The stable stabilization of uranium (U) in iron (Fe) containing environments is restricted by the reoxidation of UO₂. In the current study, based on air reoxidation tests, we propose a novel two steps accumulation method to enrich microbial consortia from paddy soil. The constructed microbial consortia, denoted as the Fe–U bacteria, can co-precipitate U and Fe to form stable Fe–U solids. Column experiments running for 4 months demonstrated the production of U(IV)–O–Fe(II) precipitates containing maximum of 39.51% uranium in the presence of Fe–U bacteria. The reoxidation experiments revealed the U(IV)–O–Fe(II) precipitates were more stable than UO₂. 16S rDNA high throughput sequencing analysis demonstrated that Acinetobacter and Stenotrophomonas were responsible for Fe and U precipitation, while, Caulobacteraceae and Aminobacter were crucial for the formation of U(VI)-PO₄ chemicals. The proposed two steps accumulation method has an extraordinary application potential in stable immobilization of uranium in iron containing environments.

Deciphering the potential mechanism of chemical-induced toxicity enables us to alleviate the cellular and organismal dysfunction. The environmental presence of nonylphenol (endocrine disruptor) has a major health concern due to its widespread usage in our day-to-day life. The current study establishes a novel functional link among nonylphenol-induced oxidative stress, Heat shock protein 27 (Hsp27, member of stress protein family), and Ecdysone receptor (EcR, a nuclear receptor), which eventually coordinates the nonylphenol-induced sub-cellular and organismal level toxicity in a genetically tractable model Drosophila melanogaster. Drosophila larvae exposed to nonylphenol (0.05, 0.5 and 5.0 μg/mL) showed a significant decrease in Hsp27 and EcR mRNA levels in the midgut. In concurrence, reactive oxygen species (ROS) levels were increased with a corresponding decline in glutathione (GSH) level and Thioredoxin reductase (TrxR) activity. Increased lipid peroxidation (LPO), protein carbonyl (PC) contents, and cell death were also observed in a correlation with the nonylphenol concentrations. Sub-cellular toxicity poses a negative organismal response, which was evident by delayed larval development and reduced Drosophila emergence. Subsequently, a positive genetic correlation (p < 0.001) between EcR and Hsp27 revealed that nonylphenol-dependent EcR reduction is a possible link for the downregulation of Hsp27. Further, Hsp27 overexpression in midgut cells showed a reduction in nonylphenol-induced intracellular ROS, LPO, PC content, and cell death through the TrxR mediated regenerative pathway and reduced GSH level improving the organismal response to the nonylphenol exposure. Altogether, the study elucidates the potential EcR-Hsp27 molecular interactions in mitigating the nonylphenol-induced cellular and organismal toxicity.

In this work, arsenic (As) accumulation and distribution over time in Pteris vittata young fronds from adult plants and in whole plantlets, grown on a highly contaminated As-soil, was determined by μ-XRF. A linear increase in As content up to 60 days was found in young fronds at different times, and a progressive distribution from the apex to the base of the fronds was observed. In whole plantlets, As signal was detectable from 9 to 20 days in the apex of a few fronds and fiddleheads. Later, up to 60 days, As was localized in all fronds, in the rhizome and in basal part of the roots. The dynamics of expression of As-related genes revealed a good correlation between As content and the level of the As (III)-antiporter PvACR3 transcript in plantlets roots and fronds and in young fronds. Moreover, the transcription of As (V)-related gametophytic genes PvGAPC1, PvOCT4 increases over time during As accumulation while PvGSTF1 is expressed only in roots. Here, we demonstrate the suitability of the μ-XRF technique to monitor As accumulation, which allowed us to propose that As is initially directly transported to fiddleheads and apex of fronds, is later distributed to the whole fronds and simultaneously accumulated in the rhizome and roots. We also provide indications on the expression of candidate genes possibly involved in As (hyper)accumulation.

The emission factor (EF) is a parameter used to assess vehicle emissions. Many studies have reported EFs for vehicles in driving conditions. However, the idling emissions should not be neglected in characterizing actual vehicle emissions in congested large cities, where idling is very common on the road. Whereas, EF data for idling vehicles have scarcely been reported in the literature, let alone comparison of different fuels. In this study, the EFs of passenger cars burning four types of fuels - gasoline, compressed natural gas (CNG), diesel, and liquefied petroleum gas (LPG) were measured and compared. The emissions data for CO, CO₂, unburned hydrocarbon (HC), and NO were recorded to calculate fuel-based EFs in units of g pollutants/kg fuel burned. EFs for CO, HC, and NO were compared for the four fuels. Diesel vehicles had the highest EF for CO, with an average value of 35.12 ± 21.37 g/kg fuel, due to low concentration of CO₂ in lean operation compared to CO emission. CNG vehicles had the highest EF for HC, with an average value of 28.15 ± 11.97 g/kg fuel, due to high concentration of unburned methane gas due to slow CNG flame speed whereas diesel vehicles again had the highest EF for NO due to high temperature and pressure and freezing of NO decomposition reaction, with an average value of 12.07 ± 5.37 g/kg fuel. Further comparison was conducted to analyze the effects of two additional variables on EF: engine displacement volume and model/brand year. Only the gasoline-fueled vehicles showed an increase in EFs (for CO, HC and NO) with the vehicle age according to the model year. However, no clear correlation was observed for CNG, LPG, and diesel-fueled vehicles. Finally, the EF results were compared with those reported in the literature, which have been measured in various countries under both idling and non-idling conditions. Because the idling EFs were not substantially smaller than those under moving conditions, and vehicles spend substantial time idling in large cities, idling emissions should not be ignored in the emission inventories for large cities.

Understanding the impacts of environmental pollutants on immune systems is indispensable in ecological and health risk assessments due to the significance of normal immunological functions in all living organisms. Bivalves as sentinel organisms with vital ecological importance are widely distributed in aquatic environments and their innate immune systems are the sensitive targets of environmental pollutants. As the central component of innate immunity, bivalve hemocytes are endowed with specialized endolysosomal systems for particle internalization and metal detoxification. These intrinsic biological features make them a unique cellular model for metal- and nano-immunotoxicology research. In this review, we firstly provided a general overview of bivalve's innate immunity and the classification and immune functions of hemocytes. We then summarized the recent progress on the interactions of metals and nanoparticles with bivalve hemocytes, with emphasis on the involvement of hemocytes in metal regulation and detoxification, the interactions of hemocytes and nanoparticles at eco/bio-nano interface and hemocyte-mediated immune responses to the exposure of metals and nanoparticles. Finally, we proposed the key knowledge gaps and future research priorities in deciphering the fundamental biological processes of the interactions of environmental pollutants with the innate immune system of bivalves as well as in developing bivalve hemocytes into a promising cellular model for nano-immuno-safety assessment.

Peracetic acid (PAA) as an emerging oxidative has been concerned increasingly due to its high oxidation capacity and low byproducts formation potential. This study was to investigate the oxidation of sulfamethazine (SMZ) by PAA activated with activated biochar (ABC) after thermal modification. The results demonstrated that PAA could be effectively activated by ABC to degrade SMZ in a wide pH range (3–9), which followed the pseudo-second-order kinetics (R² > 0.99). Both non-radicals (singlet oxygen) and free radicals (alkoxy radicals, hydroxyl radicals) existed in the ABC/PAA system, and the degradation of SMZ was dominated by singlet oxygen. Humic acid (HA), SO₄²⁻ and HCO₃⁻ slightly inhibited the degradation of SMZ in the ABC/PAA process, while Cl⁻ and Br⁻ promoted the degradation of SMZ. The cleavage of S–N, S–C bond, and SO₂ extraction reaction rearrangement was the main oxidation process of SMZ. Meanwhile, the results of the ECOSAR program showed that the acute toxicity of most by-products was significantly reduced compared to SMZ, which revealed the potential applicability of the ABC/PAA process in the treatment of antibiotics pollution and their detoxification.

Nonylphenol (Noph) has garnered worldwide concern as a typical endocrine disruptor due to its toxicity, estrogenic properties, and widespread contamination. To better elucidate the interaction of Noph with ubiquitously existing microplastics (MPs) and the potential interdependence of their transport behaviors, batch adsorption and column experiments were conducted, paired with mathematical modeling. Compared with sand, MPs and soil colloids show stronger adsorption affinity for Noph due to the formation of hydrogen bonding and the larger numbers of interaction sites that are available on solid surfaces. Limited amount of soil-colloid coating on sand grains significantly influenced transport behaviors and the sensitivity to solution chemistry. These coatings led to a monotonic increase in Noph retention and a nonmonotonic MPs retention in single systems because of the altered physicochemical properties. The mobility of both MPs and Noph was enhanced when they coexisted, resulting from their association, increased electrostatic repulsion, and competition on retention sites. Limited release of MPs and Noph (under reduced ionic strength (IS) and increased pH) indicated strong interactions in irreversible retention. The retention and release of Noph were independent of IS and solution pH. A one-site model with a blocking term and a two-site kinetic model well described the transport of MPs and Noph, respectively. Our findings highlight the essential roles of coexisting MPs and Noph on their transport behaviors, depending on their concentrations, IS, and physicochemical properties of the porous media. The new knowledge from this study refreshes our understanding of the co-transport of MPs and organic contaminants such as Noph in the subsurface.

The present study aimed to evaluate biochemical and cellular responses of the freshwater mussel, Hyriopsis bialata, to the herbicide atrazine (ATZ). The mussels were exposed to environmentally-relevant concentrations of ATZ (0, 0.02 and 0.2 mg/L) and a high concentration (2 mg/L) for 0, 7, 14, 21 and 28 days. Tissues comprising male and female gonads, digestive glands and gills were collected and assessed for ethoxyresorufin-O-deethylase (EROD) activity, glutathione S-transferase (GST) activity, multixenobiotic resistance mechanism (MXR), histopathological responses, DNA fragmentation and bioaccumulation of ATZ and its transformation derivatives, desethylatrazine (DEA) and desisopropylatrazine (DIA). Additionally, circulating estradiol levels were determined. It appeared that ATZ did not cause significant changes in activities of EROD, GST and MXR. There were no apparent ATZ-mediated histopathological effects in the tissues, with the exception of the male gonads exhibiting aberrant aggregation of germ cells in the ATZ-treated mussels. Contrarily, ATZ caused significant DNA fragmentation in all tissues of the treated animals in dose- and time-dependent manners. In general, the circulating estradiol levels were higher in the females than in the males. However, ATZ-treated animals did not show significant alterations in the hormonal levels, as compared with those of the untreated animals. Herein, we showed for the first time differentially spatiotemporal distribution patterns of bioaccumulation of ATZ, DEA and DIA, with ATZ and DEA detectable in the gonads of both sexes, DEA and DIA in the digestive glands and only DEA in the gills. The differential distribution patterns of bioaccumulation of ATZ and its derivatives among the tissues point to different pathways and tissue capacity in transforming ATZ into its transformation products. Taken together, the freshwater mussel H. bialata was resistant to ATZ likely due to their effective detoxification. However, using DNA damage as a potential biomarker, H. bialata is a promising candidate for biomonitoring aquatic toxicity.

Carbon capture has become an important technology to mitigate ever-increasing CO₂ emissions worldwide, and alkali waste is a potential source of CO₂ capture material. Slagging-gasification is a novel technology for treating municipal solid waste (MSW), and the gasification fly ash (GFA) is the only solid residue that is not reused at present due to its high heavy metal content. GFA contains high amounts of Ca(OH)₂ and Ca(OH)Cl, making it protentional for CO₂ capture. In this study, GFA and washed gasification fly ash (WGFA) were treated with CO₂ for different treatment periods. Weight changes of samples were recorded to evaluate the efficiency of CO₂ capture. To assess the properties of treated GFA, pH value, leached heavy metal concentration, mineral composition, and microscopic morphology were studied. The results revealed that GFA and WGFA could adsorb 18.8% and 23.7% CO₂ of their weights, respectively. Carbonation could immobilize heavy metals including Pb, Zn, and Cu when a proper treatment period was applied. An excessive treatment period decreased the efficiency of heavy metal immobilization. Pre-washing is recommended as a pre-treatment method for GFA carbonation, which increased the efficiency to adsorb CO₂, improved the pH of carbonated GFA, and enhanced the effect to immobilize heavy metals.