Urban and regional ozone (O₃) pollution is a public health concern and causes damage to ecosystems. Due to the diverse emission sources of O₃ precursors and the complex interactions of air dispersion and chemistry, identifying the contributing sources of O₃ pollution requires integrated analysis to guide emission reduction plans. In this study, the meteorological characteristics leading to O₃ polluted days (in which the maximum daily 8–h average O₃ concentration is higher than the China Class II National O₃ Standard (160 μg/m³)) in Guangzhou (GZ, China) were analyzed based on data from 2019. The O₃ formation regimes and source apportionments under various prevailing wind directions were evaluated using a Response Surface Modeling (RSM) approach. The results showed that O₃ polluted days in 2019 could be classified into four types of synoptic patterns (i.e., cyclone, anticyclone, trough, and high pressure approaching to sea) and were strongly correlated with high ambient temperature, low relative humidity, low wind speed, variable prevailing wind directions. Additionally, the cyclone pattern strongly promoted O₃ formation due to its peripheral subsidence. The O₃ formation was nitrogen oxides (NOₓ)-limited under the northerly wind, while volatile organic compounds (VOC)-limited under other prevailing wind directions. Anthropogenic emissions contributed largely to the O₃ formation (54–78%) under the westerly, southwesterly, easterly, southeasterly, or southerly wind, but only moderately (35–47%) under the northerly or northeasterly wind. Furthermore, as for anthropogenic contributions, local emission contributions were the largest (39–60%) regardless of prevailing wind directions, especially the local NOₓ contributions (19–43%); the dominant upwind regional emissions contributed 12–46% (e.g., contributions from Dongguan were 12–20% under the southeasterly wind). The emission control strategies for O₃ polluted days should focus on local emission sources in conjunction with the emission reduction of upwind regional sources.

Vegetable soils with high nitrogen input are hotspots of nitrous oxide (N₂O) and nitric oxide (NO), and biochar amended to soil has been documented to effectively decrease N₂O and NO emissions. However, the aging effects of biochar on soil N₂O and NO production and the relevant mechanisms are not thoroughly understood. A¹⁵N tracing microcosm study was conducted to clarify the responses of N₂O and NO production pathways to the biochar aging process in vegetable soil. The results showed that autotrophic nitrification was the predominant source of N₂O production. Biochar aging increased the O-containing functional groups while lowering the aromaticity and pore size. Fresh biochar enhanced the AOB-amoA gene abundance and obviously stimulated N₂O production by 15.5% via autotrophic nitrification and denitrification. In contrast, field-aged biochar markedly weakened autotrophic nitrification and denitrification and thus decreased N₂O production by 17.0%, as evidenced by the change in AOB-amoA and nosZI gene abundances. However, the amendment with artificially lab-aged biochar had no effect on N₂O production. With the extension of aging time, biochar application reduced the soil NO production dominated by nitrification. Changes in the N₂O and NO fluxes were closely associated with soil NH₄⁺-N and NO₂⁻-N contents, indicating that autotrophic nitrification played a critical role in NO production. Overall, our study demonstrated that field-aged biochar suppressed N₂O production via autotrophic nitrification and denitrification by regulating associated functional genes, but not for lab-aged biochar or fresh biochar. These findings improved our insights regarding the implications of biochar aging on N₂O and NO mitigation in vegetable soils.

Aquaculture has significant impacts on freshwater lakes, but plankton communities, as key components of the microbial food web, are rarely considered when assessing the impacts of aquaculture. Revealing the dynamics of plankton communities, including bacterioplankton, phytoplankton and zooplankton, under anthropological disturbances is critical for predicting the freshwater ecosystem functioning in response to future environmental changes. In the present study, we examined the impacts of aquaculture on water quality, plankton diversity and the co-occurrence patterns within plankton metacommunities in a shallow freshwater lake. The study zones are influenced by the 20-year historical intensive aquaculture, but now they are undergoing either ecological aquaculture or ecological restoration. Our results showed that ecological aquaculture was more efficient in nitrogen removal than ecological restoration. Moreover, lower bacterioplankton diversity but higher phytoplankton and zooplankton diversity were found in the ecological aquaculture and ecological restoration zones compared to the control zone. The lower network connectivity of the plankton metacommunities in the ecological aquaculture and ecological restoration zones indicated the decreasing complexity of potential microbial food web, suggesting a possible lower resistance of the plankton metacommunities to future disturbance. Furthermore, plankton communities of different trophic levels were driven under distinct mechanisms. The bacterioplankton community was primarily affected by abiotic factors, whereas the phytoplankton and zooplankton communities were explained more by trophic interactions. These results revealed the impacts of aquaculture on the plankton communities and their potential interactions, thereby providing fundamental information for better understanding the impacts of aquaculture on freshwater ecosystem functioning.

Coastal waters are confluences receiving large amounts of point and non-point sources of pollution. An attempt was made to explore microbial community interactions in response to carbon, nitrogen and metal pollution. Additionally, experiments were designed to analyze the influence of these factors on horizontal gene transfer (HGT). Shift in bacterial diversity dynamics by arsenic stress and nutrient addition in coastal waters was explored by metagenomics of microcosm setups. Phylogenetic analysis revealed equal distribution of Gammaproteobacteria (29%) and Betaproteobacteria (28%) in control microcosm. This proportional diversity from control switched to unique distribution of Gammaproteobacteria (44.5%)> Flavobacteria (17.7%)> Bacteriodia (11.92%)> Betaproteobacteria (11.52%) in microcosm supplemented with carbon, nitrogen and metal (C + N + M). Among metal-stressed systems, alpha diversity analysis indicated highest diversity of genera in C + N + M followed by N + M > C+M> metal alone. Arsenic and ampicillin sensitive E. coli XL1 blue and environmental strains (Vibrio tubiashii W85 and E. coli W101) were tested for efficiency of uptake of plasmid (P) pUCminusMCS (arsBᴿampᴿ) under varying stress conditions. Transformation experiments revealed that combined effect of carbon, nitrogen and metal on horizontal gene transfer (HGT) was significantly higher (p < 0.01) than individual factors. The effect of carbon on HGT was proved to be superior to nitrogen under metal stressed conditions. Presence of arsenic in experimental setups (P + M, P + N + M and P + C + M) enhanced the HGT compared to non-metal counterparts supplemented with carbon or nitrogen. Arsenic resistant bacterial isolates (n = 200) were tested for the ability to utilize various carbon and nitrogen substrates and distinct positive correlation (p < 0.001) was found between arsenic resistance and utilization of urea and nitrate. However, evident positive correlation was not found between carbon sources and arsenic resistance. Our findings suggest that carbon and nitrogen pollution in aquatic habitats under arsenic stress determine the microbial community dynamics and critically influence uptake of genetic material from the surrounding environment.

Japan receives nitrogenous air pollutants via long-range transport from China. However, emissions of nitrogenous air pollutants in China have stabilized or decreased in recent years. This study examined both the long-term trends in atmospheric nitrogen (N) deposition from the 1990s to the 2010s and the response of stream water nitrate (NO₃⁻) leaching from forested areas in western Japan. A long-term (1992–2018) temporal analysis of atmospheric N deposition in Fukuoka (western Japan) was conducted. Atmospheric bulk N deposition was collected at forested sites in a suburban forest (Swₑₛₜ) and a rural forest (Rwₑₛₜ) in western Japan during 2009–2018. Stream water samples were also collected from four locations at sites Swₑₛₜ and Rwₑₛₜ during the same period. Results showed that atmospheric N deposition in Fukuoka started to decrease from the mid-2000s at an annual rate of −2.5% yr⁻¹. The decrease in atmospheric N deposition was attributable mainly to decreased atmospheric ammonium (NH₄⁺) deposition, which caused greater contribution of NO₃⁻ deposition to atmospheric N deposition. Concentrations of NO₃⁻ in the stream water samples from three of the four locations decreased significantly at an annual rate of −3.7 to −0.7% yr⁻¹. However, stream water NO₃⁻ concentrations increased in one watershed where understory vegetation has been deteriorating owing to the increased deer population. This might weaken the recovery of N leaching from forested areas.

It is widely recognized that green infrastructures in urban ecosystems provides important ecosystem services, including air purification. The potential absorption of nitrogen oxides (NOₓ) by urban trees has not been fully quantified, although it is important for air pollution mitigation and the well-being of urban residents. In this study, four common tree species (Sophora japonica L., Fraxinus chinensis Roxb., Populus tomentosa Carrière, Sabina chinensis (L.)) in Beijing, China, were studied. The dual stable isotopes (¹⁵N and ¹⁸O) and a Bayesian isotope mixing model were applied to estimate the sources contributions of potential nitrogen sources to the roadside trees based on leaf and soil sampling in urban regions. The following order of sources contributions was determined: soil > dry deposition > traffic-related NOₓ. The capacity of urban trees for NOₓ removal in the city was estimated using a remote sensing and GIS approach, and the removal capacity was found to range from 0.79 to 1.11 g m⁻² a⁻¹ across administrative regions, indicating that 1304 tons of NOₓ could be potentially removed by urban trees in 2019. Our finding qualified the potential NOₓ removal by urban trees in terms of atmospheric pollution mitigation, highlighting the role of green infrastructure in air purification, which should be taken into account by stakeholders to manage green infrastructure as the basis of a nature-based approach.

Atmospheric peroxyacetyl nitrate (PAN) and ozone (O₃) are two typical indicators for photochemical pollution that have adverse effects on the ecosystem and human health. Observation networks for these pollutants have been expanding in developed regions of China, such as North China Plain (NCP) and Pearl River Delta (PRD), but are sparse in Yangtze River Delta (YRD), meaning their concentration and influencing factors remain poorly understood. Here, we performed a one-year measurement of atmospheric PAN, O₃, particulate matter with aerodynamic diameter smaller than 2.5 μm (PM₂.₅), nitrogen oxides (NOₓ), carbon monoxide (CO), and meteorological parameters from December 2016 to November 2017 in Shanghai. Overall, high hourly maximum PAN and O₃ were found to be 7.0 and 185 ppbv in summer, 6.2 and 146 ppbv in autumn, 5.8 and 137 ppbv in spring, and 6.0 and 76.7 ppbv in winter, respectively. Continental air masses probably carried atmospheric pollutants to the sampling site, while frequent maritime winds brought in less polluted air masses. Furthermore, positive correlations (R: 0.72–0.85) between PAN and O₃ were found in summer, indicating a predominant role of photochemistry in their formation. Unlike in summer, weak or no correlations between PAN and O₃ were featured during the other seasons, especially in winter, due to their different loss pathways. Unexpectedly, positive correlations between PAN and PM₂.₅ were found in all seasons. During summer, moderate correlation could be attributed to the strong photochemistry acting as a common driver in the formation of secondary aerosols and PAN. During winter, high PM₂.₅ might promote PAN production through HONO production, hence resulting in a good positive correlation. Additionally, the loss of PAN by thermal decomposition (TPAN) only accounted for a small fraction (ca. 1%) of the total (PAN + TPAN) during a typical winter episode, while it significantly reached 14.4 ppbv (71.1% of the total) in summer.

Constructed wetlands (CWs) inoculated with exogenous microbes have great potential for removing pollutants in adverse environments. The rapid loss of functional bacteria and the high cost of repeated additions of inoculum, however, limit the practical application of this technology. In this study, C–F2 immobilized bacteria (i.e., immobilized salt-tolerant bacterium Alishewanella sp. F2 incorporated with a carbon source) were developed and utilized in CWs for solving the above problems. A 60-day experiment demonstrated that bioaugmented CWs (Bio-CWs) with the addition of C–F2 immobilized bacteria into the bottom gravel layer of CW microcosms (B-CF2 treatment) exhibited high nitrogen removal efficiency under a saline condition (electrical conductivity of 15 mS/cm). We measured mean nitrate nitrogen (NO₃⁻-N) and total nitrogen (TN) removal percentages of 97.8% and 88.1%, respectively, which were significantly (p < 0.05) higher than those in Bio-CWs with microbial inoculum (MI-F2 treatment, 63.5% and 78.2%) and unbioaugmented CWs (CK, 48.7% and 67.2%). The TN content of the entire plant was significantly (p < 0.05) increased in B-CF2 (636.06 mg/microcosm) compared with CK (372.06 mg/microcosm). The relative abundances of the genera Alishewanella (i.e., the exogenous bacterium, 5.5%), Clostridium-XlVa (8.8%) and Bacteroides (21.1%) in B-CF2 were significantly (p < 0.05) higher than in MI-F2 and CK, which improved the denitrification capacity of CWs. Overall, a high denitrification efficiency and durability were achieved in the newly developed Bio-CWs (i.e., B-CF2 treatment) with immobilized bacteria under saline conditions, which provides an alternative technology for the rapid removal of nitrogen from saline wastewater.

The industrial production of insects for waste management or as a protein source is becoming vital to our society. Large volumes of manure are produced by concentrated animal facilities around the globe that must be managed, utilized, and disposed of properly. Flies offer a partial solution with their abilities to reduce these wastes and heavy metal pollutants. Meat and crop proteins are being supplemented by insect proteins for many feeds across the globe, yet science-based studies behind the mass-rearing of insects are still in their infancy. In the current study, the percent change in the composition of nutrients, heavy metals, and fiber, in dairy, poultry, and swine manure degraded by either black soldier fly (BSF) or house fly (HF) larvae was explored. Pre-digested and post-digested manure samples were collected from four independent studies that differed in production scale (number of larvae and feeding regimen): 1) BSF small-scale (100 larvae fed incrementally), 2) HF small-scale (100 larvae fed incrementally), 3) BSF large-scale (10,000 larvae fed a single time), and 4) HF large-scale (4,000 larvae fed a single time). Results indicate that nitrogen is a key nutrient impacted by larval digestion of manure by both species, regardless of scale. However, scale significantly impacted reductions of other nutrients, as did the type of manure in which the insects were reared. Ultimately, this study demonstrated that manure type and rearing scale impact the ability of BSF and HF larvae to reduce nutrients and heavy metals in manure, and thus insect management procedures need to be congruent with production emphases of the insects for waste management or protein products. Failure to take scale into consideration could lead to inaccurate assumptions related to industrialized efforts on this topic.

Nitrogen in urban stormwater has been widely studied, and effective management of nitrogen pollution is critical for improving urban stormwater and receiving water quality. This requires an in-depth understanding of the transport process and source contribution to both dissolved and particulate nitrogen in stormwater from urban catchments. In this study, 123 stormwater runoff samples were collected from an urban catchment during different rainfall events. Dissolved and particulate nitrogen concentrations in roof runoff, road runoff, and sewer flow were analyzed. The concentration of dissolved nitrogen was higher in roof runoff than in road runoff and sewer flow. However, the concentration of particulate nitrogen was lower in roof runoff than in road runoff and sewer flow. Isotopic analysis and Bayesian mixing models showed that road runoff was the largest source contributor of both nitrate and particulate organic nitrogen (PON) in sewer flow discharged from the study catchment. In addition, road runoff contributed the majority of PON associated with coarse particles (>105 μm), whereas PON associated with fine particles (<105 μm) was primarily washed-off of sewer sediments. The results provided several suggestions for the management of nitrogen pollution in urban catchments. This study could help to fully understand the transport and sources of nitrogen pollution in urban stormwater and provide recommendations to the government for implementing appropriate stormwater management strategies to minimize stormwater pollution.