The present investigation aims to study the comparative effect of different treatments of organic and inorganic fertilizers on the growth and biochemical constituents of groundnut (Arachis hypogaea L.). The plants were raised in the field with different treatments of fertilizers like arbuscular mycorrhizal fungi (AMF), Azospirillum (AZM), market waste compost (MWC), nitrogen (N), phosphorus (P) and potassium (K). The plant samples were collected after 15, 30, 60 and 90 days for the measurement of different growth parameters. The analysis of the morphological and biochemical parameters such as shoot and root length, fresh and dry weight, total leaf area, chlorophyll ?a? and ?b?, total chlorophyll, amino acids, starch and total sugar was made at different days intervals. The effect of organic and inorganic fertilizers on growth parameters and biochemical constituents of leaves was significantly increased in all the treatments. In general, bio-fertilizer applications are to boost the effectiveness of Arbuscular Mycorrhizal Fungi (AMF), Azospirillum (AZM) and market waste compost (MWC) on nodulation and yield of groundnut. The organic fertilizers treated plants showed better performance than the inorganic fertilizers and control.