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Vulnerability and tolerance to nickel of periphytic biofilm harvested in summer and winter Full text
2022
Laderriere, Vincent | Morin, Soizic | Eon, Mélissa | Fortin, Claude | Centre Eau Terre Environnement [Québec] (INRS - ETE) ; Institut National de la Recherche Scientifique [Québec] (INRS) | Ecosystèmes aquatiques et changements globaux (UR EABX) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Nunavik
International audience | Metals are naturally present in freshwater ecosystems but anthropogenic activities like mining operations represent a long-standing concern. Metals released into aquatic environments may affect microbial communities such as periphytic biofilm, which plays a key role as a primary producer in stream ecosystems. Using two 28-day microcosm studies involving two different photoperiods (light/dark cycle of 16/8 vs 8/16), the present study assessed the effects of four increasing nickel (Ni) concentrations (0-6 mu M) on two natural biofilm communities collected at different seasons (summer and winter). The two communities were characterized by different structural profiles and showed significant differences in Ni accumulated content for each treatment. For instance, the biofilm metal content was four times higher in the case of summer biofilm at the highest Ni treatment and after 28 days of exposure. Biomarkers examined targeted both heterotrophic and autotrophic organisms. For heterotrophs, the 8-glucosidase and 8-glucosaminidase showed no marked effects of Ni exposure and were globally similar between the two communities suggesting low toxicity. However, the photosynthetic yield confirmed the toxicity of Ni on autotrophs with maximum inhibition of 81 +/- 7% and 60 +/- 1% respectively for the summer and winter biofilms. Furthermore, biofilms previously exposed to the highest long-term Ni con-centration ([Ni2+] = 6 mu M) revealed no acute effects in subsequent toxicity based on the PSII yield, suggesting a tolerance acquisition by the phototrophic community. Taken together, the results suggest that the biofilm response to Ni exposure was dependent of the function considered and that descriptors such as biofilm metal content could be seasonally dependent, information of great importance in a context of biomonitoring.
Show more [+] Less [-]Contrasted fate of zinc sulfide nanoparticles in soil revealed by a combination of X-ray absorption spectroscopy, diffusive gradient in thin films and isotope tracing Full text
2022
Bars, Maureen Le | Legros, Samuel | Levard, Clément | Chevassus-Rosset, Claire | Montes, Mélanie | Tella, Marie | Borschneck, Daniel | Guihou, Abel | Angeletti, Bernard | Doelsch, Emmanuel | Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement (CEREGE) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Recyclage et risque (UPR Recyclage et risque) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | ANR-15-CE34-0003,DIGESTATE,Diagnostic des traitements des déchets et comportement des contaminants dans l'environnement(2015) | European Project: 795614,Marie Skodowska-Curie agreement
Partie B | International audience | Incidental zinc sulfide nanoparticles (nano-ZnS) are spread on soils through organic waste (OW) recycling. Here we performed soil incubations with synthetic nano-ZnS (3 nm crystallite size), representative of the form found in OW. We used an original set of techniques to reveal the fate of nano-ZnS in two soils with different properties. 68 Zn tracing and nano-DGT were combined during soil incubation to discriminate the available natural Zn from the soil, and the available Zn from the dissolved nano-68 ZnS. This combination was crucial to highlight the dissolution of nano-68 ZnS as of the third day of incubation. Based on the extended X-ray absorption fine structure, we revealed faster dissolution of nano-ZnS in clayey soil (82% within 1 month) than in sandy soil (2% within 1 month). However, the nano-DGT results showed limited availability of Zn released by nano-ZnS dissolution after 1 month in the clayey soil compared with the sandy soil. These results highlighted: (i) the key role of soil properties for nano-ZnS fate, and (ii) fast dissolution of nano-ZnS in clayey soil. Finally, the higher availability of Zn in the sandy soil despite the lower nano-ZnS dissolution rate is counterintuitive. This study demonstrated that, in addition to nanoparticle dissolution, it is also essential to take the availability of released ions into account when studying the fate of nanoparticles in soil.
Show more [+] Less [-]X-ray absorption spectroscopy evidence of sulfur-bound cadmium in the Cd-hyperaccumulator Solanum nigrum and the non-accumulator Solanum melongena Full text
2021
Pons, Marie-Laure | Collin, Blanche | Doelsch, Emmanuel | Chaurand, Perrine | Fehlauer, Till | Levard, Clément | Keller, Catherine | Rose, Jérôme | Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement (CEREGE) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Recyclage et risque (UPR Recyclage et risque) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)
International audience | It has been proposed that non-protein thiols and organic acids play a major role in cadmium phytoavailability and distribution in plants. In the Cd-hyperaccumulator Solanum nigrum and non-accumulator Solanum melongena, the role of these organic ligands in the accumulation and detoxification mechanisms of Cd are debated. In this study, we used X-ray absorption spectroscopy to investigate Cd speciation in these plants (roots, stem, leaves) and in the soils used for their culture to unravel the plants responses to Cd exposure. The results show that Cd in the 100 mg.kg-1 Cd-doped clayey loam soil is sorbed onto iron oxyhydroxides. In both S. nigrum and S. melongena, Cd in roots and fresh leaves is mainly bound to thiol ligands, with a small contribution of inorganic S ligands in S. nigrum leaves. We interpret the Cd binding to sulfur ligands as detoxification mechanisms, possibly involving the sequestration of Cd complexed with glutathione or phytochelatins in the plant vacuoles. In the stems, results show an increase binding of Cd to -O ligands (>50% for S. nigrum). We suggest that Cd is partly complexed by organic acids for transportation in the sap.
Show more [+] Less [-]Long-term fate of exogenous metals in a sandy Luvisol subjected to intensive irrigation with raw wastewater Full text
2007
Dère, Christelle | Lamy, Isabelle | Jaulin, Anne, A. | Cornu, Sophie, S. | Unité de recherche Science du Sol (USS) ; Institut National de la Recherche Agronomique (INRA) | Unité de Science du Sol ; Institut National de la Recherche Agronomique (INRA)
International audience | From 1899 to 2002, sandy Luvisol in the Paris region has been intensively irrigated with raw wastewater, resulting in major soil pollution by metallic trace elements (MTE). To identify the soil phases implicated in retaining these metals, sequential extractions were performed on a solum irrigated with untreated wastewater and another reference solum. The endogenous and exogenous fractions of MTE in the contaminated soil were discriminated using correlations between MTE and major elements defined from unpolluted soils of the area. In the contaminated soil no exogenous lead and chromium are present below the surface horizon, whereas exogenous zinc and copper are found down to the base of the solum. The endogenous MTE are mainly found in the residual fraction. Exogenous MTE appear to be associated with organic matter in the surface horizon, and exogenous zinc seems to be readsorbed on iron and manganese oxyhydroxides in the underlying horizons. After 100 years of intensive irrigation with wastewater, no exogenous Pb and Cr are found in the subsoil, while exogenous Zn and Cu are found down to the base of the solum, mostly readsorbed.
Show more [+] Less [-]Effect of pyoverdine supply on cadmium and nickel complexation and phytoavailability in hydroponics Full text
2015
Ferret, C. | Cornu, J. | Elhabiri, M. | Sterckeman, Thibault | Braud, A. | Jezequel, K. | Lollier, M. | Lebeau, T. | Schalk, I. | Geoffroy, V. | Service d'Hépato-gastro-entérologie et Nutrition [CHU Dupuytren 1, Limoges] ; CHU Limoges-Hôpital Dupuytren [CHU Limoges] | Laboratoire de chimie moléculaire (LCM) ; Université de Strasbourg (UNISTRA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Sols et Environnement (LSE) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG) ; Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) ; Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Vigne Biotechnologie et Environnement (LVBE) ; Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)) | Equipe Dépollution Biologique des Sols ; Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))
Effect of pyoverdine supply on cadmium and nickel complexation and phytoavailability in hydroponics Full text
2015
Ferret, C. | Cornu, J. | Elhabiri, M. | Sterckeman, Thibault | Braud, A. | Jezequel, K. | Lollier, M. | Lebeau, T. | Schalk, I. | Geoffroy, V. | Service d'Hépato-gastro-entérologie et Nutrition [CHU Dupuytren 1, Limoges] ; CHU Limoges-Hôpital Dupuytren [CHU Limoges] | Laboratoire de chimie moléculaire (LCM) ; Université de Strasbourg (UNISTRA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Sols et Environnement (LSE) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG) ; Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) ; Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Vigne Biotechnologie et Environnement (LVBE) ; Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)) | Equipe Dépollution Biologique des Sols ; Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))
International audience | Siderophores are chelators with a high selectivity for Fe(III) and a good affinity for divalent metals, including Cd(II) and Ni(II). Inoculation with siderophore-producing bacteria (SPB) has thus been proposed as an alternative to chelator supply in phytoremediation. Accurate assessments of the potential of this association require a dissection of the interaction of siderophores with metals at the soil–root interface. This study focuses on pyoverdine (Pvd), the main siderophore produced by Pseudomonas aeruginosa. We first assessed the ability of Pvd to coordinate Ni(II). The stability constant of Pvd–Ni(II) (log K L′Ni = 10.9) was found to be higher than that of Pvd–Cd(II) (log K L′Cd = 8.2). We then investigated the effect of a direct supply of Pvd on the mobilization, speciation, and phytoavailability of Cd and Ni in hydroponics. When supplied at a concentration of 50 μM, Pvd selectively promoted Ni mobilization from smectite. It decreased plant Ni and Cd contents and the free ionic fractions of these two metals, consistent with the free ion activity model. Pvd had a more pronounced effect for Ni than for Cd, as predicted from its coordination properties. Inoculation with P. aeruginosa had a similar effect on Ni phytoavailability to the direct supply of Pvd.
Show more [+] Less [-]Effect of pyoverdine supply on cadmium and nickel complexation and phytoavailability in hydroponics Full text
2015
Ferret, C. | Cornu, J. Y. | Elhabiri, M. | Sterckeman, T. | Braud, A. | Jezequel, K. | Lollier, M. | Lebeau, T. | Schalk, I. J. | Geoffroy, V. A.
Siderophores are chelators with a high selectivity for Fe(III) and a good affinity for divalent metals, including Cd(II) and Ni(II). Inoculation with siderophore-producing bacteria (SPB) has thus been proposed as an alternative to chelator supply in phytoremediation. Accurate assessments of the potential of this association require a dissection of the interaction of siderophores with metals at the soil–root interface. This study focuses on pyoverdine (Pvd), the main siderophore produced by Pseudomonas aeruginosa. We first assessed the ability of Pvd to coordinate Ni(II). The stability constant of Pvd–Ni(II) (log K ᴸ′ᴺⁱ = 10.9) was found to be higher than that of Pvd–Cd(II) (log K ᴸ′Cᵈ = 8.2). We then investigated the effect of a direct supply of Pvd on the mobilization, speciation, and phytoavailability of Cd and Ni in hydroponics. When supplied at a concentration of 50 μM, Pvd selectively promoted Ni mobilization from smectite. It decreased plant Ni and Cd contents and the free ionic fractions of these two metals, consistent with the free ion activity model. Pvd had a more pronounced effect for Ni than for Cd, as predicted from its coordination properties. Inoculation with P. aeruginosa had a similar effect on Ni phytoavailability to the direct supply of Pvd.
Show more [+] Less [-]Implications of speciation on rare earth element toxicity: a focus on organic matter influence in Daphnia magna standard test Full text
2022
Lachaux, Nicolas | Catrouillet, Charlotte | Marsac, Remi | Poirier, Laurence | Pain-Devin, Sandrine | Gross, Elisabeth Maria | Giamberini, Laure | Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC) ; Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo) ; Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) | Institut de Physique du Globe de Paris (IPGP (UMR_7154)) ; Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) | Géosciences Rennes (GR) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des sciences de l'environnement de Rennes (OSERen) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut Des Substances et Organismes de la Mer - UR 2160 (ISOMER) ; Nantes Université - UFR des Sciences Pharmaceutiques et Biologiques (Nantes Univ - UFR Pharmacie) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST) ; Nantes Université - pôle Sciences et technologie ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie ; Nantes Université (Nantes Univ)
International audience | Rare earth elements (REE) have become essential in high- and green-technologies. Their increasing use lead to the release of anthropogenic REE into the environment including aquatic systems. The limited data available on the aquatic ecotoxicology of REE indicate their biological effects are highly dependent on their speciation, posing challenges for a reliable environmental risk assessment (ERA). The current study assessed the influence of speciation on the toxicity of neodymium (Nd), gadolinium (Gd) and ytterbium (Yb) in the Daphnia magna mobility inhibition test (ISO 6341:2012). REE toxicity was assessed individually and in ternary mixture, in the absence and presence of dissolved organic matter (DOM). Speciation was predicted by modeling and REE bioaccumulation by D. magna was measured to better understand the relationship between REE speciation and toxicity. DOM decreased significantly the toxicity of Nd, Gd and the mixture towards this freshwater crustacean. This was explained by a lower REE bioaccumulation in the presence of DOM due to REE-DOM complexation, which reduced REE bioavailability. DOM effects on Yb toxicity and bioaccumulation were limited because of Yb precipitation. We show that the way of expressing EC50 values (based on nominal, measured or predicted REE concentrations in solution) drastically changed REE toxicity assessment and that these changes were influenced by REE speciation. This study demonstrates for the first time that REE speciation, and especially REE-DOM complexation, significantly influences REE bioaccumulation and toxicity towards D. magna. Our results have implications for the subsequent ERA of REE.
Show more [+] Less [-]Spatial and temporal distribution of mercury and methylmercury in bivalves from the French coastline Full text
2017
Briant, Nicolas | Chouvelon, Tiphaine | Martinez, Laura | Brach-papa, Christophe | Chiffoleau, Jean-francois | Savoye, N. | Sonke, J. | Knoery, Joel
Marine mercury (Hg) concentrations have been monitored in the French coastline for the last half a century using bivalves. The analyses presented in this study concerned 192 samples of bivalves (mussels: Mytilus edulis and Mytilus galloprovincialis and oysters: Crassostrea gigas and Isognomon alatus) from 77 sampling stations along the French coast and in the French Antilles sea. The goals of this study were to assess MeHg levels in various common bivalves from French coastline, and to identify possible geographic, taxonomic or temporal variations of concentrations. We show that the evolution of methylmercury (MeHg) concentrations covary with total mercury (HgT) concentrations. Moreover, in most of the study sites, HgT concentrations have not decreased since 1987, despite regulations to decrease or ban mercury used for anthropic activities.
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