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Study of aquatic life criteria and ecological risk assessment for triclocarban (TCC)
2019
Fan, Bo | Li, Ji | Wang, Xiaonan | Gao, Xiangyun | Chen, Jin | Ai, Shunhao | Li, Wenwen | Huang, Yun | Liu, Zhengtao
Triclocarban (TCC) is used as a broad-spectrum antimicrobial agent, the intensive detection of TCC in aquatic environments and its potential risks to aquatic organisms are concerned worldwide. In this study, 8 Chinese resident aquatic organisms from 3 phyla and 8 families were used for the toxicity tests, and four methods were employed to derive the aquatic life criteria (ALC). A criterion maximum concentration (CMC) of 1.46 μg/L and a criterion continuous concentration (CCC) of 0.21 μg/L were derived according to the USEPA guidelines. The acute predicted no effect concentrations (PNECs) derived by species sensitivity distribution (SSD) methods based on log-normal, log-logistic and Burr Type Ⅲ models were 2.64, 1.88 and 3.09 μg/L, respectively. The comparisons of ALCs derived with resident and non-resident species showed that the CMC and CCC of TCC derived with Chinese resident species could provide a sufficient protection for non-resident species. The higher toxicity of TCC on aquatic organisms was found compared with other antimicrobial agents (except for Clotrimazole) in aquatic environment. The strong positive linear correlation was observed between the TCC and TCS concentrations in aquatic environment with a correlation coefficient (R²) of 0.8104, it is of great significance in environmental monitoring and risk assessment for TCC and TCS. Finally, the ecological risk assessment showed that the TCC in Yellow River basin and Pearl River basin had higher risk with the mean potential affected fractions (PAFs) of 9.27% and 7.09%, and 22.10% and 15.00% waters may pose potential risk for 5% aquatic organisms, respectively. In general, the risk of TCC in Asian waters was higher than that in Europe and North America.
Show more [+] Less [-]Effect of gibberellic acid on growth, photosynthesis and antioxidant defense system of wheat under zinc oxide nanoparticle stress
2019
Iftikhar, Azka | Ali, Shafaqat | Yasmeen, Tahira | Arif, Muhammad Saleem | Zubair, Muhammad | Rizwan, Muhammad | Alhaithloul, Haifa Abdulaziz S. | Alayafi, Aisha A.M. | Soliman, Mona H.
The production and soil accumulation of nanoparticles (NPs) from the industrial sector has increased concerns about their toxic effects in plants which needs the research to explore the ways of reducing NPs toxicity in pants. The gibberellic acid (GA) has been found to reduce abiotic stresses in plants. However, the effect of GA in reducing zinc oxide (ZnO) NPs-mediated toxicity in plants remains unclear. In this study, foliar application of GA was used to explore the possible role in reducing ZnO NPs toxicity in wheat (Triticum aestivum L.) plants. The plants were grown in pots spiked with ZnO NPs (0, 300, 600, 900, 1200 mg/kg) and GA (0, 100, 200 mg/L) was foliar sprayed at different times during the growth period under ambient environmental conditions. Our results demonstrated that GA inhibited the toxicity of ZnO NPs in wheat especially at higher levels of NPs. The GA application improved the plant biomass, photosynthesis, nutrients, and yield under ZnO NPs stress. The GA reduced the Zn accumulation, and reactive oxygen species generation in plants caused by toxicity of NPs. The protective effect of GA in decreasing ZnO NPs-induced oxidative stress was related to GA-mediated enhancement in antioxidant enzymes in plants. The role of GA in enhancing tolerance of wheat against ZnO NPs was further confirmed by the enhancement in nutrient contents in shoots and roots of wheat. Overall, our study provides the evidence that GA can reduce ZnO NPs-induced toxicity in wheat and probably in other crops which needs further in-depth investigation.
Show more [+] Less [-]Effects of four food dyes on development of three model species, Cucumis sativus, Artemia salina and Danio rerio: Assessment of potential risk for the environment
2019
Motta, Chiara Maria | Simoniello, Palma | Arena, Carmen | Capriello, Teresa | Panzuto, Raffaele | Vitale, Ermenegilda | Agnisola, Claudio | Tizzano, Monica | Avallone, Bice | Ferrandino, Ida
Food dyes, or color additives, are chemicals added to industrial food products and in domestic cooking to improve the perceived flavor and attractiveness. Of natural and synthetic origin, their safety has been long discussed, and concern for human safety is now clearly manifested by warnings added on products labels. Limited attention, however, has been dedicated to the effects of these compounds on aquatic flora and fauna. For this reason, the toxicity of four different commercially available food dyes (cochineal red E120, Ponceau red E124, tartrazine yellow E102 and blue Patent E131) was assessed on three different model organisms, namely Cucumis sativus, Artemia salina and Danio rerio that occupy diverse positions in the trophic pyramid. The evidence collected indicates that food dyes may target several organs and functions, depending on the species. C. sativus rate of germination was increased by E102, while root/shoot ratio was ∼20% reduced by E102, E120 and E124, seed total chlorophylls and carotenoids were 15–20% increased by E120 and 131, and total antioxidant activity was ∼25% reduced by all dyes. Mortality and low mobility of A. salina nauplii were increased by up to 50% in presence of E124, E102 and E131, while the nauplii phototactic response was significantly altered by E102, E120 and E124. Two to four-fold increases in the hatching percentages at 48 h were induced by E124, E102 and E131 on D. rerio, associated with the occurrence of 20% of embryos showing developmental defects. These results demonstrated that the food dyes examined are far from being safe for the aquatic organisms as well as land organisms exposed during watering with contaminated water. The overall information obtained gives a realistic snapshot of the potential pollution risk exerted by food dyes and of the different organism' ability to overcome the stress induced by contamination.
Show more [+] Less [-]Selenium removal and biotransformation in a floating-leaved macrophyte system
2019
Zhou, Chuanqi | Huang, Jung-Chen | Liu, Fang | He, Shengbing | Zhou, Weili
Selenium (Se) is an essential micronutrient for animals with a relatively narrow margin between essentiality and toxicity. To evaluate Se removal efficiency by a constructed wetland treatment system and its potential eco-risk, a floating-leaved macrophyte system was constructed, consisting of three main trophic levels. Over 21-d treatment, water Se concentration was gradually reduced by 40.40%, while 24.03% and 74.41% of the removed Se were found in the plant Nymphoides sp. and sediment, respectively. Among plant tissues, roots accumulated the highest Se level, although the greatest total Se was found in stems, followed by leaves, roots and rhizomes. X-ray absorption spectroscopy revealed that 82.65% of the absorbed selenite by the plants was biotransformed to other forms, as organo-Se species accounted for 45.38% of the Se retained in the sediment, which was primarily responsible for the entry of Se into the detritus food chain. The proportion of organo-Se compounds increased with trophic levels from sediments to fish, indicating, instead of direct uptake of selenite, the food chain transfer and biotransformation of Se may serve as a key exposure route for Se in aquatic organisms. When exposed to organo-Se compounds, i.e., SeCys and SeMet, the plants, shrimp and fish tended to accumulate more Se. However, the greater trophic transfer factor was obtained for selenate, leading to higher Se levels accumulated in fish. Overall, in addition to key mechanisms involved in Se removal, our research also provides a much better understanding of the potential eco-risk that may be posed by the floating-leaved plant system for bioremediation of Se via food chain transfer and biotransformation, paving the way for a low eco-toxic treatment system for Se remediation.
Show more [+] Less [-]Antifouling paint particles in intertidal estuarine sediments from southwest England and their ingestion by the harbour ragworm, Hediste diversicolor
2019
Muller-Karanassos, Christina | Turner, Andrew | Arundel, William | Vance, Tom | Lindeque, Penelope K. | Cole, Matthew
Antifouling paint particles (APPs) of between 500 μm and >2 mm in diameter have been identified in silty, intertidal estuarine sediments through a combination of microscopy and x-ray fluorescence spectrometry. APPs were heterogeneously distributed, with maximal concentrations of 430 particles L−1 (0.2 g L−1) near to a facility where boats are regularly maintained and 400 particles L−1 (4.2 g L−1) at a location where old boats had been abandoned, with the majority of particles encountered in the finest size fraction retrieved. APPs contained variable concentrations of Cu, Zn, Sn and Pb, with respective maxima of 562,000, 269,000, 9,970 and 126,000 mg kg−1. These characteristics are attributed to a multitude of contemporary and historic sources of an assortment of formulations and result in significant but heterogeneous metal contamination of local sediments. APPs were also identified in the guts of the deposit-feeding ragworm, Hediste diversicolor, that inhabited sediments impacted by abandoned boats or boating activities. The tissue of H. diversicolor was particularly enriched in Cu where ingested APPs were observed, with a significant correlation between dry weight Cu concentrations in the two media (r = 0.734) presumably reflecting the inability of the animal to regulate this metal. While the toxicity of APPs requires further investigation, there is clearly a need for stricter regulations on antifouling wastes in boatyards and marinas and a requirement to better manage abandoned boats.
Show more [+] Less [-]A rapid zebrafish embryo behavioral biosensor that is capable of detecting environmental β-blockers
2019
Gauthier, Patrick T. | Vijayan, Mathilakath M.
β-Blockers (BB) are one of the most commonly prescribed pharmaceuticals used for treating cardiovascular and acute anxiety-related disorders. This class of drugs inhibit β-adrenoceptor signalling and given their growing, widespread use, BB are routinely detected in surface waters at nM concentrations. This is concerning as trace levels of BB impart developmental and reproductive dysfunction in non-target aquatic organisms, with potential for ecological risks. To date, environmental pharmaceutical risks to non-target animals are not part of the monitoring framework due to the lack of bioassays for assessing their biological effects. Behavioral endpoints have the advantage of a systems-level integration of multiple sensory signals and motor responses for toxicity screening; however, they are not currently used for risk assessment of environmental contaminants. The zebrafish (Danio rerio) embryo photomotor response (zfPMR) has been used in high-throughput behavioral screenings for neuroactive drug effects at high, therapeutic concentrations. Our objective here was to examine if we could utilize the zfPMR for screening environmental levels of BB. Embryos were placed into 96-well plates, exposed to chemicals and/or municipal wastewater effluent (MWWE), and their zfPMRs were measured with video-analysis. To specifically target BB, embryos were co-treated with isoproterenol, a β-adrenergic agonist that stimulates the zfPMR, and the inhibition of isoproterenol-induced response was used as a biomarker of BB exposure. Our results reveal that the inhibition of isoproterenol-stimulated zfPMRs can be used as a biosensor capable of detecting BB in the parts-per-billion to parts-per-trillion in water samples, including diluted MWWE. The method developed detects BB in spite of the presence of other neuroactive compounds in water samples. This systems level approach of rapid screening for BB effects provides the most promising evidence to date that behavioral neuromodulation can be potentially applied for environmental effects monitoring of pharmaceuticals.
Show more [+] Less [-]Fast visualization of distribution of chromium in rice leaves by re-heating dual-pulse laser-induced breakdown spectroscopy and chemometric methods
2019
Peng, Jiyu | He, Yong | Zhao, Zhangfeng | Jiang, Jiandong | Zhou, Fei | Liu, Fei | Shen, Tingting
Knowledge of distribution of toxic metal in crop is essential for studying toxic metal uptake, transportation and bioaccumulation, and it is important for environmental pollution monitoring. In this study, the macro spatial distribution of chromium in rice leaves was visualized by re-heating dual-pulse laser-induced breakdown spectroscopy (DPLIBS) and chemometric methods. After the optimization of two important parameters (delay time and energy ratio) in DPLIBS, chromium prediction model was established based on global spectra. The global model achieved acceptable performance while slight overfitting for model was found because of numerous irrelevant variables. Feature variables including emissions from chromium and other elements were successfully selected by the values of regression coefficient in partial least square regression model. Best performance was achieved by using the feature variables and support vector machine, with correlation coefficient of prediction of 0.959, root mean square error of prediction of 13.4 mg/kg and residual predictive deviation of 3.6. Finally, the distribution of chromium in rice leaves was visualized with the best prediction model. The distribution image showed that chromium distributed approximately symmetrically along the vein and was likely to be accumulated in leaf apex. The preliminary results provide an approach for investigating the macro spatial distribution of elements in crops, which is important for environmental protection and food safety.
Show more [+] Less [-]First evidence of protein modulation by polystyrene microplastics in a freshwater biological model
2019
Magni, S. | Della Torre, C. | Garrone, G. | D’Amato, A. | Parenti, C.C. | Binelli, A.
Microplastics (MPs) are now one of the major environmental problems due to the large amount released in aquatic and terrestrial ecosystems, as well as their diffuse sources and potential impacts on organisms and human health. Still the molecular and cellular targets of microplastics’ toxicity have not yet been identified and their mechanism of actions in aquatic organisms are largely unknown. In order to partially fill this gap, we used a mass spectrometry based functional proteomics to evaluate the modulation of protein profiling in zebra mussel (Dreissena polymorpha), one of the most useful freshwater biological model. Mussels were exposed for 6 days in static conditions to two different microplastic mixtures, composed by two types of virgin polystyrene microbeads (size = 1 and 10 μm) each one. The mixture at the lowest concentration contained 5 × 105 MP/L of 1 μm and 5 × 105 MP/L of 10 μm, while the higher one was arranged with 2 × 106 MP/L of 1 μm and 2 × 106 MP/L of 10 μm.Proteomics’ analyses of gills showed the complete lack of proteins’ modulation after the exposure to the low-concentrated mixture, while even 78 proteins were differentially modulated after the exposure to the high-concentrated one, suggesting the presence of an effect-threshold. The modulated proteins belong to 5 different classes mainly involved in the structure and function of ribosomes, energy metabolism, cellular trafficking, RNA-binding and cytoskeleton, all related to the response against the oxidative stress.
Show more [+] Less [-]Occurrence, composition profiles and risk assessment of polycyclic aromatic hydrocarbons in municipal sewage sludge in China
2019
Sun, Shao-Jing | Zhao, Ze-Bin | Li, Bo | Ma, Li-Xin | Fu, Dong-Lei | Sun, Xia-Zhong | Thapa, Samit | Shen, Ji-Min | Qi, Hong | Wu, Yi-Ning
A nationwide survey, including 75 sludge samples and 18 wastewater samples taken from different wastewater treatment plants (WWTPs) from 23 cities, was carried out to investigate the occurrence and composition profiles of polycyclic aromatic hydrocarbons (PAHs) in China. In total, the concentrations of ∑16PAHs in sludge ranged from 565 to 280,000 ng/g (mean: 9340 ng/g) which was at a moderate level in the world. The composition profiles of PAHs were characterized by 3- and 4-ring PAHs in textile dyeing sludge and 4- and 5-ring PAHs in domestic sludge. Significant variations in regional distribution of PAHs were observed. Both the principal components analysis and diagnostic ratios revealed that vehicle exhaust, coal and natural gas combustion were the main sources of PAHs in China. The estimated concentrations of PAHs were 3820 ng/L and 1120 ng/L in influents and effluents of the WWTPs, respectively. The high toxic equivalent quantity (TEQ) values of PAHs are ascribed to the high PAH levels. Risk quotient values (RQs) in sludge indicated that there was low potential risk to soil ecosystem after sludge had been applied one year except for indeno [1,2,3-cd]pyrene (IcdP) detected in Huaibei, Anhui province.
Show more [+] Less [-]Cytochrome P450 1A transcript is a suitable biomarker of both exposure and response to diluted bitumen in developing frog embryos
2019
Lara-Jacobo, Linda R. | Willard, Brianna | Wallace, Sarah J. | Langlois, Valerie S.
In order for Alberta's thick bitumen to be transported through pipelines, condensates are added creating a diluted bitumen (dilbit) mixture. Recent pipeline expansion projects have generated concern about potential dilbit spills on aquatic wildlife health. Studies have suggested that polycyclic aromatic compounds (PACs) are toxic to aquatic vertebrates and could potentially also interfere with their endocrine system. The research objectives of this study were to investigate the toxicity of dilbit to developing frog embryos and to identify the molecular mechanisms of action involved. Fertilized embryos of Western clawed frog (Silurana tropicalis) were exposed for 72 h to water accommodated fractions (WAF; 0.7–8.9 μg/L TPACs) and chemically-enhanced WAFs (CEWAF; 0.09–56.7 μg/L TPACs) of Access Western Blend (AWB) and Cold Lake Blend (CLB) dilbits. Both dilbit's CEWAFs significantly increased embryonic mortality and malformation incidence in the highest treatments tested, while WAF treatments led to no visible toxic effects. Increases of the cytochrome P450 1A (cyp1a) mRNA levels were observed for all WAF and CEWAF dilbit treatments suggesting that phase I detoxification is activated in the dilbit-exposed larvae. When exposed to PAC concentrations ranging from 0.09 to 8.9 μg/L, the frogs displayed no observable malformations, but expressed significant increases of cyp1a mRNA levels (2- to 25-fold; indicating a suitable biomarker of exposure); however, when concentrations were of 46.6 μg/L or higher, both malformed frog phenotype and induction of cyp1a mRNA level (>250-fold) were measured (indicating a suitable biomarker of response). The expression of several genes related to cellular detoxification and endocrine disruption were also measured, but were not significantly altered by the treatments. In sum, cyp1a mRNA level is a highly sensitive endpoint to measure subtle molecular changes induced by PAC exposure in the frog embryos and larvae, and data suggest that PAC concentration higher than 46 μg/L would be toxic to the developing S. tropicalis.
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