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Hair sampling location in harbor seals (Phoca vitulina) affects selenium and mercury concentrations: Implications for study design of trace element determination in pinnipeds
2012
McHuron, Elizabeth A. | Harvey, James T. | Castellini, J Margaret | O’Hara, Todd M.
Hair is used to determine trace elements exposure and status of pinnipeds because it is an excretory route for many elements and can be collected non-lethally. Despite increased use, there have been few studies on how sampling designs and procedures (e.g., hair type, collection site) affect results. The objective of this study was to determine whether concentrations of an essential (selenium; Se) and non-essential element (mercury; Hg) differed between hair samples collected from two body locations on harbor seals (Phoca vitulina). Concentrations of Se and total Hg (THg) differed between mid-dorsal midline and neck samples, and although the absolute differences were relatively small (Δₐbₛₒₗᵤₜₑ Se=0.69μgg⁻¹, Δₐbₛₒₗᵤₜₑ THg=2.86μgg⁻¹), the relative differences were large (Δᵣₑₗₐₜᵢᵥₑ Se=49%, Δᵣₑₗₐₜᵢᵥₑ THg=17%). These differences highlight the need to standardize the collection site for trace element determination in pinnipeds.
Show more [+] Less [-]Removal of Trimethoprim by a Low-Cost Adsorbent: Influence of Operation Conditions
2012
Dominguez-Vargas, Joaquin R. | Carrillo-Perez, Valentin | Gonzalez-Montero, Teresa | Cuerda-Correa, Eduardo M.
A low-cost carbon black has been used as an adsorbent for the removal of trimethoprim (TMP) from aqueous solution. The kinetic and equilibrium aspects of the adsorption of TMP by this adsorbent were studied. The influence of different operation conditions, namely temperature (20–40 °C), pH (4–8), and ionic strength (0.001–0.1 M) on the removal efficiency of TMP by the adsorbent has been analyzed by applying a statistical design of experiments. Response surface methodology technique was used to optimize TMP removal. Temperature resulted to be the main variable influencing TMP removal, followed by pH. Analysis of variance test reported significance for three of the nine involved variables. An optimum TMP removal was found at pH 9.2, at a temperature of 47 °C and with a value of ionic strength equal to 0.48 M. Under these conditions, a maximum value of removal efficiency equal to 156.2 mg of TMP per gram of adsorbent was attained.
Show more [+] Less [-]Solventless Microextraction Techniques for Determination of Trihalomethanes by Gas Chromatography in Drinking Water
2012
Rosero, Milton Moreano | Aguirre, Mauricio | Pezo, Davinson | Taborda, Gonzalo | Dussán, Carmen | Nerin, Cristina
Three different solventless sample preparation techniques based on microextraction, membrane extraction, and headspace extraction have been developed and optimized for determination of trihalomethanes in drinking water by gas chromatography electron capture detector and mass spectrometry detection. The techniques employed were headspace (HS) solid-phase microextraction, hollow fiber liquid-phase microextraction (HFLPME) and HS extraction. All techniques used were optimized with different experimental designs in order to select the most relevant variables which significantly affect the different processes. The different analytical figures of merit such as limit of detection (LOD), limit of quantification, reproducibility, accuracy, and linear dynamic range were obtained. The new HFLPME method applied used a hollow fiber membrane of polypropylene and the optimized variables were extraction time, extraction temperature, and salting-out effect. The software MODDE 6.0 was used and its design was one central composite on face with a total of 17 runs. The best conditions for the HFLPME were 20 min, 40°C, and 10% NaCl, respectively. The LODs ranged from 0.018 μg·L−1 (for CHClBr2) to 0.049 μg·L−1 (for CHBr3), being this technique the most sensitive one among those studied. Finally, after having optimized the sample preparation techniques and chromatographic conditions, several water samples were taken in two different water treatment plants in Spain (Zaragoza) and Colombia (Viterbo, Caldas). The results obtained are shown and discussed.
Show more [+] Less [-]Electrochemical Degradation of a Real Pharmaceutical Effluent
2012
Domínguez, Joaquín R. | González, Tomás | Palo, P. | Sánchez-Martín, J. | Rodrigo, M. A. | Sáez, C.
In this work, the electrochemical treatment of an effluent from the pharmaceutical industry with boron-doped diamond electrodes was investigated. The electrolyses were carried out in a discontinuous operation mode under galvanostatic conditions, using a bench-scale plant equipped with a single-compartment electrochemical flow cell. The effect of operating conditions, such as current density (from 25.7 to 179.4 mA cm2) and flow rate (from 104.8 to 564.7 cm3 min−1), at residence times between 0 and 570 min, was studied. Design of experiments was used for optimizing the process. The global contribution of operative parameters and evolution of the residence time in TOC removal was studied, and a time of 77 min was obtained in order to evaluate the highest influence of the operative parameters. For this time, ANOVA test reported significance for four of the five involved variables. The current density was found to have a considerable positive effect on TOC removal, whereas the flow rate was found to have a moderate negative effect on target variable.
Show more [+] Less [-]Evaluation of the Influence of Multiple Environmental Factors on the Biodegradation of Dibenzofuran, Phenanthrene, and Pyrene by a Bacterial Consortium Using an Orthogonal Experimental Design
2012
Simarro, Raquel | González, Natalia | Bautista, L Fernando | Molina, M Carmen | Schiavi, Emanuele
For a bioremediation process to be effective, we suggest to perform preliminary studies in laboratory to describe and characterize physicochemical and biological parameters (type and concentration of nutrients, type and number of microorganisms, temperature) of the environment concerned. We consider that these studies should be done by taking into account the simultaneous interaction between different factors. By knowing the response capacity to pollutants, it is possible to select and modify the right treatment conditions to enhance bioremediation.
Show more [+] Less [-]Ozonation of a Carbamazepine Effluent. Designing the Operational Parameters Under Economic Considerations
2012
Palo, Patricia | Domínguez, Joaquín R. | Sánchez-Martín, Jesús
Ozonation of water effluent polluted with carbamazepine an ubiquitous and refractory pharmaceutical contaminant, has been addressed. This paper aims to optimize the remediation process through novel considerations, such as economical aspects of operational costs. To this end, firstly, we have defined an efficiency variable which included not only global efficacy terms (pollutant removal) but also kinetic aspects, which has to do with the ozonation chemical rate. This target variable was involved in a design of experiments that optimized air flow, ozone concentration, and pollutant initial content. An optimum was obtained at 55 L·h ⁻ ¹, 0.4 g·m³, and 18 mg·L ⁻ ¹ respectively.
Show more [+] Less [-]Multiparameter Quantitative Optimization in the Synthesis of a Novel Coagulant Derived from Tannin Extracts for Water Treatment
2012
Beltrán-Heredia, Jesus | Sánchez-Martín, Jesus | Martín-García, Leticia
A novel tannin-based coagulant has been synthesized at lab scale. A multiparameter optimization was performed on the production process, and up to five variables were studied according to the response surface methodology in a face-centered design of experiments which included two temperatures, two pH levels, and the reaction time in the chemical process. The coagulant involved diethanolamine, formaldehyde, and a tannin extract from Acacia mearnsii de Wild. The results revealed an average optimum combination for dye and surfactant removal which was able to remove either Alizarin Violet 3R and sodium dodecylbenzene sulfonate efficiently from water effluents.
Show more [+] Less [-]Avian Urine: Its Potential as a Non-Invasive Biomonitor of Environmental Metal Exposure in Birds
2012
Clapp, J. B. | Bevan, R. M. | Singleton, I.
Current non-invasive biomonitoring techniques to measure heavy metal exposure in free ranging birds using eggs, feathers and guano are problematic because essential metals copper (Cu) and zinc (Zn) deposited in eggs and feathers are under physiological control, feathers accumulate metals from surface contamination and guano may contain faecal metals of mixed bioavailability. This paper reports a new technique of measuring lead (Pb), Cu and Zn in avian urate spheres (AUS), the solid component of avian urine. These metal levels in AUS (theoretically representing the level of metal taken into the bloodstream, i.e. bioavailable to birds) were compared with levels in eggs (yolk and shell), feathers and whole guano from chickens (Gallus gallus domesticus) exposed to a heavy metal-contaminated soil (an allotment soil containing Pb 555 mg kg⁻¹ dry mass (dm), Cu 273 mg kg⁻¹ dm and Zn 827 mg kg⁻¹ dm). The median metal levels (n = 2) in AUS from chickens exposed to this contaminated soil were Pb 208 μg g⁻¹ uric acid, Cu 66 μg g⁻¹ uric acid and Zn: 526 μg g⁻¹ uric acid. Lead concentrations in egg yolk and shell samples (n = 3) were below the limit of detection (<2 mg kg⁻¹), while Cu and Zn were only consistently detected in the yolk, with median values of 3 and 70 mg kg⁻¹ (dm), respectively, restricting the usefulness of eggs as a biomonitor. Feathers (n = 4) had median Pb, Cu and Zn levels respectively of 15, 10 and 140 mg kg⁻¹ (dm), while whole guano samples (n = 6) were 140, 70 and 230 mg kg⁻¹ (dm). Control samples were collected from another chicken flock; however, because they had no access to soil and their diet was significantly higher in Cu and Zn, no meaningful comparison was possible. Six months after site remediation, by top soil replacement, the exposed chickens had median Pb, Cu and Zn levels respectively in whole guano (n = 6) of 30, 20 and 103 mg kg⁻¹ (dm) and in AUS (n = 4) of 147, 16 and 85 μg g⁻¹ uric acid. We suggest the persistent high Pb level in AUS was a consequence of bone mobilised for egg production, releasing chronically sequestered Pb deposits into the bloodstream. In contrast, AUS levels of Cu and Zn (metals under homeostatic control and sparingly stored) had declined, reflecting the lower current exposure. However because pre- and post-remediation samples were measured using different methods carried out at different laboratories, such comparisons should be guarded. The present study showed that metals can be measured in AUS, but no assessment could be made of availability or uptake to the birds because tissue and blood samples were not concomitantly analysed. A major short coming of the study was the inappropriate control group, having no access to uncontaminated soil and being fed a different diet to the exposed birds. Furthermore guano and urine analysis should have been carried out on samples from individual birds, so biological (rather than just technical) variation of metal levels could have been determined. Future studies into using AUS for biomonitoring environmental heavy metals must resolve such experimental design issues.
Show more [+] Less [-]Modeling and optimization of trihalomethanes formation potential of surface water (a drinking water source) using Box–Behnken design
2012
Singh, Kunwar P. | Rai, Premanjali | Pandey, Priyanka | Sinha, Sarita
PURPOSE: The present research aims to investigate the individual and interactive effects of chlorine dose/dissolved organic carbon ratio, pH, temperature, bromide concentration, and reaction time on trihalomethanes (THMs) formation in surface water (a drinking water source) during disinfection by chlorination in a prototype laboratory-scale simulation and to develop a model for the prediction and optimization of THMs levels in chlorinated water for their effective control. METHODS: A five-factor Box–Behnken experimental design combined with response surface and optimization modeling was used for predicting the THMs levels in chlorinated water. The adequacy of the selected model and statistical significance of the regression coefficients, independent variables, and their interactions were tested by the analysis of variance and t test statistics. RESULTS: The THMs levels predicted by the model were very close to the experimental values (R 2 = 0.95). Optimization modeling predicted maximum (192 μg/l) TMHs formation (highest risk) level in water during chlorination was very close to the experimental value (186.8 ± 1.72 μg/l) determined in laboratory experiments. The pH of water followed by reaction time and temperature were the most significant factors that affect the THMs formation during chlorination. CONCLUSION: The developed model can be used to determine the optimum characteristics of raw water and chlorination conditions for maintaining the THMs levels within the safe limit.
Show more [+] Less [-]Combination of beehive matrices analysis and ant biodiversity to study heavy metal pollution impact in a post-mining area (Sardinia, Italy)
2012
Satta, Alberto | Verdinelli, Marcello | Ruiu, Luca | Buffa, Franco | Salis, Severyn | Sassu, Antonio | Floris, Ignazio
Mining activities represent a major source of environment contamination. The aim of this study was to evaluate the use of bees and ants as bioindicators to detect the heavy metal impact in post-mining areas. A biomonitoring programme involving a combination of honeybee hive matrices analysis and ant biodiversity survey was conducted over a 3-year period. The experimental design involved three monitoring stations where repeated sampling activities focused on chemical detection of cadmium (Cd), chrome (Cr) and lead (Pb) from different matrices, both from hosted beehives (foraging bees, honey and pollen) and from the surrounding environment (stream water and soil). At the same time, ant biodiversity (number and abundance of species) was determined through a monitoring programme based on the use of pitfall traps placed in different habitats inside each mining site. The heavy metal content detected in stream water from the control station was always below the analytical limit of quantification. In the case of soil, the content of Cd and Pb from the control was lower than that of mining sites. The mean heavy metal concentrations in beehive matrices from mining sites were mainly higher than the control, and as a result of regression and discriminant analysis, forager bee sampling was an efficient environmental pollution bioindicator. Ant collection and identification highlighted a wide species variety with differences among habitats mostly associated with vegetation features. A lower variability was observed in the polluted landfill characterised by lack of vegetation. Combined biomonitoring with forager bees and ants represents a reliable tool for heavy metal environmental impact studies.
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