Foliage of three trembling aspen clones differing in O3 tolerance from Rhinelander, Kenosha and Kalamazoo were examined for 24 elements in the year 2001 and they were analyzed by INAA at reactor IBR-2, by AAS Varian 400 and by elemental analyzer LECO SC 132 and SP 228. In the fofliage of trembling aspen we found no statistically significant difference in the concentration of 22 elements except for K and Ni between clones. For the concentrations of elements between localities we found statistically significant difference for Al, Ba, Ca, Cd, Cl, Co, Cu, La, Mo, Na, Ni, Pb, Sm, Sr and Zn

We studied the effects of elevated CO2 and elevated temperature on photosynthesis and composition of Scots pine needles during three years of treatments, started in August 1996. CO2 and temperature were elevated day- and year- round in closed-top chambers, which were constructed around naturally regenerated Scots pine trees growing at a nitrogen poor site. Our aim was to study, whether there is photosynthetic acclimation during three years of growth at elevated CO2 and elevated temperature in Scots pine, and whether acclimation response is connected to the aging of the needles

We aim to differentiate the various visible leaf symptoms in the field survey with microscopical methods, and to better know, how the leaf, tissue and cell response differs between injury from ozone, heavy metals (both oxidative stress) and biotic infections

The biological effects of both elevated CO2 and N deposition on model ecosystem were investigated in the Birmensdorf open-top chamber facility. Each of the 16 chambers was divided into two compartments with a ground area of 3 msub2 and filled with natural unfertilized forest soils from two sites (one acidic, the other calcareous). Elevated CO2 significantly increased O and Zn concentrations in beech leaves and those of Zn in spruce needles on the calcareous soils. Enhanced N deposition also led to a dilution of nutrients and increased N contents

Emissions, especially of SO2 and to a certain degree of NOx have been reduced markedly in Slovakia during the 90s. Problems related to climate change and especially ozone are increasingly growing. The aim was to evaluate the long-term air pollution effect on forest ecosystems in mountain area with prevailing distribution of spruce through evaluation of spruce needle surface structure in relation to mineral nutrient status of trees. According to strong relationship between the coefficient of epicuticular wax degradation (Q) and accumulation of S and other elements it can be suggested that evaluation of needle surface structure by means of Q well reflects the surface status of needles

Two holm oak (Quercus ilex L.) sites in Tuscany, Central Italy, were studied in July 2000. Leaf morphological characteristics (area, dry weight, specific dry weight, water content, epicuticular wax amount, stomatal density) and leaf fluorescence were measured. Ozone flux into the leaves was calculated on a stomatal conductance basis. Among the measured parameters, only stomatal density and wax amount significantly differed between the sites. This response might indicate an adaptation to air pollutants, of which tropospheric ozone might be of the greatest importance

The results showed that ozone specific symptoms can be detected in the needles of mature spruces in the southern Sweden with AOT40 considerably lower than the present critical level of 10 000 nl 1sup-1. The results could be expressed as an index per tree and needle generations, but also as a single value for forest stand allowing statistical tests. The results from this study support the general hypothesis and results from exposure and field studies that low nutrient concentrations or nutrient deficiency modifies the ozone response in plants. The results also show that together with low nutrition status ozone may affect the hardiness status of the needles

Needle retention, needle density, height and radial increments were surveyed on Scots pine (Pinus sylvestris L.) in four forest regions, In the each forest region research was conducted on seven about 45-year-old pines. The Needle Trace Method (NTM) was used to determine needle retention and needle density along the main stem retrospectively for the last four decades. The NTM invented by Kurkela and Jalkanen is based on the examination of the vascular bundles connecting the needles and shoot pith. There are presented the long-term average values of needle retention in the particular forest regions, their differences. Needle retention and needle density reflected air pollution stress on the pines

Both CO2 and ozone increased the diseased leaf area of clone V5952 in Exp. 1 in the year 2000. The size of spots increased most under ozone fumigation, and the number of spots under ozone and CO2 + O3 fumigations. In clone K1659 all fumigation treatments decreased or had no effect on the DLA, or the number and size of the leaf spots. Also the number of fallen leaves under fumigation treatments was higher in clone V5952 than in clone K1659. Analysis of the year 2001 monitoring results is currently going on

Significant correlations (p0.05) were found between the concentration of elements in the c needles and the distance of the sites from nitrogen ferilizer factory (NFF): the amounts of C, K, Mg, Zn, and Rb in the c+1 needles were increasing, whereas concentrations of N, P, Al, Mn, Cu, Ni, Sr and Cd were decreasing alongside the transect from NFF. Statistical analyses performed on logarithms and estimation of components of variance by maximum likelihood has shown that the age of the needles and the sites were the most important, while year of sampling was much smaller source of variance. Only a few significant differences were found comparing the amount of elements in Scots pine needles collected in four-year space