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Differences between a deciduous and a conifer tree species in gaseous and particulate emissions from biomass burning
2018
Pallozzi, Emanuele | Lusini, Ilaria | Cherubini, Lucia | Hajiaghayeva, Ramilla A. | Ciccioli, Paolo | Calfapietra, Carlo
In the Mediterranean ecosystem, wildfires are very frequent and the predicted future with a probable increase of fires could drastically modify the vegetation scenarios. Vegetation fires are an important source of gases and primary emissions of fine carbonaceous particles in the atmosphere. In this paper, we present gaseous and particulate emissions data from the combustion of different plant tissues (needles/leaves, branches and needle/leaf litter), obtained from one conifer (Pinus halepensis) and one deciduous broadleaf tree (Quercus pubescens). Both species are commonly found throughout the Mediterranean area, often subject to wildfires. Experiments were carried out in a combustion chamber continuously sampling emissions throughout the different phases of a fire (pre-ignition, flaming and smoldering). We identified and quantified 83 volatile organic compounds including important carcinogens that can affect human health. CO and CO₂ were the main gaseous species emitted, benzene and toluene were the dominant aromatic hydrocarbons, methyl-vinyl-ketone and methyl-ethyl-ketone were the most abundant measured oxygenated volatile organic compounds. CO₂ and methane emissions peaked during the flaming phase, while the peak of CO emissions occurred during the smoldering phase. Overall, needle/leaf combustion released a greater amount of volatile organic compounds into the atmosphere than the combustion of branches and litter. There were few differences between emissions from the combustion of the two tree species, except for some compounds. The combustion of P. halepensis released a great amount of monoterpenes as α-pinene, β-pinene, p-cymene, sabinene, 3-carene, terpinolene and camphene that are not emitted from the combustion of Q. pubescens. The combustion of branches showed the longest duration of flaming and peak of temperature. Data presented appear crucial for modeling with the intent of understanding the loss of C during different phases of fire and how different typologies of biomass can affect wildfires and their speciation emissions profile.
Show more [+] Less [-]Potential metabolism of pharmaceuticals in radish: Comparison of in vivo and in vitro exposure
2018
Li, Yuanbo | Chuang, Ya-Hui | Sallach, J Brett | Zhang, Wei | Boyd, Stephen A. | Li, Hui
Metabolism of pharmaceuticals in plants is important to evaluate their fate and accumulation in vegetables, and subsequently the risks to human health. However, limited knowledge is available to evaluate metabolism of pharmaceuticals in plants due to the lack of appropriate research approaches. In this study, radish was selected as a model plant to investigate metabolism of pharmaceuticals in intact plants (in vivo) growing in hydroponic solution and in plant tissue enzyme extracts (in vitro). For caffeine, six phase-I demethylation metabolites identified in the intact radish plant were also found in the plant enzyme extracts. After 7 days of in vivo exposure, the amount of the identified metabolites was about 5.4 times greater than the parent compound caffeine in radish roots. Furthermore, the metabolism potential of fifteen pharmaceuticals in radish was evaluated on the basis of mass balance. After 7 days of hydroponic exposure, oxytetracycline, trimethoprim, carbamazepine, lincomycin, monensin and tylosin manifested relatively less extent of metabolism with the mass recoveries ranging from 52.3 to 78.2%. In contrast, 17 β-estradiol, sulfamethoxazole, sulfadiazine, estrone, triclosan, acetaminophen, caffeine, carbadox and lamotrigine underwent extensive metabolism with only 3.0 to 32.1% of the parent compound recovered. In the in vitro system, 17 β-estradiol, estrone, triclosan, oxytetracycline, acetaminophen, sulfadiazine and sulfamethoxazole were readily metabolized in radish root enzyme extracts with 1.8 to 34.0% remaining after 96-h exposure. While in the leaf enzyme extracts, only triclosan was rapidly metabolized with 49.2% remaining, and others pharmaceuticals were ≥60%, indicating that the varying extents of metabolism occurred in different plant parts. This study highlights the importance of pharmaceutical metabolism in plants, and suggests that plant tissue enzyme extracts could serve as an alternative tool to assess pharmaceutical metabolism in plants.
Show more [+] Less [-]Uptake, translocation and biotransformation of N-ethyl perfluorooctanesulfonamide (N-EtFOSA) by hydroponically grown plants
2018
Zhao, Shuyan | Zhou, Tao | Zhu, Lingyan | Wang, Bohui | Li, Ze | Yang, Liping | Liu, Lifen
N-ethyl perfluorooctane sulfonamide (N-EtFOSA) is an important perfluorooctanesulfonate (PFOS) precursor (PreFOS) which is used in sulfluramid. The present work studied the uptake, translocation and metabolism of N-EtFOSA in wheat (Triticum aestivum L.), soybean (Glycine max L. Merrill) and pumpkin (Cucurbita maxima L.) by hydroponic exposure. Except for parent N-EtFOSA, its metabolites of perfluorooctane sulfonamide acetate (FOSAA), perfluorooctane sulfonamide (PFOSA), PFOS, perfluorohexane sulfonate (PFHxS) and perfluorobutane sulfonate (PFBS) were detected in the roots and shoots of all the three plant species examined. This suggested that plant roots could take up N-EtFOSA from solutions efficiently, and translocate to shoots. A positive correlation was found between root concentration factors (RCFs) of N-EtFOSA and root lipid content. Much higher proportion of N-EtFOSA transformation products in plant tissues than in the solutions suggested that N-EtFOSA could be in vivo metabolized in plant roots and shoots to FOSAA, PFOSA and PFOS, and other additional shorter-chain perfluoroalkane sulfonates (PFSAs), including PFHxS and PFBS. The results suggested that plants had biotransformation pathways to N-EtFOSA that were different than those from microorganisms and animals. This study provides important information about the uptake and metabolism of PreFOSs in plants.
Show more [+] Less [-]Stable isotope fractionation during uptake and translocation of cadmium by tolerant Ricinus communis and hyperaccumulator Solanum nigrum as influenced by EDTA
2018
Wei, Rongfei | Guo, Qingjun | Yu, Guirui | Kong, Jing | Li, Siliang | Song, Zhaoliang | Hu, Jian | Tian, Liyan | Han, Xiaokun | Okoli, Chukwunonso Peter
The isotopic fractionation could contribute to understanding the Cd accumulation mechanisms in plant species. However, there are few of systematical investigations with regards to the Cd isotope fractionation in hyperaccumulator plants. The Cd tolerant Ricinus communis and hyperaccumulator Solanum nigrum were cultivated in nutrient solutions with varying Cd and EDTA concentrations. Cd isotope ratios were determined in the solution, root, stem and leaf. The two investigated plants were systematically enriched in light isotopes relative to their solutions (Δ114/110Cdplant-solution = −0.64‰ to −0.29‰ for R. communis and −0.84‰ to −0.31‰ for S. nigrum). Cd isotopes were markedly fractionated among the plant tissues. For both plant species, an enrichment in light Cd isotopes from solution to root was noted, followed by a slight depletion in light Cd isotopes from root to shoot. Noticeably, the chelation process has caused lighter Cd isotope enrichment in the root of R. communis and S. nigrum. Further, the good fits between △114/110Cdroot-plant and ln Froot (or between △114/110Cdshoot-plant and ln Fshoot) indicate that Cd isotopic signatures can be used to study Cd transportation during the metabolic process of plants. This study suggests that knowledge of the Cd isotope ratios could also provide new tool for identifying the Cd-avoiding crop cultivars.
Show more [+] Less [-]Toxicity assessment of metal oxide nano-pollutants on tomato (Solanum lycopersicon): A study on growth dynamics and plant cell death
2018
The present study for the first time demonstrated the interactions of metal oxide (MO) nano-pollutants (CuO and Al2O3-NPs) with tissues and cellular DNA of tomato plants grown in soil sand: silt: clay (667:190:143) and Hoagland-hydroponic system and assessed the hazardous effects of NPs on cell physiology and biochemistry. Results of SEM equipped with EDX revealed attachment of variably shaped CuO-NPs (18 nm) and Al2O3-NPs (21 nm) on roots, and internalization followed by translocation in plants by ICP-MS and TEM. Significant variations in foliage surface area, chlorophyll, proteins, LPO, and antioxidant enzymes were recorded. Roots and shoots accumulated 225.8 ± 8.9 and 70.5 ± 4 μgAl g−1 DW, whereas Cu accumulation was 341.6 ± 14.3 (roots) and 146.9 ± 8.1 μg g−1 DW (shoots) which was significant (p ≤ 0.0005) as compared to control. The total soluble protein content in roots, shoots, and leaves collected from Al2O3-NPs treated plants increased by 120, 80, and 132%, respectively while in CuO-NPs treatments, the increase was 68 (roots), 36 (shoots), and 86% (leaves) over control. The level of antioxidant enzymes in plant tissues was significantly (p ≤ 0.05) higher at 2000 μg ml−1 of MONPs over control. A dose-dependent increase in reactive oxygen species (ROS), biphasic change of lower and higher fluorescence in mitochondria due to dissipation of mitochondrial membrane potential (ΔΨm) and membrane defects using propidium iodide were observed. Comparatively, CuO-NPs induced higher toxicity than Al2O3-NPs. Perceptible changes in proteins (amide-I & II), cellulose, glucose, galactose and other carbohydrates were observed under FT-IR. The binding studies with TmDNA showed fluorescence quenching of EtBr-TmDNA and acridine orange-TmDNA complex only by CuO-NPs with -ΔG and +ΔH and +ΔS values. However, Al2O3-NPs induced lesser change in TmDNA conformation. Conclusively, the results are novel in better demonstrating the mechanistic basis of nano-phyto-toxicity and are important which could be used to develop strategies for safe disposal of Al2O3-NPs and CuO-NPs.
Show more [+] Less [-]Effects of Apirolio Addition and Alfalfa and Compost Treatments on the Natural Microbial Community of a Historically PCB-Contaminated Soil
2018
Di Lenola, Martina | Barra Caracciolo, Anna | Grenni, Paola | Ancona, Valeria | Rauseo, Jasmin | Laudicina, VitoA. | Uricchio, VitoFelice | Massacci, Angelo
Polychlorinated biphenyls (PCBs) are ubiquitous and persistent organic pollutants generated exclusively from human sources and found in the environment as several congeners (e.g. Apirolio, produced in Italy and used for electrical transformers). To evaluate the ability of the natural microbial community of historically PCB-contaminated soil to transform or degrade PCBs after fresh contamination through the addition of Apirolio, a microcosm experiment was conducted in a greenhouse for approximately 8 months. Compost and Medicago sativa (alfalfa) were additionally used in the microcosms to stimulate microbial PCB degradation. Chemical analyses were performed to evaluate PCB concentrations in the soil and plant tissue. Changes in the microbial community under the different experimental conditions were evaluated in terms of total abundance, viability, diversity, and activity. Interestingly, the addition of Apirolio did not negatively affect the microbial community but did stimulate the degradation of the freshly added PCBs. The plant and compost co-presence did not substantially increase PCB degradation, but it increased the microbial abundance and activity and the occurrence of α-Proteobacteria and fungi.
Show more [+] Less [-]Restoration of Vegetation in Relation to Soil Properties of Spoil Heap Heavily Contaminated with Heavy Metals
2018
Pająk, Marek | Błońska, Ewa | Szostak, Marta | Gąsiorek, Michał | Pietrzykowski, Marcin | Urban, Otmar | Derbis, Piotr
The main objectives of our study were to evaluate soil contamination on a zinc-lead spoil heap in the Upper Silesian Industrial Region in southern Poland using pollution indices, and to investigate the relation between soil properties and the natural succession of vegetation. Organic carbon and nitrogen, pH, soil texture, base cations, and heavy metal content were analyzed in soil samples at depths of 0–15 cm below the organic horizon over a regular grid of 14 sampling plots. The contents of Zn, Pb, and Cd exceeded by several times the acceptable thresholds. Measurements of soil enzyme activity were used to evaluate the progress of vegetation development in relation to soil chemical properties. The results indicate that heavy metals had a significant impact on soil enzyme activity and the development of vegetation cover. High contents of Pb and Cd reduced enzyme activity, while this activity increased with increasing amounts of soil organic matter. Further, the accumulative capacities of heavy metals in needles of Scots pine (Pinus sylvestris L.) and aboveground biomass of bladder campion (Silene vulgaris (Moench) Garcke) were examined. A high accumulation of Zn, Pb, and Cd in the aboveground tissues of S. vulgaris indicated an unusual tolerance of this species to heavy metals and the possibility of using this species in phytoremediation of post-industrial sites.
Show more [+] Less [-]Foliar Application of Iron (Fe) Improved the Antioxidant Defense and Cd Accumulation Potential of Ricinus communis Under Hydroponic Condition
2018
Ullah, Sana | Hadi, Fazal | Ali, Nasir | K̲h̲ān, Sikandar
Heavy metal-polluted water has become a problem for sustainable environment, agriculture, and human health. Phyto-accumulation is an eco-friendly technique for decontamination of metal-polluted water and soil. The efficiency of phyto-accumulation and rhizo-filtration can be enhanced by the application of certain nutrients to accumulator plants. In this study, we focused on the role of iron (Fe) in rhizo-filtration and phyto-accumulation of cadmium (Cd) from polluted water/media, using Ricinus communis plant. Medium was contaminated with 10 ppm Cd while Fe (2.50, 5.00, and 7.50 ppm) was applied both as foliar spray and medium addition separately. Accumulation of Cd and concentrations of soluble proline, phenolic compounds, and chlorophylls were measured in plant tissues. Addition of Fe into media significantly increased biomass in the plants but decreased Cd absorption by roots and its accumulation in other tissues of the plants. Foliar application of Fe, especially 7.5 ppm, significantly increased biomass as well as accumulation of Cd in tissues of the plants. Contents of soluble proline (41.88 ± 3.56 ppm) and phenolics (171.00 ± 4.98 ppm) in leaves were highly increased by foliar spray of 7.5 ppm Fe on the plants. On the other hand, highest concentrations of free proline (67.00 ± 2.00 ppm) and total phenolics (82.67 ± 2.52 ppm) in plant roots were observed in 7.5 ppm Fe added to media and as foliar spray, respectively. Strong correlations were observed between phenolics content in roots and leaves with Cd accumulation after foliar application of 7.5 ppm Fe.
Show more [+] Less [-]Assessment of chromium hyper-accumulative behaviour using biochemical analytical techniques of greenhouse cultivated Sonchus asper on tannery waste dump site soils
2018
Nirola, Ramkrishna | Biswas, Bhabananda | Megharaj, Mallavarapu | Subramanian, Avudainayagam | Thavamani, Palanisami | Aryal, Rupak | Saint, Christopher
Keeping the sources of pollution such as chromium (Cr) under a safe limit is a daunting challenge due to the negative impact of heavy metal bioaccumulation in vegetation and the concomitant human health exposure. We took a closer look at Sonchus asper by cultivating in the green house. It resulted in 80% germination when cultivated over nine different soils collected from the tannery dump site. The biochemical analytical techniques such as mass spectrometry indicated significant bioaccumulation of Cr in the plant tissue. As per the ICP-MS analysis, this annual herb resulted in the accumulation of 601 mg kg⁻¹ of total Cr with 212 mg kg⁻¹ in its shoot from soil samples containing up to 41 mg kg⁻¹ of hexavalent Cr. The energy dispersive X-ray (EDX) spectroscopy of S. asper revealed a higher level of S element indicating a sulfate-Cr binding relation. Elevated content of Cr in soil (73,721 ± 65 mg kg⁻¹) caused biochemical changes in the shoot of S. asper as indicated by the disappearance of Fourier transform infrared spectroscopy (FTIR) bands at 935 and 872 cm⁻¹ and further revealing aliphatic –CH₂ appearing as anti-symmetry νₐ(CH₂) and symmetric vibration νₛ(CH₂) at the band of 2920 and 2850 cm⁻¹, respectively.
Show more [+] Less [-]Heavy metal bioaccumulation by Miscanthus sacchariflorus and its potential for removing metals from the Dongting Lake wetlands, China
2018
Yao, Xin | Niu, Yandong | Li, Youzhi | Zou, Dongsheng | Ding, Xiaohui | Bian, Hualin
Bioaccumulation of five heavy metals (Cd, Cu, Mn, Pb, and Zn) in six plant organs (panicle, leaf, stem, root, rhizome, and bud) of the emergent and perennial plant species, Miscanthus sacchariflorus, were investigated to estimate the plant’s potential for accumulating heavy metals in the wetlands of Dongting Lake. We found the highest Cd concentrations in the panicles and leaves; while the highest Cu and Mn were observed in the roots, the highest Pb in the panicles, and the highest Zn in the panicles and buds. In contrast, the lowest Cd concentrations were detected in the stem, roots, and buds; the lowest Cu concentrations in the leaves and stems; the lowest Mn concentrations in the panicles, rhizomes, and buds; the lowest Pb concentrations in the stems; and the lowest Zn concentrations in the leaves, stems, and rhizomes. Mean Cu concentration in the plant showed a positive regression coefficient with plot elevation, soil organic matter content, and soil Cu concentration, whereas it showed a negative regression coefficient with soil moisture and electrolyte leakage. Mean Mn concentration showed positive and negative regression coefficients with soil organic matter and soil moisture, respectively. Mean Pb concentration exhibited positive regression coefficient with plot elevation and soil total P concentration, and Zn concentration showed a positive regression coefficient with soil available P and total P concentrations. However, there was no significant regression coefficient between mean Cd concentration in the plant and the investigated environmental parameters. Stems and roots were the main organs involved in heavy metal accumulation from the environment. The mean quantities of heavy metals accumulated in the plant tissues were 2.2 mg Cd, 86.7 mg Cu, 290.3 mg Mn, 15.9 mg Pb, and 307 mg Zn per square meter. In the Dongting Lake wetlands, 0.7 × 10³ kg Cd, 22.9 × 10³ kg Cu, 77.5 × 10³ kg Mn, 3.1 × 10³ kg Pb, and 95.9 × 10³ kg Zn per year were accumulated by aboveground organs and removed from the lake through harvesting for paper manufacture.
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