International audience | Copper (Cu) contamination of soils may alter the functioning and sustainability of vineyard ecosystems. Cultivating Cu-extracting plants in vineyard inter-rows, or phytoextraction, is one possible way currently under consideration in agroecology to reduce Cu contamination of vineyard topsoils. This option is rarely used, mainly because Cu phytoextraction yields are too low to significantly reduce contamination due to the relatively "low" phytoavailability of Cu in the soil (compared to other trace metals) and its preferential accumulation in the roots of most extracting plants. This article describes the main practices and associated constraints that could theoretically be used to maximize Cu phytoextraction at field scale, including the use of Cu-accumulating plants grown (i) with acidifying plants (e.g., leguminous plants), and/or (ii) in the presence of acidifying fertilizers (ammonium, elemental sulfur), or (iii) with soluble "biochelators" added to the soil such as natural humic substances or metabolites produced by rhizospheric bacteria such as siderophores, in the inter-rows. This discussion article also provides an overview of the possible ways to exploit Cu-enriched biomass, notably through ecocatalysis or biofortification of animal feed.

Copper (Cu) contamination of soils may alter the functioning and sustainability of vineyard ecosystems. Cultivating Cu-extracting plants in vineyard inter-rows, or phytoextraction, is one possible way currently under consideration in agroecology to reduce Cu contamination of vineyard topsoils. This option is rarely used, mainly because Cu phytoextraction yields are too low to significantly reduce contamination due to the relatively “low” phytoavailability of Cu in the soil (compared to other trace metals) and its preferential accumulation in the roots of most extracting plants. This article describes the main practices and associated constraints that could theoretically be used to maximize Cu phytoextraction at field scale, including the use of Cu-accumulating plants grown (i) with acidifying plants (e.g., leguminous plants), and/or (ii) in the presence of acidifying fertilizers (ammonium, elemental sulfur), or (iii) with soluble “biochelators” added to the soil such as natural humic substances or metabolites produced by rhizospheric bacteria such as siderophores, in the inter-rows. This discussion article also provides an overview of the possible ways to exploit Cu-enriched biomass, notably through ecocatalysis or biofortification of animal feed.

International audience | Plants adapt to metal stress by modifying their metabolism including the production of secondary metabolites in plant tissues. Such changes may impact the diversity and functions of plant associated microbial communities. Our study aimed to evaluate the influence of metals on the secondary metabolism of plants and the indirect impact on rhizosphere bacterial communities. We then compared the secondary metabolites of the hyperaccumulator Pteris vittata L. collected from a contaminated mining site to a non-contaminated site in Vietnam and identified the discriminant metabolites. Our data showed a significant increase in chlorogenic acid derivatives and A-type procyanidin in plant roots at the contaminated site. We hypothesized that the intensive production of these compounds could be part of the antioxidant defense mechanism in response to metals. In parallel, the structure and diversity of bulk soil and rhizosphere communities was studied using high-throughput sequencing. The results showed strong differences in bacterial composition, characterized by the dominance of Proteobacteria and Nitrospira in the contaminated bulk soil, and the enrichment of some potential human pathogens, i.e., Acinetobacter, Mycobacterium, and Cupriavidus in P. vittata's rhizosphere at the mining site. Overall, metal pollution modified the production of P. vittata secondary metabolites and altered the diversity and structure of bacterial communities. Further investigations are needed to understand whether the plant recruits specific bacteria to adapt to metal stress.

Plants adapt to metal stress by modifying their metabolism including the production of secondary metabolites in plant tissues. Such changes may impact the diversity and functions of plant associated microbial communities. Our study aimed to evaluate the influence of metals on the secondary metabolism of plants and the indirect impact on rhizosphere bacterial communities. We then compared the secondary metabolites of the hyperaccumulator Pteris vittata L. collected from a contaminated mining site to a non-contaminated site in Vietnam and identified the discriminant metabolites. Our data showed a significant increase in chlorogenic acid derivatives and A-type procyanidin in plant roots at the contaminated site. We hypothesized that the intensive production of these compounds could be part of the antioxidant defense mechanism in response to metals. In parallel, the structure and diversity of bulk soil and rhizosphere communities was studied using high-throughput sequencing. The results showed strong differences in bacterial composition, characterized by the dominance of Proteobacteria and Nitrospira in the contaminated bulk soil, and the enrichment of some potential human pathogens, i.e., Acinetobacter, Mycobacterium, and Cupriavidus in P. vittata’s rhizosphere at the mining site. Overall, metal pollution modified the production of P. vittata secondary metabolites and altered the diversity and structure of bacterial communities. Further investigations are needed to understand whether the plant recruits specific bacteria to adapt to metal stress.