To investigate toxic effects of microplastic on marine microalgae Skeletonema costatum, both algal growth inhibition test and non-contact shading test were carried out, and algal photosynthesis parameters were also determined. The SEM images were used to observe interactions between microplastic and algae. It was found that microplastic (mPVC, average diameter 1 μm) had obvious inhibition on growth of microalgae and the maximum growth inhibition ratio (IR) reached up to 39.7% after 96 h exposure. However, plastic debris (bPVC, average diameter 1 mm) had no effects on growth of microalgae. High concentration (50 mg/L) mPVC also had negative effects on algal photosynthesis since both chlorophyll content and photosynthetic efficiency (ΦPSⅡ) decreased under mPVC treatments. Shading effect was not one reason for toxicity of microplastic on algae in this study. Compared with non-contact shading effect, interactions between microplastic and microalage such as adsorption and aggregation were more reasonable explanations for toxic effects of microplastic on marine microalgae. The SEM images provided a more direct and reasonable method to observe the behaviors of microplastic.

Though the main toxic mechanisms of graphene oxide (GO) to algae have been accepted as the shading effect, oxidative stress and mechanical damage, the effect of algal characteristics on these three mechanisms of GO toxicity have seldom been taken into consideration. In this study, we investigated GO toxicity to green algae (Chlorella vulgaris, Scenedesmus obliquus, Chlamydomonas reinhardtii), cyanobacteria (Microcystis aeruginosa) and diatoms (Cyclotella sp.). The aim was to assess how the physiological characteristics of algae affect the toxicity of GO. Results showed that 10 mg/L of GO significantly inhibited the growth of all tested algal types, while S. obliquus and C. reinhardtii were found to be the most susceptible and tolerant species, respectively. Then, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to observe the physiological characteristics of the assessed algae. The presence of locomotive organelles, along with smaller and more spherical cells, was more likely to alleviate the shading effect. Variations in cell wall composition led to different extents of mechanical damage as shown by Cyclotella sp. silica frustules and S. obliquus autosporine division being prone to damage. Meanwhile, growth inhibition and cell division were significantly correlated with the oxidative stress and membrane permeability, suggesting the latter two indicators can effectively signal GO toxicity to algae. The findings of this study provide novel insights into the toxicity of graphene materials in aquatic environments.

The flux of gaseous elemental mercury (Hg0) from the forest floor of the Adirondack Mountains in New York (USA) was measured numerous times throughout 2005 and 2006 using a polycarbonate dynamic flux chamber (DFC). The Hg flux ranged between -2.5 and 27.2 ng m-2 h-1 and was positively correlated with temperature and solar radiation. The measured Hg emission flux was highest in spring, and summer, and lowest in winter. During leaf-off periods, the Hg emission flux was highly dependent on solar radiation and less dependent on temperature. During leaf-on periods, the Hg emission flux was fairly constant because the forest canopy was shading the forest floor. Two empirical models were developed to estimate yearly Hg0 emissions, one for the leaf-off period and one for the leaf-on period. Using the U.S. EPA's CASTNET meteorological data, the cumulative estimated emission flux was approx. 7.0 μg Hg0 m-2 year-1.

Existing mitigations to address deterioration in water clarity associated with human activities are based on responses from single seagrass species but may not be appropriate for diverse seagrass assemblages common to tropical waters. We present findings from a light experiment designed to determine the effects of magnitude and duration of low light on a mixed tropical seagrass assemblage. Mixed assemblages of three commonly co-occurring Indo-West Pacific seagrasses, Cymodocea serrulata, Halodule uninervis and Halophila ovalis were grown in climate-controlled tanks, where replicate pots were subjected to a gradient in light availability (0.9–21.6 mols PAR m−2 day−1) for 12 weeks. Increased shading resulted in declines in growth and changes in cellular and photosynthesis responses for all species, although time-scale and magnitude of response were species-specific. Applying management criteria (e.g. thresholds) relevant to one species may under- or over-estimate potential for impact on other species and the meadow as a whole.

The impact of pond aquaculture effluents on the distribution and performance of seagrasses was examined in NE Hainan, tropical China. Samples were taken along transects in three back-reef areas with different extent of aquaculture production in their hinterland. High δ15N in seagrass leaves and epiphytes (6–9‰) similar to values in pond effluents documented aquaculture as dominant nitrogen source in the back-reefs with decreasing impact with distance from shore. Seagrass species abundance, shoot density and biomass were lower and concentrations of nutrients, chlorophyll and suspended matter were higher at nearshore sites with high and moderate pond abundance than at the control site. High epiphyte loads and low δ34S in seagrass leaves suggest temporal shading and sulphide poisoning of the nearshore seagrasses. Observed gradients in environmental parameters and seagrass performance indicate that the distance from the pond outlets and size of the adjacent pond agglomeration are major determinants of seagrass degradation.

A review of published literature on the sensitivity of corals to turbidity and sedimentation is presented, with an emphasis on the effects of dredging. The risks and severity of impact from dredging (and other sediment disturbances) on corals are primarily related to the intensity, duration and frequency of exposure to increased turbidity and sedimentation. The sensitivity of a coral reef to dredging impacts and its ability to recover depend on the antecedent ecological conditions of the reef, its resilience and the ambient conditions normally experienced. Effects of sediment stress have so far been investigated in 89 coral species (∼10% of all known reef-building corals). Results of these investigations have provided a generic understanding of tolerance levels, response mechanisms, adaptations and threshold levels of corals to the effects of natural and anthropogenic sediment disturbances. Coral polyps undergo stress from high suspended-sediment concentrations and the subsequent effects on light attenuation which affect their algal symbionts. Minimum light requirements of corals range from <1% to as much as 60% of surface irradiance. Reported tolerance limits of coral reef systems for chronic suspended-sediment concentrations range from <10mgL⁻¹ in pristine offshore reef areas to >100mgL⁻¹ in marginal nearshore reefs. Some individual coral species can tolerate short-term exposure (days) to suspended-sediment concentrations as high as 1000mgL⁻¹ while others show mortality after exposure (weeks) to concentrations as low as 30mgL⁻¹. The duration that corals can survive high turbidities ranges from several days (sensitive species) to at least 5–6weeks (tolerant species). Increased sedimentation can cause smothering and burial of coral polyps, shading, tissue necrosis and population explosions of bacteria in coral mucus. Fine sediments tend to have greater effects on corals than coarse sediments. Turbidity and sedimentation also reduce the recruitment, survival and settlement of coral larvae. Maximum sedimentation rates that can be tolerated by different corals range from <10mgcm⁻²d⁻¹ to >400mgcm⁻²d⁻¹. The durations that corals can survive high sedimentation rates range from <24h for sensitive species to a few weeks (>4weeks of high sedimentation or >14days complete burial) for very tolerant species. Hypotheses to explain substantial differences in sensitivity between different coral species include the growth form of coral colonies and the size of the coral polyp or calyx. The validity of these hypotheses was tested on the basis of 77 published studies on the effects of turbidity and sedimentation on 89 coral species. The results of this analysis reveal a significant relationship of coral sensitivity to turbidity and sedimentation with growth form, but not with calyx size. Some of the variation in sensitivities reported in the literature may have been caused by differences in the type and particle size of sediments applied in experiments. The ability of many corals (in varying degrees) to actively reject sediment through polyp inflation, mucus production, ciliary and tentacular action (at considerable energetic cost), as well as intraspecific morphological variation and the mobility of free-living mushroom corals, further contribute to the observed differences. Given the wide range of sensitivity levels among coral species and in baseline water quality conditions among reefs, meaningful criteria to limit the extent and turbidity of dredging plumes and their effects on corals will always require site-specific evaluations, taking into account the species assemblage present at the site and the natural variability of local background turbidity and sedimentation.

The accumulation of debris is an insidious problem throughout the world’s oceans. Here we document 234.24 items of macro-debris/km² in the shallow populated parts of Majuro lagoon (Republic of the Marshall Islands) which is the second highest standing stock of macro-debris recorded to date in any benthic marine habitat in the world. The majority of macro-debris was from household sources (78.7%) with the peak abundance recorded in areas of medium affluence. Marine debris causes suffocation, shading, tissue abrasion and mortality of corals and we show a significant negative correlation exists between the level of hard coral cover and coverage of marine debris. Given long decomposition times, even if the input of rubbish to Majuro lagoon is stopped immediately, the standing stock of debris will persist for centuries. Multiple new initiatives are needed to curtail the direct and indirect dumping of waste in Majuro lagoon.

In situ studies of plastic deterioration can help us understand the longevity of macroplastic as well as the generation of microplastics in the environment. Photo-oxidation contributing to the generation of microplastics in the marine environment was explored using four types of plastic (polyethene, polystyrene, poly(ethylene terephthalate) and Biothene® exposed in light and in shade, in both air and sea water. Metrics for deterioration were tensile extensibility and oxidation rate. Measurements were conducted at intervals between 7 and 600 days' exposure. Deterioration was faster in air than in sea water and was further accelerated in direct light compared to shade. Extensibility and oxidation were significantly inversely correlated in samples exposed in air. Samples in sea water lost extensibility at a slower rate. Polystyrene, which enters the waste stream rapidly due to its wide application in packaging, deteriorated fastest and is, therefore, likely to form microplastics more rapidly than other materials, especially when exposed to high levels of irradiation, for example when stranded on the shore.

Though seagrass meadows are among the most productive habitats in the world, contributing substantially to long-term carbon storage, studies of the effects of critical disturbances on the fate of carbon sequestered in the sediment and biomass of these meadows are scarce. In a manipulative in situ experiment, we studied the effects of successive loss of seagrass biomass as a result of shading and simulated grazing at two intensity levels on sulphide (H2S) content and methane (CH4) emission in a tropical seagrass meadow in Zanzibar (Tanzania). In all disturbed treatments, we found a several-fold increase in both the sulphide concentration of the sediment pore-water and the methane emissions from the sediment surface (except for CH4 emissions in the low-shading treatment). This could be due to the ongoing degradation of belowground biomass shed by the seagrass plants, supporting the production of both sulphate-reducing bacteria and methanogens, possibly exacerbated by the loss of downwards oxygen transport via seagrass plants. The worldwide rapid loss of seagrass areas due to anthropogenic activities may therefore have significant effects on carbon sink-source relationships within coastal seas.

Seagrass meadows in many parts of the globe are threatened by a range of processes including port development, dredging and land clearing in coastal catchments, which can reduce water clarity and increase sedimentation pressure. As rates of seagrass loss increase, there is an urgent need to understand the potential impacts of development on these critical species. This research compares the effects of shading and burial by fine sand on two seagrass species Zostera muelleri and Halophila ovalis in Port Curtis Bay, an industrial harbour located on the continental margin adjacent to the Great Barrier Reef Heritage Area, Australia. The research finds that shading in combination with burial causes a significant decline in growth rates in both species, but that burial ≥10mm reduces growth rates to a greater extent than shading. The paper concludes by discussing the implications of these findings for port management and impact assessment.