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Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma
2022
Cormier, Bettie | Cachot, Jérôme | Blanc, Mélanie | Cabar, Mathieu | Clérandeau, Christelle | Dubocq, Florian | Le Bihanic, Florane | Morin, Bénédicte | Zapata, Sarah | Bégout, Marie-Laure | Cousin, Xavier | Environnements et Paléoenvironnements OCéaniques (EPOC) ; Observatoire aquitain des sciences de l'univers (OASU) ; Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) | Örebro University | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Laboratoire Service d' Experimentations Aquacoles [Palavas les Flots] (LSEA MARBEC) ; MARine Biodiversity Exploitation and Conservation [Palavas] (UMR MARBEC PALAVAS) ; Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) | This work was developed under the EPHEMARE project (Ecotoxicological effects of microplastics in marine ecosystems), supported by national funding agencies within the framework of JPI Oceans (FCT JPIOCEANS/0005/2015; FORMAS, 2015-01865; ANR-15-JOCE-0002-01). Bettie Cormier was directly supported by an IdEx grant from the University of Bordeaux. | ANR-15-JOCE-0002,EPHEMARE,Ecotoxicological effects of microplastics in marine ecosystems(2015)
International audience | Microplastics (MPs), widely present in aquatic ecosystems, can be ingested by numerous organisms, but their toxicity remains poorly understood. Toxicity of environmental MPs from 2 beaches located on the Guadeloupe archipelago, Marie Galante (MG) and Petit-Bourg (PB) located near the North Atlantic gyre, was evaluated. A first experiment consisted in exposing early life stages of zebrafish (Danio rerio) to MPs at 1 or 10 mg/L. The exposure of early life stages to particles in water induced no toxic effects except a decrease in larval swimming activity for both MPs exposures (MG or PB). Then, a second experiment was performed as a chronic feeding exposure over 4 months, using a freshwater fish species, zebrafish, and a marine fish species, marine medaka (Oryzias melastigma). Fish were fed with food supplemented with environmentally relevant concentrations (1% wet weight of MPs in food) of environmental MPs from both sites. Chronic feeding exposure led to growth alterations in both species exposed to either MG or PB MPs but were more pronounced in marine medaka. Ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities were only altered for marine medaka. Reproductive outputs were modified following PB exposure with a 70 and 42% decrease for zebrafish and marine medaka, respectively. Offspring of both species (F1 generation) were reared to evaluate toxicity following parental exposure on unexposed larvae. For zebrafish offspring, it revealed premature mortality after parental MG exposure and parental PB exposure produced behavioural disruptions with hyperactivity of F1 unexposed larvae. This was not observed in marine medaka offspring. This study highlights the ecotoxicological consequences of short and long-term exposures to environmental microplastics relevant to coastal marine areas, which represent essential habitats for a wide range of aquatic organisms.
Show more [+] Less [-]Prediction of the PCDD/F and dl-PCB 2005-WHO-TEQ content based on the contribution of six congeners: Toward a new screening approach for fish samples?
2010
Cariou, Ronan | Marchand, Philippe | Venisseau, Anais | Brosseaud, Aline | Bertrand, Dominique, D. | Qannari, El Mostafa | Antignac, Jean-Philippe | Le Bizec, Bruno | École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS) | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de la Recherche Agronomique (INRA) | Unité de Sensométrie et Chimiométrie ; Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA) | Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | Current European Union regulation regarding polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) in food and feed is based on Toxic Equivalent Quotient (TEQ) concept. For confirmatory purpose, the isotope-dilution method associated to a measurement by gas chromatography coupled with high resolution mass spectrometry is usually the method of choice for precisely measuring the 29 target congeners in three separated fractions. Time and cost related to these analyses are very significant. Various kinds of screening concepts can be considered. In the present study, we elaborated and validated a prediction model for the 2005 World Health Organization TEQ in fish, based on the measurement of 4 PCDD/F and 2 non-ortho dl-PCB congeners, potentially analyzable in a single extracted fraction by gas chromatography coupled with mass spectrometry. Large independent datasets have been used for model elaboration (n = 108) and validation (n = 363, n = 357 and n = 6).
Show more [+] Less [-]Correlations between dioxin-like and indicators PCBs: Potential consequences for environmental studies involving fish or sediment
2009
Babut, M. | Miege, Cecile | Villeneuve, B. | Abarnou, A. | Duchemin, J. | Marchand, P. | Narbonne, J.F. | Biologie des écosystèmes aquatiques (UR BELY) ; Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) | Qualité des eaux et prévention des pollutions (UR QELY) ; Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) | Physiologie Fonctionnelle des Organismes Marins (PFOM) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) | Agence de l'Eau Seine-Normandie | École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS) | Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC) ; Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA | Among the numerous PCB congeners, most of the dioxin-like PCBs (DL-PCBs) need to be characterized by hyphenated techniques. It has been shown in several instances that these congeners are well related to the total PCB content in fish.We examined datasets collected mainly in France, on freshwater and marine fish and sediments. A statistical model linking DL- and indicator PCBs was developed for a dataset composed of freshwater fishes, and proved to predict well DL-PCBs from indicator PCBs in all other fish sets, including marine ones. Type II error rates remained low in almost all fish sets. A similar correlation was observed in sediments. Non-dioxin-like PCBs elicit various adverse effects and represent 95% of the total PCBs. A European guideline for them is needed; the correlation between DL- and indicator PCBs could help develop this standard in the future.
Show more [+] Less [-]Persistent organic pollutants in benthic and pelagic organisms off Adélie Land, Antarctica.
2013
Goutte, Aurélie | Chevreuil, M. | Alliot, F. | Chastel, Olivier | Cherel, Yves | Eléaume, Marc | Massé, Guillaume | Centre d'Études Biologiques de Chizé (CEBC) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN) ; Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)) ; École normale supérieure - Paris (ENS-PSL) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Hydrologie Environnement ; Université Pierre et Marie Curie - Paris 6 (UPMC) | Département Milieux et Peuplements Aquatiques ; Muséum national d'Histoire naturelle (MNHN)
International audience | The concentrations of polychlorinated biphenyls (PCB), hexachlorobenzene (HCB), pentachlorobenzene (PeCB) and polybrominated diphenylethers (PBDE) were described in benthic and pelagic species collected off Adélie Land, Antarctica. Strong differences were observed among species, with reduced PeCB and HCB levels in benthic species, and elevated PCB levels in the Antarctic yellowbelly rockcod, the Antarctic sea urchin and the snow petrel. Lower-chlorinated congeners were predominant in krill; penta-PCBs in benthic organisms; hexa- and hepta-PCBs in seabirds and cryopelagic fish. This segregation may result from sedimentation process, specific accumulation and excretion, and/or biotransformation processes. The presence of PBDEs in Antarctic coastal organisms may originate from atmospheric transport and partly from a contamination by local sources. Although POP levels in Antarctic marine organisms were substantially lower than in Arctic and temperate organisms, very little is known about their toxic effects on these cold-adapted species, with high degree of endemism.
Show more [+] Less [-]Persistent organic pollutants in benthic and pelagic organisms off Adélie Land, Antarctica.
2013
Goutte, Aurélie | Chevreuil, M. | Alliot, Fabrice | Chastel, Olivier | Cherel, Yves | Eléaume, Marc | Massé, Guillaume | Centre d'Études Biologiques de Chizé (CEBC) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN) ; Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)) ; École normale supérieure - Paris (ENS-PSL) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-École polytechnique (X) ; Institut Polytechnique de Paris (IP Paris)-Institut Polytechnique de Paris (IP Paris)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-École polytechnique (X) ; Institut Polytechnique de Paris (IP Paris)-Institut Polytechnique de Paris (IP Paris)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Hydrologie Environnement ; Université Pierre et Marie Curie - Paris 6 (UPMC) | Département Milieux et Peuplements Aquatiques ; Muséum national d'Histoire naturelle (MNHN)
International audience | The concentrations of polychlorinated biphenyls (PCB), hexachlorobenzene (HCB), pentachlorobenzene (PeCB) and polybrominated diphenylethers (PBDE) were described in benthic and pelagic species collected off Adélie Land, Antarctica. Strong differences were observed among species, with reduced PeCB and HCB levels in benthic species, and elevated PCB levels in the Antarctic yellowbelly rockcod, the Antarctic sea urchin and the snow petrel. Lower-chlorinated congeners were predominant in krill; penta-PCBs in benthic organisms; hexa- and hepta-PCBs in seabirds and cryopelagic fish. This segregation may result from sedimentation process, specific accumulation and excretion, and/or biotransformation processes. The presence of PBDEs in Antarctic coastal organisms may originate from atmospheric transport and partly from a contamination by local sources. Although POP levels in Antarctic marine organisms were substantially lower than in Arctic and temperate organisms, very little is known about their toxic effects on these cold-adapted species, with high degree of endemism.
Show more [+] Less [-]First Assessment of Rare Earth Element Organotropism in Solea Solea in a Coastal Area: The West Gironde Mud Patch (France)
2023
Labassa, Maëva | Pereto, Clément | Schäfer, Jörg | Hani, Younes M.i. | Baudrimont, Magalie | Bossy, Cécile | Dassié, Émilie P. | Mauffret, Aourell | Deflandre, Bruno | Gremare, Antoine | Coynel, Alexandra
Few studies exist on bioaccumulation and internal distribution of Rare Earth Elements (REEs) in marine fishes. REEs organotropism was determined in common sole (Solea solea) from the West Gironde Mud Patch (WGMP; N-E Atlantic Coast, France). The highest REEs concentrations occurred in liver (213 ± 49.8 µg kg-1 DW) and gills (119 ± 77.5 µg kg-1 DW) followed by kidneys (57.7 ± 25.5 µg kg-1 DW), whereas the lowest levels were in muscles (4.38 ± 1.20 µg kg-1 DW) of Solea solea. No significant age- or sex-related differences were observed. The organotropism varied among groups of REEs. Light and heavy REEs preferentially accumulated in liver and gills, respectively. All considered organs showed different normalized REEs patterns, suggesting differences in internal distribution processes between organs. Further work should address: (1) baseline levels worldwide, and (2) factors controlling uptake and organ-specific bioaccumulation of REEs.
Show more [+] Less [-]Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in D. rerio and Oryzias melastigma
2022
Cormier, Bettie | Cachot, Jerome | Blanc, Melanie | Cabar, Mathieu | Clérandeau, Christelle | Dubocq, Florian | Le Bihanic, Florane | Morin, Bénédicte | Zapata, Sarah | Bégout, Marie-laure | Cousin, Xavier
Microplastics (MPs), widely present in aquatic ecosystems, can be ingested by numerous organisms, but their toxicity remains poorly understood. Toxicity of environmental MPs from 2 beaches located on the Guadeloupe archipelago, Marie Galante (MG) and Petit-Bourg (PB) located near the North Atlantic gyre, was evaluated. A first experiment consisted in exposing early life stages of zebrafish (Danio rerio) to MPs at 1 or 10 mg/L. The exposure of early life stages to particles in water induced no toxic effects except a decrease in larval swimming activity for both MPs exposures (MG or PB). Then, a second experiment was performed as a chronic feeding exposure over 4 months, using a freshwater fish species, zebrafish, and a marine fish species, marine medaka (Oryzias melastigma). Fish were fed with food supplemented with environmentally relevant concentrations (1% wet weight of MPs in food) of environmental MPs from both sites. Chronic feeding exposure led to growth alterations in both species exposed to either MG or PB MPs but were more pronounced in marine medaka. Ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities were only altered for marine medaka. Reproductive outputs were modified following PB exposure with a 70 and 42% decrease for zebrafish and marine medaka, respectively. Offspring of both species (F1 generation) were reared to evaluate toxicity following parental exposure on unexposed larvae. For zebrafish offspring, it revealed premature mortality after parental MG exposure and parental PB exposure produced behavioural disruptions with hyperactivity of F1 unexposed larvae. This was not observed in marine medaka offspring. This study highlights the ecotoxicological consequences of short and long-term exposures to environmental microplastics relevant to coastal marine areas, which represent essential habitats for a wide range of aquatic organisms.
Show more [+] Less [-]Exposure of zebrafish to an environmental mixture of persistent organic pollutants triggers an increase in anxiety-like syndrome but does not affect boldness in unexposed offspring
2023
Alfonso, Sébastien | Blanc, Mélanie | Cousin, Xavier | Bégout, Marie-Laure | MARine Biodiversity Exploitation and Conservation [Sète] (UMR MARBEC SETE) ; Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | COISPA Technologia y Ricerca ; Partenaires INRAE
International audience | Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants (POPs) that are present as complex mixtures in all environmental compartments, including aquatic ecosystems. However, little is known about the effects of such complex mixtures on teleost behaviour. In this study, zebrafish (Danio rerio) were chronically exposed to an environmentally relevant mixture (MIX) containing 22 PCB and 7 PBDE congeners through diet from 5 days post fertilization onwards. MIX-exposed F0 fish produced offspring (F1 and F2 generations) that were fed using plain food and grown until adulthood. In each generation, five behavioural traits (i.e. boldness, activity, sociality, exploration and anxiety) were evaluated by the mean of different experimental set-ups. Two distinct behavioural syndromes were identified: boldness, positively correlated to activity and exploration; and anxiety, associated with low sociality. F0 fish did not display any behavioural disruption resulting from POP exposure whereas F1 MIX fish were bolder than fish from other generations but did not differ significantly from F1 controls. F2 MIX fish displayed a higher anxiety syndrome than F2 controls. This is of particular importance since such behavioural changes in offspring generations may have persistent ecological consequences, may affect fitness and hence cause detrimental effects on wild fish populations exposed to POP mixtures.
Show more [+] Less [-]Bioaccumulation of trace elements in pelagic fish from the Western Indian Ocean
2007
Kojadinovic, Jessica | Potier, Michel | Le Corre, Matthieu | Cosson, Richard P. | Bustamante, Paco
Trace elements were analyzed in fish of commercial interest to determine their importance in marine systems of the Western Indian Ocean and their bioaccumulation patterns. The results are equivalent or lower than levels reported in ichthyofauna worldwide. Certain values of muscular Cd, Hg, Pb and Zn were, however, above thresholds for human consumption. Levels varied among tissues, species and fish length, but were seldom influenced by the nutritional condition of the fish, its gender and its reproductive status. Correlations between hepatic Hg and Se levels in Swordfish (r(2) = 0.747) and Yellowfin Tunas (r(2) = 0.226), and among metallothionein linking metals imply the existence of detoxification processes in these species. Level differences between fish from the Mozambique Channel and Reunion Island reflect differences of diets rather than differences of elemental availability in both environments. (c) 2006 Elsevier Ltd. All rights reserved.
Show more [+] Less [-]A large diversity of organohalogen contaminants reach the meso- and bathypelagic organisms in the Bay of Biscay (northeast Atlantic)
2022
Munschy, Catherine | Spitz, J. | Bely, Nadege | Héas-moisan, Karine | Olivier, Nathalie | Pollono, Charles | Chouvelon, Tiphaine
Deep-sea ecosystems play a key role in the cycling and vertical transfer of matter and energy in oceans. Although the contamination of deep-sea demersal and benthic organisms by persistent organic pollutants has been proven, deep pelagic species have been far less studied. To fill these gaps, we studied the occurrence of a large variety of hydrophobic organic contaminants including polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), legacy and alternative brominated flame retardants (BFRs) and per- and polyfluoroalkyl substances (PFASs) in crustaceans and fish species collected in the Bay of Biscay, northeast Atlantic. The results highlighted the global predominance of PCBs in fish, followed by OCPs, PFASs and PBDEs, with highly variable concentrations among species. Most of the chlorinated or brominated contaminants showed increasing concentrations with increasing δ15N values, while most PFASs showed inverse trends. The contaminant profiles and diagnostic ratios revealed species-specific metabolic capacities and peculiar contribution of highly-brominated BFRs.
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