Urban ecosystems and remnant habitat 'islands' therein, provide important strongholds for many wildlife species including those of conservation significance. However, the persistence of these habitats can be undermined if their structure and function are too severely disrupted. Urban wetlands, specifically, are usually degraded by a monoculture of invasive vegetation, disrupted hydrology, and chronic-contamination from a suite of anthropogenic pollutants. Top predators—as bioindicators—can be used to assess and monitor the health of these ecosystems. We measured eight health parameters (e.g., parasites, wounds and scars, tail loss and body condition) in a wetland top predator, the western tiger snake, Notechis scutatus occidentalis. For three years, snakes were sampled across four wetlands along an urban gradient. For each site, we used GIS software to measure the area of different landscapes and calculate an urbanisation–landscape score. Previously published research on snake contamination informed our calculations of a metal-pollution index for each site. We used generalised linear mixed models to assess the relationship between all health parameters and site variables. We found the metal-pollution index to have the most significant association with poor body condition. Although parasitism, tail loss and wounds differed among sites, none of these parameters influenced body condition. Additionally, the suite of health parameters suggested differing health status among sites; however, our measure of contemporary landscape urbanisation was never a significant predictor variable. Our results suggest that the health of wetland predators surrounding a rapidly growing city may be offset by higher levels of environmental pollution.

The present paper reports on the interrelationships of fish, parasites and the bioaccumulation of hazardous organic compounds in the Zemplínska Šírava water reservoir in eastern Slovakia, which is heavily polluted with polychlorinated biphenyls (PCBs). The concentrations of these contaminants were measured in various fish matrices (dorsal and abdominal muscle tissues, hepatopancreas, intestine wall and adipose tissue) of the freshwater bream, Abramis brama (Cyprinidae), and in its intestinal parasite Caryophyllaeus laticeps (Cestoda), which was used for the first time as a model for a PCB bioaccumulation study. Regarding the fish, the highest concentrations of PCBs were found in the intestine, followed by hepatopancreas and muscle tissues. The amounts of PCBs were higher in abdominal muscles than in their dorsal parts. Concentrations of ∑PCBs above the limits set by European regulations were detected in both muscle parts in the fish, confirming the persistent unfavorable conditions in this locality and high risk for biota and humans. Based on bioconcentration factor values (BCFs), PCBs reached much higher levels in cestodes compared to bream matrices. Some significant differences in PCB amounts between infected and uninfected bream were determined. Fulton's condition factor (CF) significantly differed in infected and non-infected fish (p ˂ 0.05), with CF values surprisingly lower in fish free of parasites compared to parasitized fish, which suggests a “mutualistic” relationship between the parasite and its host.

The adaptive fitness of insect species can be shaped by how males and females respond, both physiologically and behaviorally, to environmental challenges, such as pesticide exposure. In parasitoid wasps, most toxicological investigations focus only on female responses (e.g., survival and especially parasitism abilities), leaving the male contributions to adaptive fitness (survival, locomotion, mate search) poorly investigated. Here, we evaluated the toxicity of the spinosyn insecticide spinosad against the South American fruit fly, Anastrepha fraterculus, and we used the parasitoid wasp Diachasmimorpha longicaudata (Ashmead) to evaluate whether sex-linked locomotory and physiological responses would influence the susceptibility of these organisms to spinosad. Our results revealed that D. longicaudata males were significantly more susceptible (median lethal time (LT₅₀) = 24 h) to spinosad than D. longicaudata females (LT₅₀ = 120 h), which may reflect the differences in their locomotory and physiological (e.g., respiratory) responses to mitigate insecticide exposure. Compared to D. longicaudata females, male wasps were lighter (P < 0.001), walked for longer distances (P < 0.001) and periods (P < 0.001), and exhibited higher sensilla densities in their tarsi (P = 0.008), which may facilitate their intoxication with the insecticide. These findings indicate that male parasitoids should not be exempt from insecticide selectivity tests, as these organisms can be significantly more affected by such environmental challenges than their female conspecifics.

Wild organisms are increasingly exposed to multiple anthropogenic and natural stressors that can interact in complex ways and lead to unexpected effects. In aquatic ecosystems, contamination by trace metals has deleterious effects on fish health and commonly co-occurs with pathogens, which affect similar physiological and behavioral traits. However, the combined effects of metal contamination and parasitism are still poorly known. In addition, the sensitivity to multiple stressors could be highly variable among different fish populations depending on their evolutionary history, but this intraspecific variability is rarely taken into account in existing ecotoxicological studies. Here, we investigated i) the interactive effects of metal contamination (i.e., realistic mixture of Cd, Cu and Zn) and immune challenge mimicking a parasite attack on fish health across biological levels. In addition, we compared ii) the physiological and behavioral responses among five populations of gudgeon fish (Gobio occitaniae) having evolved along a gradient of metal contamination. Results show that single stressors exposure resulted in an increase of immune defenses and oxidative stress at the expense of body mass (contamination) or fish swimming activity (immune challenge). Multiple stressors had fewer interactive effects than expected, especially on physiological traits, but mainly resulted in antagonistic effects on fish swimming activity. Indeed, the immune challenge modified or inhibited the effects of contamination on fish behavior in most populations, suggesting that multiple stressors could reduce behavioral plasticity. Interestingly, the effects of stressors were highly variable among populations, with lower deleterious effects of metal contamination in populations from highly contaminated environments, although the underlying evolutionary mechanisms remain to be investigated. This study highlights the importance of considering multiple stressors effects and intraspecific variability of sensitivity to refine our ability to predict the effects of environmental contaminants on aquatic wildlife.

Estuarine clams Scrobicularia plana were sampled from 108 intertidal locations around the English Channel and adjacent areas. Although S. plana is believed to be a strict gonochorist, 58% of the populations sampled included intersexed individuals (described as male clams exhibiting ovotestis). Over the entire region, on average, 8.6% of male clams exhibited intersex, although proportions of affected males ranged from 0% to 53% depending on location. The severity of intersex was assessed using a simple classification scale, with the majority of individuals showing low levels of impact. Sex ratios were significantly skewed at some sites. There were no significant relationships between incidence or severity of intersex; or with size or parasitism of individual clams. Intersex in S. plana is a useful tool to assess endocrine disruptive effects in estuaries, although mechanisms of impact and causative agents remain uncertain.

Cotesia flavipes is an important hymenopteran larval parasitoid that belongs to the family Braconidae. Its usage in pest management strategies is promising due to its parasitic impact on the larval stage of lepidopteran pests. The current investigation aims to determine the optimal host age for the parasitoid’s mass proliferation and augmentative releases. The experiments showed that the female C. flavipes parasitizes all larval age groups of Sesamia inferens. Among all the larval ages, C. flavipes preferred second to third instars for parasitism during the spring (up to 90%) and kharif (up to 80%) seasons. There was no substantial difference in the development period between stinging, cocoon production, and the adult emergence of parasitoids. The age of the host has a substantial impact on adult longevity, with females taking longer than males. Thus, larval instars (second and third) are also recommended for high-quality mass-rearing larval parasitoids, especially C. flavipes, due to their strong parasitism and high net reproductive rate. Therefore, the second and third instars of S. inferens will recommend the mass rearing of C. flavipes and the release of these parasitoids in the field as a successful bio-control program.

The ectoparasitoid Tamarixia triozae is a promising biological control agent of the tomato psyllid, Bactericera cockerelli, based on its high parasitism rates on different crops. The parasitism, host feeding, and transgenerational effects (in terms of sex ratio) of T. triozae females exposed to three insecticides (soybean oil, imidacloprid, and abamectin) as eggs, larvae, and pupae were evaluated when a mixture of second, third, fourth, and fifth instars of the host B. cockerelli was offered. The concentrations bioassayed of each insecticide corresponded to the minimum field-registered concentration [MiFRC] and one-half the MiFRC. No parasitism of B. cockerelli second instars was recorded when parasitoid’s females were exposed in any of the three immature stages to any of the insecticides. In contrast, in some cases, parasitism of T. triozae females treated as eggs, larvae, or pupae with soybean oil and imidacloprid was reduced in third, fourth, or fifth instar. In most cases, the host feeding was reduced in second and third instar of the host B. cockerelli when T. triozae females were treated as eggs, larvae, or pupae. Any insecticide modified the sex ratio in the F2 generation. In conclusion, both parasitism and host feeding were affected by the insecticides depending on the concentration and on the nymphal instar of the host B. cockerelli offered.