In recent decades, industrial emissions have been reduced in many countries, which provides an opportunity for the recovery of polluted ecosystems. However, our knowledge of the rate and factors facilitating the recovery of local bird populations after pollution abatement is incomplete. Long-term (1989–2021) annual observations on nest-box populations of a passerine bird, Ficedula hypoleuca, were used to analyze temporal dynamics of breeding parameters following a 50-fold reduction of industrial emissions from the Middle Ural copper smelter (MUCS) according to pollution zone, habitat, air temperature, and breeding density. In the heavily polluted (impact) zone (1–2 km of MUCS), egg and fledgling production were strongly impaired compared to the moderately polluted (buffer zone, 4–8 km of MUCS) and unpolluted control zone (16–27 km of MUCS). During the study period, the laying date advanced along with increasing spring air temperatures. The clutch size increased in the impact zone by 26%, in the buffer zone by 10%, and in control by 5%. The number of fledglings increased in the impact zone by 102% and the buffer zone by 17%. In the most recent year (2021), mean laying date, clutch size, fledgling production, and the frequency of nests with unhatched eggs in the impact zone did not reach the control level, whereas the frequency of nests with perished chicks did not differ among zones. Breeding parameters of birds in the impact zone improved slowly, likely due to the slow recovery of habitats. We conclude that bird reproduction may require many decades to recover fully in the heavily polluted zone.

Plume chasing is cost-effective, measuring individual, on-road vehicular emissions. Whereas, wake-flow-generated turbulence results in intermittent, rapid pollutant dilution and substantial fluctuating concentrations right behind the vehicle being chased. The sampling duration is therefore one of the important factors for acquiring representative (average) concentrations, which, however, has been seldom addressed. This paper, which is based on the detailed spatio-temporal dispersion data after a heavy-duty truck calculated by large-eddy simulation (LES), examines how sampling duration affects the uncertainty of the measured concentrations in plume chasing. The tailpipe dispersion is largely driven by the jet-like flows through the vehicle underbody with approximate Gaussian concentration distribution for x ≤ 0.6h, where x is the distance after the vehicle and h the characteristic vehicle size. Thereafter for x ≥ 0.6h, the major recirculation plays an important role in near-wake pollutant transport whose concentrations are highly fluctuating and positively shewed. Plume chasing for a longer sampling duration is more favourable but is logistically impractical in busy traffic. Sampling duration, also known as averaging time in the statistical analysis, thus has a crucial role in sampling accuracy. With a longer sampling (averaging) duration, the sample mean concentration converges to the population mean, improving the sample reliability. However, this effect is less pronounced in long sampling duration. The sampling accuracy is also influenced by the locations of sampling points. For the region x > 0.6h, the sampling accuracy is degraded to a large extent. As a result, acceptable sample mean is hardly achievable. Finally, frequency analysis unveils the mechanism leading to the variance in concentration measurements which is attributed to sampling duration. Those data with frequency higher than the sampling frequency are filtered out by moving average in the statistical analyses.

Freshwater ecosystems are the main source of water for sustaining life on earth, and the biodiversity they support is the main source of valuable goods and services for human populations. Despite growing recognition of the impairment of freshwater ecosystems by micropollutant contamination, different conceptual and methodological considerations can newly be addressed to improve our understanding of the ecological impact into these ecosystems. Here, we originally combined in situ ecotoxicology and community ecology concepts to unveil the mechanisms structuring macroinvertebrate communities along a regional contamination gradient. The novelty of our study lies in the use of an innovative biomonitoring approach (measurement of metal contents in caged crustaceans) allowing to quantify and compare on a regional scale the levels of bioavailable metal contamination to which stream communities are exposed. We were hence able to identify 23 streams presenting a significant gradient of bioavailable metal contamination within the same catchment area in the South West of France, from which we also obtained data on the composition of resident macroinvertebrate communities. Analyses of structural and functional integrity of communities revealed an unexpected decoupling between taxonomic and functional diversity of communities in response to bioavailable metal contamination. We show that despite the negative impact of bioavailable metal contamination exposure on taxonomic diversity (with an average species loss of 17% in contaminated streams), functional diversity is maintained through a process of non-random species replacement by functional redundant species at the regional scale. Such unanticipated findings call for a deeper characterization of metal-tolerant communities’ ability to cope with environmental variability in multi-stressed ecosystems.

Soil polycyclic aromatic hydrocarbons (PAHs) generated from industrial processes are highly spatially heterologous, with limited quantitative studies on their main influencing factors. The present study evaluated the soil PAHs in three types of industrial parks (a petrochemical industrial park, a brominated flame retardant manufacturing park, and an e-waste dismantling park) and their surroundings. The total concentrations of 16 PAHs in the parks were 340–2.43 × 10³, 26.2–2.63 × 10³, and 394–2.01 × 10⁴ ng/g, which were significantly higher than those in the surrounding areas by 1–2 orders of magnitude, respectively. The highest soil PAH contamination was observed in the e-waste dismantling park. Nap can be considered as characteristic pollutant in the petrochemical industrial park, while Phe in the flame retardant manufacturing park and e-waste dismantling park. Low molecular weight PAHs (2–3 rings) predominated in the petrochemical industrial park (73.0%) and the surrounding area of brominated flame retardant manufacturing park (80.3%). However, high molecular weight PAHs (4–6 rings) were enriched in the other sampling sites, indicating distinct sources and determinants of soil PAHs. Source apportionment results suggested that PAHs in the parks were mainly derived from the leakage of petroleum products in the petroleum manufacturing process and pyrolysis or combustion of fossil fuels. Contrarily, the PAHs in the surrounding areas could have been derived from the historical coal combustion and traffic emissions. Source emissions, wind direction, and local topography influenced the PAH spatial distributions.

Hydrothermal carbonization is considered a powerful technology to convert sewage sludge (SS) into a valuable carbonaceous solid known as hydrochar (HC). Up to now criteria for landfill application of SS and HC are based only on physicochemical properties and levels of pollutant residues. Nevertheless, to ensure their safe environmental applications it is mandatory to develop biosensors which can provide relevant information on their toxic potential for natural ecosystems. Therefore, this study aimed to assess the suitability of a contact assay using zebrafish embryo/larvae combined with sub-lethal end-points to evaluate the hazard associated with SS and related HC exposure. A suite of biomarkers was also applied on larvae, related to detoxification and oxidative stress as the activity of Ethoxyresorufin-O-deethylase, glutathione-S-transferase, and catalase, the content of reactive oxygen species and the behavioral assay using the DanioVision™ chamber. Legacy priority pollutants were also measured either in SS and HC tested samples and in contact waters. The exposure to SS caused higher lethality compared to HC. No significant changes in the activity of oxidative stress markers was observed upon exposure to both matrices. The behavioral test showed a hypoactivity condition in larvae exposed to both SS and HC with the effects of SS stronger than HC. Chemical analysis revealed the presence of trace elements and halogenated compounds in either SS, HC. Heavy metals were also released in contact waters, while volatile hydrocarbons (C6–C10) and halogenated compounds resulted below LOD (<0.05 μ L⁻¹). Our study highlights the suitability of zebrafish embryotoxicity test, coupled with behavioral traits, as screening tool for assessing potential risks, associated with the landfill application of both SS and HC, for aquatic wildlife.

Plant-derived saponins are bioactive surfactant compounds that can solubilize organic pollutants in environmental matrices, thereby facilitating pollutant remediation. Externally applied saponin has potential to enhance total petroleum hydrocarbon (TPH) biodegradation in the root zone (rhizosphere) of wild plants, but the associated mechanisms are not well understood. For the first time, this study evaluated a triterpenoid saponin (from red ash leaves, Alphitonia excelsa) in comparison to a synthetic surfactant (Triton X-100) for their effects on plant growth and biodegradation of TPH in the rhizosphere of two native wild species (a grass, Chloris truncata, and a shrub, Hakea prostrata). The addition of Triton X-100 at the highest level (1000 mg/kg) in the polluted soil significantly hindered the plant growth (reduced plant biomass and photosynthesis) and associated rhizosphere microbial activity in both the studied plants. Therefore, TPH removal in the rhizosphere of both plant species treated with the synthetic surfactant was not enhanced (at the lower level, 500 mg/kg soil) and even slightly decreased (at the highest level) compared to that in the surfactant-free (control) treatment. By contrast, TPH removal was significantly increased with saponin application (up to 60% in C. truncata at 1000 mg/kg due to enhanced plant growth and associated rhizosphere microbial activity). No significant difference was observed between the two saponin application levels. Dehydrogenase activity positively correlated with TPH removal (p < 0.001) and thus this parameter could be used as an indicator to predict the rhizoremediation efficiency. This work indicates that saponin-amended rhizoremediation could be an environmentally friendly and effective biological approach to remediate TPH-polluted soils. It was clear that the enhanced plant growth and rhizosphere microbial activity played a crucial role in TPH rhizoremediation efficiency. The saponin-induced molecular processes that promoted plant growth and soil microbial activity in the rhizosphere warrant further studies.

Microplastic pollution affects freshwater and marine biota worldwide, microplastics occurring even inside the organisms. With highly variable effects, from physical damage to toxicity of plastic compounds, microplastics are a potential threat to the biodiversity, community composition and organisms' health. This emerging pollutant could overstress diadromous species, which are exposed to both sea and river water in their life cycle. Here we have quantified microplastics in young European eel Anguilla anguilla, a critically endangered catadromous fish, entering three rivers in southwestern Bay of Biscay. River water, sediments and seawater were also analysed for microplastics. The microplastic type was identified using Fournier-Transform Infrared spectroscopy and then searched for their hazard potential at the European Chemical Agency site. Both riverine and sea microplastic pollution were predictors of eels’ microplastic profile (types of microplastics by shape and colour): A. anguilla juveniles entering European rivers already carry some marine microplastics and acquire more from river water. Potentially hazardous plastic materials were found from eels, some of them dangerous for aquatic life following the European Chemical Agency. This confirms microplastics as a potential threat for the species. Between-rivers differences for microplastics profiles persistent over years highlight the convenience of analysing and preventing microplastics at a local spatial scale, to save diadromous species from this stressor. Since the origin of microplastics present in glass eels seems to be dual (continental + seawater), new policies should be promoted to limit the entry of microplastics in sea and river waters.

Microplastics (MPs) are an emerging pollutant and a worldwide issue. A wide variety of MPs and tyre wear particles (TWPs) are entering and spreading in the environment. TWPs can reach waterbodies through runoff, where main contributing particulate matter comes from impervious areas. In this paper, TWPs and other types of MPs that were transported with the runoff of a high populated-impervious urban area were characterised. Briefly, MPs were sampled from sediments in a stormwater detention reservoir (SDR) used for flood control of a catchment area of ∼36 km², of which 73% was impervious. The sampled SDR is located in São Paulo, the most populated city in South America. TWPs were the most common type of MPs in this SDR, accounting for 53% of the total MPs; followed by fragments (30%), fibres (9%), films (4%) and pellets (4%). In particular, MPs in the size range 0.1 mm–0.5 mm were mostly TWPs. Such a profile of MPs in the SDR is unlike what is reported in environmental compartments elsewhere. TWPs were found at levels of 2160 units/(kg sediment·km² of impervious area) and 87.8 units/(kg sediment·km street length); MP and TWP loadings are introduced here for the first time. The annual flux of MPs and TWPs were 7.8 × 10¹¹ and 4.1 × 10¹¹ units/(km²·year), respectively, and TWP emissions varied from 43.3 to 205.5 kg/day. SDRs can be sites to intercept MP pollution in urban areas. This study suggests that future research on MP monitoring in urban areas and design should consider both imperviousness and street length as important factors to normalize TWP contribution to urban pollution.

The North Sea and its coastal zones are heavily impacted by anthropogenic activities, which has resulted in significant chemical pollution ever since the beginning of the industrialization in Europe during the 19th century. In order to assess the chemical Anthropocene, natural archives, such as sediment cores, can serve as a valuable data source to reconstruct historical emission trends and to verify the effectiveness of changing environmental legislation. In this study, we investigated 90 contaminants covering inorganic and organic pollutant groups analyzed in a set of sediment cores taken in the North Seas' main sedimentation area (Skagerrak). We thereby develop a chemical pollution fingerprint that records the constant input of pollutants over time and illustrates their continued great relevance for the present. Additionally, samples were radiometrically dated and PAH and PCB levels in porewater were determined using equilibrium passive sampling. Furthermore, we elucidated the origin of lead (Pb) contamination utilizing non-traditional stable isotopic analysis. Our results reveal three main findings: 1. for all organic contaminant groups covered (PAHs, OCPs, PCBs, PBDEs and PFASs) as well as the elements lead (Pb) and titanium (Ti), determined concentrations decreased towards more recent deposited sediment. These decreasing trends could be linked to the time of introductions of restrictions and bans and therefor our results confirm, amongst possible other factors, the effectiveness of environmental legislation by revealing a successive change in contamination levels over the decades. 2. concentration trends for ΣPAH and ΣPCB measured in porewater correspond well with the ones found in sediment which suggests that this method can be a useful expansion to traditional bulk sediment analysis to determine the biologically available pollutant fraction. 3. Arsenic (As) concentrations were higher in younger sediment layers, potentially caused by emissions of corroded warfare material disposed in the study area after WW II.

No anthropogenic pollutant is more widespread in the aquatic and terrestrial environment than microplastic; however, there are large knowledge gaps regarding its origin, fate, or temporal variations in the oceans. In this study, we analyzed sediment trap material from the deep subtropical Northeast Atlantic (2000 m) in a long-term record (2003–2015) to assess the role of the deep ocean as a potential sink of microplastics. Microplastic particles were identified in all 110 analyzed samples with flux rates of 1.13–3146.81 items d⁻¹ m⁻². Calculated microplastic mass fluxes ranged between 0.10 and 1977.96 μg d⁻¹ m⁻², representing up to 8% of the particle flux. Between years, the composition of the different polymers changed significantly, dominated by polyethylene, whose amount was correlated with the lithogenic input. The correlation between polyethylene and the lithogenic fraction was attributed to an air transport pathway from northeast Africa and surrounding regions. The second most abundant polymer detected in our study was polyvinyl chloride, which is not correlated with lithogenic or biogenic particle flux fractions. Instead, we observed seasonality for polyvinyl chloride with recurring high fluxes in winter before the plankton bloom and significantly lower amounts in summer. Other polymers identified were polypropylene, polyethylene terephthalate, and lower numbers of polystyrene and polymethyl methacrylate. The average microplastic particle size for all samples and polymers was 88.44 ± 113.46 μm, with polyethylene and polyvinyl chloride having the highest proportion of small particles (<100 μm). Our findings provide first insights into temporal variations of sinking microplastics, which are crucial for understanding the fate of plastic in the oceans.