Norway spurce (Picea abies L.) is the dominating tree species in the Silesian Beskid Mountains. The mountain forests are under severe climatic conditions, such as low temperatures, short growing season, strong winds, high annual precipitation, a long-time snow cover. The forests have been exposed also for about half century to gaseous and dust pollutants emitted by large industrial centres in the Czech Republic, Germany and Poland. Anthropogenic impacts may influence negatively the growth of tree fine roots, can develop ectomycorrhizas and alter the communities of ectomycorrhizal fungi and other microorganisms in soil. Ectomycorrhizal communities at the both forest sites were dominated by the same two morphotypes, which were further studied using the PCP-RFLP analysis for identification of the fungal species

Ectomycorrhizal types were quantified in soil cores taken from 0-5 cm horizon. Nine ectomycorrhizal types were distinguished on roots of pines grown close to the copper smelter, 17 on trees grown in vicinity of aluminium smelter versus 25 found on trees grown near phosphate fertilizer plant and from control trees. Molecular studies were performed to give a greater resolution of how emission from different sources influenced mycorrhizal fungal community of Scots pine

A long-term effect of heavy metal pollution originating from Copper Smelter and Refinery Division Glogow on a Scots pine stand was carried out in the protective zone surrounding the smelting/refining plant. The effect of enhanced Cu, Pb and Zn availability on the structure of ectomycorrhizal communities was assessed above- and below-ground among several subplots dominated by Scots pine (Pinus sylvestris). Three different approaches were performed: sporocarp inventories, morphological analysis of ectomycorrhizal roots and molecular techniques based on PCR-RFLP method. Only a few sporocarps of ectomycorrhizal species were observed