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The Effect of Monsoon on Chemical Composition and Bioaccumulation of Heavy Metals in Scomberomorus commerson, Lacepede 1800, from Oman Sea
2021
Esmaeilzade Ashini, Asma | Sadeghi, Parvin | Tootooni, Mohammad Mansoor
This study was performed to determine the chemical compositions and heavy metals in the muscle of Scomberomorus commerson from the Oman Sea, during the two seasons, pre-monsoon and post-monsoon in 2018. The protein, fat, moisture, and ash contents were determined by AOAC (Association of Official Analytical Chemists) methods. Heavy metal (Zn, Cu, and Pb) analyses were performed by atomic absorption spectrophotometer after acid digestion. There were significant differences between protein, fat, moisture, and ash values in muscle tissue in two seasons (P< 0.05). The highest content of protein (22.53±2.09%) and fat (4.15±1.25%) was recorded in pre-monsoon. The mean concentrations of heavy metals (μg g-1dw) in muscle tissue were 0.08-0.05 for Zn, 0.04-0.02 for Cu, and 0.02-0.01 for Pb in the pre and post-monsoon, respectively. The accumulation of heavy metals in muscle followed the Zn>Cu>Pb. The amounts of Zn, Cu, and Pb were below maximum permissible limits (MPL) recommended by international standards (FAO, FAO/WHO, and MAFF). Results revealed that estimated daily and weekly intakes of Zn, Cu, and Pb were far below the permissible tolerable daily intake (PTWI) recommended by FAO/WHO. Therefore, consumption of S. commerson in the pre and post-monsoon has no risks for human health in the Oman Sea.
Show more [+] Less [-]Profiling of main metabolites in root exudates and mucilage collected from maize submitted to cadmium stress
2019
Lapie, Clémentine | Leglize, Pierre | Paris, Cédric | Sterckeman, Thibault | Buisson, Tatiana | Laboratoire Sols et Environnement (LSE) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Laboratoire d'Ingénierie des Biomolécules (LIBio) ; Université de Lorraine (UL) | Plateau d’Analyse Structurale et Métabolomique (PASM) ; Université de Lorraine (UL)
The aim of this study was to characterize qualitatively and quantitatively the composition of the main rhizodeposits emitted from maize (Zea mays) under Cd stress, in order to discuss their role in Cd availability and tolerance. Maize was grown for 6weeks in sand at four Cd exposure levels (0, 10, 20, and 40M Cd in nutrient solution) and two types of rhizodeposits were collected at the end of cultivation period. Mucilage and other molecules adhering to rhizospheric sand were extracted with a buffer before root exudates were collected by diffusion into water. Total carbon, proteins, amino acids, and sugars were analyzed for both rhizodeposit types and about 40 molecules were identified using GC-MS and LC-MS. Cadmium effect on plant morphology and functioning was slight, but consistent with previous works on Cd toxicity. However, rhizodeposition did tend to be impacted, with a decrease in total carbon, sugars, and amino acids correlating with an increasing Cd content. Such a decrease was not noticeable for proteins in root exudates. These observations were confirmed by the same trends in individual compound contents, although the results were generally not statistically significant. Many of the molecules determined are well-known to modify, whether directly or indirectly, Cd speciation and dynamics in the soil and could play a role in Cd tolerance.
Show more [+] Less [-]Digestive solubilization of Cd in highly-contaminated sediment by marine deposit feeders: The roles of intestinal surfactants in Cd mobilization and Re-Adsorption processes
2020
Wu, Xing | Klerks, Paul L. | Bi, Ran | Liu, Wenhua | Yuan, Zi-Dan | Ma, Xu | Zhang, Guo-Qing | Wang, Shao-Feng | Jia, Yong-Feng
Marine deposit feeders are of ecological significance in transferring sedimentary Cd along aquatic food chains. A key process for this transfer is these organisms’ dietary uptake of Cd via solubilization of Cd present in ingested contaminated sediment. To better understand the bioavailability of sedimentary Cd to deposit feeders, the present study used in vitro extraction experiments to explore the contribution of different digestive agents (proteins, amino acids and surfactants) to the solubilization of Cd from sediment collected in a highly-contaminated Chinese bay. This was done for various commercially-available mimetic digestive agents (the protein BSA, a mixture of amino acids, and the surfactants rhamnolipid and SDS), and for proteins and surfactants collected from the gut juice of a sipunculan worm. The Cd mobilization capacity of BSA was significantly higher than that of the amino acids and the commercial surfactants. In the presence of BSA, > 70% of the released Cd became associated with this protein. In contrast, the digestive proteins from the sipunculan had a lower Cd mobilization capacity than was the case for the other digestive agents and the majority of the released Cd (∼80%) was associated with small molecular weight fractions. The differences in Cd mobilization between the BSA and the digestive proteins were attributed to differences in their sediment-adsorption tendencies and their Cd-complexing capacities. While the digestive surfactants had minor effects on the release of sedimentary Cd, they significantly enhanced Cd mobilization by the digestive proteins when both were present simultaneously. Our results suggest that the characteristics of proteins should be considered when using commercially-available mimetic digestive agents to explore Cd bioavailability in sediments. Furthermore, digestive surfactants seem to have important effects on the solubilization of Cd during gut passage by reducing the adsorption of the digestive proteins to the sediments.
Show more [+] Less [-]Physiological and genotype-specific factors associated with grain quality changes in rice exposed to high ozone
2016
Jing, Liquan | Dombinov, Vitalij | Shen, Shibo | Wu, Yanzhen | Yang, Lianxin | Wang, Yunxia | Frei, Michael
Rising tropospheric ozone concentrations in Asia affect the yield and quality of rice. This study investigated ozone-induced changes in rice grain quality in contrasting rice genotypes, and explored the associated physiological processes during the reproductive growth phase. The ozone sensitive variety Nipponbare and a breeding line (L81) containing two tolerance QTLs in Nipponbare background were exposed to 100 ppb ozone (8 h per day) or control conditions throughout their growth. Ozone affected grain chalkiness and protein concentration and composition. The percentage of chalky grains was significantly increased in Nipponbare but not in L81. Physiological measurements suggested that grain chalkiness was associated with a drop in foliar carbohydrate and nitrogen levels during grain filling, which was less pronounced in the tolerant L81. Grain total protein concentration was significantly increased in the ozone treatment, although the albumin fraction (water soluble protein) decreased. The increase in protein was more pronounced in L81, due to increases in the glutelin fraction in this genotype. Amino acids responded differently to the ozone treatment. Three essential amino acids (leucine, methionine and threonine) showed significant increases, while seven showed significant treatment by genotype interactions, mostly due to more positive responses in L81. The trend of increased grain protein was in contrast to foliar nitrogen levels, which were negatively affected by ozone. A negative correlation between grain protein and foliar nitrogen in ozone stress indicated that higher grain protein cannot be explained by a concentration effect in all tissues due to lower biomass production. Rather, ozone exposure affected the nitrogen distribution, as indicated by altered foliar activity of the enzymes involved in nitrogen metabolism, such as glutamine synthetase and glutamine-2-oxoglutarate aminotransferase. Our results demonstrate differential responses of grain quality to ozone due to the presence of tolerance QTL, and partly explain the underlying physiological processes.
Show more [+] Less [-]Ozone effects on wheat grain quality – A summary
2015
Broberg, Malin C. | Feng, Zhaozhong | Xin, Yue | Pleijel, Håkan
We synthesized the effects of ozone on wheat quality based on 42 experiments performed in Asia, Europe and North America. Data were analysed using meta-analysis and by deriving response functions between observed effects and daytime ozone concentration. There was a strong negative effect on 1000-grain weight and weaker but significant negative effects on starch concentration and volume weight. For protein and several nutritionally important minerals (K, Mg, Ca, P, Zn, Mn, Cu) concentration was significantly increased, but yields were significantly decreased by ozone. For other minerals (Fe, S, Na) effects were not significant or results inconclusive. The concentration and yield of potentially toxic Cd were negatively affected by ozone. Some baking properties (Zeleny value, Hagberg falling number) were positively influenced by ozone. Effects were similar in different exposure systems and for spring and winter wheat. Ozone effects on quality should be considered in future assessments of food security/safety.
Show more [+] Less [-]High time-resolved variations of proteins in PM2.5 during haze pollution periods in Xi'an, China
2022
Yanpeng, Li | Haoyue, Zhang | Aotang, Li | Jiali, Zhang | Shengli, Du
Proteinaceous matter is an important component of PM₂.₅, which can cause adverse health effects and also influence the air quality and climate change. However, there is little attention to high time-resolved variations and potential role of aerosol proteins during haze pollution periods. In this study, PM₂.₅ samples were first collected by a medium flow sampler in autumn and winter in Xi'an, China. Then three high time-resolved monitoring campaigns during haze pollution periods were conducted to determine the evolving characteristics of total protein concentration and explore the interactive relationship between protein and other chemical compositions. The results showed that the average protein concentration in PM₂.₅ in Xi'an (5.46 ± 3.32 μg m⁻³) was higher than those in most cities of China, and varied by seasons and air pollution conditions. In particular, the protein concentration in PM₂.₅ increased with the increase of air quality index (AQI). The continuous variations of aerosol proteins during the haze pollution periods further showed that PM₂.₅, atmospheric humidity and long-distance air mass transport exerted the significant impacts on the protein components in aerosols. Based on the present observation, it is suggested that aerosol proteins might affect the generation of secondary aerosols under haze weather conditions. The present results may provide a new possible insight into the variations and the role of aerosol proteinaceous matter during the formation and development of haze pollution.
Show more [+] Less [-]The complexation with proteins in extracellular polymeric substances alleviates the toxicity of Cd (II) to Chlorella vulgaris
2020
Xie, Qiting | Liu, Na | Lin, Daohui | Qu, Ruohua | Zhou, Qiongzhi | Ge, Fei
The complexation with extracellular polymeric substances (EPS) greatly reduces the toxicity of heavy metals towards organisms in the environment. However, the molecular mechanism of EPS−metal complexation remains unclear owing to the limitation of precise analysis for key fractions and functionalities in EPS that associate with metals. Herein, we explored the EPS−Cd (II) complexation by fluorescence excitation emission matrix coupled with parallel factor (EEM−PARAFAC), two-dimensional Fourier transform infrared correlation spectroscopy (2D-FTIR−COS) and X-ray photoelectron spectroscopy (XPS), attempting to explain the mechanisms of EPS in alleviating Cd (II) toxicity toward a green alga Chlorella vulgaris (C. vulgaris). When the algal EPS were removed, the cell internalizations of Cd (II), growth inhibition rate and chlorophyll autofluorescence increased, but the surface adsorption and esterase activities decreased, indicating that the sorption of Cd (II) by EPS was crucial in alleviating the algal toxicity. Moreover, the complexation with proteins in EPS controlled the sorption of Cd (II) to algal EPS, resulting in the chemical static quenching of the proteins fluorescence by 47.69 ± 2.37%. Additionally, the complexing capability of the main functionalities, COO⁻ and C–OH in proteins with Cd (II) was stronger than that of C–O(H) and C–O–C in polysaccharides or C–OH in the humus-related substances. Oxygen atom in protein carboxyl C–O might be the key site of EPS−Cd (II) complexation, supported by the modified Ryan−Weber complexation model and the obvious shift of oxygen valence-electron signal. These findings provide deep insights into understanding the interaction of EPS with heavy metals in aquatic environment.
Show more [+] Less [-]Transport of biochar colloids in saturated porous media in the presence of humic substances or proteins
2019
Yang, Wen | Bradford, Scott A. | Wang, Yang | Sharma, Prabhakar | Shang, Jianying | Li, Baoguo
Application of biochar in the field has received considerable attention in recent years, but there is still little known about the fate and transport of biochar colloids (BCs) in the subsurface. Natural organic matter (NOM), which mainly consists of humic substance (HS) and proteins, is ubiquitous in the natural environment and its dissolved fraction is active and mobile. In this study, the transport of BCs in saturated porous media has been examined in the presence of two HS (humic and fulvic acids) and two proteins. Bull serum albumin (BSA) and Cytochrome c (Cyt) were selected to present the negatively and positively charged protein, respectively. At low and high salt concentration and different pH conditions, the transport of BCs was strongly promoted by HS. HS significantly increased the mobility of BCs in porous media under both low and high salt conditions due to the enhanced electrostatic repulsion and modification of surface roughness and charge heterogeneity. While BC mobility in porous media was suppressed by both BSA and Cyt in the low salt solution, the presence of BSA largely promoted and Cyt slightly enhanced the transport of BCs in high salt solutions. BSA and Cyt adsorption onto BC surface decreased the negative charge of BC and resulted in a less repulsive interaction in low salt solutions. In high salt solutions, the adsorbed BSA layers disaggregated BCs and reduced the strength of the interaction between BC and the sand. Adsorbed Cyt on BCs caused more attractive patches between BC and sand surface, and greater retention than BSA.
Show more [+] Less [-]The roles of protein and lipid in the accumulation and distribution of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in plants grown in biosolids-amended soils
2016
Wen, Bei | Wu, Yali | Zhang, Hongna | Liu, Yu | Hu, Xiaoyu | Huang, Honglin | Zhang, Shuzhen
The roles of protein and lipid in the accumulation and distribution of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in seven species of plants from biosolids-amended soils were investigated. The PFOS and PFOA root concentration factors (Croot/Csoil) ranged from 1.37 to 4.68 and 1.69 to 10.3 (ng/groot)/(ng/gsoil), respectively, while the translocation factors (Cshoot/Croot) ranged from 0.055 to 0.16 and 0.093 to 1.8 (ng/gshoot)/(ng/groot), respectively. The PFOS and PFOA accumulations in roots correlated positively with root protein contents (P < 0.05), while negatively with root lipid contents (P < 0.05). These suggested the promotion effects of protein and inhibition effects of lipid on root uptake. The translocation factors correlated positively with the ratios between protein contents in shoots to those in roots (P < 0.05), showing the importance of protein on PFOS and PFOA translocation. This study is the first to reveal the different roles of protein and lipid in the accumulation and distribution of PFOS and PFOA in plants.
Show more [+] Less [-]CO2 dose–response functions for wheat grain, protein and mineral yield based on FACE and open-top chamber experiments
2015
Pleijel, Håkan | Högy, Petra
Data from three Swedish open-top chamber and four German FACE experiments were combined to derive response functions for elevated CO2 (eCO2) effects on Cd, Zn, Mn, protein, grain yield, grain mass and grain number of wheat. Grain yield and grain number were increased by ∼6% and ∼7%, respectively, per 100 ppm CO2; the former effect was linked to plant nitrogen status. Grain mass was not influenced by eCO2, whereas Cd concentration was reduced. Unlike Zn, Mn and protein, effects on Cd yield were not related to effects on grain yield. Yields of Mn, Zn and (weakly) protein were positively affected by eCO2. For protein, grain yield, grain mass and grain number, the results were consistent among the FACE and OTC experiments. A key conclusion was that yields of essential nutrients were enhanced (Mn > Zn > protein), although less than grain yield, which would not be expected from a simple dilution model.
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