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Dual effects of nZVI on maize growth and water use are positively mediated by arbuscular mycorrhizal fungi via rhizosphere interactions
2022
Yang, Yu-Miao | Naseer, Minha | Zhu, Ying | Zhu, Shuang-Guo | Wang, Song | Wang, Bao-Zhong | Wang, Jing | Zhu, Hao | Wang, Wei | Tao, Hong-Yan | Xiong, You-Cai
Nanoscale zero-valent iron (nZVI) might generate positive and negative effects on plant growth, since it acts as either hazardous or growth-promotion role. It is still unclear whether such dual roles can be mediated by arbuscular mycorrhizal fungi (AMF) in plant-AMF symbiosis. We first identified that in 1.5 g kg⁻¹ nZVI (≤1.5 g kg⁻¹ positively), maize biomass was increased by 15.83%; yet in 2.0 g kg⁻¹ nZVI, it turned to be declined by 6.83%, relative to non-nZVI condition (CK, p < 0.05), showing a negative effect. Interestingly, the inoculation of AMF massively improved biomass by 45.18% in 1.5 g kg⁻¹ nZVI, and relieved the growth inhibition by 2.0 g kg⁻¹ nZVI. The event of water use efficiency followed similar trend as that of biomass. We found that proper concentration of nZVI can positively interact with rhizosphere AMF carrier, enabling more plant photosynthetic carbon to be remobilized to mycorrhiza. The scanning of transmission electron microscopy showed that excessive nZVI can infiltrate into root cortical cells and disrupt cellular homeostasis mechanism, significantly increasing iron content in roots by 76.01% (p < 0.05). Simultaneously, the images of scanning electron microscopy showed that nZVI were attached on root surface to form an insoluble iron ion (Fe³⁺) layer, hindering water absorption. However, they were efficiently immobilized and in situ intercepted by extraradical hyphae in mycorrhizal-nZVI symbiosis, lowering iron translocation efficiency by 6.07% (p < 0.05). Herein, the optimized structure remarkably diminished aperture blockage at root surface and improved root activities by 30.06% (p < 0.05). Particularly, next-generation sequencing demonstrated that appropriate amount of nZVI promoted the colonization and development of Funneliformis mosseae as dominant species in rhizosphere, confirming the positive interaction between AMF and nZVI, and its regulatory mechanism. Therefore, dual effects of nZVI can be actively mediated by AMF via rhizosphere interactions. The findings provided new insights into the safe and efficient application of nanomaterials in agriculture.
Show more [+] Less [-]Exogenous hesperidin and chlorogenic acid alleviate oxidative damage induced by arsenic toxicity in Zea mays through regulating the water status, antioxidant capacity, redox balance and fatty acid composition
2022
Arikan, Busra | Ozfidan-Konakci, Ceyda | Yildiztugay, Evren | Zengin, Gokhan | Alp, Fatma Nur | Elbasan, Fevzi
Arsenic (As) toxicity is a problem that needs to be solved in terms of both human health and agricultural production in the vast majority of the world. The presence of As causes biomass loss by disrupting the balance of biochemical processes in plants and preventing growth/water absorption in the roots and accumulating in the edible parts of the plant and entering the food chain. A critical method of combating As toxicity is the use of biosafe, natural, bioactive compounds such as hesperidin (HP) or chlorogenic acid (CA). To this end, in this study, the physiological and biochemical effects of HP (100 μM) and CA (50 μM) were investigated in Zea mays under arsenate stress (100 μM). Relative water content, osmotic potential, photosynthesis-related parameters were suppressed under stress. It was determined that stress decreased the activities of the antioxidant system and increased the level of saturated fatty acids and, gene expression of PHT transporters involved in the uptake and translocation of arsenate. After being exposed to stress, HP and CA improved the capacity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), glutathione S-transferase (GST) and glutathione peroxidase (GPX) and then ROS accumulation (H₂O₂) and lipid peroxidation (TBARS) were effectively removed. These phenolic compounds contributed to maintaining the cellular redox status by regulating enzyme/non-enzyme activity/contents involved in the AsA-GSH cycle. HP and CA reversed the adverse effects of excessive metal ion accumulation by re-regulated expression of the PHT1.1 and PHT1.3 genes in response to stress. Exogenously applied HP and CA effectively maintained membrane integrity by regulating saturated/unsaturated fatty acid content. However, the combined application of HP and CA did not show a synergistic protective activity against As stress and had a negative effect on the antioxidant capacity of maize leaves. As a result, HP and CA have great potentials to provide tolerance to maize under As stress by reducing oxidative injury and preserving the biochemical reactions of photosynthesis.
Show more [+] Less [-]The influence of palm oil fuel ash heat treatment on the strength activity, porosity, and water absorption of cement mortar
2022
Shaladi, Ramzi J. | Johari, Megat Azmi Megat | Zainal Arifin Ahmad, | Mijarsh, Mustafa Juma A.
The current study aims to explore the impact of palm oil fuel ash (POFA) heat treatment on the strength activity, porosity, and water absorption of cement mortar. The cement mortar mixtures were typically comprising cement or cement in combination with ultrafine treated POFA (u-TPOFA) which is the final form of the treated POFA, sand, water, and a superplasticizer. Before utilizing the u-TPOFA in mortar mixtures, the treatment processes of POFA were undertaken via five steps (drying at 105 ℃, sieving, grinding, heat treatment, re-grinding) to form u-TPOFA. The heat treatment was performed at three different heating temperatures (i.e., 550 ℃, 600 ℃, and 650 ℃). The ratio on mass/mass basis of the blended ordinary Portland cement (OPC) with u-TPOFA was OPC:u-TPOFA of 70%:30%. A total of four mixtures were prepared, consisting of a plain control mixture (designated as PCM) and three mixtures containing 30% of u-TPOFA treated at three different temperatures designated as M1 “550 ℃,” M2 “600 ℃,” and M3 “650 ℃”. The results show that the optimum mixture was M2 which achieved the highest strength activity index (SAI) of 101.84% and 107% among all mixtures at 7 days and 28 days, respectively. Meanwhile, the porosity (P%) and water absorption (Abs%) of M2 exhibited the lowest values of 9.3% and 4.5%, respectively, among all the mixtures at 28 days. This superior performance of u-TPOFA treated at 600 ℃ represented in the M2 mixture was due to the formation of more binding phases consisting of calcium silicate hydrate (C–S–H) type gel originated from a higher pozzolanic reaction and the filler effects caused by the fine u-TPOFA microparticles. These observations were further confirmed by the improved performance of the M2 mix among all the designed mixes which also exhibited better results in terms of bulk density (BD), ultrasonic pulse velocity (UPV), X-ray diffraction (XRD) as well as thermogravimetry (TGA) and field emission scanning electron microscopy (FESEM-EDX) analyses.
Show more [+] Less [-]Utilizing shale gas drilling cuttings as admixture in cement mortars: a case study in Fuling, Chongqing, China
2022
Huang, Qian | Liu, Yun | Peng, Jingzhi | Xu, Ziyi | Xia, Xin | Deng, Haoling | Li, Hao
Huge amount of water-based drilling cuttings (WDC) is generated during the drilling and exploitation of shale gas. In this study, WDC powder was used as a replacement of cement at different mass fraction (0, 10%, 30%, and 50%) to prepare cement mortars. The mechanical properties, water absorption, and heavy metals leaching of the mortars were then investigated along with the corresponding microstructures. The results showed that a higher porosity was formed within the WDC-blended mortars. For instance, the porosity of the control specimen was 16.7%, while the porosity increased to 20.6%, 21.4%, and 25.0% for the mortars blended with 10%, 30%, and 50% WDC, respectively. Therefore, the mechanical properties of the mortars decreased and their water absorption increased with the increase of WDC replacement ratio. For example, the compressive strength of the mortars blended with 10% and 30% WDC decreased from 33.11 MPa of the control specimen to 29.33 MPa and 21.48 MPa, respectively. Nevertheless, the blended mortars still satisfied the M20 strength grade according to the Chinese standard (JGJ/T 98–2010). The compressive strength of the mortar blended with 50% WDC was 11.33 MPa and then satisfied the M10 strength grade. The leaching test indicated that the water quality of leachates from the mortars blended with lower than 30% WDC remained as Grade I according to the Chinese standard (GB 3838–2002); the water quality of leachate from the mortar blended with 50% WDC was classified as Grade III, which, however, would not cause a safety issue to the environment.
Show more [+] Less [-]Use of waste recycling coal bottom ash and sugarcane bagasse ash as cement and sand replacement material to produce sustainable concrete
2022
Bheel, Naraindas | Khoso, Salim | Baloch, Mereen Hussain | Benjeddou, Omrane | Alwetaishi, Mamdooh
Concrete is widely used as a building material all over the world, and its use is increasing the demand of cement and sand in the construction industry. However, the limited resources and environmental degradation are driving scientists to develop alternative materials from vast volumes of agro-industrial wastes as a partial replacement for conventional cement. In the manufacture of concrete, cement is a major binding resource. This study looked into recycling agro-industrial wastes into cement, such as sugarcane bagasse ash (SCBA), coal bottom ash (CBA), and others, to create sustainable and environmentally friendly concrete. This study aims to see how the combined effects of agricultural by-product wastes affected the characteristics of concrete. SCBA is used to replace fine aggregate (FA) ranging from 0 to 40% by weight of FA, while CBA is used to replace cement content ranging from 0 to 20% by weight of the total binder. In this case, a total of 204 concrete samples (cubes and cylinders) are made using a mixed proportion of 1:1.5:3 and a water-cement ratio of 0.54. Workability, density, water absorption, and mechanical characteristics in terms of compressive and splitting tensile strengths were examined in this study. In addition, for each mix percentage, the total embodied carbon was determined. Workability, density, and water absorption were found to be considerably decreased when CBA and SCBA concentration increased. Due to the pozzolanic nature of CBA and SCBA, an increase in compressive and splitting tensile strengths were seen for specific concrete mixtures, and further addition of CBA and SCBA, the decrease in strength. The embodied carbon of SCBA has augmented the total embodied carbon of concrete, though it can be seen that C15S40, which comprises of 15% CBA and 40% SCBA, is the optimum mix that attained tensile and compressive strength by 3.05 MPa and 28.75 MPa correspondingly, with a 4% reduction in total embodied carbon.
Show more [+] Less [-]Preparation and characterization of modified dual network dust suppression gel based on sodium alginate and soluble starch
2022
Liu, Meiyue | Meng, Qunzhi | Niu, Chenxi | Wang, Yuying | Zhou, Gang | Xu, Cuicui | Liu, Yejiao
Aiming at the problems of complex environment and serious dust pollution in large open-pit coal yards, a dust suppression gel with a dual network structure was prepared by modifying the soluble starch and sodium alginate with iron ions. The changes of functional groups, thermal stability, and morphology structure before and after the reaction were analyzed by FTIR, TG-DSC, and SEM, and the formation mechanism of the dual network was revealed by XPS. Furthermore, the water absorption and water retention experiments proved that the dual network structure is more conducive to water retention than the single-layer network. According to molecular dynamics simulations and contact angle experiments, gel and adsorbed water molecules can approach coal dust molecules on their own to contact, wet, and combine with coal dust. The adhesion test proved that the dust suppression gel with iron ions had better adhesion to dust. The anti-freezing test shows that the dust suppression gel has good anti-freezing performance. The antifreeze test shows that the dust suppression gel still has excellent freeze–thaw resistance at the test temperature of -20℃. The mechanical property test shows that the dust suppressant gel can prevent the product from being damaged by external force. The acid and alkali resistance experiments showed that the acid and alkali resistance of the gel was improved under the condition of iron ion modification, and the flying of coal powder was effectively prevented. This research provides a new theoretical idea for coal dust control in complex environment.
Show more [+] Less [-]Effect of mechanically treated and untreated zinc tailing waste as cement substitute in concrete production: an experimental and statistical analysis
2022
Agrawal, Yash | Gupta, Trilok | Chaudhary, Sandeep
Zinc tailing waste is a type of mine waste generated during the extraction of zinc metal. Disposal of a huge amount of mine tailing waste is an open area and tailing dam causing a negative impact on the natural ecosystem and human health. In this research study, the mechanical properties and durability performance of concrete containing zinc mine tailing waste was investigated through an experimental and statistical analysis. The mechanically treated and untreated zinc tailing waste was used as a cement substitute in concrete production. Concrete specimens were fabricated by replacing cement (0%, 5%, 10%, 15%, and 20%) with the mechanically treated and untreated zinc mine tailing waste. The effect of the zinc mine tailing waste was investigated by conducting the various mechanical (compressive strength and elastic modulus) tests, durability (ultrasonic pulse velocity, water absorption, chloride penetration, carbonation, sulfate attack) tests. The X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM) on concrete samples were also conducted for microstructure analysis. According to the various tests conducted, all concrete properties showed comparable results at the 5% cement substitution in concrete by mechanically treated zinc tailing waste. However, the zinc tailing waste concrete was shown to be more sulphate resistance than the control concrete. Test findings suggest that it is feasible to use 10% mechanically treated and 5% untreated zinc tailing waste as a substitute for cement in concrete to reduce the adverse effect on the environment.
Show more [+] Less [-]The preparation of slow-release fertilizers with biomass ash and water/waste acid solutions from desulfurization and denitrification of flue gas
2022
Qi, Yongfeng | Wang, Ziqian | Ge, Panle | Wang, Meiting | Zhang, Chuanling | Wang, Huawei | Zhao, Lingzhi | Wu, Jiang | Li, Yan
In this study, a method of preparing fertilizers with the fly ash from biomass power plants and the waste acid solution from flue gas desulfurization and denitrification was disclosed. In addition, the study also explored the effects of added fine particles, unburned biochar, and other commercial fertilizers on soil water retention and slow-release effect of fertilizers. The analysis was done by comparing the aggregation degrees of crystalline salt and the variations of the chemical bonds. The experimental results showed that the added fine particles could effectively increase the water absorption of fertilizers, which helped to improve soil water retention. Meanwhile, the fine particles could strengthen the special adsorption of basic compounds containing N, P, and other nutrients by biochar and enhance the slow-release effect of fertilizers. Although adding part commercial fertilizers weakened the water absorption of fertilizers slightly, it had only a relatively small effect on the aggregation of water-soluble crystalline salt on the surfaces of fine particles and biochar. Furthermore, the microwave was applied to promote the absorption of N by unburned biochar, during which only small amounts of volatile were released and lost. The experiments had confirmed that microwave irradiation could promote the agglomeration of biochar on crystalline salt effectively, thus enhancing the slow-release effect of crystalline salt in fertilizers. Finally, pot experiments demonstrated that the self-prepared fertilizer improved plant growth by its better water absorption and slow-release properties during the whole growth period, which had promising application potential as the slow-release fertilizer in the plant growth field.
Show more [+] Less [-]Production of biodegradable pots from cattle manure and wood waste: effects of natural binders on mechanical performances and biodegradability
2022
Manafi-Dastjerdi, Mohammad | Ebrahimi-Nik, Mohammadali | Rohani, Abbas | Lawson, Shaneka
The present study seeks to investigate the use of husbandry waste and sawdust in the construction of degradable pots as a suitable alternative to plastic pots. Six mixture ratios of cattle manure and sawdust (85:15, 80:20, 75:25, 70:30, 65:35, and 60:40) were used along with three types of natural binders (sheep’s wool, cornstarch, and sheep’s wool:cornstarch) in phase I of the project. Phase I was replicated in triplicate to identify the best composition for each binding agent. International standards dictate that evaluations of biological pots include investigations into thickness swelling, internal bonding, and water absorption. Mean comparison of the resultant factorial data using the Tukey and TOPSIS methods indicated that production of bio-pots with a mixture of 80% manure and 20% sawdust may provide the best results for all three pot types. Phase II of the project involved using field experiments and cultivation of tomato plants in direct comparison to a commercial sample pot. The optimal pots for each binding agent in phase I were used in the evaluation. Field tests showed pots produced with 80% manure and 20% sawdust using cornstarch for binding adhesion performed best in terms of degradability and physical and mechanical properties.
Show more [+] Less [-]Effect of silica fume and fly ash as cementitious material on hardened properties and embodied carbon of roller compacted concrete
2022
Kumar, Aneel | Bheel, Naraindas | Ahmad, Israr | Rizvi, Samar Hussain | Kumar, Rabinder | Jhatial, Ashfaque Ahmed
The production of cement releases an enormous amount of CO₂ into the environment. Besides, industrial wastes like silica fume and fly ash need effective utilization to reduce their impacts on the environment. This research aims to explore the influence of silica fume (SF) and fly ash (FA) individually and combine them as binary cementitious material (BCM) on the hardened properties and embodied carbon of roller compacted concrete (RCC). A total of ten mixes were prepared with 1:2:4 mix ratio at the different water-cement ratios to keep the zero slump of roller compacted concrete. However, the replacement proportions for SF were 5%–15%, and FA were 5%–15% by the weight of cement individually and combine in roller compacted concrete for determining the hardened properties and embodied carbon. In this regard, several numbers of concrete specimens (cubes and cylinders) were cast and cured for 7 and 28 days correspondingly. It was observed that the compressive strength of RCC is boosted by 33.6 MPa and 30.6 MPa while using 10% of cement replaced with SF and FA individually at 28 days, respectively. Similarly, the splitting tensile strength of RCC is enhanced by 3.5 MPa at 10% cement replaced with SF and FA on 28 days, respectively. The compressive and splitting tensile strength of RCC is increased by 34.2 MPa and 3.8 MPa at SF7.5FA7.5 as BCM after 28 days consistently. In addition, the water absorption of RCC decreased while using SF and FA as cementitious material individually and together at 28 days. Besides, the embodied carbon of RCC decreased with increasing the replacement level of SF and FA by the mass of cement individually and combined.
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