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X-ray absorption spectroscopy evidence of sulfur-bound Cadmium in the Cd-hyperaccumulator Solanum nigrum and the non-accumulator Solanum melongena
2021
Pons, Marie-Noëlle | Collin, Blanche | Doelsch, Emmanuel | Chaurand, Perrine | Fehlauer, Till | Levard, Clément | Keller, Catherine | Rose, Jérôme
It has been proposed that non-protein thiols and organic acids play a major role in cadmium phytoavailability and distribution in plants. In the Cd-hyperaccumulator Solanum nigrum and non-accumulator Solanum melongena, the role of these organic ligands in the accumulation and detoxification mechanisms of Cd are debated. In this study, we used X-ray absorption spectroscopy to investigate Cd speciation in these plants (roots, stem, leaves) and in the soils used for their culture to unravel the plants responses to Cd exposure. The results show that Cd in the 100 mg.kg-1 Cd-doped clayey loam soil is sorbed onto iron oxyhydroxides. In both S. nigrum and S. melongena, Cd in roots and fresh leaves is mainly bound to thiol ligands, with a small contribution of inorganic S ligands in S. nigrum leaves. We interpret the Cd binding to sulfur ligands as detoxification mechanisms, possibly involving the sequestration of Cd complexed with glutathione or phytochelatins in the plant vacuoles. In the stems, results show an increase binding of Cd to -O ligands (>50% for S. nigrum). We suggest that Cd is partly complexed by organic acids for transportation in the sap.
Show more [+] Less [-]Cadmium phytoavailability under greenhouse vegetable production system measured by diffusive gradients in thin films (DGT) and its implications for the soil threshold
2018
Tian, Kang | Xing, Zhe | Liu, Guoming | Wang, Huifeng | Jia, Mengmeng | Hu, Wenyou | Huang, Biao
The diffusive gradients in thin films (DGT) technique is recognized to have advantages over traditional techniques. For example, the passive measurement generally follows the principle of metal uptake by plants, and its result incorporates the influences of soil properties, which may make DGT a good protocol for improving soil quality guidelines (SQGs). However, DGT has rarely been applied to assess Cd phytoavailability in soils under greenhouse vegetable production (GVP) systems. In this study, 29 turnips (Raphanussativus L.), 21 eggplants (Solanum melongena L.) and their corresponding soils were collected from GVP systems in Dongtai and Shouguang, eastern China. Simple linear regression and stepwise regression were performed using the soil Cd content and soil properties to predict the vegetable Cd content. Soil thresholds were derived based on both total and available Cd concentrations. The results showed that total Cd, DGT-measured Cd (DGT-Cd), soil-solution Cd (Soln-Cd) and CaCl2-extractable Cd (CaCl2-Cd) were all significantly correlated with vegetable Cd. DGT-Cd had the best correlation with turnip Cd. The total Cd threshold values ranged from 4.87 (pH 6.5) to 5.18 (pH 7.5) mg kg−1 for turnips and 14.60 (pH 6.5) to 14.90 (pH 7.5) mg kg−1 for eggplants. These Cd thresholds were higher than the current SQGs. The predicted of turnip Cd by DGT-Cd was not improved significantly by further considering the soil properties. The calculated soil threshold of DGT-Cd was 5.35 μg L−1 for turnips. However, the predicted soil threshold of DGT-Cd for eggplant was improved by including SOM, with R2 values from 0.53 to 0.70. The DGT-Cd threshold was calculated as 1.81 μg L−1 for eggplant (30.0 g kg−1 SOM). In conclusion, whether DGT measurements are independent of soil properties and preferable for the evaluation of Cd phytoavailability and the generation of soil thresholds remains to be clarified in future research.
Show more [+] Less [-]X-ray absorption spectroscopy evidence of sulfur-bound cadmium in the Cd-hyperaccumulator Solanum nigrum and the non-accumulator Solanum melongena
2021
Pons, Marie-Laure | Collin, Blanche | Doelsch, Emmanuel | Chaurand, Perrine | Fehlauer, Till | Levard, Clément | Keller, Catherine | Rose, Jerome
It has been proposed that non-protein thiols and organic acids play a major role in cadmium phytoavailability and distribution in plants. In the Cd-hyperaccumulator Solanum nigrum and non-accumulator Solanum melongena, the role of these organic ligands in the accumulation and detoxification mechanisms of Cd are debated. In this study, we used X-ray absorption spectroscopy to investigate Cd speciation in these plants (roots, stem, leaves) and in the soils used for their culture to unravel the plants responses to Cd exposure. The results show that Cd in the 100 mg kg⁻¹ Cd-doped clayey loam soil is sorbed onto iron oxyhydroxides. In both S. nigrum and S. melongena, Cd in roots and fresh leaves is mainly bound to thiol ligands, with a small contribution of inorganic S ligands in S. nigrum leaves. We interpret the Cd binding to sulfur ligands as detoxification mechanisms, possibly involving the sequestration of Cd complexed with glutathione or phytochelatins in the plant vacuoles. In the stems, results show an increase binding of Cd to –O ligands (>50% for S. nigrum). We suggest that Cd is partly complexed by organic acids for transportation in the sap.
Show more [+] Less [-]Selection for low-cadmium cultivars and cadmium subcellular distribution comparison between two selected cultivars of eggplant (Solanum melongena L.)
2021
Shen, Chuang | Fu, Huiling | Liao, Qiong | Huang, Bai-Fei | Huang, Ying-Ying | Xin, Jun-Liang
Excessive accumulation of cadmium (Cd) in vegetables poses a serious threat to human health; therefore, it is urgent to screen and cultivate vegetable cultivars with low Cd accumulation in the edible parts. Eggplant has a high tendency for Cd accumulation, but research on its low Cd accumulation cultivars is still rare. In this study, to screen low-Cd cultivars, 30 eggplant cultivars were screened using soils containing 0.22 mg/kg, 2.9 mg/kg (low-Cd), and 4.7 mg/kg of Cd (high-Cd). MYCQ and ZGQ were confirmed as low-Cd cultivars, BXGZ and WCCQ were confirmed as high-Cd cultivars, and a 2.52–3.88-fold difference in Cd concentration was observed in their fruits. The subcellular distribution revealed that the root cell wall and vacuole Cd concentrations of a typical low-Cd cultivar (MYCQ) were significantly higher than those of a typical high-Cd cultivar (BXGZ); however, the Cd concentrations in the cell wall and vacuole in fruits, leaves, and stems were significantly lower in MYCQ than in BXGZ. These results indicated that the low-Cd cultivars of eggplant could lessen Cd toxicity through the elevated Cd retention and sequestration levels of root cell walls and vacuoles, thus reducing Cd transport from roots to aboveground tissues, leading to low Cd accumulation. The findings of this study can provide a physiological and biochemical foundation for the screening and breeding of low-Cd cultivars of fruit vegetables and demonstrates that the application of low-Cd cultivars is necessary for food safety in humans.
Show more [+] Less [-]Dietary intake assessment of pyrethroid residues from okra and eggplant grown in peri-urban areas of Punjab, Pakistan
2020
Amjad, Adnan | Randhawa, Muhammad Atif | Javed, Muhammad Sameem | Muhammad, Zafarullah | Ashraf, Mussawar | Ahmad, Zulfiqar | Murtaza, Shamas
This study was designed to assess the gradual increase in the use of insecticides on vegetables and to familiarize the consumers regarding the insecticide residues. The purpose of this research work was to highlight the detrimental effects of pyrethroids (bifenthrin, cyfluthrin, cypermethrin, deltamethrin, fenvalerate, lambda-cyhalothrin, and permethrin) compare with dietary intake assessment of eggplant and okra grown in peri-urban environment. In this manner, a total of 180 (n = 60 × 3) samples of eggplant (Solanum melongena) and okra (Abelmoschus esculentus) were procured from the peri-urban farming system of Faisalabad, Multan, and Gujranwala to assess the pyrethroid residues along with their dietary intake assessment. The procured vegetables were quantified for pyrethroid residues by using gas chromatography (GC) equipped with an electron capture detector (ECD). Outcomes of this study revealed that for okra samples, the highest residues of bifenthrin (1.25 mg kg⁻¹) were found in Gujranwala then Multan (1.5 mg kg⁻¹) and Faisalabad (1.04 mg kg⁻¹), whereas in eggplant, the highest residues were recorded for bifenthrin from Faisalabad (1.33 mg kg⁻¹) and Gujranwala (0.78 mg kg⁻¹). In Multan, the highest residues for cyfluthrin (1.18 mg kg⁻¹) were reported in eggplant. Out of all analyzed samples for pyrethroid residues, 32% samples contained detectable residues and 6% samples exceeded their maximum residual limits (MRLs) established by the European Union (EU). Dietary intake assessment (mg kg⁻¹ day⁻¹) was calculated as per their maximum permissible intake (MPI) values, i.e., bifenthrin (1.28), cyfluthrin (1.28), cypermethrin (3.20), deltamethrin (0.64), fenvalerate (1.28), lambda-cyhalothrin (0.064), and permethrin (3.20) respectively. Conclusively, residues from the Multan region were greater than those from Gujranwala and Faisalabad showing excessive application of pyrethroids. Overall results revealed that although some samples exceeded MRLs in selected areas, their safe consumption limit was found.
Show more [+] Less [-]Concentrations, Accumulation, and Interactions of Redoximorphic Metals (Fe, Mn) Between Other Elements in Plants Grown on Wastewater-Irrigated and Control Soils
2014
Avci, Halim | Yaman, Mehmet
Samples of soil and food plants were collected from wastewater-irrigated and control fields in the vicinity of Gaziantep, in southeast Turkey. The samples were analyzed for concentrations of several macro and trace elements to evaluate spatial differences and bioaccumulation. Emphasis was placed on redoximorphic metal (Mn/Fe) interactions. The plants and tissues that studied were corn (Zea mays) seeds, mint (Mentha) leaves, the vegetables eggplant (Solanum melongena L.) and pepper (Capsicum annuum L.), and tomato (Solanum lycopersicum L.) fruits. Concentrations of Mn and Fe in corn were generally lower than in the other food plants, while concentrations of Mn, Fe, and several elements in mint were higher in other plants. Except for mint, the Mn deficiencies in the various plant samples can be attributed to low Mn soil concentrations and the chemical and physical characteristics of the soil. Mn concentrations in both wastewater-irrigated soils and control soils were lower than what has been reported as an average for the Earth’s crust (crustal average). There was considerable variability in the concentrations of Fe, with mint having the highest concentration (650 mg/kg) and corn the lowest (20 mg/kg). Significant positive relationships (coefficient of determination (R²) >0.50) were calculated between Mn and Fe in corn (R² = 0.83). The R²for tomato was 0.43, but all other relationships were much poorer for all other species. Several elements (trace and macro) demonstrated positive relationships with Mn or Fe, although there was little across-species consistency. For example, the R²values for both Mn and Fe correlated with Zn, P, and Mg were all >0.80 for Z. mays, but were all <0.10 for Mentha. The response of the members of the Solanaceae family (eggplant, pepper, and tomato) to the presence of Mn, Fe, and other soil constituents was similar in many respects, showing differences from Z. mays and, in particular, Mentha. Similarities among related plants are not surprising and would be expected given similar physiologies and metabolic pathways. Higher uptake of certain metals may be associated with the dominant form of the element in the soil matrix. The uptake of chemicals to plant tissues is influenced by the chemical and physical characteristics of the soil and species-specific factors.
Show more [+] Less [-]Metal translocation patterns in Solanum melongena grown in close proximity to traffic
2014
Wiseman, Clare L. S. | Zereini, Fathi | Püttmann, Wilhelm
The purpose of this study is to examine tissue patterns of metal (Cr, Ni, Cu, Cd, and Pb) concentrations in Solanum melongena cultivated in close proximity to traffic to help elucidate associated elemental deposition and soil-to-root and root-to-shoot transfers. Plants were cultivated in a commercial soil mix at three sites in Toronto, Canada. Metal concentrations were determined on microwave-digested bulk and rhizosphere soil and tissue samples per ICP-MS, along with two standard reference materials (NIST #1570a and #2709a). Unwashed and washed S. melongena samples were also analyzed, along with Origanum vulgare plants from the same sites, to assess the effectiveness of washing in reducing metal concentrations. The tissue distribution of Cr, Ni, Cu, and Pb demonstrated variability as a function of traffic proximity. Copper was found to easily translocate to roots in soils susceptible to waterlogging, while Cd had the highest soil-to-root and root-to-shoot translocation. The translocation of Cd was highest at the roadside site, due to a greater relative enrichment of this metal in the rhizosphere of S. melongena plants. Washing O. vulgare leaves was more effective in removing metal-associated particles compared to S. melongena samples. Cadmium uptake is of greatest concern given its toxicity and translocation potential.
Show more [+] Less [-]Assessment of heavy metals contamination and the risk of non-cancerous diseases in vegetable using electromagnetic-chemical
2020
Mokarram, Marzieh | Amin, Hosein | Setoodeh, Abdollah
This study seeks to assess the reaction of the eggplant (Solanum melongena L.) to soil samples contaminated. Following, cultivation, growth, and harvest, the plant samples were prepared and maximum absorption rates of heavy metals were measured in both leaf and fruit. The estimated daily intake (EDI), the target hazard quotient (THQ), and the bio-concentration factor (BCF) were measured at various intervals during the growth period of the plant. Spectral analysis was also performed to assess the reaction of target crops to heavy metals. The results showed that in the second and third stages of plant growth, the THQ values were more than 1 for infected plants with Cd, Pb, and Zn. According to results from the BCF analysis, the absorption rate in Pb, during the growth stages was relatively high, in crops contaminated by Ni was around 1 in the second and third stages, and in plants contaminated by Cd was extremely high. All crops contaminated by heavy metals showed higher reflection rates in the 400–500 and 600–700 nm range. So, using electromagnetic waves during different stages of growth, the reaction of eggplant cultivated in soil samples contaminated by heavy metals is predictable.
Show more [+] Less [-]Dissipation of deltamethrin, triazophos, and endosulfan in ready mix formulations in tomato (Lycopersicon esculentum L.) and Egg plant (Solanum melongena L.)
2015
Mukherjee, Irani | Kumar, Ashok | Kumar, Aman
Persistence of delltamethrin, endosulfan, and triazophos in egg plant and tomato was studied following application of two ready mix formulations of insecticides viz. deltametrhin and endosulfan (Cobra 5000; 0.75 % deltamethrin + 29.5 % endosulfan) and deltamethrin and triazophos (Annaconda Plus; 1 % deltamethrin + 35 % triazophos) at recommended (1.0 L/ha and double dose 2.0 L/ha). The residues of deltamethrin persisted till 7 and 5 days in tomato and egg plant fruits, respectively, in the ready mix formulation of Cobra 5000 whereas endosulfan persisted till 15 and 10 days in tomato and egg plant fruits, respectively. Dissipation of the insecticides followed first-order kinetics with half-life values of deltamethrin and endosulfan ranged from 2.6 to 4.7 and 1.4 to 1.7 days, respectively, for both the vegetables. In case of combination mix of deltamethrin and triazophos (Annaconda Plus), deltamethrin persisted beyond 5 days in both tomato and egg plant fruits, while triazophos persisted till 10 days in both the vegetables. Residues of deltamethrin and triazophos dissipated with half-life of 2.6–4.2 and 1.7–4.1 days, respectively, on tomato and egg plant fruits. Based on the Codex MRL limits, a safe waiting period of 5 and 3 days is suggested for tomato and egg plant, respectively, for the ready mix formulation of deltamethrin and endosulfan (Cobra 5000), and 5-day waiting period is suggested for tomato and egg plant for the combination mix of deltamethrin and triazophos.
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