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Ammonium and nitrate tolerance in lichens
2010
Since lichens lack roots and take up water, solutes and gases over the entire thallus surface, these organisms respond more sensitively to changes in atmospheric purity than vascular plants. After centuries where effects of sulphur dioxide and acidity were in the focus of research on atmospheric chemistry and lichens, recently the globally increased levels of ammonia and nitrate increasingly affect lichen vegetation and gave rise to intense research on the tolerance of lichens to nitrogen pollution. The present paper discusses the main findings on the uptake of ammonia and nitrate in the lichen symbiosis and to the tolerance of lichens to eutrophication. Ammonia and nitrate are both efficiently taken up under ambient conditions. The tolerance to high nitrogen levels depends, among others, on the capability of the photobiont to provide sufficient amounts of carbon skeletons for ammonia assimilation. Lowly productive lichens are apparently predisposed to be sensitive to excess nitrogen.
Show more [+] Less [-]Eco-restoration: Simultaneous nutrient removal from soil and water in a complex residential–cropland area
2010
Wu, Yonghong | Kerr, Philip G. | Hu, Zhengyi | Yang, Linzhang
An eco-restoration system to remove excess nutrients and restore the agricultural ecosystem balance was proposed and applied from August 2006 to August 2008 in a residential-cropland complex area (1.4 × 105 m2) in Kunming, western China, where the self-purifying capacity of the agricultural ecosystem had been lost. The proposed eco-restoration system examined includes three main foci: farming management, bioremediation, and wastewater treatment. The results showed that the removal efficiencies of total phosphorus (TP) and total nitrogen (TN) from the complex wastewater were 83% and 88%, respectively. The Simpson’s diversity indices of macrophytes and zoobenthos indicated that the system had increased macrophyte and zoobenthic diversity as well as improved growth conditions of the plankton habitats. The results demonstrated that the proposed eco-restoration system is a promising approach for decreasing the output of nutrients from soil, improving agricultural ecosystem health, and minimizing the downstream eutrophication risk for surface waters.
Show more [+] Less [-]Nutrient and microbial dynamics in eutrophying shrimp ponds affected or unaffected by vibriosis
2010
Lemonnier, Hugues | Courties, Claude | Mugnier, Chantal | Torreton, Jean-pascal | Herbland, Alain
A field survey was conducted on two intensive shrimp farms using similar technical practices: one (DF) historically affected by a vibriosis, the other (HC) in which the pathogen has been observed although no mortality event has occurred. Because historical data suggest that eutrophication process may directly or indirectly play a role in the disease outbreak, we focussed our research on its dynamics. A higher variability of the phytoplanktonic compartment linked to an imbalance in the molar N:P ratio was observed in farm DF compared to farm HC, implying a modification on the linkage between the bacteria and phytoplankton compartments at DF. The beginning of the mortality outbreak at DF followed a shift from picoto nanophytoplankton. The organic matter mineralization process at the water-sediment interface may explain the disturbance observed in the water column during eutrophication. The consequences of this disturbance on shrimps' health status and pathogen ecology are discussed. (C) 2009 Elsevier Ltd. All rights reserved.
Show more [+] Less [-]Spatial and temporal extension of eutrophication associated with shrimp farm wastewater discharges in the New Caledonia lagoon
2010
Thomas, Yoann | Courties, Claude | El Helwe, Yasmin | Herbland, Alain | Lemonnier, Hugues
Shrimp farming in New Caledonia typically uses a flow-through system with water exchange rates as a tool to maintain optimum hydrological and biological parameters for the crop. Moreover, the effluent shows hydrobiological characteristics (minerals, phytoplankton biomass and organic matter) significantly higher than that of the receiving environment. Separate surveys were carried out in a bay (CH Bay) with a medium-size intensive farm (30 ha) (PO) and in a mangrove-lined creek (TE Creek) near a larger semi-intensive farm (133 ha) (SO). Net loads of nitrogen exported from the semi-intensive farm and the intensive farm amounted to 0.68 and 1.36 kg ha(-1) day(-1), respectively. At CH Bay, discharge effects were spatially limited and clearly restricted to periods of effluent release. The high residence time at site TE favoured the installation of a feedback system in which organic matter was not exported. Mineralization of organic matter led to the release of nutrients, which in turn, caused in an increased eutrophication of this ecosystem. The study of the pico- and nanophytoplankton assemblages showed (i) a shift in composition from picophytoplankton to nanophytoplankton from offshore towards the coast and (ii) a shift within the picophytoplankton with the disappearance of Prochlorococcus and the increase of picoeucaryotes towards the shoreline. These community changes may partially be related to a nitrogen enrichment of the environment by shrimp farm discharges. Thus, in view of the recent addition of the New Caledonian lagoon to the UNESCO World Heritage list, the data presented here could be a first approach to quantify farm discharges and evaluate their impact on the lagoon. (C) 2010 Elsevier Ltd. All rights reserved.
Show more [+] Less [-]Phosphorus Fractions Transformation in Sediments Before and After Cyanobacterial Bloom: Implications for Reduction of Eutrophication Symptoms in Dam Reservoir
2010
Trojanowska, Adriana Anna | Izydorczyk, Katarzyna
In order to observe the spatial phosphorus (P) fractions transformations in sediments in relation to bacterial abundance and enzyme hydrolysing organic P-alkaline phosphatase (APA), samples from 35 stations from eutrophic Sulejow Reservoir were taken in spring after flood and in summer after cyanobacterial bloom breakdown. The results show pronounced fluctuations: decrease of average total P in sediments, despite organic matter delivery after cyanobacterial bloom, in parallel with increase of labile P (8.3%) and Ca-bounded P (16.6%) fractions and decline of organic P fraction (28.5%). Higher alkaline activity in sediments in the spring delivered nutrients to water column and supported cyanobacterial bloom development during the summer. Positive correlation between APA and organic P (r = 0.37, p < 0.01, n = 70) and negative with labile inorganic P (r = −0.44, p < 0.01, n = 70) in sediments proved significant role of the APA in phosphorus transformation in sediments and internal loading in the reservoir. During summer, APA was significantly related to bacterial number (r = 0.36, p < 0.01, n = 35) and bacterial abundance was correlated to organic matter content (r = 0.36, p < 0.01, n = 35). Such pattern of temporal variations of P transformation in sediments indicates order of solutions for enhancement of recultivation effects of eutrophic dam reservoirs: (1) reduction of organic matter supply in spring and (2) sediment inactivation during summer.
Show more [+] Less [-]Mercury Flux to Sediments of Lake Tahoe, California-Nevada
2010
Drevnick, Paul E. | Shinneman, Avery L. C. | Lamborg, Carl H. | Engstrom, Daniel R. | Bothner, Michael H. | Oris, James T.
We report estimates of mercury (Hg) flux to the sediments of Lake Tahoe, California-Nevada: 2 and 15-20 µg/m²/year in preindustrial and modern sediments, respectively. These values result in a modern to preindustrial flux ratio of 7.5-10, which is similar to flux ratios recently reported for other alpine lakes in California, and greater than the value of 3 typically seen worldwide. We offer plausible hypotheses to explain the high flux ratios, including (1) proportionally less photoreduction and evasion of Hg with the onset of cultural eutrophication and (2) a combination of enhanced regional oxidation of gaseous elemental Hg and transport of the resulting reactive gaseous Hg to the surface with nightly downslope flows of air. If either of these mechanisms is correct, it could lead to local/regional solutions to lessen the impact of globally increasing anthropogenic emissions of Hg on Lake Tahoe and other alpine ecosystems.
Show more [+] Less [-]Adverse Effects of Ammonia on Nitrification Process: the Case of Chinese Shallow Freshwater Lakes
2010
Chen, Guoyuan | Cao, Xiuyun | Song, Chunlei | Zhou, Yiyong
Nitrification is a process in which ammonia is oxidized to nitrite (NO ₂ ⁻ ) that is further oxidized to nitrate (NO ₃ ⁻ ). The relations between these two steps and ambient ammonia concentrations were studied in surface water of Chinese shallow lakes with different trophic status. For the oxidations of both ammonia and NO ₂ ⁻ , more eutrophic lakes generally showed significantly higher potential and actual rates, which was linked with excessive ammonia concentrations. Additionally, both potential and actual rates for ammonia oxidation were higher than those for NO ₂ ⁻ oxidation in the more eutrophic lakes, while in the lakes with lower trophic status, both potential and actual rates for ammonia oxidation were almost equivalent to those for NO ₂ ⁻ oxidation. This can be explained by the excessive unionized ammonia (NH₃) concentration that inhibits nitrite-oxidizing bacteria in the more eutrophic lakes. The laboratory experiment with different ammonia concentrations, using the surface water in a eutrophic lake, showed that ammonia oxidation rates were proportional to the ammonia concentrations, but NO ₂ ⁻ oxidation rates did not increase in parallel. Furthermore, NO ₂ ⁻ oxidation was less associated with particles in natural water of the studied lakes. Without effective protection, it would be selectively inhibited by the excessive ammonia in hypereutrophic lakes, resulting in NO ₂ ⁻ accumulation. Shortly, the increased concentrations of ammonia cause a misbalance between the NO ₂ ⁻ -producing and the NO ₂ ⁻ -consuming processes, thereby exacerbating the lake eutrophication.
Show more [+] Less [-]Control of Algal Scum Using Top-Down Biomanipulation Approaches and Ecosystem Health Assessments for Efficient Reservoir Management
2010
An, Kwang-Guk | Lee, Jae-Yon | Kumar, Hema K. | Lee, Sang-Jae | Hwang, Soon-Jin | Kim, Baik-Ho | Park, Young-Seuk | Shin, Kyung-Hoon | Park, Sangkyu | Um, Han-Yong
The objectives of this study were algal control and health assessments in a temperate eutrophic reservoir. Laboratory and mesocosm-scale in situ top-down biomanipulation experiments using planktivorous fishes and filter-feeding macroinvertebrates were conducted along with identification of the limiting nutrient using nutrient enrichment bioassays (NEBs), and ecosystem health evaluation based on the modified index of biological integrity model (Reservoir Ecosystem Health Assessment; REHA). Nutrients and N/P ratio analyses during 5 years revealed that the reservoir was in a eutrophic-hypertrophic state and that the key limiting nutrients, based on the NEBs, varied among seasons. Reservoir trophic guilds indicated declines in sensitive and endemic fish species and dominance of tolerant omnivores. Model values from multimetric REHAs averaged 25.8, indicating that the ecological health was in “fair to poor” condition. Overall microcosm biomanipulation tests suggested that macroinvertebrates, specifically Palaemon paucidens and Caridina denticulata, were effective candidates for phytoplankton control, compared to fishes. In situ mesocosm experiments revealed the highest removal rates with bluegreen algae and a phytoplankton size fraction of 2-19 μm (R e > 90%, Mann-Whitney U = 64.5-74.0, p < 0.01), the dominant fractions in the reservoir. Our biomanipulation technique may provide a key tool for efficient management and restoration of eutrophied reservoirs.
Show more [+] Less [-]Effects of Elevated CO₂ on Growth and Nutrient Uptake of Eichhornia crassipe Under Four Different Nutrient Levels
2010
Liu, Jun-Zhi | Ge, Ya-Ming | Zhou, Yu-Fei | Tian, Guang-Ming
Although the climate change effects on plants have been a focus for more than two decades, such effects on aquatic species remain largely unknown. To evaluate the potential effects of elevated CO₂ on growth and nutrient uptake of Eichhornia crassipe Solms (commonly known as water hyacinth, the world's most significant invasive aquatic weed), plants were grown at two CO₂ concentrations (380 and 800 ppm) combined with four nutrient levels (oligo-, meso-, eu-, and hypertrophic) for 2 months. Overall, elevated CO₂ consistently enhanced plant growth at all nutrient levels, indicating more infestations of water hyacinth in future natural eutrophic waters. Moreover, the enhancement extent varied among nutrient availabilities, being more in eu- and hypertrophic levels and less in meso- and oligotrophic levels. Furthermore, the CO₂ enrichment-deduced assimilation was allocated more to plant roots than shoots which would improve the nutrient absorption capacity and mostly transferred to offspring ramets rather than maintained at the mother ramet which would benefit the vegetative reproduction. Finally, under elevated CO₂, although the nitrogen (N) and phosphorus (P) contents of E. crassipe slightly decreased which might mean increased difficulties in preventing its infestation by reducing N and/or P in eutrophic waters, the total N and P accumulation increased suggesting higher bioremediation efficiency of using water hyacinth for water eutrophication.
Show more [+] Less [-]Detection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS
2010
Wahidulla, Solimabi | Rajamanickam, Yeziel Rani
Background, aim, and scope Mutagenic nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) have been known to arise in the environment through direct emissions from combustion sources and nitration of PAHs, primarily in the atmosphere. In the marine environment, PAHs are one of the classic anthropogenic organic pollutants, while nitrite (NO ₂ ⁻ ) is produced naturally via various biological processes like imbalance in nitrification/denitrification or eutrophication and subsequent oxygen depletion from an oversupply of nutrients. In this paper, we report the formation of PAH-DNA adducts in fish contaminated with PAHs and exposed to NO ₂ ⁻ in the ambient water. Electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis of the bile of the euryhaline fish Oreochromis mossambicus exposed simultaneously to field relevant sublethal concentrations of phenanthrene and NO ₂ ⁻ and collision-induced dissociation of selected ions revealed the presence of DNA-PAH adducts. The present study indicates that, although several high sensitivity techniques have been developed for the analysis of PAH derived DNA adducts, MS/MS has emerged as a powerful tool in the detection and structure elucidation of DNA adducts. Materials and methods Juvenile O. mossambicus from a local estuarine fish farm were used with increasing frequency for carcinogenicity testing and comparative cancer research. The fish were exposed to the alkylating agent phenanthrene in the presence of NO ₂ ⁻ . Composite untreated bile samples after dilution with methanol: water (1:1; v/v) were analyzed by ESI-MS. Results Several adducts could be evidenced in the bile by MS/MS. Deoxyadenosine/deoxyguanosine having a mass in the range of 450-650 amu is detected. In addition, a segment of modified dinucleotide with a mass that corresponds to a dimer consisting of a modified guanosine and a normal guanosine has also been identified in the bile. Discussion The formation of certain types of DNA adducts is a crucial step in the induction of cancer and a primary stage in mutagenesis. Phenanthrene injected by i.p. route led to the transformation of phenanthrene to N-formyl amino phenanthrene-N ⁶-deoxyadenosine adduct, whereas the fish co-exposed to phenanthrene and ambient nitrite metabolizes PAH to mono-, di- as well as trinitro derivatives, which then react with DNA leading to the formation of mainly modified guanosine and adenosine adducts. In the present investigation, dinitrophenanthrene diol epoxide (DNPDE) adduct with guanosine (m/z 587) seems to be the dominant adduct in the mixture, and its presence is shown first as a comparatively less stable adduct, which decomposes to give a more stable N² adduct (m/z 567). Conclusions MS/MS has proved to be useful in the rapid determination and discrimination of structurally different phenanthrene/derivatives DNA adducts in a complex mixture of fish bile co-exposed to phenanthrene and nitrite. However, the nature of metabolites formed is likely determined by the route of PAH administration, and there is a need to further define the early biochemical events of carcinogenesis in these species. Recommendations and perspectives DNA adduct analysis in fish bile offers a promising approach to study the risk of potentiation of anthropogenic chemicals into genotoxic compounds in the presence of nitrite in the marine environment. We believe this is the first report on the formation of DNA-phenanthrene adducts on co-exposure of the fish to PAH and nitrite.
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