Microplastics are widely used to manufacture diverse products such as textiles, skin care products, and household products such as detergents and soaps. However, microplastic pollution and its potential health risks are raising concerns worldwide. This study characterized and determined the safety of microplastics in water and sediments obtained from three locations, namely Ibeshe, Amuwo Odofin, and Ojo along Badagry lagoon, Lagos, Nigeria. The samples of the lagoon's surface water and sediments were treated and analyzed for the abundance of microplastics, as well as their shapes, sizes, and types of polymers. The risk index of the polymers in the microplastics was also estimated. Microplastics were found to be more abundant (p ≤ 0.05) in the sediments (283–315 particles/kg) than in the surface water (108–199 particles/L). In both the water and sediments at all the locations, the dominant shapes were fibers (52%–90%), followed by fragments (3%–32%) and films (1%–25%). In order of significance, the microplastic size range of 0-100µm and 100-500µm dominated the surface water, while the size range of 1000-5000µm and 500-1000µm dominated the sediments at all the locations. The dominant polymers in both the water and sediments at all the locations were polyethylene, polypropylene, and polyamide, while the least was polystyrene. In both the water and sediments at all the locations, the dominant risk score among the polymers is III (moderate risk). The results obtained suggest that microplastic pollution poses environmental and health risks to the lagoon, aquatic organisms, and humans. As such, the lagoon required microplastic remediation and control.

Multiwalled carbon nanotubes (MWCNTs) were oxidized using a mixture of H₂SO₄ and HNO₃, and the oxidized MWCNTS were decorated with magnetite (Fe₃O₄). Finally, poly-N-isopropyl acrylamide-co-butyl acrylate (P-NIPAM) was added to obtain P-NIPAM/Fe/MWCNT nanocomposites. The nanosorbents were characterized by various techniques, including X-ray diffraction, transmission electron microscopy, scanning electron microscopy, thermogravimetric analysis, and Brunauer–Emmett–Teller analysis. The P-NIPAM/Fe/MWCNT nanocomposites exhibited increased surface hydrophobicity. Owing to their higher adsorption capacity, their kerosene removal efficiency was 95%; by contrast, the as-prepared, oxidized, and magnetite-decorated MWCNTs had removal efficiencies of 45%, 55%, and 68%, respectively. The P-NIPAM/Fe/MWCNT nanocomposites exhibited a sorbent capacity of 8.1 g/g for kerosene removal from water. The highest kerosene removal efficiency from water was obtained at a process time of 45 min, sorbent dose of 0.005 g, solution temperature of 40 °C, and pH 3.5. The P-NIPAM/Fe/MWCNTs showed excellent stability after four cycles of kerosene removal from water followed by regeneration. The reason may be the increase in the positive charge of the polymer at pH 3.5 and the increased adsorption affinity of the adsorbent toward the kerosene contaminant. The pseudo second-order model was found to be the most suitable model for studying the kinetics of the adsorption reaction.

In recent decades, nanotechnology has rapidly developed. Therefore, there is growing concern about the potential environmental risks of nanoparticles (NPs). Caenorhabditis elegans (C. elegans) has been used as a powerful tool for studying the potential ecotoxicological impacts of nanomaterials from the whole animal level to single cell level, especially in the area of reproduction. In this review, we discuss the reproductive toxicity of common nanomaterials in C. elegans, such as metal-based nanomaterial (silver nanoparticles (NPs), gold NPs, zinc oxide NPs, copper oxide NPs), carbon-based nanomaterial (graphene oxide, multi-walled carbon nanotubes, fullerene nanoparticles), polymeric NPs, silica NPs, quantum dots, and the potential mechanisms involved. This insights into the toxic effects of existing nanomaterials on the human reproductive system. In addition, we summarize how the physicochemical properties (e.g., size, charge, surface modification, shape) of nanomaterials influence their reproductive toxicity. Overall, using C. elegans as a platform to develop rapid detection techniques and prediction methods for nanomaterial reproductive toxicity is expected to reduce the gap between biosafety evaluation of nanomaterials and their application.

Titanium dioxide nanoparticles (TiO₂ NPs) easily combine with other pollutants such as heavy metals because of their excellent physiochemical properties. However, how such an interaction may affect the binding behavior of metals onto biofilms remains largely unclear. This study, examined the effects of TiO₂ NPs on Cd²⁺ accumulation and toxicity for natural periphytic biofilms were examined. The adsorption kinetics showed that adding 0.1 and 1 mg/L TiO₂–NPs increased the Cd²⁺ adsorption of biofilms at equilibrium by 23.5% and 35.8%, respectively. However, adding 10 mg/L TiO₂ NPs increased the Cd²⁺ adsorption of biofilms at equilibrium by only 1.9%. The adsorption isotherms indicate that the presence of TiO₂ NPs considerably increased the Cd²⁺ adsorption capacity of the biofilms; however, this effect became less prominent at high TiO₂ NP concentrations. The optimum pH for Cd²⁺ adsorption increased with increasing Cd²⁺ and TiO₂ NP contents. At low concentrations, the coexistence of Cd²⁺ and TiO₂ NPs may facilitate their respective accumulation by stimulating the secretion of extracellular polymeric substances and enhancing the microbial activity of the biofilm. The presence of TiO₂ NPs increases the surface binding energy between Cd²⁺ and functional groups such as carboxyl groups, enhancing the Cd²⁺ accumulation on the biofilm.

Granular activated carbon (GAC) has been used to remove per- and polyfluoroalkyl substances (PFASs) from industrial or AFFF-impacted waters, but its effectiveness can be low because adsorption of short-chained PFASs is ineffective and its sites are exhausted rapidly by co-contaminants. To increase adsorption of anionic PFASs on GAC by electrostatic attractions, we modified GAC's surface with the cationic polymer poly diallyldimethylammonium chloride (polyDADMAC) and tested its capacity in complex water matrices containing dissolved salts and humic acid. Amending with concentrations of polyDADMAC as low as 0.00025% enhanced GAC's adsorption capacity for PFASs, even in the presence of competing ions. This suggests that electrostatic interactions with polyDADMAC's quaternary ammonium functional groups helped bind organic and inorganic ions as well as the headgroup of short-chain PFASs, allowing more overall PFAS removal by GAC. Evaluating the effect of polymer dose is important because excessive addition can block pores and reduce overall PFAS removal rather than increase it. To decrease the waste associated with this adsorption strategy by making the adsorbent viable for more than one saturation cycle, a regeneration method is proposed which uses low-power ultrasound to enhance the desorption of PFASs from the polyDADMAC-GAC with minimum disruption to the adsorbent's structure. Re-modification with the polymer after sonication resulted in a negligible decrease in the sorbent's capacity over four saturation rounds. These results support consideration of polyDADMAC-modified GAC as an effective regenerable adsorbent for ex-situ concentration step of both short and long-chain PFASs from real waters with high concentrations of competing ions and low PFAS loads.

Systemic studies on the bioremediation of co-contaminated PAHs and heavy metals are lacking, and this paper provides an in-depth review on the topic. The released sources and transport of co-contaminated PAHs and heavy metals, including their co-occurrence through formation of cation-π interactions and their adsorption in soil are examined. Moreover, it is investigated that co-contamination of PAHs and heavy metals can drive a synergistic positive influence on bioremediation through enhanced secretion of extracellular polymeric substances (EPSs), production of biosynthetic genes, organic acid and enzymatic proliferation. However, PAHs molecular structure, PAHs-heavy metals bioavailability and their interactive cytotoxic effects on microorganisms can exert a challenging influence on the bioremediation under co-contaminated conditions. The fluctuations in bioavailability for microorganisms are associated with soil properties, chemical coordinative interactions, and biological activities under the co-contaminated PAHs-heavy metals conditions. The interactive cytotoxicity caused by the emergence of co-contaminants includes microbial cell disruption, denaturation of DNA and protein structure, and deregulation of antioxidant biological molecules. Finally, this paper presents the emerging strategies to overcome the bioavailability problems and recommends the use of biostimulation and bioaugmentation along with the microbial immobilization for enhanced bioremediation of PAHs-heavy metals co-contaminated sites. Better knowledge of the bioremediation potential is imperative to improve the use of these approaches for the sustainable and cost-effective remediation of PAHs and heavy metals co-contamination in the near future.

To better promote environment friendly development of the coal chemical industry, this study investigated effects of methanol, sodium citrate, and chlorella powder (a type of microalgae) as co-metabolic substances on enhanced anaerobic treatment of coal pyrolysis wastewater with anaerobic sludge. The anaerobic sludge was loaded into four 2 L anaerobic reactors for co-metabolism enhanced anaerobic experiments. Anaerobic reactor 1 (R1) as control group did not add a co-metabolic substance; anaerobic reactor 2 (R2) added methanol; anaerobic reactor 3 (R3) added sodium citrate; and anaerobic reactor 4 (R4) added chlorella powder. In the blank control group, the removal ratios of total phenol (TPh), quinoline, and indole were only 12.07%, 42.15%, and 50.47%, respectively, indicating that 50 mg/L quinoline, 50 mg/L indole, and 600 mg/L TPh produced strong toxicity inhibition function on the anaerobic microorganism in reactor. When the concentration of methanol, sodium citrate, and chlorella was 400 μg/L, the reactors with co-metabolic substances had better treatment effect on TPh. Among them, the strengthening effects of sodium citrate (TPh removal ratio: 44.87%) and chlorella (47.85%) were better than that of methanol (38.72%) and the control group (10.62%). Additionally, the reactors with co-metabolic substances had higher degradation ratios on quinoline, indole, and chemical oxygen demand (COD). The data of extracellular polymeric substances showed that with the co-metabolic substances, anaerobic microorganisms produced more humic acids by degrading phenols and nitrogen-containing heterocyclic compounds (NHCs). Compared with the control group, the reactors added with sodium citrate and chlorella had larger average particle size of sludge. Thus, sodium citrate and chlorella could improve sludge sedimentation performance by increasing the sludge particle size. The bacterial community structures of reactors were explored and the results showed that Aminicenantes genera incertae sedis, Levinea, Geobacter, Smithella, Brachymonas, and Longilinea were the main functional bacteria in reactor added with chlorella.

It is difficult to achieve simultaneous and efficient removal of heterocyclic drugs (HCDs) and total nitrogen (TN) in conventional denitrification biofilter (DNBF). Inspired by the effective degradation of refractory organic matter by cobalt-based advanced oxidation process and the need for in-situ upgrading of DNBF, peracetic acid (PAA)/cobalt-loaded ceramsite-based DNBF system was constructed for the first time to treat biochemical tailwater containing HCDs. Results showed that PAA/Co-DNBF had relatively high removal rates for the four HCDs with the order of CBZ > TMP > SDZ > SMX, and the optimal DNBF was H2 with 150 μg L⁻¹of PAA. Overall, TN and HCDs removal increased by 178%–455% and 2.50%–40.99% respectively. When the influent concentration of NO₃⁻-N, COD and each HCDs of 20 mg/L, 60 mg/L and 20 μg/L, below 15 mg/L of effluent TN and the highest average removal rate of SMX (67.77%) could be achieved, under HRT of 4 h in H2. More even distribution of microbial species and low acute toxicity of effluent were also achieved. More even distribution of microbial species and low acute toxicity of effluent were also achieved. In addition, high extracellular polymeric substance (EPS) content and Gordonia after the addition of PAA contributed to the degradation of HCDs. This study supplied a potentially effective strategy for the treatment of biochemical tailwater containing HCDs and provided new insight into the advance of denitrification technology.

The current study for the first time demonstrates the interference of a free-living, N₂-fixing, and nanoparticle (NP) tolerant Azotobacter salinestris strain ASM recovered from metal-polluted soil with tomato plant-metal oxide NPs (ZnO, CuO, Al₂O₃, and TiO₂) interactions in a sandy clay loam soil system with bulk materials as control. Tomato plants were grown till full maturity in soils amended with 20–2000 mg kg⁻¹ of each metal-oxide NP with and without seed biopriming and root-inoculation of A. salinestris. A. salinestris was found metabolically active, producing considerably high amounts of bioactive indole-3-acetic-acid, morphologically unaffected, and with low alteration of cell membrane permeability under 125–1500 μgml⁻¹ of NPs. However, ZnO-NPs slightly alter bacterial membrane permeability. Besides, A. salinestris secreted significantly higher amounts of extracellular polymeric substance (EPS) even under NP exposure, which could entrap the NPs and form metal-EPS complex as revealed and quantified by SEM-EDX. NPs were also found adsorbed on bacterial biomass. EPS stabilized the NPs and provided negative zeta potential to NPs. Following soil application, A. salinestris improved the plant performance and augmented the yield of tomato fruits and lycopene content even in NPs stressed soils. Interestingly, A. salinestris inoculation enhanced photosynthetic pigment formation, flower attributes, plant and fruit biomass, and reduced proline level. Bacterial inoculation also reduced the NP’s uptake and accumulation significantly in vegetative organs and fruits. The organ wise order of NP’s internalization was roots > shoots > fruits. Conclusively, A. salinestris inoculation could be an alternative to increase the production of tomato in metal-oxide NPs contaminated soils.

Estuarine ecosystems near mega-cities are sinks of anthropogenic endocrine disrupting chemicals (EDCs). As the most important primary producer, indigenous microalgae and their secreted extracellular polymeric substances (EPSs) might interact with EDCs and contribute to their fate and risk. Tetraselmis sp. is a representative model of estuarine microalga, for which EDC toxicity and its effects on EPS synthesis have rarely been studied. Through microalgal isolation, algal cell growth tests, EDC removal and the characterization of related EPS profiles, the present work intends to clarify the comparative responses of Tetraselmis sp. to nonylphenol (NP), bisphenol A (BPA) and 17α-ethinylestradiol (EE₂). The results showed that the half inhibitory concentration on cell growth was 0.190–0.313 mg/dm³ for NP, which was one order of magnitude lower than the comparable values for BPA and EE₂ at 2.072–3.254 mg/dm³. Regarding chlorophyll, NP induced its degradation, EE₂ led to its decreased production, and BPA had no obvious effect. Under EDC stress, only the concentrations of colloidal polysaccharides and proteins responded dose-dependently to EE₂. Except for the colloidal fraction in the EE₂ treatment group, the increase in neutral monosaccharides, especially glucose and galactose, was a common response to EDCs. Compared to the recalcitrant BPA, NP underwent abiotic degradation in alga-free water, and EE₂ could be biodegraded in water containing this microalga. The chemical-specific responses of cell growth, chlorophyll and related EPS profiles were driven by the different fates of EDCs, and the underlying mechanism was further discussed. The results obtained in the present work are of critical importance for understanding the fate and effects of different EDCs mediated by microalgae and their related EPSs.