Arsenic (As) contamination and bioaccumulation are a serious threat to agricultural plants. To address this issue, we checked the efficacy of As tolerant plant growth promoting bacteria (PGPB), zinc oxide nanoparticles (ZnO NPs) and oxalic acid (OA) in Luffa acutangula grown on As rich soil. The selected most As tolerant PGPB i.e Providencia vermicola exhibited plant growth promoting features i.e solubilzation of phosphate, potassium and siderophores production. Innovatively, we observed the synergistic effects of P. vermicola, ZnO NPs (10 ppm) and OA (100 ppm) in L. acutangula grown on As enriched soil (150 ppm). Our treatments both as alone and in combination alleviated As toxicity exhibited by better plant growth and metabolism. Results revealed significantly enhanced photosynthetic pigments, proline, relative water content, total sugars, proteins and indole acetic acid along with As amelioration in L. acutangula. Furthermore, upregulated plant resistance was manifested with marked reduction in the lipid peroxidation and electrolyte leakage and pronounced antagonism of As and zinc content in leaves under toxic conditions. These treatments also improved level of nutrients, abscisic acid and antioxidants to mitigate As toxicity. This marked improvement in plants’ defense mechanism of treated plants under As stress is confirmed by less damaged leaves cell structures observed through the scanning electron micrographs. We also found substantial decrease in the As bioaccumulation in the L. acutangula shoots and roots by 40 and 58% respectively under the co-application of P. vermicola, ZnO NPs and OA in comparison with control. Moreover, the better activity of soil phosphatase and invertase was assessed under the effect of our application. These results cast a new light on the application of P. vermicola, ZnO NPs and OA in both separate and combined form as a feasible and ecofriendly tool to alleviate As stress in L. acutangula.

In this study, Bacillus sp. strain AVPP64 was isolated from diuron-contaminated soil. It showed 4-nitroaniline (4-NA) degradation, pesticide tolerance, and self-nutrient integration via nitrogen (N)-fixation and phosphate (P)-solubilization. The rate constant (k) and half-life period (t₁/₂) of 4-NA degradation in the aqueous medium inoculated with strain AVPP64 were observed to be 0.445 d⁻¹ and 1.55 d, respectively. Nevertheless, in the presence of chlorpyrifos, profenofos, atrazine and diuron pesticides, strain AVPP64 degraded 4-NA with t₁/₂ values of 2.55 d, 2.26 d, 2.31 d and 3.54 d, respectively. The strain AVPP64 fixed 140 μg mL⁻¹ of N and solubilized 103 μg mL⁻¹ of P during the presence of 4-NA. In addition, strain AVPP64 produced significant amounts of plant growth-promoting metabolites like indole 3-acetic acid, siderophores, exo-polysaccharides and ammonia. In the presence of 4-NA and various pesticides, strain AVPP64 greatly increased the growth and biomass of Vigna radiata and Crotalaria juncea plants. These results revealed that Bacillus sp. strain AVPP64 can be used as an inoculum for bioremediation of 4-NA contaminated soil and sustainable crop production even when pesticides are present.

In this study, two proficient Cadmium (Cd) resistant and plant growth-promoting actinobacterial strains were isolated from metal-polluted soils and identified as Streptomyces sp. strain RA04 and Nocardiopsis sp. strain RA07. Multiple abiotic stress tolerances were found in these two actinobacterial strains, including Cd stress (CdS), drought stress (DS) and high-temperature stress (HTS). Both actinobacterial strains exhibited multifarious plant growth-promoting (PGP) traits such as phosphate solubilization, and production of indole-3-acetic acid, siderophores and 1-aminocyclopropane-1-carboxylate deaminase under CdS, DS and HTS conditions. The inoculation of strains RA04 and RA07 significantly increased Sorghum bicolor growth and photosynthetic pigments under CdS, DS, HTS, CdS + DS and CdS + HTS conditions as compared to their respective uninoculated plants. The actinobacterial inoculants reduced malondialdehyde concentration and enhanced antioxidant enzymes in plants cultivated under various abiotic stress conditions, indicating that actinobacterial inoculants reduced oxidative damage. Furthermore, strains RA04 and RA07 enhanced the accumulation of Cd in plant tissues and the translocation of Cd from root to shoot under CdS, CdS + DS and CdS + HTS treatments as compared to their respective uninoculated plants. These findings suggest that RA04 and RA07 strains could be effective bio-inoculants to accelerate phytoremediation of Cd polluted soil even in DS and HTS conditions.

This paper analyzes a unique, aquatic phytobial biocenosis that has been forming naturally for over 20 years and operating as a filter for Cr(VI)-polluted groundwater. Our study presents a thorough taxonomic analysis of the biocenosis, including filamentous algae, vascular plants, and microbiome, together with the analysis of Cr accumulation levels, bioconcentration factors and other environmentally-significant parameters: siderophore production by bacteria, biomass growth of the plants or winter hardiness. Among 67 species identified in the investigated reservoir, 13 species were indicated as particularly useful in the bioremediation of Cr(VI)-polluted water and sediment. Moreover, three species of filamentous algae, Tribonema sp., and three easily culturable bacterial species were for the first time shown as resistant to Cr concentration up to 123 mg/dm³, i.e. 6150 times over the permissible level. The work presents a modern holistic phytobial consortium indispensable for the remediation of Cr(VI)-contaminated aquatic environment in temperate zones worldwide.

The impact of siderophore produced by arsenic-resistant bacterium Pseudomonas PG12 on FeAsO4 dissolution and plant growth were examined. Arsenic-hyperaccumulator Pteris vittata was grown for 7 d in 0.2-strength Fe-free Hoagland solution containing FeAsO4 mineral and PG12-siderophore or fungal-siderophore desferrioxamine B (DFOB). Standard siderophore assays indicated that PG12-siderophore was catecholate-type. PG12-siderophore was more effective in promoting FeAsO4 dissolution, and Fe and As plant uptake than DFOB. Media soluble Fe and As in PG12 treatment were 34.6 and 3.07 μM, 1.6- and 1.4-fold of that in DFOB. Plant Fe content increased from 2.93 to 6.24 g kg−1 in the roots and As content increased from 14.3 to 78.5 mg kg−1 in the fronds. Besides, P. vittata in PG12 treatment showed 2.6-times greater biomass than DFOB. While P. vittata fronds in PG12 treatment were dominated by AsIII, those in DFOB treatment were dominated by AsV (61–77%). This study showed that siderophore-producing arsenic-resistant rhizobacteria may have potential in enhancing phytoremediation of arsenic-contaminated soils.

This work aims to explore the role of cadmium-resistant actinomycetes on promoting plant growth and cadmium uptake in Chlorophytum comosum (Thunb.) Jacques, a spider plant. Actinomycetes isolated from the plant roots in peat swamp forests were screened for their cadmium resistance and the production of indole-3-acetic acid (IAA) and siderophores. The results found that K5PN1 and 11-10SHTh produced high levels of IAA and siderophores, respectively. K5PN1 and 11-10SHTh were identified to be Streptomyces rapamycinicus and Streptomyces cyaneus, respectively. Both strains were able to remove cadmium from aqueous solution and survive under cadmium stress in contaminated soil. The results of pot experiments found that the selected Streptomyces inoculation increased the root and shoot biomass and cadmium accumulation in the root and shoot of C. comosum planted in a cadmium-contaminated soil. The highest cadmium accumulation and translocation ability of cadmium from the root to shoot was found in C. comosum with S. rapamycinicus inoculation. In addition, plant with S. cyaneus inoculation had the highest phytoextraction coefficient and bioaccumulation factor. Our findings concluded that S. rapamycinicus and S. cyaneus stimulated the growth and cadmium uptake in C. comosum, suggesting a combined approach using the selected Streptomyces and C. comosum for phytoremediation of cadmium-polluted soil.

Phytostabilisation can benefit from phytostimulatory rhizobacteria. Forty-three bacterial strains were isolated from the roots of the metallicolous legume Anthyllis vulneraria ssp. carpatica grown in a highly contaminated mine tailing (total Cd, Pb and Zn were up to 1200; 34,000; and 170,000 mg kg⁻¹, respectively). We aimed at evaluating their phytostimulatory effects on the development of leguminous metallophytes. Strains were screened for fluorescent siderophores and auxin synthesis, inorganic P solubilisation and 1-amino-cyclopropane-1-carboxylate deaminase (ACCd) activity to define a subset of 11 strains that were inoculated on the leguminous metallophytes A. vulneraria and Lotus corniculatus grown in diluted mine spoil (Zn 34,653; Pb 6842; and Cd 242, all in mg kg⁻¹). All strains were affiliated to Pseudomonas spp. (except two), synthetised auxins and siderophores and solubilised P (except three), and seven of them were ACCd positive. The inoculation effects (shoot-root-nodule biomass, chlorophyll content) depended on legume species and bacterial strain genotype. Phytostimulation scores were unrelated to siderophore/auxin synthesis and P solubilisation rates. Inoculations of the strain nos. 17–43 triggered a 1.2-fold significant increase in the chlorophyll content of A. vulneraria. Chlorophyll content and root biomass of L. corniculatus were significantly increased following the inoculations of the strain nos. 17–22 (1.5–1.4-fold, respectively). The strongest positive effects were related to increases in the nodule biomass of L. corniculatus in the presence of three ACCd-positive strains (1.8-fold), one of which was the highest auxin producer. These data suggest to focus on interactions between ACCd activity and auxin synthesis to enhance nodulation of metallicolous legumes.

Copper (Cu) contamination of soils may alter the functioning and sustainability of vineyard ecosystems. Cultivating Cu-extracting plants in vineyard inter-rows, or phytoextraction, is one possible way currently under consideration in agroecology to reduce Cu contamination of vineyard topsoils. This option is rarely used, mainly because Cu phytoextraction yields are too low to significantly reduce contamination due to the relatively “low” phytoavailability of Cu in the soil (compared to other trace metals) and its preferential accumulation in the roots of most extracting plants. This article describes the main practices and associated constraints that could theoretically be used to maximize Cu phytoextraction at field scale, including the use of Cu-accumulating plants grown (i) with acidifying plants (e.g., leguminous plants), and/or (ii) in the presence of acidifying fertilizers (ammonium, elemental sulfur), or (iii) with soluble “biochelators” added to the soil such as natural humic substances or metabolites produced by rhizospheric bacteria such as siderophores, in the inter-rows. This discussion article also provides an overview of the possible ways to exploit Cu-enriched biomass, notably through ecocatalysis or biofortification of animal feed.

Heavy metal–resistant siderophore-producing bacteria (SPB) with plant growth–promoting traits can assist in phytoremediation of heavy metal–contaminated soil. We isolated siderophore-producing bacteria from Pb and Zn mine soil in Shangyu, Zhejiang, China. The isolate with the highest siderophore production, strain SX9, was identified as Burkholderia sp. Burkholderia sp. SX9 produced catecholate-type siderophore, with the highest production at a pH range of 6.0 to 8.0, a temperature range of 20 to 30 °C and NaCl concentration below 2%. Siderophore production was highest without Fe³⁺ and became gradually lower with increasing Fe³⁺ concentration. Minimal inhibitory concentrations (MIC) of Pb²⁺, Zn²⁺, Cu²⁺, and Cd²⁺ were 4000, 22000, 5000, and 2000 μmol L⁻¹, respectively. The strain had a strong metal solubilization ability: the contents of Cu²⁺, Zn²⁺, and Cd²⁺ in the supernatant were 47.4%, 133.0%, and 35.4% higher, respectively, in strain SX9–inoculated cultures than in the not inoculated controls. The siderophore produced by strain SX9 could combine with Fe³⁺, Zn²⁺, and Cd²⁺ with good effectiveness. The plant growth–promoting traits of the strain included indole acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and phosphate solubilization capability. Compared to the uninoculated growth medium and SX9 culture supernatant, the germination rate of Lolium perenne seeds was higher when inoculated with strain SX9 culture. In the experiment of seed germination, adding bacterial culture or supernatant could alleviate the toxicity of heavy metals to L. perenne seed germination. Under Cu²⁺ and Zn²⁺ stress, strain SX9 promoted the germination rate. Taken together, Burkholderia sp. SX9 had properties beneficial in the microbial enhancement of phytoremediation of soil contaminated with heavy metals.

Soil cadmium (Cd) contamination resulting from anthropogenic activity poses severe threats to food safety and human health. In this study, a pot experiment was performed to evaluate the possibility of using urease-producing bacterium UR21 and eggshell (ES) waste for improving the physiological characteristics and reducing Cd accumulation of pakchoi (Brassica chinensis L.) plants. UR21 has siderophore and IAA production ability. The application of UR21 and ES individually or in combination could improve the root and shoot length, and fresh and dry weight of pakchoi plants under Cd stress. In Cd + ES + UR21–treated plants, the dry weight of shoot and root were increased by 61.54% and 72.73%, respectively. The chlorophyll a, chlorophyll b, and carotenoid content were increased by 52.19%, 42.95%, and 95.56% in Cd + ES + UR21–treated plants. Meanwhile, the H₂O₂ and MDA content were decreased while the SOD and POD activity were increased, and an increase of soluble protein level in pakchoi plants was observed under Cd + ES + UR21 treatment. Importantly, eggshell and UR21 alone or in combination induced a decline of Cd content in pakchoi plants, especially that Cd + ES + UR21 treatment decreased Cd content in shoot and root by 26.96% and 42.91%, respectively. Meanwhile, the soil urease and sucrase activities were enhanced. Generally, the combined application of ureolytic bacteria UR21 and eggshell exhibited better effects than applied them individually in terms of alleviating Cd toxicity in pakchoi plants. Our findings may give a unique perspective for an eco-friendly and sustainable strategy to remediate heavy metal–polluted soils.