The plastic mulch films used in agriculture are considered to be a major source of the plastic residues found in soil. Mulching with low-density polyethylene (LDPE) is widely practiced and the resulting macro- and microscopic plastic residues in agricultural soil have aroused concerns for years. Over the past decades, a variety of biodegradable (Bio) plastics have been developed in the hope of reducing plastic contamination of the terrestrial ecosystem. However, the impact of these Bio plastics in agroecosystems have not been sufficiently studied. Therefore, we investigated the impact of macro (around 5 mm) and micro (<1 mm) sized plastic debris from LDPE and one type of starch-based Bio mulch film on soil physicochemical and hydrological properties. We used environmentally relevant concentrations of plastics, ranging from 0 to 2% (w/w), identified by field studies and literature review. We studied the effects of the plastic residue on a sandy soil for one month in a laboratory experiment. The bulk density, porosity, saturated hydraulic conductivity, field capacity and soil water repellency were altered significantly in the presence of the four kinds of plastic debris, while pH, electrical conductivity and aggregate stability were not substantially affected. Overall, our research provides clear experimental evidence that microplastics affect soil properties. The type, size and content of plastic debris as well as the interactions between these three factors played complex roles in the variations of the measured soil parameters. Living in a plastic era, it is crucial to conduct further interdisciplinary studies in order to have a comprehensive understanding of plastic debris in soil and agroecosystems.

The objective of this work was to test the effect of water limitation on Copaifera langsdorffii Desf. cultivated in mining tailings from the dam rupture in Mariana City, Brazil. Plants were grown in the mining tailing and under two conditions: field capacity (FC) and 50% FC for 60 days. The effects of water restriction on growth, gas exchange, water potential, and leaf anatomy of C. langsdorffii were evaluated. The experimental design was completely randomized with two treatments and 15 replicates, and data was submitted to one-way ANOVA to p < 0.05. Mining tailings showed adequate nutrient levels and the presence of Al, Cd, Pb, and Cr as well as very small particles of 1.19 μm in diameter. The reduction in water availability promoted no changes in the shoot fresh weight, however, increased this parameter for the roots. In addition, water limitation increased plant investment in the root system while reduced biomass allocation to shoots. Lower water levels also increased the root length, number of leaves, and leaf area. However, both water potential and content were not changed by reduced water availability. Lower water levels also increased gas exchange parameters and chlorophyll content. In addition, 50% FC increased the stomatal length/width ratio and their size though no effect in stomatal density was found. Thus, Copaifera langsdorffii grows and thrives in mining tailings even under reduced water availability up to 50% FC showing potential for reforestation systems.

The effect of plantain peel biochar on the uptake of six heavy metals (Cd, Cr, Cu, Fe, Pb and Zn) in spinach (Spinacia oleracea L.) irrigated with untreated wastewater was investigated in nine outdoor lysimeters (0.45 m diameter × 1.0 m height) arranged in a completely randomised design with three replicates. The lysimeters were packed with sandy soil (bulk density 1.35 Mg m⁻³) and brought to field capacity 1 day before starting the experiment. Biochar (1% w/w) was mixed in the top 0.10 m of soil under biochar amendment. Spinach were planted in each lysimeter, irrigated (every 10 days for 4 times in total), harvested (harvest 1 and harvest 2) and analysed for the heavy metals. Spinach leaves accumulated more heavy metals than the roots and stems. Biochar amendment did not affect the translocation of heavy metals (Cd, Cu, Cr, Fe and Pb) to spinach leaves, possibly due to competition with other compounds in the soil solution. However, the biochar amendment improved CEC and increased the pH of soils which resulted in a 42% reduction of translocation of Zn in spinach leaves. Assuming daily spinach consumption of 200 g per person, Zn in spinach grown in soil amended with biochar would be below the provisional maximum tolerable daily intake limit for adults (20 mg) as prescribed by WHO/FAO/IAEA. Consumption of spinach grown with wastewater in soil without biochar amendment may not be safe because of Zn toxicity. Likewise, the concentration of Cd, above CODEX permissible levels in the spinach leaves and eleven times higher in wastewater than freshwater irrigation, raises a concern for consumers in developing countries where untreated wastewater is often used for irrigation.

Potassium silicate drilling fluid (PSDF) is a relatively new type of drilling waste generated by the oil and gas industry. PSDF effects on soil, vegetation, and ground water must be determined before its land disposal and use in reclamation can be regulated. A laboratory column leachate study was conducted to quantify the response of select soil and leachate properties to PSDF at various depths in soil column profiles. A spent PSDF was applied to two soils (sand and loam textures) at four rates (20, 40, 60, 120 m³ ha⁻¹) with two application methods (incorporated, sprayed). Changes to soil and leachate properties were at values that would not be detrimental to most plant species when PSDF was applied at ≤60 m³ ha⁻¹. Applying PSDF at 120 m³ ha⁻¹had significant effects on soil properties and leachate quality. Hydraulic conductivity and field capacity were significantly reduced, and soil available potassium and sulfate concentrations, pH, and salinity increased with PSDF. Incorporated PSDF in the upper 10 cm of soil accelerated PSDF element transport through soil columns to leachate and increased organic carbon and salinity in leachate. PSDF application rate significantly reduced soil field capacity, available nitrogen, and increased salinity at the highest rates in loam soil, suggesting a threshold beyond which conditions will not be suitable for land spraying PSDF. This research demonstrates that PSDF has potential to improve soil short term water availability, macronutrient potassium and sulfur for disposal on cultivated and uncultivated lands. This potential should be field tested.

The effects of urea, (NH₄)₂SO₄, KNO₃, and NH₄NO₃ on nitrous oxide (N₂O) emission from soil at field capacity and submerged condition were studied during 120 days in the laboratory. Soils in both moisture regimes gave higher emissions in the beginning, which were reduced later. Total emission of N₂O was higher at submergence as compared to field capacity regardless of fertilizer type. At field capacity soil fertilized with ureaemitted the highest amount of N₂O (1903 μg N₂O-N kg⁻¹ soil) during 120 days while at submerged condition, soil with NH₄NO₃ gave the highest emission (4843 μg N₂O-N kg⁻¹ soil). In another study, the efficacy of seven nitrification inhibitors in reducing the emission of N₂O-N from soil fertilized with urea was tested in the laboratory. Nitrapyrin, 2-amino-4-chloro-6-methylpyrimidine (AM), and dicyandiamide (DCD) reduced the emission to 12, 24, and 63% that of urea, respectively, whereas sodium thiosulphate, sulphur, acetylene,and thiourea had no effect on emission of N₂O. In submerged conditions none of the inhibitors reduced the emission.

Processes contributing to acid release/consumption during weathering of a lignite mine spoil (2.3% w/w S as sulfides) from As Pontes (N.W. Spain) were studied under three moisture conditions (at field capacity or under alternate wetting-drying or forced percolation), which were simulated in laboratory experiments. Oxidation of sulfides to sulfates was favoured under all three moisture conditions, releasing most acid in spoil kept at field capacity. Hydroxysulfates formed in spoil kept at field capacity or under alternate wetting-drying conditions, thereby contributing to acid release. Acid consumption by dissolution of clay minerals, especially micas, was favoured under all three moisture conditions, but was particularly intense in spoil at field capacity. Dissolution of aluminium oxides was also favoured under all the moisture conditions studied.

Due to the repeated applications of pesticides, the amount of the pesticides and their products by decomposition may accumulate in the soil ecosystems which are affected by abiotic and biotic factors. Soil microorganisms are the important players that are able to decompose and utilize these chemical waste materials as energy sources and regulate them in the cycling in the soil environment. One of the important insecticides for the control of insect pests including aphids is spirotetramat which can provide protection to plant roots from the attack of insects when it was sprayed on the crops. However, effects of high concentrations of spirotetramat on soil microbial respiration under different soil water contents are unknown. Recommended field dose (RFD) and its 5 (RFD × 5) and 10 (RFD × 10) folds of spirotetramat were mixed with a clay soil; these mixtures were humidified at 50% (50FC), 75% (75FC), and 100% (100FC) of field capacity and then incubated at 28 °C for 21 days. At the end of the incubation period, (1) in general, soil microbial respiration was significantly increased as soil moisture increased in all treatments (50FC < 75FC < 100FC, P < 0.05); (2) all concentrations of spirotetramat significantly decreased the microbial respiration under 100FC (P < 0.05); (3) only RFD × 5 significantly reduced this activity under 75FC (P < 0.05); (4) no significant differences between control and treatments were found under 50FC. In conclusion, high concentrations of spirotetramat insecticide had low toxic effects on soil microbial respiration while the soil moisture regulated the toxicity effects of this insecticide.

Herbicide imazamox is widely applied in the 4–8 leaf period after sowing of sunflower cultivation in Turkey. The recommended dose [(RDX1), 480 g active ingredient l⁻¹], and doses three and five times (RDX3, and RDX5) of this herbicide were added to the sunflower soil without imazamox (UI) and sunflower soil previously applied with imazamox (AI) from a district of Osmaniye (Turkey) under Mediterranean climate conditions in order to determine their effects on soil microbial activity as measured by carbon and nitrogen mineralization (42 days) at the constant incubation conditions (28 ºC and moistened at 80% of the field capacity). Cumulative carbon mineralization [mg C(CO₂)/100 g] in both UI and AI soils increased depending on the incubation time during the 42 days. This result might explain that herbicide added to the soil at different dosages increased the microorganism respiration because of its use as a carbon source. The nitrogen mineralization rate was high in AI soils than in UI soils. There was statistical difference found between control soils and both RDX1 and RDX3 of AI soils (P < 0.001). It is possible to conclude that the presence of imazamox inhibited the soil microorganisms responsible for nitrogen mineralization.

The removal of total petroleum hydrocarbons (TPHs) from contaminated mining soil was carried out under in vitro conditions. The aerobic consumption of TPH in the slow bioremediation stage via biostimulation with native microorganisms and biostimulation-bioaugmentation with autochthonous fungal isolates was evaluated. The initial TPH concentration was 70,880 ± 975 mg TPH/kg soil, soil was amended with nutrients at a C:N:P ratio of 100:15:1, the water content was adjusted to the soil field capacity, and batch microcosm reactors were incubated at room temperature (20.5 ± 3.1°C) for 90 days. The bioaugmentation process was tested using four hydrocarbonoclastic fungal strains isolated from the same contaminated mining soil individually and a mixed culture of the four isolates. The molecular characterization of the isolated fungi was based on sequence analysis of 18S rRNA, and the fungi were identified as Aspergillus niger MT786339.1, Aspergillus fumigatus MT786338.1, Aspergillus terreus MT786341.1, and Aspergillus flavus MT786340.1. The best TPH removal was achieved by inoculation with the fungal consortium (57 ± 1.97%) at 45 days (slow stage) after initiating the biostimulation process, followed by inoculation with Aspergillus niger (49 ± 1.2%), Aspergillus terreus (44 ± 0.67%), Aspergillus fumigatus (35 ± 0.98%), and Aspergillus flavus (32 ± 0.38%), while the degradation rate achieved with native microorganisms was only 21.6 ± 1.5%; statistical analysis of the results showed significant differences.

Pistacia atlantica Desf. (Beneh) is an important woody species that has been facing significant challenges to its natural regeneration and reforestation in Iran. This study investigates the interaction of soil moisture and shade on growth, chemical contents, and morphological and physiological characteristics of Beneh saplings. One-year-old Beneh saplings were treated with varying amounts of soil moisture (20, 50, and 100% of field capacity) and shade (0, 30, and 50% of full sunlight) in a split-plot experiment of a randomized complete block design in semiarid conditions of the Alborz Research Station of the Research Institute of Forests and Rangelands (RIFR) in Iran. The results indicate that soil moisture significantly affects the water content of the leaf, total chlorophyll, proline content, activity of catalase enzyme, leaf dry biomass, leaflet area, and dry stem biomass in the leaf. Shade significantly affected total chlorophyll, catalase enzyme activity, specific leaflet area, relative water content of the leaf, proline content, dry root biomass, and leaflet area. The interaction of shade and soil moisture significantly affected seedling height, catalase enzyme activity, specific leaflet area, and nitrogen and potassium content of the leaf. Shade moderates the stress of drought on Beneh saplings, but shading of Beneh saplings is not recommended in conditions where there is no concern about soil moisture. These conclusions can be used to improve the production of Beneh saplings in nurseries.