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Toxicity-associated changes in the invasive cyanobacterium Cylindrospermopsis raciborskii in response to nitrogen fluctuations
2018
Yang, Yiming | Chen, Youxin | Cai, Fangfang | Liu, Xiang | Wang, Yilang | Li, Renhui
The cyanobacterium Cylindrospermopsis raciborskii is of particular concern due to its ability to fix nitrogen (N), sporadic bloom, potential toxicity and apparent invasiveness. However, the toxicity associated behavior and response of toxic C. raciborskii under N fluctuations in water have been poorly investigated. The present study initiated based on the field survey in which Cylindrospermopsis species was found to have a high fitness under nitrate concentrations fluctuating from 0.02 mg L−1 to 2.90 mg L−1 in Chinese freshwater lakes. Examination on the role of short-term N fluctuations was conducted in two C. raciborskii strains which were exposed to a range of N concentrations supplied in two patterns, namely one-time pattern and ten-time pattern in which the equal amount of N was divided into ten-time accretions. The results showed the growth of both strains were not vulnerable to the transient nutrient fluctuations. The toxic strain showed considerable toxicological flexibility with the highest yield of cylindrospermopsin (CYN) obtained in the absence of N and the lowest in full medium. Generally, larger amounts of total CYN were observed at lower N levels, indicating that N deficiency promoted the intracellular accumulation and simultaneously restrained the extracellular release of CYN. Furthermore, CYN production was significantly different in two N supply patterns. The maximum quotas of intracellular and extracellular CYN in one-time pattern were respectively 2.79–3.53 and 3.94–7.20 times higher compared to the ten-time pattern. To our knowledge, our results are the first evidence of toxicity variations of C. raciborskii to the impermanent N fluctuations, shedding new light on its toxicological plasticity.
Show more [+] Less [-]Effects of mercury addition on microbial community composition and nitrate removal inside permeable reactive barriers
2018
Hiller-Bittrolff, Kenly | Foreman, Kenneth | Bulseco-McKim, Ashley N. | Benoit, Janina | Bowen, Jennifer L.
Permeable reactive barriers (PRBs) remove nitrogen from groundwater by enhancing microbial denitrification. The PRBs consist of woodchips that provide carbon for denitrifiers, but these woodchips also support other anaerobic microbes, including sulfate-reducing bacteria. Some of these anaerobes have the ability to methylate inorganic mercury present in groundwater. Methylmercury is hazardous to human health, so it is essential to understand whether PRBs promote mercury methylation. We examined microbial communities and geochemistry in fresh water and sulfate-enriched PRB flow-through columns by spiking replicates of both treatments with mercuric chloride. We hypothesized that mercury addition could alter bacterial community composition to favor higher abundances of genera containing known methylating taxa and that the sulfate-rich columns would produce more methylmercury after mercury addition, due mainly to an increase in abundance of sulfate reducing bacteria (SRB). However, methylmercury output at the end of the experiment was not different from output at the beginning, due in part to coupled Hg methylation and demethylation. There was a transient reduction in nitrate removal after mercury addition in the sulfate enriched columns, but nitrate removal returned to initial rates after two weeks, demonstrating resilience of the denitrifying community. Since methylmercury output did not increase and nitrate removal was not permanently affected, PRBs could be a low cost approach to combat eutrophication.
Show more [+] Less [-]Long-term interactive effects of N addition with P and K availability on N status of Sphagnum
2018
Chiwa, Masaaki | Sheppard, Lucy J. | Leith, Ian D. | Leeson, Sarah R. | Tang, Y Sim | Neil Cape, J.
Little information exists concerning the long-term interactive effect of nitrogen (N) addition with phosphorus (P) and potassium (K) on Sphagnum N status. This study was conducted as part of a long-term N manipulation on Whim bog in south Scotland to evaluate the long-term alleviation effects of phosphorus (P) and potassium (K) on N saturation of Sphagnum (S. capillifolium). On this ombrotrophic peatland, where ambient deposition was 8 kg N ha−1 yr−1, 56 kg N ha−1 yr−1 of either ammonium (NH4+, Nred) or nitrate (NO3−, Nox) with and without P and K, were added over 11 years. Nutrient concentrations of Sphagnum stem and capitulum, and pore water quality of the Sphagnum layer were assessed. The N-saturated Sphagnum caused by long-term (11 years) and high doses (56 kg N ha−1 yr−1) of reduced N was not completely ameliorated by P and K addition; N concentrations in Sphagnum capitula for Nred 56 PK were comparable with those for Nred 56, although N concentrations in Sphagnum stems for Nred 56 PK were lower than those for Nred 56. While dissolved inorganic nitrogen (DIN) concentrations in pore water for Nred 56 PK were not different from Nred 56, they were lower for Nox 56 PK than for Nox 56 whose stage of N saturation had not advanced compared to Nred 56. These results indicate that increasing P and K availability has only a limited amelioration effect on the N assimilation of Sphagnum at an advanced stage of N saturation. This study concluded that over the long-term P and K additions will not offset the N saturation of Sphagnum.
Show more [+] Less [-]Seasonal variabilities in chemical compounds and acidity of aerosol particles at urban site in the west Pacific
2018
Pan, Xiaole | Uno, Itsushi | Wang, Zhe | Yamamoto, Shigekazu | Hara, Yukari | Wang, Zifa
Mass concentrations of chemical compounds in both PM2.5 (particle aerodynamic diameter, Dp < 2.5 μm) and PM2.5-10 (2.5 < Dp < 10 μm), and acidity of aerosol particles were measured at an urban site in western Japan using a continuous dichotomous Aerosol Chemical Speciation Analyzer (ACSA-12) throughout 2014. Mass concentrations of both PM2.5 and sulfate had distinct seasonal variabilities with maxima in spring and winter, mostly due to long-range transport with the prevailing westerly wind. Mass concentration of nitrate in PM2.5 (fNO3) showed an obvious warm-season-low and cold-season-high pattern as a result of both gas-aerosol phase equilibrium processes under high temperature conditions as well as transport. Nitrate in PM2.5-10 (cNO3) increased during long-range transport of dust, implying the great importance of heterogeneous processes at the surface of coarse mode particles. In this study, Δ[H+] (derived from the difference in pH of extract liquid with/without sampling) was used to indicate the acidity of particles. We found that acidity of particles in PM2.5 (fΔH) was mostly positive with a maximum in August because of the large fraction of nitrate and sulfate. Acidity of particles in PM2.5-10 (cΔH) was negative in winter and spring due to presence of alkaline matter from crustal sources. This study highlights the great importance of anthropogenic pollutants on the acidity of particles in the western Pacific Ocean and further impact on the marine environment and climate.
Show more [+] Less [-]Nitrogen source track and associated isotopic dynamic characteristic in a complex ecosystem: A case study of a subtropical watershed, China
2018
Hao, Zhuo | Zhang, Xinyu | Gao, Yang | Xu, Zhiwei | Yang, Fengting | Wen, Xuefa | Wang, Yueming
By identifying the main sources of nitrate (NO3−) can obtain useful information to support the management of NO3− pollution, particularly in subtropical catchments with shallow drinking water wells. This study used water chemistry and dual stable isotopes δ15N and δ18O methods to assess seasonal and spatial variations of NO3− in precipitation, surface water, and groundwater in an agricultural and forest subtropical catchment in Jiangxi Province, China. The maximum concentrations of nitrate-nitrogen (NO3−-N) and ammonium-nitrogen (NH4+-N) were 10.4 and 10.8 mg L−1in samples collected from 221 rainfall events from 2011 to 2013. About 4.4% and 12.3% NH4+-N concentrations of surface water and groundwater exceeded the thresholds of 1.0 and 0.2 mg L−1. The NO3−-N concentrations in surface water were closely correlated with NH4+-N concentrations in surface water and groundwater (r = −0.71 and r = −0.71, P < 0.05). The concentrations of NH4+-N and NO3−-N were significantly higher in a fishery pond and nearby drinking wells than in other monitoring points. Annual exports of NO3−-N and NH4+-N were 4.06 × 104 and 8.14 × 103 kg yr−1, respectively and NO3−-N is the main form of N loss. The δ15N values ranged from 0‰ to 20‰ in surface water and groundwater, and the δ18O values ranged from 0‰ to 15‰ and 1‰–13‰, respectively. Dual stable isotope natural abundance distribution and water chemistry [NO3−]/[Cl−] molar ratio information suggested that manure and sewage and soil N were the main sources of NO3− in surface water and manure and sewage in groundwater in summer and winter. In spring, water occurred denitrification and ammonium fertilizer, manure and sewage were the main sources of NO3− in surface water and groundwater which sampling points were closer residential area and fish ponds than paddy field and local farmers used more Manure. Manure applications should be reasonable around drinking water wells to protect the drinking water quality.
Show more [+] Less [-]Joining empirical and modelling approaches to estimate dry deposition of nitrogen in Mediterranean forests
2018
García-Gómez, Héctor | Izquieta-Rojano, Sheila | Aguillaume, Laura | González-Fernández, Ignacio | Valiño, Fernando | Elustondo, David | Santamaría, Jesús M. | Àvila, Anna | Bytnerowicz, Andrzej | Bermejo, Victoria | Alonso, Rocío
In Mediterranean areas, dry deposition is a major component of the total atmospheric N input to natural habitats, particularly to forest ecosystems. An innovative approach, combining the empirical inferential method (EIM) for surface deposition of NO₃⁻ and NH₄⁺ with stomatal uptake of NH₃, HNO₃ and NO₂ derived from the DO₃SE (Deposition of Ozone and Stomatal Exchange) model, was used to estimate total dry deposition of inorganic N air pollutants in four holm oak forests under Mediterranean conditions in Spain. The estimated total deposition varied among the sites and matched the geographical patterns previously found in model estimates: higher deposition was determined at the northern site (28.9 kg N ha⁻¹ year⁻¹) and at the northeastern sites (17.8 and 12.5 kg N ha⁻¹ year⁻¹) than at the central-Spain site (9.4 kg N ha⁻¹ year⁻¹). On average, the estimated dry deposition of atmospheric N represented 77% ± 2% of the total deposition of N, of which surface deposition of gaseous and particulate atmospheric N averaged 10.0 ± 2.9 kg N ha⁻¹ year⁻¹ for the four sites (58% of the total deposition), and stomatal deposition of N gases averaged 3.3 ± 0.8 kg N ha⁻¹ year⁻¹ (19% of the total deposition). Deposition of atmospheric inorganic N was dominated by the surface deposition of oxidized N in all the forests (means of 54% and 42% of the dry and total deposition, respectively). The relative contribution of NO₂ to dry deposition averaged from 19% in the peri-urban forests to 11% in the most natural site. During the monitoring period, the empirical critical loads provisionally proposed for ecosystem protection (10–20 kg N ha⁻¹ year⁻¹) was exceeded in three of the four studied forests.
Show more [+] Less [-]Nitrate loads in sub-tropical headwater streams driven by intensive horticulture
2018
White, Shane A. | Santos, Isaac R. | Hessey, Samantha
Nitrogen runoff from fertiliser intensive land uses has become an issue worldwide, contributing to algal blooms, hypoxic waters and aquatic biodiversity losses. This study assessed potential nutrient pollution from blueberry farms in subtropical Australia and examines whether nutrient loads were driven by groundwater discharge and/or surface water runoff. Streams downstream of eight blueberry farms were compared to eight nearby control sites without any blueberry activity. In the 90 day sample period, there were three rain events >90 mm day⁻¹ that produced runoff sufficient to create flooding. Overall, the results revealed a clear link between blueberry farming and nitrogen runoff in headwater streams. While NOX (nitrate + nitrite) was the dominant nitrogen species downstream of blueberry farms, dissolved organic nitrogen (DON) was the dominant species in control sites. The concentrations and loads of NOₓ were one order of magnitude lower in the eight non-blueberry (6.3 ± 2.0 μmol L⁻¹; 1.6 ± 1.2 kg N-NOX ha⁻¹ yr⁻¹) than the eight blueberry (56.9 ± 14.2 μmol L⁻¹; 21.8 ± 8.0 kg N-NOX ha⁻¹ yr⁻¹) sites. NOX concentrations and loads were highest following rain events. Radon (²²²Rn, a natural groundwater tracer) observations and low nitrogen concentration in groundwater samples further suggest that surface runoff dominates the delivery of nitrogen to the creeks investigated. NOX concentrations and loads in creeks correlated with blueberry farm density. At >15% of blueberry land use in a catchment, there was a detectable influence in NOX concentrations and loads in the headwater streams. Assuming that our load estimates can be up-scaled to annual nitrogen creek exports, and that local farmers use the recommended amount of fertiliser (121 kg N ha⁻¹ yr⁻¹), between 18 and 25% of the used fertiliser was lost to the creeks. This implies that there are opportunities for decreasing the use of fertilisers in this catchment and managing any nitrogen that escapes to the creeks.
Show more [+] Less [-]NOx promotion of SO2 conversion to sulfate: An important mechanism for the occurrence of heavy haze during winter in Beijing
2018
Ma, Jinzhu | Chu, Biwu | Liu, Jun | Liu, Yongchun | Zhang, Hongxing | He, Hong
In this study, concentrations of NOₓ, SO₂, O₃ and fine particles (PM₂.₅) were measured at three monitoring stations in Beijing during 2015. For extreme haze episodes during 25 Nov. - 3 Dec. 2015, observation data confirmed that high concentrations of NOₓ promoted the conversion of SO₂ to sulfate. Annual data confirmed that this is an important mechanism for the occurrence of heavy haze during winter in Beijing. Furthermore, in situ perturbation experiments in a potential aerosol mass (PAM) reactor were carried out at Shengtaizhongxin (STZX) station during both clean and polluted days. The concentrations of SO₄²⁻, NH₄⁺, NO₃⁻ and organic aerosol were positively related to the concentration of added NO₂. These results provide definitive evidence that NO₂ can promote the conversion of SO₂ to sulfate. At the same time, we found that NO₂ can promote the formation of NH₄⁺ and organic compounds in the aerosols. Our results illustrate that strengthened controls of nitrogen oxides is a key step in reducing the fine particles level in China.
Show more [+] Less [-]Chemical composition and source apportionment of PM10 at an urban background site in a high–altitude Latin American megacity (Bogota, Colombia)
2018
Ramírez R., Omar | Sánchez de la Campa, A.M. | Amato, F. (Fulvio) | Catacolí, Ruth A. | Rojas, Néstor Y. | Rosa, Jesús de la
Bogota registers frequent episodes of poor air quality from high PM₁₀ concentrations. It is one of the main Latin American megacities, located at 2600 m in the tropical Andes, but there is insufficient data on PM₁₀ source contribution. A characterization of the chemical composition and the source apportionment of PM₁₀ at an urban background site in Bogota was carried out in this study. Daily samples were collected from June 2015 to May 2016 (a total of 311 samples). Organic carbon (OC), elemental carbon (EC), water soluble compounds (SO₄²⁻, Cl⁻, NO₃⁻, NH₄⁺), major elements (Al, Fe, Mg, Ca, Na, K, P) and trace metals (V, Cd, Pb, Sr, Ba, among others) were analyzed. The results were interpreted in terms of their variability during the rainy season (RS) and the dry season (DS). The data obtained revealed that the carbonaceous fraction (∼51%) and mineral dust (23%) were the main PM₁₀ components, followed by others (15%), Secondary Inorganic Compounds (SIC) (11%) and sea salt (0.4%). The average concentrations of soil, SIC and OC were higher during RS than DS. However, peak values were observed during the DS due to photochemical activity and forest fires. Although trace metals represented <1% of PM₁₀, high concentrations of toxic elements such as Pb and Sb on RS, and Cu on DS, were obtained. By using a PMF model, six factors were identified (∼96% PM₁₀) including fugitive dust, road dust, metal processing, secondary PM, vehicles exhaust and industrial emissions. Traffic (exhaust emissions + road dust) was the major PM₁₀ source, accounting for ∼50% of the PM₁₀. The results provided novel data about PM₁₀ chemical composition, its sources and its seasonal variability during the year, which can help the local government to define control strategies for the main emission sources during the most critical periods.
Show more [+] Less [-]The effects of organophosphorus insecticides and heavy metals on DNA damage and programmed cell death in two plant models
2018
Cortés-Eslava, Josefina | Gómez-Arroyo, Sandra | Risueño, Maria C. | Testillano, Pilar S.
The ubiquity of pollutants, such as agrochemicals and heavy metals, constitute a serious risk to human health. To evaluate the induction of DNA damage and programmed cell death (PCD), root cells of Allium cepa and Vicia faba were treated with two organophosphate insecticides (OI), fenthion and malathion, and with two heavy metal (HM) salts, nickel nitrate and potassium dichromate. An alkaline variant of the comet assay was performed to identify DNA breaks; the results showed comets in a dose-dependent manner, while higher concentrations induced clouds following exposure to OIs and HMs. Similarly, treatments with higher concentrations of OIs and HMs were analyzed by immunocytochemistry, and several structural characteristics of PCD were observed, including chromatin condensation, cytoplasmic vacuolization, nuclear shrinkage, condensation of the protoplast away from the cell wall, and nuclei fragmentation with apoptotic-like corpse formation. Abiotic stress also caused other features associated with PCD, such as an increase of active caspase-3-like protein, changes in the location of cytochrome C (Cyt C) toward the cytoplasm, and decreases in extracellular signal-regulated protein kinase (ERK) expression. Genotoxicity results setting out an oxidative via of DNA damage and evidence the role of the high affinity of HM and OI by DNA molecule as underlying cause of genotoxic effect. The PCD features observed in root cells of A. cepa and V. faba suggest that PCD takes place through a process that involves ERK inactivation, culminating in Cyt C release and caspase-3-like activation. The sensitivity of both plant models to abiotic stress was clearly demonstrated, validating their role as good biosensors of DNA breakage and PCD induced by environmental stressors.
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