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Identification and quantification of a novel nitrate-reducing community in sediments of Suquía River basin along a nitrate gradient Full text
2010
Reyna, Luciana | Wunderlin, Daniel Alberto | Genti-Raimondi, Susana
We evaluated the molecular diversity of narG gene from Suquía River sediments to assess the impact of the nitrate concentration and water quality on the composition and structure of the nitrate-reducing bacterial community. To this aim, a library of one of the six monitoring stations corresponding to the highest nitrate concentration was constructed and 118 narG clones were screened. Nucleotide sequences were associated to narG gene from alpha-, beta-, delta-, gammaproteobacteria and Thermus thermophilus. Remarkably, 18% of clones contained narG genes with less than 69% similarity to narG sequences available in databases. Thus, indicating the presence of nitrate-reducing bacteria with novel narG genes, which were quantified by real-time PCR. Results show a variable number of narG copies, ranging from less than 1.0 × 102 to 5.0 × 104 copies per ng of DNA, which were associated with a decreased water quality index monitored along the basin at different times.
Show more [+] Less [-]Sensitivity of agricultural runoff loads to rising levels of CO2 and climate change in the San Joaquin Valley watershed of California Full text
2010
Ficklin, Darren L. | Luo, Yuzhou | Luedeling, Eike | Gatzke, Sarah E. | Zhang, Minghua
The Soil and Water Assessment Tool (SWAT) was used to assess the impact of climate change on sediment, nitrate, phosphorus and pesticide (diazinon and chlorpyrifos) runoff in the San Joaquin watershed in California. This study used modeling techniques that include variations of CO2, temperature, and precipitation to quantify these responses. Precipitation had a greater impact on agricultural runoff compared to changes in either CO2 concentration or temperature. Increase of precipitation by ±10% and ±20% generally changed agricultural runoff proportionally. Solely increasing CO2 concentration resulted in an increase in nitrate, phosphorus, and chlorpyrifos yield by 4.2, 7.8, and 6.4%, respectively, and a decrease in sediment and diazinon yield by 6.3 and 5.3%, respectively, in comparison to the present-day reference scenario. Only increasing temperature reduced yields of all agricultural runoff components. The results suggest that agricultural runoff in the San Joaquin watershed is sensitive to precipitation, temperature, and CO2 concentration changes. Agricultural runoff is significantly affected by changes in precipitation, temperature, and atmospheric CO2 concentration.
Show more [+] Less [-]Chemical fluxes in time through forest ecosystems in the UK - Soil response to pollution recovery Full text
2010
Vanguelova, E.I. | Benham, S. | Pitman, R. | Moffat, A.J. | Broadmeadow, M. | Nisbet, T. | Durrant, D. | Barsoum, N. | Wilkinson, M. | Bochereau, F. | Hutchings, T. | Broadmeadow, S. | Crow, P. | Taylor, P. | Houston, T Durrant
Long term trend analysis of bulk precipitation, throughfall and soil solution elemental fluxes from 12 years monitoring at 10 ICP Level II forest sites in the UK reveal coherent national chemical trends indicating recovery from sulphur deposition and acidification. Soil solution pH increased and sulphate and aluminium decreased at most sites. Trends in nitrogen were variable and dependant on its form. Dissolved organic nitrogen increased in bulk precipitation, throughfall and soil solution at most sites. Nitrate in soil solution declined at sites receiving high nitrogen deposition. Increase in soil dissolved organic carbon was detected - a response to pollution recovery, changes in soil temperature and/or increased microbial activity. An increase of sodium and chloride was evident - a possible result of more frequent storm events at exposed sites. The intensive and integrated nature of monitoring enables the relationships between climate/pollutant exposure and chemical/biological response in forestry to be explored.
Show more [+] Less [-]Validation of regression models for nitrate concentrations in the upper groundwater in sandy soils Full text
2010
Sonneveld, M.P.W. | Brus, D.J. | Roelsma, J.
For Dutch sandy regions, linear regression models have been developed that predict nitrate concentrations in the upper groundwater on the basis of residual nitrate contents in the soil in autumn. The objective of our study was to validate these regression models for one particular sandy region dominated by dairy farming. No data from this area were used for calibrating the regression models. The model was validated by additional probability sampling. This sample was used to estimate errors in 1) the predicted areal fractions where the EU standard of 50 mg l−1 is exceeded for farms with low N surpluses (ALT) and farms with higher N surpluses (REF); 2) predicted cumulative frequency distributions of nitrate concentration for both groups of farms. Both the errors in the predicted areal fractions as well as the errors in the predicted cumulative frequency distributions indicate that the regression models are invalid for the sandy soils of this study area. This study indicates that linear regression models that predict nitrate concentrations in the upper groundwater using residual soil N contents should be applied with care.
Show more [+] Less [-]Ammonium and nitrate tolerance in lichens Full text
2010
Since lichens lack roots and take up water, solutes and gases over the entire thallus surface, these organisms respond more sensitively to changes in atmospheric purity than vascular plants. After centuries where effects of sulphur dioxide and acidity were in the focus of research on atmospheric chemistry and lichens, recently the globally increased levels of ammonia and nitrate increasingly affect lichen vegetation and gave rise to intense research on the tolerance of lichens to nitrogen pollution. The present paper discusses the main findings on the uptake of ammonia and nitrate in the lichen symbiosis and to the tolerance of lichens to eutrophication. Ammonia and nitrate are both efficiently taken up under ambient conditions. The tolerance to high nitrogen levels depends, among others, on the capability of the photobiont to provide sufficient amounts of carbon skeletons for ammonia assimilation. Lowly productive lichens are apparently predisposed to be sensitive to excess nitrogen.
Show more [+] Less [-]Adaptation to osmotic stress provides protection against ammonium nitrate in Pelophylax perezi embryos Full text
2010
Adaptation to osmotic stress provides protection against ammonium nitrate in Pelophylax perezi embryos Full text
2010
The negative effects of pollution on amphibians are especially high when animals are additionally stressed by other environmental factors such as water salinity. However, the stress provoked by salinity may vary among populations because of adaptation processes. We tested the combined effect of a common fertilizer, ammonium nitrate (0–90.3 mg N–NO3NH4/L), and water salinity (0–2‰) on embryos of two Pelophylax perezi populations from ponds with different salinity concentrations. Embryos exposed to the fertilizer were up to 17% smaller than controls. Survival rates of embryos exposed to a single stressor were always below 10%. The exposure to both stressors concurrently increased mortality rate (>95%) of embryos from freshwater. Since the fertilizer was lethal only when individuals were stressed by the salinity, it did not cause lethal effects on embryos naturally adapted to saline environments. Our results underscore the importance of testing multiple stressors when analyzing amphibian sensitivity to environmental pollution. Natural resistance to salinity minimizes the impact of chemical fertilizers on amphibian embryos.
Show more [+] Less [-]Adaptation to osmotic stress provides protection against ammonium nitrate in Pelophylax perezi embryos Full text
2010
Ortiz-Santaliestra, Manuel E. | Fernández, M. José | Lizana, Miguel | Marco, Adolfo | Ministerio de Ciencia e Innovación (España) | Junta de Castilla y León
The negative effects of pollution on amphibians are especially high when animals are additionally stressed by other environmental factors such as water salinity. However, the stress provoked by salinity may vary among populations because of adaptation processes. We tested the combined effect of a common fertilizer, ammonium nitrate (0–90.3 mg N–NO3NH4/L), and water salinity (0–2‰) on embryos of two Pelophylax perezi populations from ponds with different salinity concentrations. Embryos exposed to the fertilizer were up to 17% smaller than controls. Survival rates of embryos exposed to a single stressor were always below 10%. The exposure to both stressors concurrently increased mortality rate (>95%) of embryos from freshwater. Since the fertilizer was lethal only when individuals were stressed by the salinity, it did not cause lethal effects on embryos naturally adapted to saline environments. Our results underscore the importance of testing multiple stressors when analyzing amphibian sensitivity to environmental pollution. | Funding was provided by the Regional Government of Castilla y León (SA071A06) and the Ministry of Science and Innovation of Spain (CGL2005-0372). | Peer reviewed
Show more [+] Less [-]Treatment of Hydroponics Wastewater Using Constructed Wetlands in Winter Conditions Full text
2010
Gagnon, Vincent | Maltais-Landry, Gabriel | Puigagut Juárez, Jaume | Chazarenc, Florent | Brisson, Jacques
Hydroponics culture generates large amounts of wastewater that are highly concentrated in nitrate and phosphorus but contains almost no organic carbon. Constructed wetlands (CWs) have been proposed to treat this type of effluent, but little is known about the performance of these systems in treating hydroponic wastewater. In addition, obtaining satisfactory winter performances from CWs operated in cold climates remains a challenge, as biological pathways are often slowed down or inhibited. The main objective of this study was to assess the effect of plant species (Typha sp., Phragmites australis, and Phalaris arundinacea) and the addition of organic carbon on nutrient removal in winter. The experimental setup consisted of 16 subsurface flow CW mesocosms (1 m², HRT of 3 days) fed with 30 L d¹ of synthetic hydroponics wastewater, with half of the mesocosms fed with an additional source of organic carbon (sucrose). Carbon addition had a significant impact on nitrate and phosphate removal, with removal means of 4.9 g m⁻² d⁻¹ of NO₃-N and 0.5 g m⁻² d⁻¹ of PO₄-P. Planted mesocosms were generally more efficient than unplanted controls. Furthermore, we found significant differences among plant treatments for NO₃-N (highest removal with P. arundinacea) and COD (highest removal with P. australis/Typha sp.). Overall, planted wetlands with added organic carbon represent the best combination to treat hydroponics wastewater during the winter.
Show more [+] Less [-]N-Application Methods and Precipitation Pattern Effects on Subsurface Drainage Nitrate Losses and Crop Yields Full text
2010
Bakhsh, Allah | Kanwar, Ramesh S. | Baker, J. L.
Diverting the infiltrating water away from the zone of N application can reduce nitrate-nitrogen (NO₃-N) leaching losses to groundwater from agricultural fields. This study was conducted from 2001 through 2005 to determine the effects of N-application methods using a localized compaction and doming (LCD) applicator and spoke injector on NO₃-N leaching losses to subsurface drainage water and corn (Zea mays L.)-soybean (Glycine max L.) yields. The field experiments were conducted at the Iowa State University's northeastern research center near Nashua, Iowa, on corn-soybean rotation plots under chisel plow system having subsurface drainage ‘tile' system installed in 1979. The soils at the site are glacial till derived soils. The N-application rates of 168 kg-N ha⁻¹ were applied to corn only for both the treatments each replicated three times in a randomized complete block design. For combined 5 years, the LCD N-applicator in comparison with spoke injector showed lower flow weighted NO₃-N concentrations in tile water (16.8 vs. 20.1 mg L⁻¹) from corn plots, greater tile flow (66 vs. 49 mm), almost equivalent NO₃-N leaching loss with tile water (11.5 vs. 11.3 kg-N ha⁻¹) and similar corn grain yields (11.17 vs. 11.37 Mg ha⁻¹), respectively, although treatments effects were found to be non-significant (p = 0.05) statistically. The analysis, however, revealed that amount and temporal distribution of the growing season precipitation also affected the tile flow, NO₃-N leaching loss to subsurface drain water, and corn-soybean yields. Moreover, the spatial variability effects from plot to plot in some cases, resulted in differences of tile flow and NO₃-N leaching losses in the range of three to four times despite being treated with the same management practices. These results indicate that the LCD N-applicator in comparison with spoke injector resulted in lower flow weighted NO₃-N concentrations in subsurface drain water of corn plots; however, strategies need to be developed to reduce the offsite transport of nitrate leaching losses during early spring period from March through June.
Show more [+] Less [-]The Impact of Irrigated Agriculture on Water Quality of Rivers Kongoni and Sirimon, Ewaso Ng'iro North Basin, Kenya Full text
2010
Mutisya, David K. | Tole, Mwakio
This study aimed at determining the water quality of River Sirimon and River Kongoni, Ewaso Ng'iro North Basin, Kenya. Water quality analysis of these two rivers was done for a period of 5 months between November 2005 and February 2006. Portable Palintest equipment was used for the chemical analysis. The study established that there were sulphates concentrations of 22 mgL⁻¹ in the Kongoni River water associated with the use of commercial fertilisers as compared to mean values of 7 mgL⁻¹ along Sirimon River; phosphate concentrations were 1.3 mgL⁻¹ in Kongoni and 0.15 mgL⁻¹ in Sirimon and salinity 3 mgL⁻¹ in Kongoni and 0.47 mgL⁻¹ in Sirimon. On average, mean nitrates concentrations of 1.7 mgL⁻¹ were recorded for Kongoni River, which were higher than those recorded for Sirimon River (0.033 mgL⁻¹). These concentration levels were however within the standard levels set by WHO for example 50 mgL⁻¹ for nitrates (WHO 2008). River Kongoni has two major irrigated horticultural farms across it which were likely polluting the river during the time of this study.
Show more [+] Less [-]Mercury and Methylmercury Dynamics in a Coastal Plain Watershed, New Jersey, USA Full text
2010
Barringer, Julia L. | Riskin, Melissa L. | Szabó, Zoltán | Reilly, Pamela A. | Rosman, Robert | Bonin, Jennifer L. | Fischer, Jeffrey M. | Heckathorn, Heather A.
The upper Great Egg Harbor River watershed in New Jersey's Coastal Plain is urbanized but extensive freshwater wetlands are present downstream. In 2006-2007, studies to assess levels of total mercury (THg) found concentrations in unfiltered streamwater to range as high as 187 ng/L in urbanized areas. THg concentrations were <20 ng/L in streamwater in forested/wetlands areas where both THg and dissolved organic carbon concentrations tended to increase while pH and concentrations of dissolved oxygen and nitrate decreased with flushing of soils after rain. Most of the river's flow comes from groundwater seepage; unfiltered groundwater samples contained up to 177 ng/L of THg in urban areas where there is a history of well water with THg that exceeds the drinking water standard (2,000 ng/L). THg concentrations were lower (<25 ng/L) in unfiltered groundwater from downstream wetland areas. In addition to higher THg concentrations (mostly particulate), concentrations of chloride were higher in streamwater and groundwater from urban areas than in those from downstream wetland areas. Methylmercury (MeHg) concentrations in unfiltered streamwater ranged from 0.17 ng/L at a forest/wetlands site to 2.94 ng/L at an urban site. The percentage of THg present as MeHg increased as the percentage of forest + wetlands increased, but also was high in some urban areas. MeHg was detected only in groundwater <1 m below the water/sediment interface. Atmospheric deposition is presumed to be the main source of Hg to the wetlands and also may be a source to groundwater, where wastewater inputs in urban areas are hypothesized to mobilize Hg deposited to soils.
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