Gymnodimine A has been found in mollusks obtained along the whole northern coast of Spain from April 2017 to December 2019. This is the first time that this toxin is detected in mollusks from the Atlantic coast of Europe. The prevalence of the toxin was, in general, low, being detected on average in approximately 6% of the obtained samples (122 out of 1900). The concentrations recorded were also, in general, low, with a median of 1.3 μg kg⁻¹, and a maximum value of 23.93 μg kg⁻¹. The maxima of prevalence and concentration were not geographically coincident, taking place the first at the easternmost part of the sampled area and the second at the westernmost part. In most cases (>94%), gymnodimine A and 13-desmethyl spirolide C were concurrently detected, suggesting that Alexandrium ostenfeldii could be the responsible producer species. The existence of cases in which gymnodimine A was detected alone suggests also that a Karenia species could also be involved. The geographical heterogeneity of the distribution suggests that blooms of the producer species are mostly local. Not all bivalves are equally affected, clams being less affected than mussels, oysters, and razor clams. Due to their relatively low toxicity, and their low prevalence and concentration, it seems that these toxins do not pose an important risk for the mollusk consumers in the area.

Known as a cause of food poisoning, Bacillus cereus (B. cereus) is widespread in nature. Cereulide, the heat-stable and acid-resistant emetic toxin which is produced by some B. cereus strains, is often associated with foodborne outbreaks, and causes acute emetic toxicity at high dosage exposure. However, the toxicological effect and underlying mechanism caused by chronic low-dose cereulide exposure require to be further addressed. In the study, based on mouse model, cereulide exposure (50 μg/kg body weight) for 28 days induced intestinal inflammation, gut microbiota dysbiosis and food intake reduction. According to the cell models, low dose cereulide exposure disrupted the intestinal barrier function and caused intestinal inflammation, which were resulted from endoplasmic reticulum (ER) stress IRE1/XBP1/CHOP pathway activation to induce cell apoptosis and inflammatory cytokines production. For gut microbiota, cereulide decreased the abundances of Lactobacillus and Oscillospira. Furthermore, cereulide disordered the metabolisms of gut microbiota, which exhibited the inhibitions of butyrate and tryptophan. Interestingly, cereulide exposure also inhibited the tryptophan hydroxylase to produce the serotonin in the gut and brain, which might lead to depression-like food intake reduction. Butyrate supplementation (100 mg/kg body weight) significantly reduced intestinal inflammation and serotonin biosynthesis suppression caused by cereulide in mice. In conclusion, chronic cereulide exposure induced ER stress to cause intestinal inflammation, gut microbiota dysbiosis and serotonin biosynthesis suppression. IRE1 could be the therapeutic target and butyrate supplementation is the potential prevention strategy.

The townships of Puchuncaví and Quintero, on the coast of central Chile, have soils contaminated by atmospheric deposition of sulfur dioxide and trace elements from the nearby Ventanas Industrial Complex. The purpose of this study was to evaluate potential human health and ecological risks, by determining the spatial distribution of soil total concentrations arsenic (As), copper (Cu), lead (Pb), and zinc (Zn) in these townships. Total concentrations of these elements were determined in 245 topsoil samples, used to generate continuous distribution maps. The background concentrations of Cu, As, Pb, and Zn in the studied soils were 100, 16, 35, and 122 mg kg⁻¹, respectively. The concentrations of Cu, As, and Pb were positively correlated with each other, suggesting that their source is the Ventanas copper smelter. On the other hand, correlations for Zn were weaker than for other trace elements, suggesting low impact of the Ventanas copper smelter on spatial distribution of Zn. Indeed, only 6% of the study area exhibited Zn concentrations above the background level. In contrast, 77, 32 and 35% of the study area presented Cu, As, and Pb concentrations, respectively, above the background level. The carcinogenic risk due to exposure to As was above the threshold value of 10⁻⁰⁴ in the population of young children (1–5 years old) on 27% of the study area. These risk values are classified as unacceptable, which require specific intervention by the Chilean government. Based on the estimated concentrations of exchangeable Cu, 10, 15, and 75% of the study area exhibited high, medium, and low phytotoxicity risk, respectively.

Despite intensive ecotoxicological research, we still know relatively little about the ecological impacts of many environmental contaminants. Filling these knowledge gaps is particularly important regarding amphibians, because they play significant roles in freshwater and terrestrial ecosystems, and their populations are declining worldwide. In this study, we investigated two pollutants that have been poorly studied in ecotoxicology despite their widespread occurrence in surface waters: the herbicide terbuthylazine and the pharmaceutical drug carbamazepine. We exposed two anuran species throughout their larval development to each of two environmentally relevant concentrations of each pollutant, and recorded mortality and 17 sub-lethal endpoints up to several months after exposure. Mortality was low and unrelated to treatment. In agile frogs (Rana dalmatina), we found that treatment with 0.3 μg/L terbuthylazine decreased tadpole activity and reduced fat bodies in juveniles, whereas treatment with 50 μg/L carbamazepine decreased spleen size and increased spleen pigmentation. In common toads (Bufo bufo), treatment with 0.003 μg/L terbuthylazine increased body mass at metamorphosis, treatment with 0.3 μg/L terbuthylazine increased the size of optic tecta, and treatment with 0.5 μg/L carbamazepine decreased hypothalamus size. Treatment with 50 μg/L carbamazepine reduced the feeding activity of toad tadpoles, decreased their production of anti-predatory bufadienolide toxins, and increased their body mass at metamorphosis; juvenile toads in this treatment group had reduced spleen pigmentation. Neither treatments affected the time to metamorphosis, post-metamorphic body mass, or sex ratios significantly. These results show that environmental levels of both terbuthylazine and carbamazepine can have several sub-lethal effects on anurans, which may be detrimental to individual fitness and population persistence in natural conditions. Our findings further highlight that toxic effects cannot be generalized between chemicals of similar structure, because the terbuthylazine effects we found do not conform with previously reported effects of atrazine, a related and extensively studied herbicide.

Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods. Allelochemical interactions are usually quantified through population growth inhibition or lytic-activity based bioassays using a secondary target organism. However these bioassays require time (for growth or microalgal counts) and/or are based on lethal effects. The use of pulse amplitude modulation (PAM) fluorometry has been widely used to assess the impact of environmental stressors on phytoplankton but rarely for allelochemical interactions. Here we evaluated the use of PAM and propose a rapid chlorophyll fluorescence based bioassay to quantify allelochemical BECs released from Alexandrium minutum. We used the ubiquitous diatom Chaetoceros muelleri as a target species. The bioassay, based on sub-lethal effects, quantifies allelochemical activity from different samples (filtrates, extracts in seawater) within a short period of time (2 h). This rapid bioassay will help investigate the role of allelochemical interactions in Alexandrium bloom establishment. It will also further our understanding of the potential relationship between allelochemical activities and other cytotoxic activities from BECs. While this bioassay was developed for the species A. minutum, it may be applicable to other species producing allelochemicals and may provide further insights into the role and impact of allelochemical interactions in forming dense algal blooms and structuring marine ecosystems.

Populations of some species of bats are declining in some regions of Europe. These declines are probably due to a range of pressures, including climate change, urbanization and exposure to toxins such as metals. This paper describes the development, paramaterisation and application of a spatially explicit modeling framework to predict the risks of soil-associated metals (lead, copper, zinc and cadmium) to bat health. Around 5.9% of areas where bats reside were predicted to have lead levels that pose a risk to bat health. For copper, this value was 2.8%, for cadmium it was 0.6% and for zinc 0.5%. Further work is therefore warranted to explore the impacts of soil-associated metals on bat populations in the UK.

Effects of N deficiency and salinity on root anatomy, permeability and metal (Pb, Zn and Cu) translocation and tolerance were investigated using mangrove seedlings of Rhizophora stylosa. The results showed that salt could directly reduce radial oxygen loss (ROL) by stimulation of lignification within exodermis. N deficiency, oppositely, would reduce lignification. Such an alteration in root permeability may also influence metal tolerance by plants. The data indicated that a moderate salinity could stimulate a lignified exodermis that delayed the entry of metals into the roots and thereby contributed to a higher metal tolerance, while N deficiency would aggravate metal toxicity. The results from sand pot trail further confirmed this issue. This study provides a barrier property of the exodermis in dealing with environments. The plasticity of root anatomy is likely an adaptive strategy to regulate the fluxes of gases, nutrients and toxins at root–soil interface.

Blubber samples of Indo-Pacific bottlenose (Tursiops aduncus) and spinner (Stenella longirostris) dolphins from Zanzibar, East Africa, were analyzed for a wide range of organohalogen compounds. Methoxylated polybrominated diphenyl ethers (MeO-BDEs), presumably biogenic, were found at higher concentrations than anthropogenic organochlorine pesticides (OCPs). Only traces of industrial pollutants, such as polychlorinated biphenyls, were detected. The OCP levels found off Zanzibar were lower than those reported from other regions while MeO-BDE levels were higher. The relative composition of the OCPs indicated recent use of lindane (γ-hexachlorocyclohexane) and aged residues of DDT and technical HCH. Placental transfer was estimated to 2.5% and 0.5% of the total burden of OCPs and MeO-BDEs, respectively. Overall transfer from mother to calf in Indo-Pacific bottlenose dolphins was estimated to 72% and 85% for the OCPs and MeO-BDEs burdens, respectively. Health effects of MeO-BDEs are not known, but structural similarities with well-known environmental toxins are cause for concern.

Insect gut microbiotas have a variety of physiological functions for host growth, development, and immunity. Bacillus thuringiensis (Bt) is known to kill insect pests by releasing insecticidal protoxins, which are activated in the insect midgut. However, the interplay among Bt infection, host immunity, and gut microbiota are still unclear. Here we show that Bt Cry1Ac protoxin interacts with the gut microbiota to accelerate the mortality of P. xylostella larvae. Cry1Ac protoxin was found to cause a dynamic change in the midgut and hemocoel microbiota of P. xylostella, with a significant increase in bacterial load and a significant reduction in bacterial diversity. In turn, loss of gut microbiota significantly decreased the Bt susceptibility of P. xylostella larvae. The introduction of three gut bacterial isolates Enterococcus mundtii (PxG1), Carnobacterium maltaromaticum (PxCG2), and Acinetobacter guillouiae (PxCG3) restored sensitivity to Bt Cry1Ac protoxin. We also found that Cry1Ac protoxin and native gut microbiota can trigger host midgut immune response, which involves the up-regulation of expression of Toll and IMD pathway genes and most antimicrobial peptide genes, respectively. Our findings further shed light on the interplay between insect gut microbiota and host immunity under the Bt toxin killing pressure, and this may provide insights for improving the management of Bt resistance and lead to new strategies for biological control of insect pests.

The widespread cultivation of transgenic Bt cotton makes assessing the potential effects of this recombinant crop on non-target organisms a priority. However, the effect of Bt cotton on many insects is currently virtually unknown. The plant bug Adelphocoris suturalis is now a major pest of cotton in southern China and the beetle Haptoncus luteolus is one of the most ancient cotton pollinators. We conducted laboratory experiments to evaluate the toxicity of the Bt cotton varieties ZMSJ, which expresses the toxins Cry1Ac and Cry2Ab, and ZMKCKC, which expresses Cry1Ac and EPSPS, on adult A. suturalis and H. luteolus. No significant increase in the mortality of either species was detected after feeding on Bt cotton leaves or pollen for 7 days. Trace amounts of Cry1Ac and Cry2Ab proteins could be detected in both species but in vitro binding experiments found no evidence of Cry1Ac and Cry2Ab binding proteins. These results demonstrate that feeding on the leaves or pollen of these two Bt cotton varieties has no toxic effects on adult A. suturalis or H. luteolus.