COVID-19 induced pandemic situations have put the bio-medical waste (BMW) management system, of the world, to test. Sudden influx, of COVID-infected patients, in health-care facilities, has increased the generation of yellow category BMW (Y-BMW) and put substantial burden on the BMW-incineration units of India. This study presents the compromising situation of the BMW-incineration units of India, in the wake of COVID-19 pandemic, from 21st March 2020 to 31st August 2020. This analysis revealed that on an average each COVID-infected patient in India generates approximately 3.41 kg/d of BMW and average proportion of Y-BMW in it is 50.44%. Further, it was observed that on 13th July 2020, the total Y-BMW, generated by both the normal and COVID-infected patients, fully utilized the BMW-incineration capacity of India. Also, it was made evident that, during the study period, BMW-incineration emitted several pollutants and their concentration was in the order: NOₓ > CO > SOₓ > PM > HCl > Cd > Pb > Hg > PCBs > Ni > Cr > Be > As. Subsequently, life time cancer risk assessment depicted that with hazard quotient >10⁻⁶, Cd may induce carcinogenic health impacts on both the adults and children of India. Therefore, to mitigate the environmental-health impacts associated with the incineration of BMW, evaluation of various options, viz., alternative technologies, substitution of raw materials and separate treatment of specific wastes, was also done. It is expected that the findings of this study may encourage the global auditory comprising scientific community and authorities to adopt alternate BMW-management strategies during the pandemic.

Plastic pollution in the world's ocean is one of the major environmental challenges that affects the society today, due to their persistence at sea, adverse consequences to marine life and being potentially harmful to human health. Rivers are now widely recognized as being the major input source of land-based plastic waste into the seas. Despite their key role in plastic transportation, riverine plastic pollution research is still in its infancy and plastic sources, hot-spots and degradation processes in riverine systems are to date poorly understood. In this contribution, we introduce a novel concept of following the aging of polypropylene based post-consumer goods placed in known trapping and mobility zones of macroplastics on a fluvial point bar, which was determined through repeated field surveys of macroplastic densities on this bar. As a proof-of-concept, we followed the degradation of 5 identical plastic butter tubs in 5 different locations on a riverbank and significant differences in the aging of the tubs were observed. The degree of aging of the tubs can to some extent be correlated to their proximity to the main river channel, exposure to natural conditions, such as solar radiation, and its storage time on land.

The world's oceans are under increasing pressure from anthropogenic activities, including significant and rapidly increasing inputs of plastic pollution. Seabirds have long been considered sentinels of ocean health, providing data on physical and chemical pollutants in their marine habitats. However, long-term data that can elucidate important patterns and changes in seabird exposure to marine pollutants are relatively limited but are urgently needed to identify and support effective policy measures to reduce plastic waste. Using up to 12 years of data, we examined the benefits and challenges of different approaches to monitoring plastic in seabirds, and the relationship between plastic and body size parameters. We found the mass and number of ingested plastics per bird varied by sample type, with lavage and road-kill birds containing less plastic (9.17–9.33 pieces/bird) than beach-washed or otherwise dead birds (27.62–32.22 pieces/bird). Beached birds therefore provide data for only a particular subset of the population, mostly individuals in poorer body condition, including those severely impacted by plastics. In addition, the mass and number of plastics in beached birds were more variable, therefore the sample sizes required to detect a change in plastic over time were significantly larger than for lavaged birds. The use of lavaged birds is rare in studies of plastic ingestion due to ethical and methodological implications, and we recommend future work on ingested plastics should focus on sampling this group to ensure data are more representative of a population's overall exposure to plastics.

Studies on microplastic (MP) pollution in lakes are recent, although the problem of MP particles in the oceans was first discovered in the 1970s. The first study on lakes was published in 2011. Since then, to our knowledge, 98 lakes have been investigated worldwide. In recent years, studies on this topic have increased worldwide, particularly those focusing on urbanised lakes. Most of the plastic waste in the seas and oceans originates from the terrestrial environment and inland waters. Moreover, lakes are potential temporary or long-term MP accumulators, according to the residence time of water. They are also of high interest for biodiversity, ecology, and the economy. Lacustrine ecosystems may suffer the same fate as marine ecosystems, or even worse, owing to their greater exposure. With the significant focus on ocean and sea contamination, contamination of freshwater ecosystems and lakes is a new and rising topic. However, as a new field of research, several methodological issues have been raised. The team diversity worldwide has led to contrasting sampling techniques and materials, sample treatments, analyses, and presentation of results. Consequently, it is necessary to determine several consensuses between scientific teams in order to work together with accuracy, produce comparable results, speed up knowledge sharing and reduce the reproducibility crisis. This review focuses on (1) MP contamination in 98 worldwide lakes. We identify (2) the theoretical sources of MPs and provide (3) an estimate of MP pollution in different compartments of the lakes based on current state-of-the-art methods. In addition, we also report (4) the predominant MP size classes and polymer types. Finally, we suggest (5) several recommendations to build a consensus between all the working teams to facilitate decision-making by public authorities.

Environmental exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF), one of typical persistent organic pollutants (POPs) produced from municipal waste combustion, exerts toxic effects on human healthy. In the current study, we mainly used targeted metabolomics combined with untargeted ¹H NMR-based metabolomics to investigate the effects of TCDF exposure on lipid homeostasis in mice. We found that TCDF exposure induced hepatic lipogenesis, the early-stage of non-alcoholic fatty liver disease, manifested by excessive lipids including triglycerides, fatty acids and lipotoxic ceramides accumulated in the liver together with elevated serum very low-density lipoprotein by activating the aryl hydrocarbon receptor (AHR) and its target genes such as Cyp1a1 and Cd36. We also found that TCDF exposure induced alteration of phospholipids and choline metabolites and endoplasmic reticulum (ER) markers in the liver of mice, indicating that disruption of host cell membrane structural integrity and ER stress leading to hepatic steatosis. In addition, complementary information was also obtained from histopathologic assessments and biological assays, strongly supporting toxic effects of TCDF. These results provide new evidence of TCDF toxicity associated with fatty liver disease and further our understanding of health effects of environmental pollutants exposure.

Photovoltaic industry has shown tremendous growth among renewable energy sector. Though, this high installation rate will eventually result in generation of large volume of end-of-life photovoltaic waste with hazardous metals. In present study, reported leached metal contents from different photovoltaics in previous investigations were utilized for (i) potential fate and transport analysis to soil and groundwater and, (ii) estimating ecological and human health risks via dermal and ingestion pathways for child and adult sub-populations. The results indicate that the children are at highest risk, mainly due to lead (hazard quotient from 1.2 to 2.6). Metals, such as cadmium, lead, indium, molybdenum and tellurium pose maximum risks for child and adult sub-populations via soil-dermal pathway followed by soil-ingestion pathway. This is further proved by calculated high values of contamination factor and geo-accumulation index for cadmium (102.4), indium (238.9) and molybdenum (16.12). The estimated soil contamination is significant with respect to aluminium, silver, cadmium, iron, lead, however, groundwater contamination was insignificant. Exposure to polluted soils yields an aggregate hazard index (for non-cancer effects) > 1 for all four pathways, with soil dermal pathway as the major contributor. Lead poses significant cancer risk for all scenarios (average risk: 0.0098 to 0.047 (soil) and 2.1 × 10⁻⁵ to 3.5 × 10⁻⁵ (groundwater)), whereas acceptable non-cancer risk was observed for other metals from groundwater exposure. Further, variance contribution and spearman correlation coefficient analysis show that metal concentration, exposure frequency and ingestion rate are the main contributors towards overall uncertainty in risk estimates. More detailed assessment for environmentally-sensitive metals should be carried out by considering other field breakage scenarios also, although the assessment suggests low risk for majority of metals examined.

Marine diatoms have been identified among the most abundant taxa of microorganisms associated with plastic waste collected at sea. However, the impact of nano-sized plastic fragments (nanoplastics) at single cell and population level is almost unknown. We exposed the marine diatom Skeletonema marinoi to model polystyrene nanoparticles with carboxylic acid groups (PS–COOH NPs, 90 nm) for 15 days (1, 10, 50 μg/mL). Growth, reactive oxygen species (ROS) production, and nano-bio-interactions were investigated. No effect on diatom growth was observed, however Dynamic light scattering (DLS) demonstrated the formation of large PS aggregates which were localized at the diatoms’ fultoportula process (FPP), as shown by TEM images. Increase production of ROS and reduction in chain length were also observed upon PS NPs exposure (p < 0.005). The observed PS-diatom interaction could have serious consequences on diatoms ecological role on the biogeochemical cycle of carbon, by impairing the formation of fast-sinking aggregates responsible for atmospheric carbon fixation and sequestration in the ocean sea floor.S. marinoi exposure to PS NPs caused an increase of intracellular and extracellular oxidative stress, the reduction of diatom’s chain length and the adhesion of PS NPs onto the algal surface.

Recent studies have demonstrated the ability of mealworm (Tenebrio molitor) for plastic degradation. This study is focused on changes in microbiome structure depending on diets. Microbial community obtained from oat and cellulose diet formed similar group, two kinds of polyethylene formed another group, while polystyrene diet showed the highest dissimilarity. The highest relative abundance of bacteria colonizing gut was in PE-oxodegradable feeding, nevertheless all applied diets were higher in comparison to oat. Dominant phyla consisted of Proteobacteria, Bacteroides, Firmicutes and Actinobacteria, however after PS feeding frequency in Planctomycetes and Nitrospirae increased. The unique bacteria characteristic for cellulose diet belonged to Selenomonas, while Pantoea were characteristic for both polyethylene diets, Lactococcus and Elizabethkingia were unique for each plastic diet, and potential diazotropic bacteria were characteristic for polystyrene diet (Agrobacterium, Nitrosomonas, Nitrospira).Enzymatic similarity between oatmeal and cellulose diets, was shown. All three plastics diet resulted in different activity in both, digestive tract and bacteria. The enzymes with the highest activity were included phosphatases, esterases, leucine arylamidase, β-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, chitinase, α-mannosidase and α-fucosidase. The activity of digestive tract was stronger than cultured gut bacteria. In addition to known polyethylene degradation methods, larvae may degrade polyethylene with esterase, cellulose and oatmeal waste activity is related with the activity of sugar-degrading enzymes, degradation of polystyrene with anaerobic processes and diazotrophs.

This study examined arsenite [As(III)], arsenate [As(V)] and fluoride (F⁻) removal potential of bone char produced from sheep (Ovis aries) bone waste. Pyrolysis conditions tested were in the 500 °C–900 °C range, for a holding time of 1 or 2 h, with or without N₂ gas purging. Previous bone char studies mainly focused on either low or high temperature range with limited information provided on As(III) removal. This study aims to address these gaps and provide insights into the effect of pyrolysis conditions on bone char sorption capacity. A range of advanced chemical analyses were employed to track the change in bone char properties. As pyrolysis temperature and holding time increased, the resulting pH, surface charge, surface roughness, crystallinity, pore size and CEC all increased, accompanied by a decrease in the acidic functional groups and surface area. Pyrolysis temperature was a key parameter, showing improvement in the removal of both As(III) and As(V) as pyrolysis temperature was increased, while As(V) removal was higher than As(III) removal overall. F⁻ removal displayed an inverse relationship with increasing pyrolysis temperature. Bone char prepared at 500 °C released significantly more dissolved organic carbon (DOC) then those prepared at a higher temperature. The bone protein is believed to be a major factor. The predominant removal mechanisms for As were surface complexation, precipitation and interaction with nitrogenous functional groups. Whereas F⁻ removal was mainly influenced by interaction with oxygen functional groups and electrostatic interaction. This study recommends that the bone char pyrolysis temperature used for As and F⁻ removal are 900 °C and 650 °C, respectively.

The comparison of fly ash generated from lignite combustion in a thermal power plant Kolubara A (Veliki Crljeni) and bottom and fly ash from coal waste combustion in a semi-industrial fluidized bed boiler (Vinča) was performed as the function of particle size. The average total concentrations of the 16 EPA priority PAHs in ash fractions are 0.49 mg kg⁻¹ of ash (thermal power plant) and 17.48 mg kg⁻¹ of ash (fluidized bed boiler). The sum of 3- and 4-ring PAHs accounts for more than 93% of overall PAHs concentration, and the most abundant among them is fluoranthene.The portions of PAHs groups defined based on their physico-chemical properties, as obtained from quantitative structure-activity relationship (QSAR) models included in the Vega platform, were determined. These portions, emission factors, and benzo[a]pyrene equivalence concentrations were further on used to estimate the potential environmental impact of ash disposal. The PAHs emission factors are higher compared to values in the air pollutant emission inventory guidebook of the cooperative program for monitoring and evaluation of the long-range transmission of air pollutants in Europe (EMEP/EEA). The overall emission factors of 16 PAHs for combustion of lignite and coal waste are determined to be 0.15 and 249.97 mg kg⁻¹ of fuel, respectively. Based on the ratios of benzo[a]pyrene equivalence concentrations of each ash and correspondent fuel, the disposal of fly ash from the cyclone of fluidized bed boiler represents the highest risk to the environment among tested ashes.