The current study investigates the dissipation kinetics of two imidacloprid (IMI) nanoformulations (entitled: Nano-IMI and Nano-IMI/TiO2) on common bean (Phaseolus vulgaris) seeds under field conditions and compares them with 35% Suspension Concentrate (SC) commercial formulation. To do so, it sprays P. vulgaris plants at 30 and 60 g/ha within green bean stage, sampling them during the 14-day period after the treatment. Following extraction and quantification of IMI residues, dissipation data have been fitted to simple-first order kinetic model (SFOK) and to first-order double-exponential decay (FODED) models, with 50% and 90% dissipation times (DT50 and DT90, respectively) assessed along the pre-harvest interval (PHI). With the exception of Nano-IMI at 60 g/ha, other decline curves are best fitted to the FODED model. In general, dissipation is faster for Nano-IMI (at 30 g/ha: DT50 = 1.09 days, DT90 = 4.30 days, PHI = 1.23 days; at 60 g/ha: DT50 = 1.29 days, DT90 = 4.29 days, PHI = 2.95 days) and Nano-IMI/TiO2 (at 30 g/ha: DT50 = 1.15 days, DT90 = 4.40 days, PHI = 1.08 days; at 60 g/ha: DT50 = 0.86 days, DT90 = 4.92 days, PHI = 3.02 days), compared to 35% SC (at 30 g/ha: DT50 = 1.58, DT90 = 6.45, PHI = 1.93; at 60 g/ha: DT50 = 1.58 days, DT90 = 14.50 days, PHI = 5.37 days). These results suggest the suitability of Nano-IMI and Nano-IMI/TiO2 application at both rates in terms of their residues on P. vulgaris seeds.

International audience | There is currently a lack of ecotoxicity tests adapted to earthworm species of higher ecological relevance and whose endpoints could be directly related to their ecological role in the soil. We propose a new and relatively simple ecotoxicity test based on the estimation of cast production (CP) by Lumbricus terrestris under laboratory conditions. CP was found to be linearly correlated to earthworm biomass and to be greatly influenced by soil water content. Azinphos-methyl had no effect on CP at all the concentrations tested. Significant decreases were observed at the normal application rate for other pesticides with (imidacloprid, carbaryl, methomyl) or without (ethyl-parathion and chlorpyrifos-ethyl) a clear concentration–effect response. For the highest concentration tested, reduction in CP varied between 35 and 67%. CP is straightforward and rapidly measured and ecologically meaningful. We thus believe it to be of great use as an endpoint in ecotoxicity testing.

Due to technical limitations, there have been minimal studies performed on thermal preferences and thermotactic behaviors of aquatic ectotherm species commonly used in ecotoxicity testing. In this work, we demonstrate an innovative, purpose-built and miniaturized electrothermal array for rapid thermal preference behavioral tests. We applied the novel platform to define thermal preferences in multiple invertebrate and vertebrate species. Specifically, Dugesia notogaea (freshwater planarians), Chironomus tepperi (nonbiting midge larvae), Ostracoda (seed shrimp), Artemia franciscana (brine shrimp), Daphnia carinata (water flea), Austrochiltonia subtenuis (freshwater amphipod), Physa acuta (freshwater snail), Potamopyrgus antipodarum (New Zealand mud snail) and larval stage of Danio rerio (zebrafish) were tested. The Australian freshwater water fleas, amphipods, snail Physa acuta as well as zebrafish exhibited the most consistent preference to cool zones and clear avoidance of zones >27 °C out of nine species tested. Our results indicate the larval stage of zebrafish as the most responsive species highly suitable for prospective development of multidimensional behavioral test batteries. We also showcase preliminary data that environmentally relevant concentrations of pharmaceutical pollutants such as non-steroidal anti-inflammatory drug (NSAID) ibuprofen (9800 ng/L) and insecticide imidacloprid (4600 ng/L) but not anti-depressant venlafaxine (2200 ng/L) and (iv) anticonvulsant medications gabapentin (400 ng/L) can perturb thermal preference behavior of larval zebrafish. Collectively our results demonstrate the utility of simple and inexpensive thermoelectric technology in rapid exploration of thermal preference in diverse species of aquatic animals. We postulate that more broadly such technologies can also have added value in ecotoxicity testing of emerging contaminants.

Freshwater ecosystems are recognized as non-negligible sources of plastic contamination for the marine environment that is the final acceptor of 53 thousand tons of plastic per year. In this context, microplastic particles are well known to directly pose a great threat to freshwater organisms, they also indirectly affect the aquatic ecosystem by adsorbing and acting as a vector for the transport of other pollutants (“Trojan horse effect”). Polystyrene is one of the most widely produced plastics on a global scale, and it is among the most abundant microplastic particles found in freshwaters. Nevertheless, to date few studies have focused on the eco-genotoxic effects on freshwater organisms caused by polystyrene microplastic particles (PS-MPs) in combination with other pollutants such as pharmaceuticals and pesticides. The aim of this study is to investigate chronic and sub-chronic effects of the microplastic polystyrene beads (PS-MP, 1.0 μm) both as individual xenobiotic and in combination (binary/ternary mixtures) with the acicloguanosine antiviral drug acyclovir (AC), and the neonicotinoid broad-spectrum insecticide imidacloprid (IMD) in one of the most sensitive non-target organisms of the freshwater food chain: the cladoceran crustacean Ceriodaphnia dubia. Considering that the individually selected xenobiotics have different modes of action and/or different biological sites, the Bliss independence was used as reference model for this research. Basically, when C. dubia neonates were exposed for 24 h to the mixtures during Comet assay, mostly an antagonistic genotoxic effect was observed. When neonates were exposed to the mixtures for 7 days, mostly an additive chronic toxic effect occurred at concentrations very close or even overlapping to the environmental ones ranging from units to tens of ng/L for PS-MPs, from tenths/hundredths to units of μg/L for AC and from units to hundreds of μg/L for IMD, revealing great environmental concern.

Aquatic pollution from emerging organic contaminants (EOCs) is of key environmental importance in India and globally, particularly due to concerns of antimicrobial resistance, ecotoxicity and drinking water supply vulnerability. Here, using a broad screening approach, we characterize the composition and distribution of EOCs in groundwater in the Gangetic Plain around Patna (Bihar), as an exemplar of a rapidly developing urban area in northern India. A total of 73 EOCs were detected in 51 samples, typically at ng.L⁻¹ to low μg.L⁻¹ concentrations, relating to medical and veterinary, agrochemical, industrial and lifestyle usage. Concentrations were often dominated by the lifestyle chemical and artificial sweetener sucralose. Seventeen identified EOCs are flagged as priority compounds by the European Commission, World Health Organisation and/or World Organisation for Animal Health: namely, herbicides diuron and atrazine; insecticides imidacloprid, thiamethoxam, clothianidin and acetamiprid; the surfactant perfluorooctane sulfonate (and related perfluorobutane sulfonate, perfluorohexane sulfonate, perfluorooctanoic acid and perfluoropentane sulfonate); and medical/veterinary compounds sulfamethoxazole, sulfanilamide, dapson, sulfathiazole, sulfamethazine and diclofenac. The spatial distribution of EOCs varies widely, with concentrations declining with depth, consistent with a strong dominant vertical flow control. Groundwater EOC concentrations in Patna were found to peak within ∼10 km distance from the River Ganges, indicating mainly urban inputs with some local pollution hotspots. A heterogeneous relationship between EOCs and population density likely reflects confounding factors including varying input types and controls (e.g. spatial, temporal), wastewater treatment infrastructure and groundwater abstraction. Strong seasonal agreement in EOC concentrations was observed. Co-existence of limited transformation products with associated parent compounds indicate active microbial degradation processes. This study characterizes key controls on the distribution of groundwater EOCs across the urban to rural transition near Patna, as a rapidly developing Indian city, and contributes to the wider understanding of the vulnerability of shallow groundwater to surface-derived contamination in similar environments.

Neonicotinoids (NEOs) have become the most widely used insecticides in the world. However, information on the NEO concentrations in tap water based on nationwide surveys is limited in China. In this study, the levels of six NEOs were measured in tap water samples collected from 38 cities in China. Across all sampling locations, the overall frequency of detection for at least one NEO was 100%, indicating that NEOs are ubiquitous in tap water from China. Imidacloprid was the most abundant NEO (median: 7.59 ng/L), followed by (in decreasing order) clothianidin (5.46 ng/L), dinotefuran (4.55 ng/L), thiamethoxam (4.50 ng/L), acetamiprid (2.72 ng/L), and thiacloprid (0.38 ng/L). Significantly positive correlations (r = 0.655–0.902, p < 0.001) among all pairs of NEOs were observed, which showed that the sources of NEOs in tap water were common or related. Across all sampling locations, regional differences in concentrations (ΣNEOs; sum of six NEOs varied from 0.79 ng/L to 415 ng/L) were observed within China, showing an increasing trend from northern to southern China. Although the estimated daily intake of NEOs were much lower than the reference dose, the potential health risk of NEOs via tap water consumption should raise more public concern considering the high detection rates of NEOs in tap water.

The on-going and extensive use of neonicotinoids occur in orchards. However, it is still unknown whether and how orchard management affects soil properties, especially the contents and structure of soil organic matter during orchard development, and their further influences on neonicotinoid persistence. Here, surface soil samples were collected from the citrus orchards with different cultivation ages (1, 10, 14, and 20 years), and their physicochemical properties were determined. Changes in the chemical structure of soil organic matter (SOM) were furtherly examined using solid-state CP/TOSS ¹³C NMR. Then, the sorption isotherms of imidacloprid in these soils were investigated. The sorption coefficient (Kd) of imidacloprid at Cₑ of 0.05 mg/L in the orchard soils increased by 19.4–23.3%, along a 20-year chronosequence of cultivation, which should be mainly ascribed to the increase of SOM. However, the organic carbon-normalized sorption coefficient (Kₒc, sorption per unit mass of OM) of imidacloprid declined with increasing cultivation ages. Moreover, the polar and aliphatic domains of SOM had a significantly positive relation to the Kₒc of imidacloprid, suggesting its key role in governing imidacloprid sorption. The results highlighted that reasonable management measures could be adopted to control the occurrence and fate of neonicotinoids in soils, mainly by affecting the content and quality of SOM.

The fate of many chemicals in the environment, particularly contaminants of emerging concern (CEC), have been characterised to a limited extent with a major focus on occurrence in water. This study presents the characterisation, distribution and fate of multiple chemicals including pharmaceuticals, recreational drugs and pesticides in surface water, sediment and fauna representing different food web endpoints in a typical UK estuary (River Colne, Essex, UK). A comparison of contaminant occurrence across different benthic macroinvertebrates was made at three sites and included two amphipods (Gammarus pulex &Crangon crangon), a polychaete worm (Hediste diversicolor) and a gastropod (Peringia ulvae). Overall, multiple contaminants were determined in all compartments and ranged from; <LOQ – 386 ng L⁻¹ in surface water (n = 59 compounds), <LOQ – 146 ng g⁻¹ in sediment (n = 39 compounds) and <LOQ – 91 ng g⁻¹ biota (n = 33 compounds). H. diversicolor and P. ulvae (sediment dwellers) showed greater chemical body burden compared with the two swimming amphipod species sampled (up to 2.5 - 4-fold). The most frequently determined compounds in biota (100%, n = 36 samples) included; cocaine, benzyoylecgonine, carbamazepine, sertraline and diuron. Whilst some of the highest concentrations found were in species H. diverscolor and P. ulvae for psychoactive pharmaceuticals including citalopram (91 ng g⁻¹), sertraline (69 ng g⁻¹), haloperidol (66 ng g⁻¹) and the neonicotinoid, imidacloprid (33 ng g⁻¹) Sediment was noted as an important exposure route for these benthic dwelling organisms and will be critical to monitor in future studies. Overall, the analysis of multiple species and compartments demonstrates the importance of including a range of exposure pathways in order to appropriately assess chemical fates and associated risks in the aquatic environment.