Processes of transformation of organic matter are slowed down so that cellulose which originates from cell walls of Sphagnum is retained long in unchanged form. The highest valises of cellulolytic bacteria (0.33-126.67 bact/ml) were recorded in October. In most cases slightly active species were found, Cellvibrio fulvus was dominant. The participation of fungi in cellulolytic community was significant 23-31.5.

We investigated the variation of N:P and N:K ratio in ombrotrophic Sphagnum plants along a gradient of atmospheric N deposition from 1 to 2.5 g m⁻² year⁻¹ in Central-East Europe. The N:P and N:K ratio in Sphagnum capitula increased significantly along the N deposition gradient. Sphagnum species from the Cuspidata section were characterised by significantly lower ratios at low N deposition. When we compared the observed N:P ratios in Sphagnum plants with the values reported in a previous European-wide study, we found a correspondence in nutrient stoichiometry only for a few bogs: higher P concentration in Sphagnum capitula caused a lower N:P ratio in most of the study bogs so that Sphagnum plants still seem N-limited despite their N saturation. Interaction between summer water table decrease and aerial liming of surrounding forests is proposed as an explanation for this discrepancy. Local forestry practice interacting with climate thus alter N:P stoichiometry of Sphagnum along the N deposition gradient.

Trace elements (TEs) transported by atmospheric fluxes can negatively impact isolated ecosystems. Modelling based on moss-borne TE accumulation makes tracking TE deposition in remote areas without monitoring stations possible. Using a single moss species from ombrotrophic hummock peatlands reinforces estimate quality. This study used a validated geomatic model of particulate matter dispersion to identify the origin of Cd, Zn, Pb and Cu accumulated in Sphagnum capillifolium and the distance transported from their emission sources. The residential and industrial sectors of particulate matter emissions showed the highest correlations with the TEs accumulated in S. capillifolium (0.28(Zn)-0.56(Cu)) and (0.27(Zn)-0.47(Cu), respectively). Distances of dispersion varied depending on the sector of emissions and the considered TE. The greatest transportation distances for mean emissions values were found in the industrial (10.6 km when correlating with all TEs) and roads sectors (13 km when correlating with Pb). The residential sector showed the shortest distances (3.6 km when correlating with Cu, Cd, and Zn). The model presented here is a new tool for evaluating the efficacy of air pollution abatement policies in non-monitored areas and provides high-resolution (200 × 200 m) maps of TE accumulation that make it possible to survey the potential impacts of TEs on isolated ecosystems.

To quantify potential nitrogen (N) deposition impacts on peatland carbon (C) uptake, we explored temporal and spatial trends in N deposition and climate impacts on the production of the key peat forming functional group (Sphagnum mosses) across European peatlands for the period 1900–2050. Using a modelling approach we estimated that between 1900 and 1950 N deposition impacts remained limited irrespective of geographical position. Between 1950 and 2000 N deposition depressed production between 0 and 25% relative to 1900, particularly in temperate regions. Future scenarios indicate this trend will continue and become more pronounced with climate warming. At the European scale, the consequences for Sphagnum net C-uptake remained small relative to 1900 due to the low peatland cover in high-N areas. The predicted impacts of likely changes in N deposition on Sphagnum productivity appeared to be less than those of climate. Nevertheless, current critical loads for peatlands are likely to hold under a future climate.

Central European mountain bogs, among the most valuable and threatened of habitats, were exposed to intensive human impact during the 20th century. We reconstructed the subrecent water chemistry and water-table depths using diatom based transfer functions calibrated from modern sampling. Herbarium Sphagnum specimens collected during the period 1918–1998 were used as a source of historic diatom samples. We classified samples into hummocks and hollows according to the identity of dominant Sphagnum species, to reduce bias caused by uneven sampling of particular microhabitats. Our results provide clear evidence for bog pollution by grazing during the period 1918–1947 and by undocumented aerial liming in the early 90-ies. We advocate use of herbarized epibryon as a source of information on subrecent conditions in recently polluted mires.

Natural media such as soil and sediment contain mineralogical and organic components with distinct chemical, surface, and electrostatic properties. To better understand the role of various soil and sediment components on particle transport, columns were packed with quartz sand and natural sediment with added Fe oxyhydroxide coating, illite clay, and peat moss to investigate how these added components influence nTiO₂ retention and transport in geochemically heterogeneous medium. Results showed that nTiO₂ transport was low at pH 5, attributable to the electrostatic attraction between positively-charged nTiO₂ and negatively-charged medium. While illite did not notably affect nTiO₂ transport at pH 5, Fe oxyhydroxide coating increased nTiO₂ transport due to electrostatic repulsion between Fe oxyhydroxide and nTiO₂. Peat moss also increased nTiO₂ transport at pH 5, attributable to the increased DOC concentration, which resulted in higher DOC adsorption to nTiO₂ and intensified electrostatic repulsion between nTiO₂ and the medium. At pH 9, nTiO₂ transport was high due to the electrostatic repulsion between negatively-charged nTiO₂ and medium surfaces. Fe oxyhydroxide coating at pH 9 slightly delayed nTiO₂ transport due to electrostatic attraction, while illite clay and peat moss substantially inhibited nTiO₂ transport via straining/entrapment or electrostatic attraction. Overall, this study demonstrated that pH has a considerable effect on how minerals and organic components of a medium influence nTiO₂ transport. At low pH, electrostatic attraction was the dominant mechanism, therefore, nTiO₂ mobility was low regardless of the differences in mineralogical and organic components. Conversely, nTiO₂ mobility was high at high pH and nTiO₂ retention was dominated by straining/entrapment and sensitive to the mineralogical and organic composition of the medium.

Little information exists concerning the long-term interactive effect of nitrogen (N) addition with phosphorus (P) and potassium (K) on Sphagnum N status. This study was conducted as part of a long-term N manipulation on Whim bog in south Scotland to evaluate the long-term alleviation effects of phosphorus (P) and potassium (K) on N saturation of Sphagnum (S. capillifolium). On this ombrotrophic peatland, where ambient deposition was 8 kg N ha−1 yr−1, 56 kg N ha−1 yr−1 of either ammonium (NH4+, Nred) or nitrate (NO3−, Nox) with and without P and K, were added over 11 years. Nutrient concentrations of Sphagnum stem and capitulum, and pore water quality of the Sphagnum layer were assessed. The N-saturated Sphagnum caused by long-term (11 years) and high doses (56 kg N ha−1 yr−1) of reduced N was not completely ameliorated by P and K addition; N concentrations in Sphagnum capitula for Nred 56 PK were comparable with those for Nred 56, although N concentrations in Sphagnum stems for Nred 56 PK were lower than those for Nred 56. While dissolved inorganic nitrogen (DIN) concentrations in pore water for Nred 56 PK were not different from Nred 56, they were lower for Nox 56 PK than for Nox 56 whose stage of N saturation had not advanced compared to Nred 56. These results indicate that increasing P and K availability has only a limited amelioration effect on the N assimilation of Sphagnum at an advanced stage of N saturation. This study concluded that over the long-term P and K additions will not offset the N saturation of Sphagnum.

Although a large body of literature exists on the use of transplanted mosses for biomonitoring of air pollution, no article has addressed so far the use and the accumulation performance of a cloned moss for this purpose. In this work, a direct comparison of metal accumulation between bags filled with a Sphagnum palustre L. clone or with native Pseudoscleropodium purum Hedw., one of the most used moss species in biomonitoring surveys, was investigated. The test was performed in sites with different atmospheric contamination levels selected in urban, industrial, agricultural and background areas of Italy and Spain. Among the eighteen elements investigated, S. palustre was significantly enriched in 10 elements (Al, Ba, Cr, Cu, Fe, Hg, Pb, Sr, V and Zn), while P. purum was enriched only in 6 elements (Al, Ba, Cu, Hg, Pb and Sr), and had a consistently lower uptake capacity than S. palustre. The clone proved to be more sensitive in terms of metal uptake and showed a better performance as a bioaccumulator, providing a higher accumulation signal and allowing a finer distinction among the different land uses and levels of pollution. The excellent uptake performance of the S. palustre clone compared to the native P. purum and its low and stable baseline elemental content, evidenced in this work, are key features for the improvement of the moss bag approach and its large scale application.

This paper presents the results of an experiment carried out for the first time in situ to select a treatment to devitalize mosses for use in active biomonitoring of water pollution. Three devitalizing treatments for the aquatic moss Fontinalis antipyretica were tested (i.e. oven-drying at 100 °C, oven-drying with a 50-80-100 °C temperature ramp, and boiling in water), and the effects of these on loss of material during exposure of the transplants and on the accumulation of different heavy metals and metalloids were determined. The suitability of using devitalized samples of the terrestrial moss Sphagnum denticulatum to biomonitor aquatic environments was also tested. The structure of mosses was altered in different ways by the devitalizing treatments. Devitalization by boiling water led to significantly less loss of material (p < 0.01) than the oven-drying treatments. However, devitalization by oven-drying with a temperature ramp yielded more stable results in relation to both loss of material and accumulation of elements. With the aim of standardizing the moss bag technique, the use of F. antipyretica devitalized by oven-drying with a temperature ramp is recommended, rather than other devitalization treatments or use of S. denticulatum.

The objective of this research was to assess factors controlling peat and plant chemistry, and vegetation composition in 18 peatlands surrounding Sudbury after more than 30 years of large (>95%) pollution emission reductions. Sites closer to the main Copper Cliff smelter had more humified peat and the surface horizons were greatly enriched in copper (Cu) and nickel (Ni). Copper and Ni concentrations in peat were significantly correlated with that in the plant tissue of Chamaedaphne calyculata. The pH of peat was the strongest determining factor for species richness, diversity, and community composition, although percent vascular plant cover was strongly negatively correlated with surface Cu and Ni concentrations in peat. Sphagnum frequency was also negatively related to peat Cu and Ni concentrations indicating sites close to Copper Cliff smelter remain adversely impacted by industrial activities.