Heavy metal poisoning means an excess of the required concentration that is found naturally on earth. In the present experiment, it has been observed that the natural water source surrounded by mountains is also polluted with arsenic because these heavy metals like arsenic are naturally contained in rocks that accumulate in the water source with soil erosion and rain. In this experiment arsenic was found in the water of Chhilpura pond, in which the haematology and biochemistry of catfish (Clarias batrachus) were studied, it was found that the RBC, haemoglobin, PCV, MCV, MCH, platelets, glucose and protein contents of catfish were found to be relatively low as compared to the control, whereas the WBC was observed to be higher to control and differential leucocyte count fluctuations were observed. It can be inferred from this experiment that heavy metals such as arsenic alter the haematology and biochemistry of catfish (Clarias batrachus).

The acute toxicity bioassay of textile industrial effluent (TIE) was carried out by the probit analysis method. The 12, 24, 48, 72 and 96 h LC50 values were obtained as 56.23 %, 28.84 %, 22.38% and 16.59% respectively. LC50 values were significantly decreased with increase in effluent concentration. The safe value was found to be 32.88 %. The experimental fish C.batrachus exhibited stressful behaviour which increased with toxicant concentration. Due to acute toxicity of textile based industrial effluent, experimental fish C. batrachus expressed high secretion of mucus, uncoordinated and tailfin movement, surfacing, loss of buoyancy, escaping tendency, hyperactivity, and discolouration of the skin. Mortalities were observed in all treatments except control. The haematological analysis was also carried out in experimental fish C.batrachus exposed to various periods in textile-dyeing effluents. Haematological data were evaluated for parameters such as Hb, RBCs, WBCs, PCV, MCH, and MCHC of the test species. The alterations of these parameters have been discussed. So it can be concluded that the TIE is toxic to fishes and aquatic organisms.