination, including consistent application of microscopy, photography, an spectroscopy and photography, may result in unrealistic contamination estimates. Here, we present and apply an analysis workflow tailored to quantifying microplastic contamination in marine waters, incorporating stereomicroscopic visual sorting, microscopic photography and attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy. The workflow outlines step-by-step processing and associated decision making, thereby reducing bias in plastic identification and improving confidence in contamination estimates. Specific processing steps include (i) the use of a commercial algorithm-based comparison of particle spectra against an extensive commercially curated spectral library, followed by spectral interpretation to establish the chemical composition, (ii) a comparison against a customised contaminant spectral library to eliminate procedural contaminants, and (iii) final assignment of particles as either natural- or anthropogenic-derived materials, based on chemical type, a compare analysis of each particle against other particle spectra, and physical characteristics of particles. Applying this workflow to 54 tow samples collected in marine waters of North-Western Australia visually identified 248 potential anthropogenic particles. Subsequent ATR-FTIR spectroscopy, chemical assignment and visual re-inspection of photographs established 144 (58%) particles to be of anthropogenic origin. Of the original 248 particles, 97 (39%) were ultimately confirmed to be plastics, with 85 of these (34%) classified as microplastics, demonstrating that over 60% of particles may be misidentified as plastics if visual identification is not complemented by spectroscopy. Combined, this tailored analysis workflow outlines a consistent and sequential process to quantify contamination by microplastics and other anthropogenic microparticles in marine waters. Importantly, its application will contribute to more realistic estimates of microplastic contamination in marine waters, informing both ecological risk assessments and experimental concentrations in effect studies.
Show more [+] Less [-]Risk assessment of microplastics in the ocean: Modelling approach and first conclusions
2018
Everaert, Gert | Van Cauwenberghe, Lisbeth | De Rijcke, Maarten | Koelmans, Albert A. | Mees, Jan | Vandegehuchte, Michiel | Janssen, Colin R.
We performed an environmental risk assessment for microplastics (<5 mm) in the marine environment by estimating the order of magnitude of the past, present and future concentrations based on global plastic production data. In 2100, from 9.6 to 48.8 particles m⁻³ are predicted to float around in the ocean, which is a 50-fold increase compared to the present-day concentrations. From a meta-analysis with effect data available in literature, we derived a safe concentration of 6650 buoyant particles m⁻³ below which adverse effects are not likely to occur. Our risk assessment (excluding the potential role of microplastics as chemical vectors) suggests that on average, no direct effects of free-floating microplastics in the marine environment are to be expected up to the year 2100. Yet, even today, the safe concentration can be exceeded in sites that are heavily polluted with buoyant microplastics. In the marine benthic compartment between 32 and 144 particles kg⁻¹ dry sediment are predicted to be present in the beach deposition zone. Despite the scarcity of effect data, we expect adverse ecological effects along the coast as of the second half of the 21st century. From then ambient concentrations will start to outrange the safe concentration of sedimented microplastics (i.e. 540 particles kg⁻¹ sediment). Additional ecotoxicological research in which marine species are chronically exposed to realistic environmental microplastic concentration series are urgently needed to verify our findings.
Show more [+] Less [-]Environmental risk assessment of triclosan and ibuprofen in marine sediments using individual and sub-individual endpoints
2018
Pusceddu, F.H. | Choueri, R.B. | Pereira, C.D.S. | Cortez, F.S. | Santos, D.R.A. | Moreno, B.B. | Santos, A.R. | Rogero, J.R. | César, A.
The guidelines for the Environmental Risk Assessment (ERA) of pharmaceuticals and personal care products (PPCP) recommend the use of standard ecotoxicity assays and the assessment of endpoints at the individual level to evaluate potential effects of PPCP on biota. However, effects at the sub-individual level can also affect the ecological fitness of marine organisms chronically exposed to PPCP. The aim of the current study was to evaluate the environmental risk of two PPCP in marine sediments: triclosan (TCS) and ibuprofen (IBU), using sub-individual and developmental endpoints. The environmental levels of TCS and IBU were quantified in marine sediments from the vicinities of the Santos submarine sewage outfall (Santos Bay, São Paulo, Brazil) at 15.14 and 49.0 ng g⁻¹, respectively. A battery (n = 3) of chronic bioassays (embryo-larval development) with a sea urchin (Lytechinus variegatus) and a bivalve (Perna perna) were performed using two exposure conditions: sediment-water interface and elutriates. Moreover, physiological stress through the Neutral Red Retention Time Assay (NRRT) was assessed in the estuarine bivalve Mytella charruana exposed to TCS and IBU spiked sediments. These compounds affected the development of L. variegatus and P. perna (75 ng g⁻¹ for TCS and 15 ng g⁻¹ for IBU), and caused a significant decrease in M. charruana lysosomal membrane stability at environmentally relevant concentrations (0.08 ng g⁻¹ for TCS and 0.15 ng g⁻¹ for IBU). Chemical and ecotoxicological data were integrated and the risk quotient estimated for TCS and IBU were higher than 1.0, indicating a high environmental risk of these compounds in sediments. These are the first data of sediment risk assessment of pharmaceuticals and personal care products of Latin America. In addition, the results suggest that the ERA based only on individual-level and standard toxicity tests may overlook other biological effects that can affect the health of marine organisms exposed to PPCP.
Show more [+] Less [-]Assessing the influence of the genetically modified factor on mixture toxicological interactions in Caenorhabditis elegans: Comparison between wild type and a SOD type
2018
Li, Kai | Xu, Ya-Qian | Feng, Li | Liu, Shu-Shen
How to evaluate the ecological risk of transgenic technology is a focus of scientists because of the safety concerns raised by genetically modified (GM) organisms. Nevertheless, most studies are based on individual chemicals and always analyze the GM organism as a type of toxicant. In this study, we changed the approach and used GM organisms as the test objects with normal chemical exposure. Three types of chemicals (two substituted phenols, 4-chlorophenol and 4-nitrophenol; two ionic liquids, 1-butylpyridinium chloride and 1-butylpyridinium bromide; two pesticides, dichlorvos and glyphosate) were used to construct a six-component mixture system. The lethality to wild-type (N2) and sod-3::GFP (SOD-3) Caenorhabditis elegans was determined when they were exposed to the same mixture system after 12 and 24 h. The results showed that the pEC50 values of all of the single chemicals on SOD-3 were greater than those on N2 at 24 h. The toxicities of the single chemicals and nine mixture rays on the two strains increased with time. Notably, we discovered a significant difference between the two strains; time-dependent synergism occurred in mixtures on N2, but time-dependent antagonism occurred in mixtures on SOD-3. Finally, the strength of the synergism or antagonism turned to additive action on the two strains as the exposure time increased. These findings illustrated that the GM factor of the nematode influenced the mixture toxicological interaction at some exposure times. Compared with N2, SOD-3 were more sensitive to stress or toxic reactions. Therefore, the influence of the GM factor on mixture toxicological interactions in environmental risk assessment must be considered.
Show more [+] Less [-]Occurrence and ecological risk assessment of organic micropollutants in the lower reaches of the Yangtze River, China: A case study of water diversion
2018
Yan, Zhenhua | Yang, Haohan | Dong, Huike | Ma, Binni | Sun, Hongwei | Pan, Ting | Jiang, Runren | Zhou, Ranran | Shen, Jie | Liu, Jianchao | Lü, Guanghua
Water diversion has been increasingly applied to improve water quality in many water bodies. However, little is known regarding pollution by organic micropollutants (OMPs) in water diversion projects, especially at the supplier, and this pollution may threaten the quality of transferred water. In the present study, a total of 110 OMPs belonging to seven classes were investigated in water and sediment collected from a supplier of the Yangtze River within four water diversion projects. A total of 69 and 58 target OMPs were detected in water and sediment, respectively, at total concentrations reaching 1041.78 ng/L and 5942.24 ng/g dry weight (dw). Polycyclic aromatic hydrocarbons (PAHs) and pharmaceuticals were the predominant pollutants identified. When preliminarily compared with the pollution in the receiving water, the Yangtze River generally exhibited mild OMPs pollution and good water quality parameters, implying a clean water source in the water diversion project. However, in Zongyang and Fenghuangjing, PAHs pollution was more abundant than that in the corresponding receiving water in Chaohu Lake. Ammonia nitrogen pollution in the Wangyu River was comparable to that in Taihu Lake. These findings imply that water diversion may threaten receiving waters in some cases. In addition, the risks of all detected pollutants in both water and sediment were assessed. PAHs in water, especially phenanthrene and high-molecular-weight PAHs, posed high risks to invertebrates, followed by the risks to fish and algae. Pharmaceuticals, such as antibiotics and antidepressants, may also pose risks to algae and fish at a number of locations. To the best of our knowledge, this report is the first to describe OMPs pollution in water diversion projects, and the results provide a new perspective regarding the security of water diversion projects.
Show more [+] Less [-]Integrated regional ecological risk assessment of multiple metals in the soils: A case in the region around the Bohai Sea and the Yellow Sea
2018
Shi, Yajuan | Xu, Xiangbo | Li, Qifeng | Zhang, Meng | Li, Jing | Lü, Yonglong | Liang, Ruoyu | Zheng, Xiaoqi | Shao, Xiuqing
Methodology to quantify and distinguish the spatial distribution of the risks from multiple pollutants within the region was developed in this paper. An integrated quantitative risk assessment was conducted by utilizing a large amount of information available to explore spatial distribution of risk by single and multiple pollutants, and the magnitude of the overall risk from multiple pollutants based on the current concentrations of pollutants and toxicity data. Two target criteria levels – level I (NOEC/LOEC based) and level II (LC/EC/IC₅₀ based) – were employed, and thus, the regional and sub-regional risks were evaluated according to these two levels. The risk of multiple toxic metals (As, Cd, Cr, Hg and Pb) to a terrestrial ecosystem for the region around the Bohai Sea and the Yellow Sea were evaluated as a case. The total overall ecological risks from heavy metals in the region for level I and level II were 21.73% and 12.53%, respectively. The risks were ranked in the order of Cr > As > Pb > Cd > Hg with Cr posing the greatest ecological risk, which was 61.12% for level I. The top three cities according to the level II ecological risk were Cangzhou > Lianyungang > Panjin, while the top three cities of level I ecological risk were Cangzhou > Panjin > Lianyungang. This method provides a quantitative risk assessment with multiple and clear protection levels for risk management.
Show more [+] Less [-]Microplastics in freshwater river sediments in Shanghai, China: A case study of risk assessment in mega-cities
2018
Peng, Guyu | Xu, Pei | Zhu, Bangshang | Bai, Mengyu | Li, Daoji
Microplastics, which are plastic debris with a particle diameter of less than 5 mm, have attracted growing attention in recent years. Its widespread distributions in a variety of habitats have urged scientists to understand deeper regarding their potential impact on the marine living resources. Most studies on microplastics hitherto are focused on the marine environment, and research on risk assessment methodology is still limited. To understand the distribution of microplastics in urban rivers, this study investigated river sediments in Shanghai, the largest urban area in China. Seven sites were sampled to ensure maximum coverage of the city's central districts, and a tidal flat was also included to compare with river samples. Density separation, microscopic inspection and μ-FT-IR analysis were conducted to analyze the characteristics of microplastics and the type of polymers. The average abundance of microplastics in six river sediment samples was 802 items per kilogram of dry weight. The abundance in rivers was one to two orders of magnitude higher than in the tidal flat. White microplastic spheres were most commonly distributed in river sediments. Seven types of microplastics were identified, of which polypropylene was the most prevailing polymers presented. The study then conducted risk assessment of microplastics in sediments based on the observed results, and proposed a framework of environmental risk assessment. After reviewing waste disposal related legislation and regulations in China, this study conclude that in situ data and legitimate estimations should be incorporated as part of the practice when developing environmental policies aiming to tackle microplastic pollution.
Show more [+] Less [-]Source tagging modeling study of regional contributions to acid rain in summer over Liaoning Province, Northeastern China
2018
Gbaguidi, Alex E. | Wang, Zifa | Wang, Wei | Yang, Ting | Chen, Huan-Sheng
Strong acid rain was recently observed over Northeastern China, particularly in summer in Liaoning Province where alkaline dust largely neutralized acids in the past. This seems to be related to the regional transboundary pollution and poses new challenges in acid rain control scheme in China. In order to delve into the regional transport impact, and quantify its potential contributions to such an “eruption” of acid rain over Liaoning, this paper employs an online source tagging model in coupling with the Nested Air Quality Prediction Modeling System (NAQPMS). Validation of predictions shows the model capability in reproducing key meteorological and chemical features. Acid concentration over Liaoning is more pronounced in August (average of 0.087 mg/m³) with strong pollutant import from regional sources against significant depletion of basic species. Seasonal mean contributions from regional sources are assessed at both lower and upper boundary layers to elucidate the main pathways of the impact of regional sources on acid concentration over Liaoning. At the upper layer (1.2 km), regional sources contribute to acid concentration over Liaoning by 67%, mainly from Shandong (16%), Hebei (13%), Tianjin (11%) and Korean Peninsula (9%). Identified main city-receptors in Liaoning are Dandong, Dalian, Chaohu, Yingkou, Liaoyang, Jinfu, Shengyang, Panjin, Tieling, Benxi, Anshan and Fushun. At lower layer (120 m) where Liaoning local contribution is dominant (58%), regional sources account for 39% in acid concentration. However, inter-municipal acid exchanges are prominent at this layer and many cities in Liaoning are revealed as important sources of local acid production. Seasonal acid contribution average within 1.2 km-120 m attains 55%, suggesting dominance of vertical pollutant transport from regional sources towards lower boundary layer in Liaoning. As direct environmental implication, this study provides policy makers with a perspective of regulating the regional transboundary environmental impact assessment in China with application to acid rain control.
Show more [+] Less [-]Uptake kinetics of pesticides chlorpyrifos and tebuconazole in the earthworm Eisenia andrei in two different soils
2018
Svobodová, Markéta | Šmídová, Klára | Hvězdová, Martina | Hofman, Jakub
Agriculture is today indispensably connected with enormous use of pesticides. Despite tough regulation, their entrance into soil cannot be excluded and they might enter soil organisms and plants and continue further to terrestrial food chains. This study was conducted to investigate the bioaccumulation of two pesticides currently used in large amounts, the insecticide chlorpyrifos (CLP) and the fungicide tebuconazole (TBZ). Their detailed uptake kinetics in the model earthworm species Eisenia andrei were measured in two arable soils differing in organic carbon content (1.02 and 1.93% respectively). According to our results, a steady state was reached after 3–5 days for both pesticides and soils. The values of bioaccumulation factors calculated at the steady state ranged from 4.5 to 6.3 for CLP and 2.2–13.1 for TBZ. Bioaccumulation factors were also calculated as the ratio of uptake and elimination rate constants with results comparable with steady-state bioaccumulation factors. The results suggested that the degradation and bioaccumulation of tested compounds might be influenced by other factors than only total organic carbon (e.g. clay content). The lower Koc and hydrophobicity of TBZ relative to CLP probably led to higher availability of TBZ through pore water exposure. On the other hand, CLP's higher hydrophobicity probably caused an increase in availability by its additional uptake via ingestion. To enable a proper ecological risk assessment of current pesticides in soils, it is necessary to accurately determine their bioaccumulation in soil invertebrates. We believe that our study not only brings such information for two specific pesticides but also addresses key methodological issues in this area.
Show more [+] Less [-]Ecological risk assessment of a contaminated stream using multi-level integrated biomarker response in Carassius auratus
2018
Samanta, Palas | Im, Hyungjoon | Na, Joorim | Jung, Jinho
The goal of this study was to evaluate the adverse effects of wastewater effluents on freshwater crucian carp, Carassius auratus, inhabiting Sincheon stream using the integrated biomarker response (IBR) at the genotoxic (micronucleus [MN] test), oxidative stress (activity of catalase [CAT] and glutathione S-transferase [GST], and level of lipid peroxidation [LPO]), histopathological (degree of tissue changes [DTC]), and physiological (condition factor [CF] and liver somatic index [LSI]) levels. The CF and LSI were significantly (p < 0.05) enhanced in fish from downstream sites (DS1 and DS2) as compared to that of upstream (US) fish samples. Moreover, a significant increase in morphometric indices (DTC) was observed in C. auratus collected from downstream sites (p < 0.05) and histopathological responses showed the degree of pathogenicity in the order of liver > kidney > gills. The activities of CAT, GST, and LPO in fish from the DS1 and DS2 sites were notably increased in gills, liver, and kidney compared to that of fish from the US site. Additionally, the MN test level in C. auratus from the DS1 and DS2 were significantly increased (p < 0.05) when compared with that of the US site. Considering the higher bioaccumulation of Cd, Co, Cr, Mn, Ni, and Pb in gills, liver, and kidney of C. auratus collected from downstream sites compared to that of the upstream site (p < 0.05), the observed toxicity was likely attributable to metal accumulation. The multi-level IBR index was higher at the DS1 site (15.08) than at the DS2 (1.02) and the reference US (0.00) sites. Therefore, these findings demonstrated that wastewater effluent discharge induces significant DNA damage, oxidative stress, and tissue injuries in C. auratus and suggested that the multi-level IBR approach should be used to quantify these effects on fish in streams and rivers.
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