Many previous studies evidenced very high levels of air pollution by ozone between the North Italy (Lombardy) and the Southern Switzerland (Canton Ticino). These levels are mostly attributed to the influence of the large urbanized area of Milan and to the urban and industrial settlements in the Po Valley. Recent researches revealed the existence of spread foliar ozone-like symptoms in many native plant species, both in the Swiss and in Italian sector. Experiments carried out in open top chambers in Canton Ticino allowed us to attribute the foliar symptoms to the ambient air pollution levels

There is still limited amount of information about the long-term and interactive effects of increased CO2 and O3 levels on larger forest trees growing under natural or semi-natural conditions. Elevated CO2 and O3 might affect the quality and quantity of leaf litter produced and thus change litter decomposition rates and nutrient cycling in the forest ecosystems severely. In this long-term field experiment we studied the effects of realistically increased CO2 and O3 levels on fine root and mycorrhiza growth in ozone-tolerant and ozone-sensitive silver birch clones by root ingrowth core method. We measured rhizosphere soil CO2 efflux plus assessed the total fungal biomass of fine roots and soil by ergosterol analysis

Toxic effects of photochemical smog on ponderosa and Jeffrey pines in the San Bernardino Mountains were discovered in the 1950s. It was revealed that ozone is the main cause of foliar injury manifested as chlorotic mottle and premature needle senescence. Various morphological, physiological and biochemical alterations in the affected plants have been reported over a period of about 40 years of multidisciplinary research. Recently, the focus of research has shifted from studying the effects of ozone to multiple pollutant effects. Recent studies have indicated that the combination of ozone and nitrogen may alter biomass allocation in pines towards that of deciduous trees, accelerate litter accumulation and increase carbon sequestration rates in heavily polluted forests

Emissions, especially of SO2 and to a certain degree of NOx have been reduced markedly in Slovakia during the 90s. Problems related to climate change and especially ozone are increasingly growing. The aim was to evaluate the long-term air pollution effect on forest ecosystems in mountain area with prevailing distribution of spruce through evaluation of spruce needle surface structure in relation to mineral nutrient status of trees. According to strong relationship between the coefficient of epicuticular wax degradation (Q) and accumulation of S and other elements it can be suggested that evaluation of needle surface structure by means of Q well reflects the surface status of needles

Increased ozone sensitivity of larger soil-growing trees with growth in the multi-year exposure was a result of several interactive senescence-related physiological factors: lower net photosynthesis to stomatal conductance ratio at the end of the growing season promoted high ozone uptake and low photosynthetic carbon gain, leading to onset of visible injuries and impaired bud formation. This was expected to affect negatively the early growth of the next year foliage, This clone showed a major change in allocation pattern during the early ontogeny at the expense of foliage growth towards the stem height increase

Wet-deposited - sulphur and nitrogen pollutants and ambient ozone are important anthropogenic factors affecting forest health. Regular assessment of chemistry of throughfall and precipitation water based on two-week sampling started in 1997. Passive samplers for detection of ozone concentration have been exposed on a network of monitoring stations during vegetation periods since 1998. In addition, in selected locations, UV absorption monitors for continuous O3 measurements were installed in 1999

The assessment of O3-like foliar symptoms is increasingly considered in monitoring programs aimed at evaluating air pollution effects on forests. The results showed considerable spatial and temporal variation of O3-like foliar symptoms: year, subplot adn the interaction between year and plot were found significant factors. Symptoms resulted lower in 2001; there were differences of foliar symptoms between different subplot and overall the variation between years are considerably different according to the plot being considered. These findings suggest that O3-like foliar symptoms recorded on a small plot selected without a formal statistical approach and with no consideration of the apparently high variability of symptoms expression risk to introduce a serious bias in the evaluation of the potential ozone impact at a given site and its trend

Two holm oak (Quercus ilex L.) sites in Tuscany, Central Italy, were studied in July 2000. Leaf morphological characteristics (area, dry weight, specific dry weight, water content, epicuticular wax amount, stomatal density) and leaf fluorescence were measured. Ozone flux into the leaves was calculated on a stomatal conductance basis. Among the measured parameters, only stomatal density and wax amount significantly differed between the sites. This response might indicate an adaptation to air pollutants, of which tropospheric ozone might be of the greatest importance

The results showed that ozone specific symptoms can be detected in the needles of mature spruces in the southern Sweden with AOT40 considerably lower than the present critical level of 10 000 nl 1sup-1. The results could be expressed as an index per tree and needle generations, but also as a single value for forest stand allowing statistical tests. The results from this study support the general hypothesis and results from exposure and field studies that low nutrient concentrations or nutrient deficiency modifies the ozone response in plants. The results also show that together with low nutrition status ozone may affect the hardiness status of the needles

The study aims at assessing response patterns to chronic O3 exposure in adult forest trees, examining physiological and structural responses at the organ and whole-tree level for consistency by means of biochemical and ecophysiological analyses. Through comparison between the two O3 regimes, conclusions can be drawn about processes, which are at risk or already affected by ozone under the unchanged, prevailing stand conditions. Responses are related to the O3 influx into leaves as a measure of the physiologically effective O3 dose and cross-compared with the AOT 40 concept which is being validated on the two O3 fluxes and AOT40-related responses in biochemical, ecophysiological and structural tree parameters