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On the bonding of manganese, copper and cadmium to peptides of the xylem sap of plant roots.
1991
Przemeck E. | Haase N.U.
Significance of chemical affinity on metal subcellular distribution in yellow perch (Perca flavescens) livers from Lake Saint-Pierre (QUEBEC, Canada)
2022
Desjardins, Kimberley | Khadra, Mélissa | Caron, Antoine | Ponton, Dominic E. | Rosabal, Maikel | Amyot, Marc
The subcellular partitioning approach provides useful information on the location of metals within cells and is often used on organisms with high levels of bioaccumulation to establish relationships between the internal concentration and the potential toxicity of metals. Relatively little is known about the subcellular partitioning of metals in wild fish with low bioaccumulation levels in comparison with those from higher contaminated areas. This study aims to examine the subcellular partitioning of various metals considering their chemical affinity and essentiality at relatively low contamination levels. Class A (Y, Sr), class B (Cu, Cd, MeHg), and borderline (Fe, Mn) metal concentrations were measured in livers and subcellular fractions of yellow perch (n = 21) collected in Lake Saint-Pierre, QC, Canada. The results showed that all metals, apart from MeHg, were distributed among subcellular fractions according to their chemical affinity. More than 60% of Y, Sr, Fe, and Mn were found in the metal-sensitive fractions. Cd and Cu were largely associated with the metallothionein-like proteins and peptides (60% and 67% respectively) whereas MeHg was found mainly in the metal-sensitive fractions (86%). In addition, the difference between the subcellular distribution of Cu and other essential metals like Fe and Mn denotes that, although the essentiality of some metals is a determinant of their subcellular distribution, the chemical affinity of metals is also a key driver. The similarity of the subcellular partitioning results with previous studies on yellow perch and other fish species from higher contaminated areas supports the idea that metals are distributed in the cellular environment according to their chemical properties regardless of the bioaccumulation gradient.
Show more [+] Less [-]Shedding light on toxicity of SARS-CoV-2 peptides in aquatic biota: A study involving neotropical mosquito larvae (Diptera: Culicidae)
2021
Mendonça-Gomes, Juliana Moreira | Charlie-Silva, Ives | Guimarães, Abraão Tiago Batista | Estrela, Fernanda Neves | Calmon, Marilia Freitas | Miceli, Rafael Nava | Sanches, Paulo R.S. | Bittar, Cíntia | Rahal, Paula | Cilli, Eduardo M. | Ahmed, Mohamed Ahmed Ibrahim | Vogel, Christoph F.A. | Malafaia, Guilherme
Knowledge about how the COVID-19 pandemic can affect aquatic wildlife is still extremely limited, and no effect of SARS-CoV-2 or its structural constituents on invertebrate models has been reported so far. Thus, we investigated the presence of the 2019-new coronavirus in different urban wastewater samples and, later, evaluated the behavioral and biochemical effects of the exposure of Culex quinquefasciatus larvae to two SARS-CoV-2 spike protein peptides (PSPD-2002 and PSPD-2003) synthesized in our laboratory. Initially, our results show the contamination of wastewater by the new coronavirus, via RT-qPCR on the viral N1 gene. On the other hand, our study shows that short-term exposure (48 h) to a low concentration (40 μg/L) of the synthesized peptides induced changes in the locomotor and the olfactory-driven behavior of the C. quinquefascitus larvae, which were associated with increased production of ROS and AChE activity (cholinesterase effect). To our knowledge, this is the first study that reports the indirect effects of the COVID-19 pandemic on the larval phase of a freshwater invertebrate species. The results raise concerns at the ecological level where the observed biological effects may lead to drastic consequences.
Show more [+] Less [-]Production and sedimentation of peptide toxins nodularin-R and microcystin-LR in the northern Baltic Sea
2009
Kankaanpää, Harri T. | Sjövall, Olli | Huttunen, Maija | Olin, Miikka | Karlsson, Krister | Hyvärinen, Kirsi | Sneitz, Laura | Härkönen, Janne | Sipiä, Vesa O. | Meriluoto, Jussi A.O.
This seven-year survey was primarily targeted to quantification of production of nodularin-R (NOD-R), a cyclic pentapeptide hepatotoxin, in Baltic Sea cyanobacteria waterblooms. Additionally, NOD-R and microcystin-LR (MC-LR; a cyclic heptapeptide toxin) sedimentation rates and NOD-R sediment storage were estimated. NOD-R production (70-2450 μg m⁻³; 1 kg km⁻² per season) and sedimentation rates (particles; 0.03-5.7 μg m⁻² d⁻¹; 0.3 kg km⁻² per season) were highly variable over space and time. Cell numbers of Nodularia spumigena did not correlate with NOD-R quantities. Dissolved NOD-R comprised 57-100% of total NOD-R in the predominantly senescent, low-intensity phytoplankton blooms and seston. Unprecedentedly intensive MC-LR sedimentation (0.56 μg m⁻² d⁻¹) occurred in 2004. Hepatotoxin sedimentation rates highly exceeded those of anthropogenic xenobiotics. NOD-R storage in surficial sediments was 0.4-20 μg kg⁻¹ (0.1 kg km⁻²). Loss of NOD-R within the chain consisting of phytoplankton, seston and soft sediments seemed very effective. Production of nodularin-R in pelagic Baltic Sea takes place in a scale of up to kilograms per square kilometre.
Show more [+] Less [-]The spectral characteristics and cadmium complexation of soil dissolved organic matter in a wide range of forest lands
2022
Zhang, Xiaoqing | Li, Ya | Ye, Jun | Chen, Zhihua | Ren, Dajun | Zhang, Shuqin
The quality and quantity of dissolved organic matter (DOM) greatly controls the fate of heavy metals. The characteristics of DOM and its interaction with metals are essential for the metal ecological risk assessment of soils. In this study, the DOM spectral characteristics of representative forest soils and the complex capacities between fluorescent DOM components and cadmium (Cd) were analyzed. Functional groups, such as carboxylic acids, alcohols and phenols, were determined by FT-IR analysis. Chromophoric DOM, fluorescent DOM and dissolved organic carbon (DOC) concentrations exhibited strong correlations with each other, indicating that variations of DOC could be well explained by Chromophoric DOM or fluorescent DOM due to high correlation coefficients. The spectral slope ratio was in the range of 0.85–5.90, implying an abundance of heavy macromolecular humic acids, peptides, and polycondensates. The absorbance spectral at 254 nm (SUVA₂₅₄) strongly correlated with SUVA₂₆₀ (r = 0.992, P < 0.01), indicating that hydrophobicity closely related with aromatic structure, and aromatic groups could be broadly hydrophobic. Fluorescence indices were from 1.62 to 2.21 and biological index values ranged from 0.54 to 1.14, where the DOM was mainly sourced from mixed terrestrial and autogenous inputs in most sites. Four universal fluorescence components were identified and characterized by fluorescence EEM-PARAFAC, including two humic-like (components 1 and 2), one tyrosine-like (components 3) and one fulvic-like (components 4) component. Both components 3 and 4 showed fluorescence quenching with increasing Cd concentrations, while components 1 and 2 had no evident change in fluorescence intensity. The logK₃ and logK₄ values ranged from 4.41 to 5.29 and 4.71 to 5.54, respectively, with most logK values of component 3 for Cd binding being smaller than that of component 4, thus, indicating that the fulvic acid substances exhibited stronger and more stable interactions with Cd than protein-like components.
Show more [+] Less [-]The inflammation response and risk associated with aflatoxin B1 contamination was minimized by insect peptide CopA3 treatment and act towards the beneficial health outcomes
2021
Dey, Debasish Kumar | Chang, Sukkum Ngullie | Kang, S. C. (Sun Chul)
This study focused on the possible chemo-preventive effects of insect peptide CopA3 on normal human colon cells against the inflammation induced by the toxic environmental pollutant aflatoxin B1 (AFB1). In the study, we used CCD 841 CoN normal human colon cells to investigate the cytotoxic effect induced by AFB1 and elucidated the negative impact of AFB1 exposure on the cell cycle progression. Further, we also carried out the in-vivo experiment, where male BALB/c mice were administrated with AFB1 to induce inflammation associated cancer like phenotype and the dietary effect of CopA3 was evaluated on the early stages of AFB1-induced hepatotoxicity and inflammation in colon tissues. At the initiation stage, CopA3 was given along with water, which significantly decreased the inflammation in the liver and colon of AFB1 exposed mice model. Mice that received CopA3 alone showed enhanced activity of several antioxidant enzymes. In the post treatment stage, the CopA3 dosage remarkably increased the Ki-67 protein expression, indicating the enhancement in cell proliferation event and increased the number of apoptotic cells in colonic crypts, suggesting the capability of CopA3 treatment towards the epithelial cell turnover. Thus, CopA3 treatment shows its potential to inhibit the development of the early stages of AFB1-induced colon inflammation and hepatotoxicity in mice by inhibiting the DNA synthesis of the damaged and inflammatory cell and induced apoptosis for the clearance of damaged cells. Collectively, the results of this study suggest that CopA3 treatment may play a protective role against the mycotoxin induced inflammation.
Show more [+] Less [-]Identification of novel paraben-binding peptides using phage display
2020
Lee, Jaewoong | Kim, Ji Hun | Kim, Bit-Na | Kim, Taehwan | Kim, Sunchang | Cho, Byung-Kwan | Kim, Yang-Hoon | Min, Jiho
Parabens are alkyl esters of 4-hydroxybenzoic acid, which is derived from a family of synthetic esters of p-hydroxybenzoic acid. Among all the kinds of paraben, two parabens (methyl paraben, MP; and n-propyl paraben, PP) are the most generally used as preservatives in personal care products, such as cosmetics, pharmaceuticals, and food also, and are often presented together. However, a number of studies have reported that the toxicity of parabens affects the water environment, and human as well. This study utilized M13 phage display technology to provide easy, efficient, and relatively inexpensive methods to identify peptides that bind to MP and PP, respectively, to remove in wastewater. At first, biopanning was performed, to sort MP and PP specific binding phages, and three cases of experiment, including negative control (NC), which could sort unspecific binding phage, were conducted at the same time. Phage binding affinity tests were substituted by concentration reduction using antibody conjugated magnetic beads, and paraben concentration was measured by HPLC. Analysis showed that the MP concentration reduction of 38% was the highest in M4 phage, while the PP concentration reduction of 44% was the highest in P3 phage. We successfully screened two peptides specific to MP and PP, namely, MP4 and PP3, respectively; the results showed that the MP concentration reduction in MP4 was the highest at 44%, and the PP concentration reduction in PP3 was the highest at 39%, and their specificity was measured by the capture rate between target and control. In conclusion, the phage display technique shows applicability to the removal of parabens in water; furthermore, it also shows the possibility of the detection or removal of other chemicals.
Show more [+] Less [-]Evaluated serum perfluoroalkyl acids and their relationships with the incidence of rheumatoid arthritis in the general population in Hangzhou, China
2022
Qu, Jianli | Zhao, Yun | Zhang, Li | Hu, Shilei | Liao, Kaizhen | Zhao, Meirong | Wu, Pengfei | Jin, Hangbiao
Perfluoroalkyl acids (PFAAs) are widely present in human blood, and have many toxic effects on humans. However, effects of PFAA exposure on the risk of rheumatic immune diseases are limited. In the present study, occurrence of 7 PFAAs, including perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), perfluoroundecanoate (PFUnA), perfluorododecanoate (PFDoA), and perfluorotrdecanoate (PFTrA), were measured in serum samples from 156 healthy people (controls) and 156 rheumatoid arthritis (RA) cases living in Hangzhou, China. We also investigated the relationships among cumulative PFAA levels in serum, some immune markers, and the incidence of RA. The results showed that PFOA (6.1 and 11.8 ng/mL) had the highest mean serum concentrations, followed by PFOS (3.2 and 3.4 ng/mL) and PFDA (0.86 and 2.6 ng/mL), in both controls and RA cases. Cumulative exposure to PFOA in the study population were positively correlated with the levels of rheumatoid factors (rₛ = 0.69, p < 0.01) and anti-cyclic citrullinated peptide antibody (rₛ = 0.56, p < 0.05). Moreover, significant associations of PFOA concentrations with odds ratios (OR) of RA (OR = 1.998, confidence interval (CI): 1.623, 2.361, p = 0.01) were found by adjusting for various covariates. The crude and adjusted OR for RA was respective 1.385 (95% CI: 1.270, 1.510, p = 0.04) and 1.381 (95% CI: 0.972, 1.658, p = 0.06) for a unit increase in serum PFOS levels, but the adjusted results were not significant. Overall, this case-control study found that human serum PFOA concentrations were positively correlated with RF and ACPA levels.
Show more [+] Less [-]Proteogenomics identification of TBBPA degraders in anaerobic bioreactor
2022
Macêdo, Williane Vieira | Poulsen, Jan Struckmann | Zaiat, Marcelo | Nielsen, Jeppe Lund
Tetrabromobisphenol A (TBBPA) is the most used flame retardant worldwide and has become a threat to aquatic ecosystems. Previous research into the degradation of this micropollutant in anaerobic bioreactors has suggested several identities of putative TBBPA degraders. However, the organisms actively degrading TBBPA under in situ conditions have so far not been identified. Protein-stable isotope probing (protein-SIP) has become a cutting-edge technique in microbial ecology for enabling the link between identity and function under in situ conditions. Therefore, it was hypothesized that combining protein-based stable isotope probing with metagenomics could be used to identify and provide genomic insight into the TBBPA-degrading organisms. The identified ¹³C-labelled peptides were found to belong to organisms affiliated to Phytobacter, Clostridium, Sporolactobacillus, and Klebsilla genera. The functional classification of identified labelled peptides revealed that TBBPA is not only transformed by cometabolic reactions, but also assimilated into the biomass. By application of the proteogenomics with labelled micropollutants (protein-SIP) and metagenome-assembled genomes, it was possible to extend the current perspective of the diversity of TBBPA degraders in wastewater and predict putative TBBPA degradation pathways. The study provides a link to the active TBBPA degraders and which organisms to favor for optimized biodegradation.
Show more [+] Less [-]Metagenomic insights into the antibiotic resistome in freshwater and seawater from an Antarctic ice-free area
2022
Zhang, Tao | Ji, Zhongqiang | Li, Jun | Yu, Liyan
The comprehensive profiles of antibiotic resistance genes (ARGs) in the Antarctic water environments and their potential health risks are not well understood. The present study characterized the bacterial community compositions and ARG profiles of freshwater (11 samples) and seawater (28 samples) around the Fildes Region (an ice-free area in Antarctica) using a shotgun metagenomic sequencing approach for the first time. There were significant differences in the compositions of the bacterial community and ARG profiles between freshwater and seawater. In the 39 water samples, 114 ARG subtypes belonging to 15 ARG types were detectable. In freshwater, the dominant ARGs were related to multidrug and rifamycin resistance. In seawater, the dominant ARGs were related to peptide, multidrug, and beta-lactam resistance. Both the bacterial community compositions and ARG profiles were significantly related to certain physicochemical properties (e.g., pH, salinity, NO₃⁻). Procrustes analysis revealed a significant correlation between the bacterial community compositions and ARG profiles of freshwater and seawater samples. A total of 31 metagenome-assembled genomes (MAGs) carrying 35 ARG subtypes were obtained and identified. The results will contribute to a better evaluation of the ARG contamination in relation to human health in the Antarctic aquatic environments.
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