Multiple sources of microplastics (MPs) in farmland could result in the changing of microbial community and the plant growth. Most studies of MPs in agricultural system have focused on the effects of single types of MPs on growth of plants, while neglect interactions between multiple types of MPs. In this study a pot-experiment was conducted to investigate the effects of multiple types of MPs, including polystyrene beads: M1, 5 μm, M2, 70 nm and degradable mulching film (DMF) fragments on growth of wheat seedlings and associated rhizosphere microbial community. CKD (adding DMF) significantly reduced plant height and base diameter of wheat seedlings. DMF in combination with M2, significantly increased plant height and aboveground biomass, but decreased the base diameter. Actinobacteria was the dominant taxa in the rhizosphere bacterial community in various treatments. PCoA analysis showed that the bacterial composition in M2HD (100 mg kg⁻¹ M² with DMF) was significantly different from that of CKD and M2LD (10 mg kg⁻¹ M² with DMF). At the level of genera, the dominant fungi in CKD and M2LD were in the genus Fusarium, which is the cause of wheat fusarium blight and Alternaria, which results in decreased base diameter. In CK (control group) and M2HD, Blastobotrys exhibited the greatest abundance, which assisted wheat seedlings in resisting Verticillium disease. Cluster and PCoA analysis showed the fungal composition in CKD was significantly different from CK, M2LD and M2HD. These findings suggest MPs potentially have selective effects on pathogens that affect growth of plants and potentially safety of the food.

Arsenic (As) and cadmium (Cd), two major carcinogenic heavy metals, enters into human food chain by the consumption of rice or rice-based food products. Both As and Cd disturb plant-nutrient homeostasis and hence, reduces plant growth and crop productivity. In the present study, As/Cd modulated responses were studied in non-basmati (IR-64) and basmati (PB-1) rice varieties, at physiological, biochemical and transcriptional levels. At the seedling stage, PB-1 was found more sensitive than IR-64, in terms of root biomass; however, their shoot phenotype was comparable under As and Cd stress conditions. The ionomic data revealed significant nutrient deficiencies in As/Cd treated-roots. The principal component analysis identified NH₄⁺ as As-associated key macronutrient; while, NH₄⁺/NO₃⁻ and K⁺ was majorly associated with Cd mediated response, in both IR-64 and PB-1. Using a panel of 21 transporter gene expression, the extent of nutritional deficiency was ranked in the order of PB-1(As)<IR-64(As)<PB-1(Cd)<IR-64(Cd). A feed-forward model is proposed to explain nutrient deficiency induced de-regulation of gene expression, as observed under Cd-treated IR-64 plants, which was also validated at the level of sulphur metabolism related enzymes. Using urea supplementation, as nitrogen-fertilizer, significant mitigation was observed under As stress, as indicated by 1.018- and 0.794-fold increase in shoot biomass in IR-64 and PB-1, respectively compared to that of control. However, no significant amelioration was observed in response to supplementation of urea under Cd or potassium under As/Cd stress conditions. Thus, the study pinpointed the relative significance of various macronutrients in regulating As- and Cd-tolerance and will help in designing suitable strategies for mitigating As and/or Cd stress conditions.

High cadmium (Cd) concentration in common wheat (Triticum aestivum L.) grains poses potential health risks. Several management strategies have been used to reduce grain Cd concentration. However, limited information is available on the use of ammonium-nitrogen (NH₄⁺-N) as a strategy to manage Cd concentration in wheat grains. In this study, NH₄⁺-N addition at the seedling stage unchanged the grain Cd concentration in the high-Cd accumulator, Zhoumai 18 (ZM18), but dramatically increased that in the low-Cd accumulator, Yunmai 51 (YM51). Further analysis revealed that the effects of NH₄⁺-N addition on whole-plant Cd absorption, root-to-shoot Cd translocation, and shoot-to-grain Cd remobilization were different between the two wheat cultivars. In ZM18, NH₄⁺-N addition did not change whole-plant Cd absorption, but inhibited root-to-shoot Cd translocation and Cd remobilization from lower internodes, lower leaves, node 1, and internode 1 to grains via the down-regulation of yellow stripe-like transporters (YSL), zinc transporters (ZIP5, ZIP7, and ZIP10), and heavy-metal transporting ATPases (HMA2). This inhibition decreased the grain Cd content by 29.62%, which was consistent with the decrease of the grain dry weight by 23.26%, leading to unchanged grain Cd concentration in ZM18. However, in YM51, NH₄⁺-N addition promoted continuous Cd absorption during grain filling, root-to-shoot Cd translocation and whole-plant Cd absorption. The absorbed Cd was directly transported to internode 1 via the xylem and then re-transported to grains via the phloem by up-regulated YSL, ZIP5, and copper transporters (COPT4). This promotion increased the grain Cd content by 245.35%, which was higher than the increased grain dry weight by 132.89%, leading to increased grain Cd concentration in YM51. Our findings concluded that the addition of NH₄⁺-N fertilizer at the seedling stage is not suitable for reducing grain Cd concentration in common wheat cultivars.

Due to a close contact with water column, submerged macrophytes are easily disturbed by environment change in freshwater ecosystems, especially at the seedling stage. In recent decades, freshwater ecosystems have been subject to severe cadmium (Cd) pollution, which can cause toxic effects on the growth of submerged macrophytes. Moreover, the temperature rise resulting from climate warming and water level decline may further aggravate such effect, especially in shallow lakes. Here, we investigated the independent and interaction effects of Cd exposure levels (0, 0.5, 1, and 2.5 mg L⁻¹) and temperature (15, 25, and 30 °C) on morphological and physiological traits of Myriophyllum aquaticum (Vell.) Verd. Seedlings generated from propagules and seeds. The temperature rise and Cd exposure generally resulted in a significant increase of Cd concentrations and antioxidant enzyme activities in leaves, as well as a decrease of chlorophyll a and b concentrations. The number and length of leaves generated from propagules always show a downward trend with the increase of Cd exposure, regardless of the temperature. Moreover, the lowest leaf number and length always occurred at high temperature (i.e. 30 °C) when the Cd exposure level increased to 1 and 2.5 mg L⁻¹. For the seedlings generated from seeds, the temperature rise caused an increase of leaf emergence rate under low Cd exposure levels, but resulted in a significant decrease with the Cd exposure level. This study indicates the negative effects of Cd exposure and temperature rise on submerged macrophytes at the seedling stage, and highlights that temperature rise would enhance Cd toxicity.

Pharmacologically active compounds found in reclaimed wastewater irrigation or animal manure fertilizers pose potential risks for agriculture. The mechanism underlying the effects of ketoprofen on rice (Oryza sativa L.) seedlings was investigated. The results showed that low concentrations (0.5 mg L⁻¹) of ketoprofen slightly stimulate growth of rice seedlings, while high concentrations can significantly inhibit growth by reducing biomass and causing damage to roots. Ketoprofen affects photosynthetic pigment content (Chla, Chlb, and carotenoids) and chlorophyll synthesis gene (HEMA, HEMG, CHLD, CHLG, CHLM, and CAO) expression. Fluorescence parameters such as minimum fluorescence (F₀), maximum fluorescence (Fₘ), variable fluorescence (Fᵥ), potential photosynthetic capacity (Fᵥ/F₀), maximum quantum efficiency of PSII photochemistry (Fᵥ/Fₘ), electron transfer rate (ETR), and Y(II), Y(NPQ), Y(NO) values were affected, showing photosynthetic electron transfer was blocked. Active oxygen radical (O₂•−and H₂O₂), malondialdehyde and proline content increased. Superoxide dismutase, catalase and ascorbate peroxidase activities, glutathione content and antioxidant-related gene (FSD1, MSD1, CSD1, CSD2, CAT1, CAT2, CAT3, APX1, APX2) expression were induced. Higher integrated biomarker response values of eight oxidative stress response indexes were obtained at higher ketoprofen concentrations. Ultrastructure observation showed that ketoprofen causes cell structure damage, chloroplast swelling, increase in starch granules, and reduction in organelles. This study provides some suggested toxicological mechanisms and biological response indicators in rice due to stress from pharmacologically active compounds.

Bitumen recovery from oil sands in northeastern Alberta, Canada produces large volumes of tailings, which are deposited in mining areas that must be reclaimed upon mine closure. A new technology of non-segregated tailings (NST) developed by Canadian Natural Resources Limited (CNRL) was designed to accelerate the process of oil sands fine tailings consolidation. However, effects of these novel tailings on plants used for the reclamation of oil sands mining areas remain to be determined. In the present study, we investigated the effects of NST on seedlings of three species of plants commonly planted in oil sands reclamation sites including paper birch (Betula papyrifera), white spruce (Picea glauca) and green alder (Alnus viridis). In the controlled-environment study, we grew seedlings directly in NST and in the two types of reclamation soils with and without added NST and we measured seedling growth, gas exchange parameters, as well as tissue concentrations of selected elements and foliar chlorophyll. White spruce seedlings suffered from severe mortality when grown directly in NST and their needles contained high concentrations of Na. The growth and physiological processes were also inhibited by NST in green alder and paper birch. However, the addition of top soil and peat mineral soil mix to NST significantly improved the growth of plants, possibly due to a more balanced nutrient uptake. It appears that NST may offer some advantages in terms of site revegetation compared with the traditional oil sands tailings that were used in the past. The results also suggest that, white spruce may be less suitable for planting at reclamation sites containing NST compared with the two studied deciduous tree species.