The increasing production and applications of graphene oxide (GO, a novel carbon nanomaterial) have raised numerous environmental concerns regarding its ecological risks. Triphenyl phosphate (TPhP) disperses in water and poses an increasing hazard to the ecosystem and human health. It is critical to study the environmental responses and molecular mechanisms of GO and TPhP together to assess both chemicals; however, this information is lacking. The present work revealed that GO promoted the bioaccumulation of TPhP in zebrafish larvae by 5.0%–24.3%. The TPhP-induced growth inhibition of embryos (malformation, mortality, heartbeat, and spontaneous movement) at environmentally relevant concentrations was significantly amplified by GO, and these results were supported by the downregulated levels of genes and proteins associated with cytoskeletal construction and cartilage and eye development. TPhP induced negligible alterations in the genes or proteins involved in oxidative stress and apoptosis, but those related proteins were all upregulated by GO. GO and TPhP coexposure activated the mTOR signaling pathway and subsequently promoted apoptosis in zebrafish by potentiating the oxidative stress induced by TPhP, presenting synergistic toxicity. These findings highlight the potential risks and specific molecular mechanisms of combining emerging carbon nanomaterials with coexisting organic contaminants.

With the intended application of engineered nanomaterials (ENMs) in agriculture, accurate assessment the effect of these ENMs on soil microbial communities is especially necessary. Here, maize plants were cultivated in soil amended by SiO₂, TiO₂, and Fe₃O₄ ENMs (100 mg kg⁻¹ soil) for four weeks. The impact of ENMs on bacterial community structure of the rhizosphere soil was investigated by using high-throughput sequencing. In addition, metabolites of maize rhizosphere soil were quantified by gas chromatography-mass spectrometry (GC-MS) based metabolomics. We found that the disturbance of ENMs on soil microbes are in the follow of Fe₃O₄>TiO₂>SiO₂. Exposure of Fe₃O₄ ENMs significantly reduced the abundance of nitrogen-fixation related bacteria Bradyrhizobiaceae (from 2.94% to 2.40%) and iron-redox bacteria Sediminibacterium (from 2.15% to 2.07%). Additionally, Fe₃O₄ ENMs significantly increased populations of Nocardioides (from 1.63% to 1.77%), Chitinophaga sancti (from 1.12% to 2.08%), Pantoea (from 1.31% to 2.22%), Rhizobiumand (from 1.41% to 1.74%) and Burkholderia-Paraburkholderia (from 1.50% to 2.09%), which are associated with carbon cycling and plant growth promoting. This study provides a perspective on the response of rhizosphere microbial community and low molecular weight metabolites to ENMs exposure, providing a comprehensive understanding of the environmental risk of ENMs.

For decades, a vast array of innovative biosorbents have been found out and used in the removal of heavy metals, including bacteria, algae and fungi, etc. Although extensive biological species have been tried as a biosorbent for heavy metals removal, for removal efficiency or economy efficiency limited, it has failed to make a substantial breakthrough in practical application. Thus, many improved methods based on biosorbents emerged. In this review, based on the literature and our research results, we highlight three types of novel methods for biosorbents removal of heavy metals: chemical modification of biosorbents; biomass and chemical materials combination; multiple biomass complex systems. We mainly focus on their configuration, biosorption performance, their creation method, regeneration/reuse, their application and development in the future. Through the comparative analysis of various methods, we think that intracellular autogenous nanomaterials may open up another window in biosorption of heavy metals area. At the same time, the combination of various treatment methods will be the development tendency of heavy metal pollution treatment in the future.

Our previous study showed heritable reproductive toxicity in the nematode Caenorhabditis elegans after multigenerational exposure to AgNO₃ and silver nanoparticles (Ag-NPs). The aim of this study was to determine whether such inheritable effects are correlated with induced germline mutations in C. elegans. Individual C. elegans lineages were exposed for 10 generations to equitoxic concentrations at EC₃₀ of AgNO₃, Ag-NPs, and sulfidized Ag-NPs (sAg-NPs), a predominant environmentally transformed product of pristine Ag-NPs. The mutations were detected via whole genome DNA sequencing approach by comparing F₀ and F₁₀ generations. An increase in the total number of variants, though not statistically significant, was observed for all Ag treatments and the variants were mainly contributed by single nucleotide polymorphisms (SNPs). This potentially contributed towards reproductive as well as growth toxicity shown previously after ten generations of exposure in every Ag treatment. However, despite Ag-NPs and AgNO₃ inducing stronger reproductive toxicity than sAg-NPs, exposure to sAg-NPs resulted in higher mutation accumulation with significant increase in the number of transversions. Thus our results suggest that other mechanisms of inheritance, such as epigenetics, may be at play in Ag-NP- and AgNO₃-induced multigenerational and transgenerational reproductive toxicity.

Antibiotics as emerging pharmaceutical pollutants have seriously not only threatened human life and animal health security, but also caused environmental pollution. It has drawn enormous attention and research interests in the study of antibiotics removal from aqueous environments. Graphene, an interesting one-atom-thick, 2D single-layer carbon sheet with sp² hybridized carbon atoms, has become an important agent for removal of antibiotic, owing to its unique physiochemical properties. Recently, a variety of graphene-based nanomaterials (GNMs) are reported to efficiently remove antibiotics from aqueous solutions by different technologies. In this review, we summarize different structure and properties of GNMs for the removal of antibiotics by adsorption. Meanwhile, advanced oxidation processes (AOPs), such as photocatalysis, Fenton process, ozonation, sulfate radical and combined AOPs by the aid of GNMs are summarized. Finally, the opportunities and challenges on the future scope of GNMs for removal of antibiotics from aqueous environments are proposed.

Nanotechnology and use of nanomaterials (NMs) improve life quality, economic growth and environmental health. However, the increasing production and use of NMs in commercial products has led to concerns about their potential toxicity on human and environment health, as well as its toxicological classification and regulation. In this context, there is an urgent need to standardize and validate procedures for nanotoxicity testing. Since the zebrafish embryotoxicity test (ZET) has been indicated as a suitable approach for the toxicity assessment of traditional and emergent pollutants, the aim of this review is to summarize the existing literature on embryotoxic and teratogenic effects of NMs on zebrafish. In addition, morphological changes in zebrafish embryos induced by NMs were classified in four reaction models, allowing classification of the mode of action and toxicity of different types of NM. Revised data showed that the interaction and bioaccumulation of NMs on zebrafish embryos were associated to several toxic effects, while the detoxification process was limited. In general, NMs induced delayed hatching, circulatory changes, pigmentation and tegumentary alterations, musculoskeletal disorders and yolk sac alterations on zebrafish embryos. Recommendations for nanotoxicological tests are given, including guidance for future research. This review reinforces the use of the ZET as a suitable approach to assess the health risks of NM exposure.

Herein, we utilize sequential extraction and high-throughput sequencing to investigate the effects of nanomaterial additives on As volatilization from flooded soils. We reveal that maximum volatilization is achieved in the fourth week and is followed by stabilization. The extent of volatilization decreased in the order of control > nano-zerovalent iron >40-nm hydroxyapatite > nano-Fe₃O₄ > 20-nm hydroxyapatite > multilayer graphene oxide > high-quality graphene oxide. The most abundant forms of As in soil corresponded to As-Fe and Al oxides. In soil with low levels of As pollution, the contents of these species increased after treatment with graphene oxides but decreased after treatment with other nanomaterials, with an opposite trend observed for soil with high levels of As pollution. The addition of nanomaterials influenced the activity of soil enzymes, e.g., hydroxyapatites affected the activities of urease and alkaline phosphatase, whereas graphene oxides significantly impacted that of peroxidase (P < 0.05). The addition of nanomaterials (which can potentially inhibit microbial growth) affected As levels by influencing the amount of As volatilized from polluted soil. Moreover, As volatilization, enzyme activity, and As speciation were observed to be mutually correlated (e.g., volatilization was negatively correlated to peroxidase activity and the contents of amorphous crystalline hydrous oxides of As-Fe and Al).

A means for identifying and prioritising the treatment of uncertainty (UnISERA) in environmental risk assessments (ERAs) is tested, using three risk domains where ERA is an established requirement and one in which ERA practice is emerging. UnISERA's development draws on 19 expert elicitations across genetically modified higher plants, particulate matter, and agricultural pesticide release and is stress tested here for engineered nanomaterials (ENM). We are concerned with the severity of uncertainty; its nature; and its location across four accepted stages of ERAs. Using an established uncertainty scale, the risk characterisation stage of ERA harbours the highest severity level of uncertainty, associated with estimating, aggregating and evaluating expressions of risk. Combined epistemic and aleatory uncertainty is the dominant nature of uncertainty. The dominant location of uncertainty is associated with data in problem formulation, exposure assessment and effects assessment. Testing UnISERA produced agreements of 55%, 90%, and 80% for the severity level, nature and location dimensions of uncertainty between the combined case studies and the ENM stress test. UnISERA enables environmental risk analysts to prioritise risk assessment phases, groups of tasks, or individual ERA tasks and it can direct them towards established methods for uncertainty treatment.

Nanomaterials are increasingly being used to improve the properties and functions of common building materials. A new type of self-cleaning cement incorporating TiO2 nanomaterials (TiO2-NMs) with photocatalytic properties is now marketed. This promising cement might provide air pollution-reducing properties but its environmental impact must be validated. During cement use and aging, an altered surface layer is formed that exhibits increased porosity. The surface layer thickness alteration and porosity increase with the cement degradation rate. The hardened cement paste leaching behavior has been fully documented, but the fate of incorporated TiO2-NMs and their state during/after potential release is currently unknown. In this study, photocatalytic cement pastes with increasing initial porosity were leached at a lab-scale to produce a range of degradation rates concerning the altered layer porosity and thickness. No dissolved Ti was released during leaching, only particulate TiO2-NM release was detected. The extent of release from this batch test simulating accelerated worst-case scenario was limited and ranged from 18.7 ± 2.1 to 33.5 ± 5.1 mg of Ti/m2 of cement after 168 h of leaching. TiO2-NMs released into neutral aquatic media (simulate pH of surface water) were not associated or coated by cement minerals. The TiO2-NM release mechanism is suspected to start from freeing of TiO2-NMs in the altered layer pore network due to partial cement paste dissolution followed by diffusion into the bulk pore solution to the surface. The extent of TiO2-NM release was not solely related to the cement degradation rate.

Although nanoparticles are increasingly investigated, their impact on the availability of food (i.e., algae) at the bottom of food chains remains unclear. It is, however, assumed that algae, which form heteroagglomerates with nanoparticles, sediment quickly limiting the availability of food for primary consumers such as Daphnia magna. As a consequence, it may be hypothesized that this scenario – in case of fundamental importance for the nanoparticles impact on primary consumers – induces a similar pattern in the life history strategy of daphnids relative to situations of food depletion. To test this hypothesis, the present study compared the life-history strategy of D. magna experiencing different degrees of food limitation as a consequence of variable algal density with daphnids fed with heteroagglomerates composed of algae and titanium dioxide nanoparticles (nTiO2). In contrast to the hypothesis, daphnids’ body length, weight, and reproduction increased when fed with these heteroagglomerates, while the opposite pattern was observed under food limitation scenarios. Moreover, juvenile body mass, and partly length, was affected negatively irrespective of the scenarios. This suggests that daphnids experienced – besides a limitation in the food availability – additional stress when fed with heteroagglomerates composed of algae and nTiO2. Potential explanations include modifications in the nutritious quality of algae but also an early exposure of juveniles to nTiO2.