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Biochar mitigates arsenic-induced human health risks and phytotoxicity in quinoa under saline conditions by modulating ionic and oxidative stress responses
2021
Shabbir, Arslan | Saqib, Muhammad | Murtaza, Ghulam | Abbas, Ghulam | Imran, Muhammad | Rizwan, Muhammad | Naeem, Muhammad Asif | Ali, Shafaqat | Rashad Javeed, Hafiz Muhammad
Arsenic (As) is a toxic metalloid and its widespread contamination in agricultural soils along with soil salinization has become a serious concern for human health and food security. In the present study, the effect of cotton shell biochar (CSBC) in decreasing As-induced phytotoxicity and human health risks in quinoa (Chenopodium quinoa Willd.) grown on As-spiked saline and non-saline soils was evaluated. Quinoa plants were grown on As contaminated (0, 15 and 30 mg kg⁻¹) saline and non-saline soils amended with 0, 1 and 2% CSBC. Results showed that plant growth, grain yield, stomatal conductance and chlorophyll contents of quinoa showed more decline on As contaminated saline soil than non-saline soil. The application of 2% CSBC particularly enhanced plant growth, leaf relative water contents, stomatal conductance, pigment contents and limited the uptake of As and Na as compared to soil without CSBC. Salinity in combination with As trigged the production of H₂O₂ and caused lipid peroxidation of cell membranes. Biochar ameliorated the oxidative stress by increasing the activities of antioxidant enzymes (SOD, POD, CAT). Carcinogenic and non-carcinogenic human health risks were greatly decreased in the presence of biochar. Application of 2% CSBC showed promising results in reducing human health risks and As toxicity in quinoa grown on As contaminated non-saline and saline soils. Further research is needed to evaluate the role of biochar in minimizing As accumulation in other crops on normal as well as salt affected soils under field conditions.
Show more [+] Less [-]Interactions between microplastics/nanoplastics and vascular plants
2021
Yin, Lingshi | Wen, Xiaofeng | Huang, Danlian | Du, Chunyan | Deng, Rui | Zhou, Zhenyu | Tao, Jiaxi | Li, Ruijin | Zhou, Wei | Wang, Zeyu | Chen, Haojie
Microplastics and nanoplastics are distributed in the environments universally. The interrelationship between vascular plants and micro/nanoplastics began to attract attention in recent years. Based on the relevant literatures collected from various databases, this review focuses on two topics: 1) the effect of vascular plants on the fate of micro/nanoplastics; 2) the effects of micro/nanoplastics on vascular plants. The review of the available studies reveals that vascular plants can act as sinks for microplastics and nanoplastics as their surfaces can adsorb these plastics; moreover, nanoplastics can be internalized by plants. Plastics on the surfaces and in the interiors of vascular plants can cause various phytotoxicity effects, including impacts on growth, photosynthesis, and oxidative stress. Furthermore, the results and mechanisms of phytotoxicity effects caused by microplastics or nanoplastics can be very different. However, knowledge gaps still exist in the relationships between micro/nanoplastics and vascular plants based on the analysis of available studies; thus, potential subjects for future studies were proposed, including the fates, analysis methods, influencing factors, mechanisms of phytotoxicity, and further influences of microplastics and nanoplastics in the vascular plant ecosystems. This study presents a review of micro/nanoplastics–vascular plant research and reaches a basis for future research.
Show more [+] Less [-]6-benzylaminopurine exposure induced development toxicity and behaviour alteration in zebrafish (Danio rerio)
2021
Yang, Mengying | Qiu, Jinyu | Zhao, Xin | Feng, XiZeng
6-benzylaminopurine (6-BA) is one of the first synthetic hormones and has been widely used in fruit cultivation, gardening and agriculture. However, excessive use of 6-BA will cause potential harm to the environment and humans. Therefore, our research focused on assessing the impact of 6-BA on the development and neurobehavior of zebrafish. The results showed that 6-BA had little effect on the embryos from 2 hpf to 10 hpf. However, delayed development, decreased survival and hatchability were observed under 30 and 40 mg/L 6-BA from 24 hpf. 6-BA also reduced surface tension of embryonic chorions at 24 hpf. In addition, 6-BA caused abnormal morphology and promoted the accumulation of oxidative stress. Transcription of genes in connection with development and oxidative stress was also strikingly altered. Results of movement assay showed that zebrafish were less active and their behavior was significantly inhibited under the 20 and 30 mg/L 6-BA treatments. Locomotion-related genes th and mao were down-regulated by gradient, while the transcription of dbh was upregulated at a low concentration (2 mg/L) but decreased as the concentration increased. Moreover, 6-BA exposure caused increased arousal and decreased sleep. Sleep/wake related genes hcrt and hcrtr2 were upregulated, but decreased at 30 mg/L, while the mRNA level of aanat2 was reduced in a concentration-dependent manner. To sum up, our results showed that 6-BA induced developmental toxicity, promoted the accumulation of oxidative stress, and damaged locomotion and sleep/wake behavior.
Show more [+] Less [-]Responses of the reproduction, population growth and metabolome of the marine rotifer Brachionus plicatilis to tributyl phosphate (TnBP)
2021
Zhang, Xin | Tang, Xuexi | Yang, Yingying | Sun, Zijie | Ma, Wenqian | Tong, Xin | Wang, Chengmin | Zhang, Xinxin
The typical alkyl organophosphorus flame retardant tributyl phosphate (TnBP) can leak from common products into the marine environment, with potential negative effects on marine organisms. However, risk assessments for TnBP regarding zooplankton are lacking. In this study, a marine rotifer, Brachionus plicatilis, was used to analyze the effect of TnBP (0.1 μg/L, environmental concentration; 1 and 6 mg/L) on reproduction, population growth, oxidative stress, mitochondrial function and metabolomics. Mortality increased as the TnBP concentration rose; the 24-h LC₅₀ value was 12.45 mg/L. All tested TnBP concentrations inhibited B. plicatilis population growth, with reproductive toxicity at the higher levels. Microstructural imaging showed ovary injury, the direct cause of reproductive toxicity. Despite elevated glutathione reductase activities, levels of reactive oxygen species and malonyldialdehyde increased under TnBP stress, indicating oxidative imbalance. TnBP induced mitochondrial malformation and activity suppression; the ROS scavenger N-acetylcysteine alleviated this inhibition, suggesting an internal connection. Nontargeted metabolomics revealed 398 and 583 differentially expressed metabolites in the 0.1 μg/L and 6 mg/L treatments relative to control, respectively, which were enriched in the pathways such as biosynthesis of amino acids, purine metabolism, aminoacyl-tRNA biosynthesis. According to metabolic pathway analysis, oxidative stress from purine degradation, mitochondrial dysfunction, disturbed lipid metabolism and elevated protein synthesis were jointly responsible for reproduction and population growth changes. This study echoes the results previously found in rotifer on trade-off among different life processes in response to environmental stress. Our systematic study uncovers the TnBP toxic mode of action.
Show more [+] Less [-]Polyethylene glycol acute and sub-lethal toxicity in neotropical Physalaemus cuvieri tadpoles (Anura, Leptodactylidae)
2021
Nascimento, Ítalo Freitas | Guimarães, Abraão Tiago Batista | Ribeiro, Fabianne | Rodrigues, Aline Sueli de Lima | Estrela, Fernanda Neves | Luz, Thiarlen Marinho da | Malafaia, Guilherme
Although many polymers are known by their toxicity, we know nothing about the impact of polyethylene glycol (PEG) on anurofauna. Its presence in different products and disposal in aquatic environments turn assessments about its impact on amphibians an urgent matter. Accordingly, we tested the hypothesis that short-time exposure (72 h) of tadpoles belonging to the species Physalaemus cuvieri (Anura, Leptodactylidae) to PEG induces oxidative stress and neurotoxicity on them. We observed that polymer uptake in P. cuvieri occurred after exposure to 5 and 10 mg/L of PEG without inducing changes in their nitrite levels neither at the levels of substances reactive to thiobarbituric acid. However, hydrogen peroxide and reactive oxygen species production was higher in animals exposed to PEG, whose catalase and superoxide dismutase levels were not enough to counterbalance the production of these reactive species. Therefore, this finding suggests physiological changes altering REDOX homeostasis into oxidative stress. In addition, the increased activity of acetylcholinesterase and butyrylcholinesterase, and reduction in superficial neuromasts, confirmed PEG’s neurotoxic potential. To the best of our knowledge, this is the first report on PEG’s biological impact on a particular amphibian species. The study has broadened the understanding about ecotoxicological risks associated with water pollution by these polymers, as well as motivated further investigations on its impacts on amphibians’ health and on the dynamics of their natural populations.
Show more [+] Less [-]Effects of the antineoplastic drug cyclophosphamide on the biochemical responses of the mussel Mytilus galloprovincialis under different temperatures
2021
Queirós, Vanessa | Azeiteiro, Ulisses M. | Barata, Carlos | Santos, Juan Luis | Alonso, Esteban | Soares, Amadeu M.V.M. | Freitas, Rosa
Cyclophosphamide (CP) is an antineoplastic drug widely used in chemotherapy treatments with high consumption rates and that has been detected in the aquatic environment. After being released into the aquatic environment, CP may cause adverse effects on aquatic organisms since antineoplastics are well-known cytotoxic, genotoxic, mutagenic and teratogenic drugs. Moreover, predicted environmental changes, such as the temperature rising, may alter the impacts caused by CP on organisms. Thus, the present study aimed to assess the effects caused by CP chronic exposure in the mussel Mytilus galloprovincialis, under actual and predicted warming scenarios. Organisms were exposed for 28 days to different concentrations of CP (10, 100, 500 and 1000 ng/L) at control (17 ± 1.0 °C) and increased (21 ± 1.0 °C) temperatures. Biochemical responses related to metabolic capacity, energy reserves, oxidative stress and neurotoxicity were assessed. The results showed that the organisms were able to maintain their metabolic capacity under all exposure conditions. However, their antioxidant defense mechanisms were activated mostly at higher CP concentrations being able to prevent cellular damage, even under the warming scenario. Overall, the present findings suggest that temperature rise may not alter the impacts of CP towards M. galloprovincialis.
Show more [+] Less [-]Serum concentrations of organochlorine pesticides, biomarkers of oxidative stress, and risk of breast cancer
2021
Miao, Yu | Rong, Mao | Li, Min | He, Heng | Zhang, Li | Zhang, Shanshan | Liu, Chong | Zhu, Ying | Deng, Yan-Ling | Chen, Pan-Pan | Zeng, Jia-Yue | Zhong, Rong | Mei, Su-Rong | Miao, Xiao-Ping | Zeng, Qiang
Studies have documented that exposure to organochlorine pesticides (OCPs) is linked with breast cancer, but the underlying biological mechanisms are still unknown. This study included 313 women diagnosed with breast cancer and 313 controls in Wuhan, China, and measured 18 OCPs in serum and 3 oxidative stress biomarkers in urine. Multivariable adjusted regression models were used to evaluate the associations among OCPs, oxidative stress biomarkers, and breast cancer. The mediating effect of oxidative stress was assessed by mediation analysis. We observed that most OCPs were positively associated with risk of breast cancer (all FDR-P values < 0.05 or 0.10). Moreover, we found that p,p'-DDT, p,p'-DDD, dieldrin, heptachlor, and heptachlor epoxide were positively associated with 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA) and 8-iso-prostaglandin F₂α (8-isoPGF₂α), which in turn were positively associated with risk of breast cancer. Mediation analysis indicated that HNE-MA and 8-isoPGF₂ɑ mediated the positive associations between these OCPs and risk of breast cancer, with mediating proportion ranging from 6.23% to 19.9%. Our results suggest that lipid peroxidation may mediate the positive associations between OCP exposures and risk of breast cancer.
Show more [+] Less [-]Experimental warming alleviates the adverse effects from tropospheric ozone on two urban tree species
2021
Xu, Sheng | Wang, Yijing | Zhang, Weiwei | Li, Bo | Du, Zhong | He, Xingyuan | Chen, Wei | Zhang, Yue | Li, Yan | Li, Maihe | Schaub, Marcus
Atmospheric warming and increasing tropospheric ozone (O₃) concentrations often co-occur in many cities of the world including China, adversely affecting the health status of urban trees. However, little information is known about the combined and interactive effects from increased air temperature (IT) and elevated O₃ (EO) exposures on urban tree species. Here, Ginkgo biloba and Populus alba ‘Berolinensis’ seedlings were subjected to IT (+2 °C of ambient air temperature) and/or EO (+2-fold ambient air O₃ concentrations) for one growing season by using open-top chambers. IT alone had no significant effect on physiological metabolisms at the early growing stage, but significantly increased photosynthetic parameters, antioxidative enzyme activities (P < 0.05). EO alone decreased physiological parameters except for increased oxidative stress. Compared to EO exposure alone, plants grown under IT and EO combined showed higher antioxidative and photosynthetic activity. There was a significant interactive effect between IT and EO on net photosynthetic rate, stomatal conductance, water use efficiency, the maximum quantum efficiency of PSII photochemistry, the actual quantum efficiency of PSII, enzyme activities, aboveground biomass and root/shoot ratio (P < 0.05), respectively. These results suggested that during one growing season, IT mitigated the adverse effect of EO on the tested plants. In addition, we found that G. biloba was more sensitive than P. alba ‘Berolinensis’ to both IT and EO, suggesting that G. biloba may be a good indicator species for climate warming and air pollution, particularly under environmental conditions as they co-occur in urban areas.
Show more [+] Less [-]Terrestrial dissolved organic matter source affects disinfection by-product formation during water treatment and subsequent toxicity
2021
Franklin, Hannah M. | Doederer, Katrin | Neale, Peta A. | Hayton, Joshua B. | Fisher, Paul | Maxwell, Paul | Carroll, Anthony R. | Burford, Michele A. | Leusch, Frederic D.L.
Restoring woody vegetation to riparian zones helps to protect waterways from excessive sediment and nutrient inputs. However, the associated leaf litter can be a major source of dissolved organic matter (DOM) leached into surface waters. DOM can lead to the formation of disinfection by-products (DBPs) during drinking water treatment. This study investigated the DBPs formed during chlorination of DOM leached from leaf litter and assessed the potential toxicity of DBPs generated. We compared the leachate of two native Australian riparian trees, Casuarina cunninghamiana and Eucalyptus tereticornis, and a reservoir water source from a catchment dominated by Eucalyptus species. Leachates were diluted to dissolved organic carbon concentrations equivalent to the reservoir (~9 mg L⁻¹). E. tereticornis leachates produced more trihalomethanes (THMs), haloacetic acids (HAAs), and haloketones after chlorination, while C. cunninghamiana produced more chloral hydrate and haloacetonitriles. Leachate from both species produced less THMs and more HAAs per mole of carbon than reservoir water. This may be because reservoir water had more aromatic, humic characteristics while leaf leachates had relatively more protein-like components. Using in vitro bioassays to test the mixture effects of all chemicals, chlorinated E. tereticornis leachate induced oxidative stress in HepG2 liver cells and bacterial toxicity more frequently and at lower concentrations than C. cunninghamiana and reservoir water. Overall, this study has shown that the DOM leached from litter of these species has the potential to generate DBPs and each species has a unique DBP profile with differing bioassay responses. E. tereticornis may pose a relatively greater risk to drinking water than C. cunninghamiana as it showed greater toxicity in bioassays. This implies tree species should be considered when planning riparian zones to ensure the benefits of vegetation to waterways are not offset by unintended increased DBP production and associated toxicity following chlorination at downstream drinking water intakes.
Show more [+] Less [-]Comparison of the chronic toxicities of graphene and graphene oxide toward adult zebrafish by using biochemical and phenomic approaches
2021
Audira, Gilbert | Lee, Jiann-Shing | Siregar, Petrus | Malhotra, Nemi | Rolden, Marri Jmelou M. | Huang, Jong-Chin | Chen, Kelvin H.-C. | Hsu, Hua-Shu | Hsu, Yuchun | Ger, Tzong-Rong | Hsiao, Chung-Der
Graphene (GR) and graphene oxide (GO) are widely being used as promising candidates for biomedical applications, as well as for bio-sensing, drug delivery, and anticancer therapy. However, their undesirable side effects make it necessary to assess further the toxicity and safety of using these materials. The main objective of the current study was to investigate the toxicities of GR and GO in predicted environmental relevant concentrations in adult zebrafish (Danio rerio), particularly on their behaviors, and conducted biochemical assays to elucidate the possible mechanism that underlies their toxicities. Zebrafish was chronically (∼14 days) exposed to two different doses of GR (0.1 and 0.5 ppm) or GO (0.1 and 1 ppm). At 14 ± 1 days, a battery of behavioral tests was conducted, followed by enzyme-linked immunosorbent assays (ELISA) test on the following day to inspect the alterations in antioxidant activity, oxidative stress, and neurotransmitters in the treated zebrafish brain. An alteration in predator avoidance behavior was observed in all treated groups, while GR-treated fish exhibited abnormal exploratory behavior. Furthermore, altered locomotor activity was displayed by most of the treated groups, except for the high concentration of the GR group. From the ELISA results, we discovered a high concentration of GR exposure significantly decreased several neurotransmitters and cortisol levels. Meanwhile, elevated reactive oxygen species (ROS) were displayed by the group treated with low and high doses of GR and GO, respectively. These significant changes would possibly affect zebrafish behaviors and might suggest the potential toxicity from GR and GO exposures. To sum up, the present study presented new evidence for the effects of GR and GO in zebrafish behavioral dysregulation. We hope these assessments can contribute to our understanding of graphene and graphene oxide biosafety.
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