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Physiological responses of wheat planted in fluvo-aquic soils to di (2-ethylhexyl) and di-n-butyl phthalates
2019
Gao, Minling | Liu, Yu | Dong, Youming | Song, Zhengguo
Di (2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) are important pollutants that contaminate agricultural soils. We determined the effects of di (2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) on the production of reactive oxygen species, photosynthesis, and activity of antioxidant enzymes in wheat planted in fluvo-aquic soils. DBP- and DEHP-induced oxidative stress decreased the values of the photosynthetic/fluorescence parameters (except for intercellular carbon dioxide concentration) and chlorophyll content at the seedling, jointing, and booting stages. Moreover, the non-stomatal factor responsible for the net decrease in photosynthetic efficiency was identified as the decrease in fluorescence resulting from the decreased amount of chlorophyll a returning from the excited to the ground energy state. The content of superoxide anions and hydrogen peroxide in wheat leaves and roots increased with increasing DBP and DEHP supplementation, compared to the control. Antioxidant enzyme activities in the leaves and roots at the seedling stage increased at DBP and DEHP levels of 10 and 20 mg kg⁻¹, respectively, and the enzyme activities at the jointing and booting stages increased with increasing concentrations of the chemicals, compared to the control. These results demonstrated that increased levels of antioxidant enzymes play a significant role in protecting plant growth under DBP and DEHP stress.
Show more [+] Less [-]Long-term N and S addition and changed litter chemistry do not affect trembling aspen leaf litter decomposition, elemental composition and enzyme activity in a boreal forest
2019
Wang, Qi | Kwak, Jin-Hyeob | Choi, Woo-Jung | Chang, Scott X.
The effect of long-term nitrogen (N) and sulfur (S) deposition on litter mass loss and changes in carbon (C), N, and S composition and enzyme activities during litter decomposition was investigated in a boreal forest. This study included four N × S treatments: control (CK), N application (30 kg N ha−1 yr−1), S application (30 kg S ha−1 yr−1), and N plus S application (both at 30 kg ha−1 yr−1). Two experiments were conducted for 22 months: 1) a common litter decomposition experiment with litter bags containing a common litter (same litter chemistry) and 2) an in-situ litter decomposition experiment with litter from each treatment plot (and thus having different litter chemistry). Litterbags were placed onto the four treatment plots to investigate the direct effect of N and S addition and the combined effect of N and/or S addition and litter chemistry on litter decomposition, respectively. Regardless of the source of litter, N and/or S addition affected C, N and S composition at a certain period of the experiment but did not affect litter mass loss and enzyme activity throughout the experiment, indicating that the N and S addition rates were below the critical level required to affect C and N cycling in the studied ecosystem. However, the greater change in N composition per unit of litter mass loss in the N addition treatment than in the other treatments in the common litter but not in the in-situ litter experiment, suggests that the effect of N addition on N loss and retention depends on the initial litter chemistry. We conclude that the studied N and S addition rates did not affect litter decomposition and elemental cycling in the studied forest ecosystem even though the N and S addition rates were much greater than their ambient deposition rates.
Show more [+] Less [-]Integrated thyroid endocrine disrupting effect on zebrafish (Danio rario) larvae via simultaneously repressing type II iodothyronine deiodinase and activating thyroid receptor-mediated signaling following waterborne exposure to trace azocyclotin
2019
Jiao, Fang | Qiao, Kun | Jiang, Yao | Li, Shuying | Zhao, Jinghao | Gui, Wenjun
As a widely used organotin acaricide nowadays, azocyclotin (ACT) could induce thyroidal endocrine disruption in fishes and amphibians, but its dominant disrupting mode remains unknown. In this study, zebrafish were firstly exposed to ACT (0.18–0.36 ng/mL) from 2 hpf (hours post fertilization) to 30 dpf (days post fertilization), and a series of developmental toxicological endpoints and thyroid hormones were measured. Result showed that no developmental toxicity to zebrafish was found in 0.18 and 0.24 ng/mL groups except decreased body weight (30 dpf, 0.24 ng/mL). However, exposed to 0.36 ng/mL ACT led to reductions in heartbeat (48 hpf), hatching rate (72 hpf) and bodyweight (30 dpf). General tendencies of decreases in free T3 but increases in free T4 and reductions in ratio of free T3/T4 were also found, inferring that type II deiodinase (Dio2) was repressed. This inference was confirmed by Western analysis that Dio2 expression reduced by 42.7% after 0.36 ng/mL ACT treatment. Moreover, RNA-Seq analysis implied that exposed to 0.36 ng/mL ACT altered the genome-wide gene expression profiles of zebrafish. Totally 5660 genes (involving 3154 down-regulated and 2596 up-regulated genes) were differentially expressed, and 13 deferentially expressed genes including down-regulated dio2 were significantly enriched in thyroid hormone signaling pathway. Subsequently, an in vitro thyroid receptor-reporter gene assay using GH3 cells was performed to further explore the potential disrupting mechanism. Result showed that luciferase activity slightly increased after exposure to ACT alone or ACT combined with low level T3, but was suppressed when combined with high level T3. It indicted there probably existed a competitive relationship in some extent between ACT and T3 in vivo. Overall, the present study provided preliminary evidences that long-term exposure to trace ACT repressed Dio2 expression, declined T3 and then activated thyroid receptor-mediated signaling, thereby leading to integrated thyroid endocrine disruption in zebrafish larvae.
Show more [+] Less [-]Responses of soil microbial communities and their network interactions to saline-alkaline stress in Cd-contaminated soils
2019
Wang, Meng | Chen, Shibao | Chen, Li | Wang, Duo
Land degradation by salinization and sodification changes soil function, destroys soil health, and promotes bioaccumulation of heavy metals in plants, but little is known about their fundamental mechanisms in shaping microbial communities and regulating microbial interactions. In this study, we explored the impact of saline-alkaline (SA) stress on soil bacterial and fungal community structures in different Cd-contaminated soils of Dezhou, Baoding, Xinxiang, Beijing and Shenyang cities from the North China Plain, China. Increased soil salinity and alkalinity enhanced Cd availability, indicated by significant increases in available Cd2+ in soil solution of 34.1%–49.7%, soil extractable Cd of 32.0–51.6% and wheat root Cd concentration of 24.5%–40.2%, as well as decreased activities of antioxidative enzymes of wheat root when compared with CK (no extra neutral or alkaline salts added). Soil bacteria were more active in response to the SA stress than fungi, as the significant structural reorganization of soil bacterial microbiota rather than fungal microbiota between SA and CK treatments was illustrated by principal component analysis. Adding neutral and alkaline salts enriched oligotrophic and haloalkaliphilic taxa in the Sphingobacteriaceae, Cellvibrionaceae, and Caulobacteraceae bacterial families, but decreased some Acidobacteria such as subgroup 6_norank, which was a sensitive biomarker that responded only to Cd contamination in CK-treated soils. Conversely, fungi were more sensitive to soil differences than bacteria: the composition of the fungal community was significantly different among different soil types. Phylogenetic molecular ecological network (pMEN) analysis further indicated that the microbial community structure and network interactions were altered to strengthen the adaptability of microorganisms to SA stress; the changes in structure and network interactions were proposed to contribute to competitive interactions. Most of the keystone genera identified in SA-treated soils, such as Blastococcus, Gemmatimonas, RB41, or Candida, had relatively low abundances (<1%), indicating their disproportionate ecological roles in triggering resistance or tolerance to SA stress and Cd toxicity.
Show more [+] Less [-]Species-specific debromination of polybromodiphenyl ethers determined by deiodinase activity in fish
2019
Luo, Yuan-Lai | Luo, Xiao-Jun | Ye, Mei-Xia | Lin, Lan | Zeng, Yan-Hong | Mai, Bi-Xian
A combination of previous studies and the present study indicated species-specific debromination of polybromodiphenyl ethers (PBDEs) in teleost fish. Three situations of debromination were found, namely rapid debromination represented by debromination of BDE 99 to BDE 47 observed in common carp, tilapia, crucian carp, and oscar fish; slow debromination represented by debromination of BDE 99 to BDE 49 observed in the abovementioned fish and rainbow trout, salmon, and snakehead; and no or minor debromination observed in catfish. The results of experiments on cofactors, inhibitors, and substrate competitors indicated that activities of outer ring deiodinase of 3, 3′, 5′-triiodothyronine (type I deiodinase), which cannot be inhibited by 6-propyl-2-thiouracil, were responsible for the rapid debromination, and the outer ring deiodinase of thyroxine (type II deiodinase) regulated the slow debromination. The debromination of BDE 99 to BDE 49 was more common, but occurred at a much lower rate (approximately 100 times lower) than the debromination of BDE 99 to BDE 47. This was because the activity of type II deiodinase was nearly two orders of magnitude lower than that of type I deiodinase in the fish species studied. Further studies on debromination of PBDEs and properties of deiodinase in more species are needed to confirm the hypothesis.
Show more [+] Less [-]The lipid metabolism alteration of three spirocyclic tetramic acids on zebrafish (Danio rerio) embryos
2019
Zhang, Jie | Qian, Le | Teng, Miaomiao | Mu, Xiyan | Qi, Suzhen | Chen, Xiangguang | Zhou, Yimeng | Cheng, Yi | Pang, Sen | Li, Xuefeng | Wang, Chengju
Spirocyclic tetramic acids are widely used in controlling phytophagous mite species throughout the world. the data set is incomplete and provides insufficient evidence for drawing the same conclusion for fish. To fill the gap whether these acaricides alter lipid metabolism on vertebrates, zebrafish embryos exposed to a series concentration of pesticides, the developmental effects, enzyme activities and levels of gene expression were assessed, battery of biomarker utilized by the integrated biomarker response (IBRv2) model. The 96 h-LC₅₀ of spirodiclofen, spiromesifen and spirotetramat were 0.14, 0.12 and 5.94 mg/L, respectively. Yolk sac deformity, pericardial edema, spinal curvature and tail malformation were observed. Three spirocyclic acids were unfavouring the lipid accumulation of by inhibited the acetyl-CoA carboxylase (ACC), fatty acid synthesis (FAS), fatty acid binding proteins (FABP2) and lipoprotein lipase (LPL) activity. The total cholesterol (TCHO) level significantly decreased in the 0.072 mg/L spirodiclofen group and 0.015 and 0.030 mg/L in the spiromesifen groups. No expected change in spirotetramat group on the TCHO and triglycerides (TGs) levels for any of the treatments. The mRNA levels of the genes related to lipid metabolism also significantly altered. In both spirodiclofen and spiromesifen, ACC achieved the highest scores among a battery of biomarkers using integrated biomarker response (IBRv2). The results suggest that spiromesifen was the most toxic for embryos development and spirodiclofen was the most toxic for lipid metabolism in embryos. The 0.07 mg/L of spirodiclofen, 0.05 mg/L of spiromesifen and 2.00 mg/L would cause malformation on zebrafish embryos. This study will provide new insight that fatty acid metabolism may be a suitable biomarker for the spirocyclic tetramic acids in fish species.
Show more [+] Less [-]Accumulation of pollutants in nestlings of an endangered avian scavenger related to territory urbanization and physiological biomarkers
2019
Ortiz-Santaliestra, Manuel E. | Tauler-Ametller, Helena | Lacorte, Silvia | Hernández-Matías, Antonio | Real, Joan | Mateo, Rafael
We monitor pollutant accumulation and investigate associated changes at the physiological level within the population of an obligate avian scavenger, the Egyptian Vulture (Neophron percnopterus), from Catalonia (NE Spain). This population is expanding its range, presumably because of the use of human waste disposal sites as food resource. We hypothesized that habitat urbanization, presumably associated with feeding from human wastes, could influence the accumulation of persistent organic pollutants and metals. The aim of this study was to explore the relationship between accumulated pollutants and biochemical parameters in nestling blood. We used the proportion of urban surface within an 8 km radius of each nest as a proxy to study the relationship between anthropic influence and pollutant accumulation. Observed blood levels of metals, organochlorine pesticides, polychlorinated biphenyls (PCBs), per- and polyfluoroalkylated substances (PFAS) and polybrominated diphenyl ethers (PBDEs) were relatively low, as expected for nestling individuals due to short-term exposures. CB-180 and PBDEs were associated with variations in blood biochemistry parameters; hexa-BDEs appeared positively associated with activities of the enzymes aspartate aminotransferase and lactate dehydrogenase, whereas CB-180 accumulation was associated with an increased activity of creatine phosphokinase and elevated glutathione levels. Increased CB-180 levels were also related to decreased blood concentrations of calcium, cholesterol, α-tocopherol and lutein. A proportion of urban surfaces as low as 6.56% within a radius of 8 km around the nest appears related to the accumulation of CB-180, the majority of analysed PFAS and of PBDE congeners 99 and 209, and increased urbanization was also associated with decreased plasma levels of α-tocopherol and carotenoids. These associations suggest that changes in blood profiles of vitamins, carotenoids or other analytes, despite related to increased plasma levels of CB-180, would be consequence of exploitation of artificial food sources rather than of a direct effect of the pollutants.
Show more [+] Less [-]Exposure to nickel oxide nanoparticles insinuates physiological, ultrastructural and oxidative damage: A life cycle study on Eisenia fetida
2019
Adeel, Muhammad | Ma, Chuanxin | Ullah, Sana | Rizwan, Muhammad | Hao, Yi | Chen, Chunying | Jilani, Ghulam | Shakoor, Noman | Li, Mingshu | Wang, Lihong | Tsang, Daniel C.W. | Rinklebe, Jörg | Rui, Yukui | Xing, Baoshan
Although, health and environmental hazards of Ni are ironclad; however, that of Nickle oxide nanoparticles (NiO-NPs) are still obscure. Therefore, impact of NiO-NPs exposure (0, 5, 50, 200, 500 and 1000 mg kg⁻¹ soil) on the earthworm (Eisenia fetida) survival (at 28th day), reproduction (at 56th day), histopathology, ultrastructures, antioxidant enzymes and oxidative DNA damage was appraised in full life cycle study. Lower concentrations of NiO-NPs (5, 50 and 200) did not influence the survival, reproduction and growth rate of adult worms significantly. However, reproduction reduced by 40–50% with 500 and 1000 mg kg⁻¹ exposure, which also induced oxidative stress leading to DNA damage in earthworms. Ultrastructural observation and histology of earthworms exposed to higher NiO-NPs concentrations revealed abnormalities in epithelium layer, microvilli and mitochondria with underlying pathologies of epidermis and muscles, as well as adverse effects on the gut barrier. To the best of our knowledge, this is the first study unveiling the adverse effects of NiO-NPs on a soil invertebrate (Eisenia fetida). Our findings clue towards looking extensively into the risks of NiO-NPs on soil organisms bearing agricultural and environmental significance.
Show more [+] Less [-]Alleviation of the effect of salinity on growth and yield of strawberry by foliar spray of selenium-nanoparticles
2019
Zahedi, Seyed Morteza | Abdelrahman, Mostafa | Hosseini, Marjan Sadat | Hoveizeh, Narjes Fahadi | Tran, Lam-son Phan
The present study investigated the beneficial role of selenium-nanoparticles (Se-NPs) in mitigating the adverse effects of soil-salinity on growth and yield of strawberry (Fragaria × ananassa Duch.) plants by maneuvering physiological and biochemical mechanisms. The foliar spray of Se-NPs (10 and 20 mg L⁻¹) improved the growth and yield parameters of strawberry plants grown on non-saline and different saline soils (0, 25, 50 and 75 mM NaCl), which was attributed to their ability to protect photosynthetic pigments. Se-NPs-treated strawberry plants exhibited higher levels of key osmolytes, including total soluble carbohydrates and free proline, compared with untreated plants under saline conditions. Foliar application of Se-NPs improved salinity tolerance in strawberry by reducing stress-induced lipid peroxidation and H₂O₂ content through enhancing activities of antioxidant enzymes like superoxide dismutase and peroxidase. Additionally, Se-NPs-treated strawberry plants showed accumulation of indole-3-acetic acid and abscisic acid, the vital stress signaling molecules, which are involved in regulating different morphological, physiological and molecular responses of plants to salinity. Moreover, the enhanced levels of organic acids (e.g., malic, citric and succinic acids) and sugars (e.g., glucose, fructose and sucrose) in the fruits of Se-NPs-treated strawberry plants under saline conditions indicated the positive impacts of Se-NPs on the improvement of fruit quality and nutritional values. Our results collectively demonstrate the definite roles of Se-NPs in management of soil salinity-induced adverse effects on not only strawberry plants but also other crops.
Show more [+] Less [-]Remediation performance and mechanism of hexavalent chromium in alkaline soil using multi-layer loaded nano-zero-valent iron
2019
Hou, Siyu | Wu, Bin | Peng, Dinghua | Wang, Ziru | Wang, Yiyang | Xu, Heng
Remediation of soil chromium (Cr) pollution is becoming more and more urgent. In this study, a multi-loaded nano-zero-valent iron (nZVI) material (CNH) was prepared by carboxymethyl cellulose (CMC) and humic acid (HA) as dispersant and support agent, respectively, and the remediation effect of CNH, HA and CN (CNH without HA) for Cr contaminated soil was investigated within 90 d cycle. After 7 d treatment of CNH, the HOAc-extractable Cr decreased significantly. After the 90 d remediation, the HOAc-extractable Cr decreased most in the treatment of 3% CNH, about 74.48% lower than control. All treatments eventually caused different decline of soil pH, with a range of 0.12–0.54, in which the CNH treatment group had the least depression. HA loading significantly weakened the toxicity of nZVI, resulting in the higher soil microbial quantity and enzyme activities compared with CN. Additionally, the improvement of soil microecology by CNH and HA was positively correlated with the ratio of application, while CN was negatively correlated (except FDA enzyme activity) with these indexes. These results emphasized the potential of the synthesized CNH as a promising material to remediate Cr contaminated soil. Furthermore, details of possible mechanistic insight into the Cr remediation were carefully discussed.
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